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BERITA BIOLOGI

berita_biologi
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Published by Lembaga Ilmu Pengetahuan Indonesia

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Core Subject : Science, Agriculture,

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Articles 11 Documents

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Search results for , issue " Vol 16, No 1 (2017)" : 11 Documents clear

KUALITAS SEMEN BEKU DOMBA GARUT (Ovis ENAMBAHAN SUKROSA DALAM PENGENCER SEMEN KUNING [The Quality of Garut Ram (Ovis Frozen Semen In Tris Egg Yolk Extender to The Sucrose Supplementation] suharman, Herdis
BERITA BIOLOGI Vol 16, No 1 (2017)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

The objective of this research was to examine the effect of sucrose in improving the quality of the plasma membrane intact and sperm motility of frozen semen of Garut ram. Semen was collected using artificial vagina weekly from six mature garut rams. Â Immediately after initial evaluation, fresh semen was divided into four parts and diluted with Tr s extender without sucrose (T0), Tris extender + sucrose 0.2g/100 ml (T1), Tris extender + sucrose 0.4g/100 ml (T2) and Tris extender + sucrose 0.6g/100 ml (T3), respectively. Â Results of this research showed that the percentage of sperm motility after thawing in T2 (49.00 Â± 5.48%) Â was significantly (P<0.05) higher than T0 (42.00 Â± 2.74%) but was not significantly difference (P>0.05) than T1 (46.00 Â± 4.18%)and T3 (48.00 Â± 4.47%). Â Evaluation of plasma membrane intact showed that T1 (62.33 Â± 6.51%) was s gnificantly different (P <0.05) w h T0 (49.40 Â± 2.19%) but was not significantly different (P> 0.05) than T2 (58.50 Â± 4.97%) and T3 (56.40 Â± 5.90%). Â In conclusion, he addition of sucrose in semen extender Â improved the quality of frozen semen of Garut ram. Â Concentration of 0.2g / 100 ml is the op ma dose to improve the quality of the plasma membrane intact and motility of spermatozoa during the freezing process.

KEANEKARAGAMAN KEONG DI PULAU ENGGANO, BENGKULU UTARA [The snails diversity in Enggano Island, Northern Bengkulu] Heryanto, Heryanto
BERITA BIOLOGI Vol 16, No 1 (2017)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v16i1.2240

Research on the diversity of snails in Enggano, Central Bengkulu was held on 16 April 2015 to 5 May 2015 by taking three sampling sites that are north-western part of Enggano (Kampung Bendung at Desa Banjarsari and Desa Meok), the centralpart of Enggano (Desa Â Malakoni) and the south-easternpart of Enggano (Desa Kaana). Sampling was conducted using purposive sampling while the analysis using graphical method and statistical cluster and t-test. This study found 722 specimens of snails which consists of 24 species from 17 families. Most of the snails found are in small size. Land snails in the forest dominates the north-easternpart as much as 88.2%. compared to Â freshwater snails (11.8%). Terrestrial and freshwater snails share amount 50% each in the forest of the central, while terrestrial snails still dominate in the forest of south-eastern part (land snails: snail freshwater = 71.4%: 28.6% ). The existence of land snails and freshwater can not be separated from the state forests themselves besides the condition of the soil and surface water as a result of the topography of the island

INDUKSI BIAK KALUS DAN BIAK SUSPENSI SEL Aquilaria malaccensis Lam. [Induction of Callus Culture and Cell Suspension Culture of Aquilaria malaccensis Lam.] Leksonowati, Aryani; Witjaksono, Witjaksono; Ratnadewi, Diah
BERITA BIOLOGI Vol 16, No 1 (2017)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Aquilaria malaccensis Lam. is a plant species producing fragrant woody material that contains some resin. The compounds can be used as medicine and perfume. Sesquiterpenoid, one group of compounds has been found being synthesized and subsequently extracted from callus and cell suspension culture of Aquilaria species. The aim of this research was to find a method of producing friable calli and cell suspension cultures from leaves or internodes of A. malaccensis in vitro by using suitable plant growth regulators; cell suspension that will suitably serve as material to produce sesquiterpenoid afterwards. Calli were established in almost all treatments of auxin-cytokinin on both leaves and internod explants. The treatment of 10 mg/L IBA induced the highest percentage of callus coverage from leaves with a rather compact structure. The combined treatment of 1â2 mg/L 2.4-D and 0.2â0.3 mg/L BA induced friable callus formation in more than 80% of cultures with 27â32% callus coverage percentage. Â The use of 2,4-D induced a better formation of cell suspension than Picloram, with maximum volume up to 7 mL. Cell suspension culture with fine and homogenous aggregate could be established in the medium supplemented with 0.5 â1 mg/L 2,4-D.

KARAKTERISASI PISANG REJANG TETRAPLOID HASIL INDUKSI DENGAN ORYZALIN [Characterization of tetraploid Pisang Rejang induced by oryzalin] Poerba, Yuyu Suryasari; Handayani, T; Witjaksono, Witjaksono
BERITA BIOLOGI Vol 16, No 1 (2017)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Triploid banana cultivar is the most desirable cultivar in banana industry because of its higher yield compared to its diploid cultivar. Â The Â triploid cultivar can be produced by crossing tetraploid with diploid cultivar. Â However, tetraploid banana cultivar is rarely existed naturally. Â Induced tetraploid of Pisang Rejang was produced using oryzalin. The present research was conducted to characterize tetraploid Pisang Rejang (Musa acuminata, AAAA genome) induced by in-vitro oryzalin treatment from diploid Pisang Rejang. Ploidy level, molecular and morphotaxonomic characters were observed. Â Ploidy identification of induced Pisang Rejang was conducted using Flowcytometer. Â Molecular characterization was done using RAPD and ISSR markers. Morphology characters were observed based on UPOV (2010). The results showed that tetraploid plants have similar genetic properties with their diploid controls as shown by genetic identity of 0.9901 â 0.9935. The tetraploids were differed from their diploid plants in plan habit and diameter of fruit. Â The tetraploid plants produce fewer suckers, drooping leaves and broader fruits compared to its diploid control.

POTENSI SERAPAN CO2 PADA BEBERAPA JENIS KANTONG SEMAR (Nepenthes spp.) DATARAN RENDAH [Potency of CO2 Absorption of Lowland Pitcher Plants (Nepenthes spp.)] Mansur, Muhammad
BERITA BIOLOGI Vol 16, No 1 (2017)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Pitcher plants (Nepenthes spp.) that known as âCarnivorous plantsâ, has a role in CO2 absorption. However, how much contribution to the CO2 absorption has not been studied. This study was aimed to provide information on CO2 absorption of some Nepenthes species particularly lowland species. This study was conducted at Research Center for Biology-Indonesian Institute of Sciences, Â Cibinong Science Center, Cibinong-West Java in June 2014 . A portable LCi ADC Bioscientific Ltd. Photosynthesis System was used to measure the absorption of CO2 directly. Measurement was conducted on 45 individuals of 15 species of Nepenthes with three replications for each species. The results showed that there was a variation of CO2 absorption rate on the lowland Nepenthes species, which was between 3.74 to 12.15 Âµmol m-2 s-1. The highest CO2 absorption was N. mirabilis (12.15 Âµmol m-2 s-1), followed by N. gracilis (9.71 Âµmol m-2s-1) and N. reinwardtiana (9.30 Âµmol m-2 s-1). While the lowest CO2 absorption occured in N. ampullaria (3.74 Âµmol m-2 s-1), N. hispida (3.75 Âµmol m -2 s-1) and N. bicalcarata (4.53 Âµmol m-2 s-1). Mature leaves of each species were recorded to have a highest CO2 absorption, transpiration and chlorophyll content than those of the young leaves. Â Â

CATATAN KEKAYAAN JENIS GASTROPODA DI PESISIR PULAU LETI, KAWASAN BANDA SELATAN [Note on Species Richness of Gastropoda in Coastal Area of Leti Island, Southern Banda] Islami, Muhammad Masrur
BERITA BIOLOGI Vol 16, No 1 (2017)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Note on species richness of gastropods in coastal area of Leti Island, southern Banda was conducted as a part of Southern Banda Expedition on October 7-16, 2015. Sample collection was carried out by hand picking in Serwaru, Tutukey village, Leti Island, Southwest Maluku. Â A total of 125 individu from 25 species of gastropods was found. The highest number of individu is Clypeomorus moniliferus. Generally, gastropods found in this area were associated with rocky hard substrate. Â Â

BAKTERI ENTOMOPATOGEN SEBAGAI AGEN BIOKONTROL TERHADAP LARVA Spodoptera litura (F.) [Entomopathogenic Bacteria as Biocontrol Agent Against Spodoptera litura (F.) Larvae] Zulfiana, Deni; Krishanti, Ni Putu Ratna Ayu; Wikantyoso, Bramantyo; Zulfitri, Apriwi
BERITA BIOLOGI Vol 16, No 1 (2017)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Spodoptera litura (F.) (Lepidoptera: Noctuidae) is one of the agricultural pests that attacking almost all kinds of herbaceous plants, especiallyvegetables. Insect control using entomopathogenic bacteria is an alternative strategy that is effective and has a lower environmentalÂ impact than the use of synthetic insecticides. The purpose of this research was to explore entomopathogenic bacteria that have insecticidalÂ activity against S. litura larvae at various stages of instars. The result showed that 25% of total number of isolated bacteria have potency asÂ entomopathogenic bacteria. Isolate Staphylococcus sciuri strain BLSP-3 and isolate Serratia sp. strain BLSP-4 showed the highest larvicidalÂ activity against the first and second instar larvae of S. litura 83% and 86%, respectively. The activity against on the third instar larvaeÂ however was only by 40%. However, the mortality caused by both isolates was lower than that of Bacillus thuringiensis (more than 90%Â mortality to the first and second instars and 80 % of the third instar larvae). It is suggested that both of isolates are potential to be developedÂ further as a biocontrol agent to control S. litura population.

PENGELOLAAN AIR, BAHAN ORGANIK DAN VARIETAS ADAPTIF UNTUK MENINGKATKAN HASIL PADI DI LAHAN RAWA PASANG SURUT [Water Management, Organic Matter Application and Using Adaptable Variety to Increase Rice (Oryza sativa L.) Productivity on Tidal Swamp Land] Koesrini, Koesrini; Anwar, Khairil
BERITA BIOLOGI Vol 16, No 1 (2017)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Iron toxicity is a factor causing low rice yield on tidal swamp land (TSL).Soil quality improvement by using water management, organic matter and introducing adaptable varietywere some options to increaseits productivity in the soils. Field experiment was conducted to Â evaluate effects of applying water management, organic matter and adaptable variety to increase its productivity on a TSL of KP Belandean, Barito Kuala District of South Kalimantan, during dry season of 2010. The research was arranged in a split-split-plot design with three Â replicates. The main plots were two water management, i.e: P0 = without water management (control) and P1= intermittent water management, while sub plots were three organic matter application, i.e: B0 = without organic matter application (control), B1 = straw Â compost 3 t/ha, B2 = manure 2 t/ha, and sub sub plots were five rice varieties, i.e. V1 = Mekongga, V2 = Inpari 1, V3 = Ciherang, V4 = Silugonggo and V5 = Margasari. The result showed that there was interaction between water management and variety tested on rice yield in TSL. The highest yield was reached by Inpari 1 variety which treatment intermitten water management, with Â yield i.e. 5.390 t/ ha.Silugonggo and Ciherang was not suitable to cultivate on TSL. The implication of this research was variety recomendation on a tidal swamp land, i.e. Inpari 1 variety and intermitten water management.Â

PENINGKATAN PERTUMBUHAN PADI VAR CIHERANG SETELAH DIINOKULASI DENGAN Azospirillum MUTAN MULTIFUNGSI PENAMBAT N2, PELARUT P DAN PENGHASIL FITOHORMON INDOLE ACETIC ACID (IAA). [The growth enhancement of rice var. Ciherang after inoculated with Azospirillum mutants multifunction capable of N2-fixation, P solubilization, and producing phytohormone indole acetic acid (IAA)] Riyanti, Eny Ida; Listanto, Edy
BERITA BIOLOGI Vol 16, No 1 (2017)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Modern agriculture rellies on the application of fertilizer to enhanched plant growth. Excessive application of chemical fertilizers in the long term will decrease soil fertility, therefore the use of biological fertilizers is expected to reduce the negative impact. The aim of this study was to determine the effect of multi-functional Azospirillum for N2-fixation, phosphate solubilizerÃÂ and producing phytohormone Indole Acetic Acid (IAA) on the growth of rice plants Ciherang in the pot experiment conducted in the BB BiogenÃ¢â¬â¢s greenhouse. Two treatments were tested: types of inoculation (not inoculated, were inoculated with the wildtype AjB 6.4.1.2 and inoculated with mutant isolates AJM 3.7.1.14), and 4 levels of fertilizer application (not fertilized, a quarter dose of paddy fertilizer recommendations, a half dose of fertilizer rice in the rice fields, and the appropriate dose of fertilizer in paddy fields). Azospirillum used is wildtype isolate AjB 6.4.1.2 and mutant isolate AJM 3.7.1.14 isolated and and mutated in BB Biogen. Ciherang rice seed inoculated with Azospirillum on the cell density of 106 cells / ml at the different seedlings tub. After the age of 14 days after planting, the seedlings were transferred into pots with three plants per pot. Parameters measured were: plant height, number of tillers, number of panicles per clump, fresh weight and dry panicles per clump, weight of 100 grains, and the content of N and P stover. Results show that inoculantion, affect significantly on the number of panicles per hill, grain weight per panicle and the dry weight of grains per panicle.Â

MIKROBA ENDOFIT DARI TANAMAN SRIKAYA (Annona squamosa L.) SEBAGAI PENGHASIL ANTIMIKROBA Staphylococcus aureus DAN Candida albicans [Antimicrobial activity of endophytic microbes from sugar-apple (Annona squamosa l.) plant againts Staphylococcus aureus and Candida albicans] Melliawati, Ruth; Sunifah, Sunifah
BERITA BIOLOGI Vol 16, No 1 (2017)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Various studies indicated that endophytic microbes lived in the plant tissues and produced antimicrobial compounds. Sugar-apple plant Â (Annona squamosa L) contained alkaloids, cyanogenic glycosides, and flavonoids. The purpose of this reasearch were (1) to determine the endophytic microbes isolated from sugar-apple plant (2) to study inhibiting capabillity of endophytic isolate against Staphylococcus aureus and Candida albicans, (3) to analyze antimicrobial compounds produced by the potential endophytic isolate. Diffusion agar plate methode was used to assessed antimicrobial activity. Antimicrobial compounds were analyzed by Thin Layer Chormatography (TLC) and High Performance Liquid Chormatography (HPLC), compared with erythromycin, metronidazole and tetracycline. Twelve bacterial isolates and 24 fungus were isolated. Selected bacteria, BMC 1.1, showed the biggest clear zone on C. albicans culture on agar medium, meanwhile selected fungi, BTCK 1.1T, formed the biggest colony on S. aureus culture on agar medium. TLC and HPLC analysis showed that the Rf value of BMC 1.1 and BTCK 1.1T chloroform phase fractions was similiar to metronidazole. Metronidazole concentration in C1, C2, Ck1 and Ck2 fraction were 170.98 ppm, 18.27 ppm, 1.51 ppm and 4.14 ppm respectively.

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