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International Journal of Applied Biology
Published by Universitas Hasanuddin
ISSN : 25802410     EISSN : 25802119     DOI : -
International Journal of Applied Biology is an international, peer reviewed journal, publishing high-quality research. International Journal of Applied Biology only publishes manuscripts in English. Please see the journal's Focus & Scope for information about its Scope and peer-review policy. International Journal of Applied Biology is available in print and online version.
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Articles 5 Documents
Search results for , issue "Vol. 1 No. 2 (2017): International Journal of Applied Biology" : 5 Documents clear
Effect of Yeast Based Biofertilizer combined with bacteria on Mustard Plant Growth Nur Hidayatul Alami
International Journal of Applied Biology Vol. 1 No. 2 (2017): International Journal of Applied Biology
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/ijab.v1i2.3093

Abstract

Biofertilizers are commonly defined as microbial inoculants which are capable of mobilizing important nutrient in the soil from non-available to available form for the plants through their biological processes. Hence to increase the productivity of the soil, the use of biofertilizer is necessary. Candida yeast as a phosphate solubilizer and decomposer. Azotobacter bacteria as a nitrogen-fixing bacteria and Bacillus as a phosphate solubilizer. The combination of these microorganisms as biofertilizer needs to be tested its effect against mustard (Brassica juncea L.) plant growth.The results showed that the combination of Bacillus Candida obtained the highest yield among other biofertilizer treatments with a value of 13.6 cm at plant height, 4.96 grams in wet weight and 2.29 grams in dry weight. The combination of Azotobacter Candida showed the highest result on the parameter of width and number of leaves with the values of 20.3 cm and 5.3. Combination of Azotobacter Bacillus Candida showed the highest yield on the root length parameter with a value of 5.0 cm. The addition of biofertilizer is expected to reduce pollution on the soil due to residues produced by chemical fertilizers.Keyword : Azotobacter, Bacillus, Biofertilizer, Candida, Mustard Plant
Study of Lacid Acid Bacteria Activities from Human Oral Mucosa for Candida albicans Inhibition Evi Lauw
International Journal of Applied Biology Vol. 1 No. 2 (2017): International Journal of Applied Biology
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/ijab.v1i2.2261

Abstract

Lactic acid bacteria are known to have potential in producing antimicrobial compounds, including antifungal agents. This study aims to determine whether the lactic acid bacteria from human oral mucosa have the potential to produce the secondary metabolite to inhibit the candidiasis, which caused by pathogen fungi, Candida albicans. Lactic acid bacteria were isolated from three healthy respondents based on age difference that is an infant, child, and adult. Lactic acid bacteria isolation using the deMann Rogosa Sharpe Broth (MRSB)-liquid medium, followed by purification stage with the quadrant scratch method using the deMann Rogosa Sharpe Agar (MRSA)-solid medium. The purified isolates obtained were characterized and identified by Gram staining, biochemical tests, and inhibition zone diameter measurement toward pathogenic fungi Candida albicans. The results were obtained from each of the two lactic acid bacteria isolates from each respondent with characteristics including gram-positive bacteria with short stem cell shape, possibly as Lactobacillus sp. It is conclude that the lactic acid bacteria of oral mucosa from adult had greater anticandidal activity than infant and child. Keywords: lactic acid bacteria, human oral mucosa, antifungal activity, Candida albicans
Talinum paniculatum (Jacq) Gaertn (Java ginseng) production using Vesicular-Arbuscular Mycorrhizal Sulistiono Sulistiono
International Journal of Applied Biology Vol. 1 No. 2 (2017): International Journal of Applied Biology
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/ijab.v1i2.3133

Abstract

Soil pathogen is one of main problem in Java ginseng production in tropical field. This study was aimed to evaluate the impact of VAM application to Java ginseng tuber production. Application of VAM was conducted in various concentrations (0, 1, 2, and 3%) (w/w). Thin Layer Chromatography was used to detected saponin and spectrophotometry was used to determine total saponin. Biomass, the number of rotten tuber, and total saponin were determined after eleven months under greenhouse condition. Inoculation 3% of VAM can enhance the number of healthy and biomass Java ginseng tuber significantly. But the application of VAM did not increase total saponin content.
The Analysis of β-cryptoxanthin and Zeaxanthin using HPLC in the Accumulation of Orange Color on Lowland Citrus Inanpi Hidayati Sumiasih; Roedhy Poerwanto; Darda Efendi; Andria Agusta; Sri Yuliani
International Journal of Applied Biology Vol. 1 No. 2 (2017): International Journal of Applied Biology
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/ijab.v1i2.3066

Abstract

Citrus peel color is one of the main quality attributes which was caused by the accumulation of carotenoids and its derivatives, especially β-citraurine. It makes citrus peel color looks attractive (orange). The orange color is a mixture of β-cryptoxanthin with β-citraurin. The objectives of this study were (1) to observe the effect of precooling and duration of proper ethylene exposure in the formation of orange color on citrus peel, (2) to identify and determine the β-cryptoxanthin content and total chlorophyll on citrus peel. Citrus was from Tuban, East Java while the study was conducted at PKHT IPB and LIPI. Precooling and without precooling treatment prior to injection of 100 ppm of ethylene exposed at 15 °C, duration of exposure control (0), 24, and 48 hours. The results show that the best color of the Citrus Color Index (CCI) is the precooling treatment and the duration of ethylene exposure for 24 hours, which can reduce total chlorophyll content about 8 times and proved to increase β-cryptoxanthin pigment content five times in accelerating the formation of orange citrus reticulata peel color to bright orange. Degreening has no significant effect on total dissolved solids and the firmness level of citrus fruits.Keyword: β-cryptoxhantin; citrus; chlorophyll; degreening; ethylene zeaxanthin.
Biodiversity and distribution of gastropods at seagrass meadow of Balangdatu waters Tanakeke Island South Sulawesi Indonesia Magdalena Litaay; Marwa Deviana; Dody Priosambodo
International Journal of Applied Biology Vol. 1 No. 2 (2017): International Journal of Applied Biology
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/ijab.v1i2.3134

Abstract

The research about the biodiversity of gastropod has been conducted in seagrass meadow of Balangdatu waters, Tanakeke Island, South Sulawesi. The research aims to assess the diversity of gastropod species in Balangdatu waters. Sampling was conducted using quadrate transect method systematically. Three replicates of transect were applied for each station. The result indicates there were 34 species of gastropods from 14 genera and 14 families were found. Diversity index from every station varies from 1,661 to 2, 899. These values range from low to moderate. The diversity, Evenness, and dominance indices showed that Balangdatu waters still in good condition and sustain habitat for gastropod.

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