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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences JURNAL GIZI INDONESIA
Dewijanti, Indah Dwiatmi
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Agriculture, Biological Sciences & Forestry Environmental Science Health Professions

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Antiviral Effect of Pterocarpus indicus Willd Leaves Extract Against Replication of Dengue Virus (DENV) In Vitro Dewi, Beti Ernawati; Angelina, Marissa; meilawati, lia; Hartati, Sri; Dewijanti, Indah Dwiatmi; Santi, Mei Ria; Desti, Hidayati; Sudiro, Mirawati
Journal of Tropical Life Science Vol 8, No 1 (2018)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (886.48 KB) | DOI: 10.11594/jtls.08.01.10

Abstract

Dengue hemorrhagic fever (DHF) is major public health problem in tropical and subtropical areas of the world with lack of approved vaccines and effective antiviral therapies. With no current treatment for illness attributed to dengue virus (DENV) infection other than supportive care, therapeutic strategies that use natural extract was developed. Indonesia have many plants that potential for antiviral drµgs such as Pterocarpus indicus Willd (P. indicus). The objective of this study was to determine the effect of P. indicus to inhibit DENV replication. We used a well-differentiated hepatocytes-derived cellular carcinoma cell line (Huh-7 it-1 cells) to determine and select antiviral activity. The toxicity effects were determined by MTT assay. Then, the suppression of DENV replication was determined by Focus assay. Dengue infected cells with DMSO were used as control. We found that crude extract (Pi), hexane (Pi.1) and ethyl acetate (Pi.2) extract showed strong inhibition with high selectivity index (SI) of 1,392; 285.36 and 168.56 respectively.  Sub fraction of Pi.1 and Pi.2 still showed strong inhibition with high SI.  Further sub-sub fraction of Pi.2 such as Pi.2.12 and Pi.2.12.1 still showed inhibition of DENV replication but there was reduction of SI value. The mechanism experiment of Pi.2.12, we found that Pi 2.12 more profound to inhibit in the post infection stage that entry or pre-infection. We conclude that the sub-fraction of Pi.2.12 has potential antiviral activity against DV infection in vitro. Further studies are still needed to investigate the pure compound of Pi.2.12 that inhibit and have advantages in the future as alternative for treatment of DENV infection.
Toxicity and Lethality Evaluations of Koordersiodendron pinnatum Leaves Methanolic Extract in DDY Mice Banjarnahor, Sofna Dewita Sari; Dewijanti, Indah Dwiatmi; Angelina, Marissa; Fajriah, Sofa
Journal of Tropical Life Science Vol 6, No 3 (2016)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.06.03.10

Abstract

The study of toxic features of Koordersiodendron pinnatum is vital for further studies of its pharmacological activities. Acute toxicity test was done on methanolic extracts of K. pinnatum in DDY mice. Animals were grouped into five: Group 1 was given 1 mL solution of 2.5% Tween 80 in a single oral dose; the remaining groups were orally given a single dose of 2, 4, 8 and 16 g/kg of K. pinnatum, respectively. Toxic effects of the extract were evaluated on the basis of behavioral observations in the form of locomotor activity; curiosity; defecation; urination and also animal mortality. Observations were carried out for 14 days. No significant changes on body weight, and behavioural activities were recorded. Mortality was recorded up to 2% of the male group, and no mortality within the female group. The extract is practically non toxic for both male and female (LD50>15 g/kg).
In vitro lipase enzyme inhibitory activities of green tea and other herbs Megawati, Megawati; Artanti, Nina; Mulyani, Hani; Darmawan, Akhmad; Syahrian, Heri; Lotulung, Puspa Dewi N.; Supriadi, Edi; Widiyarti, Galuh; Dewi, Rizna Triana; Meilawati, Lia; Ernawati, Teni; Dewijanti, Indah Dwiatmi; Minarti, Minarti
Jurnal Gizi Indonesia (The Indonesian Journal of Nutrition) Vol 9, No 1 (2020)
Publisher : Department of Nutrition Science, Faculty of Medicine, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jgi.9.1.48-52

Abstract

Background: Increased lipase activity can increase the amount of monoglycerides and fatty acids absorbed by the body, this is what affects obesity. If pancreatic lipase activity is inhibited, the production of fatty acids will decrease, consequently the level of fat in the blood will also decrease.Objective: To study anti obesity potential of a single herb or a mixture of green tea and other herbs (Syzygium polyanthum. Lv, Artocarpus communis.Lv, Cinnamomum verum..Lv, Manilkara zapota.Lv, Tectona grandis.Lv) by the in vitro inhibition assay of the lipase enzyme activityMethod: A single (8 herbs) or a mixture of three herbs powder (12 formulas) was put into a tea bag with a total weight of 1.5 g to be brewed in 100 mL bottled mineral water at 70⁰-90⁰C for 10 minutes. In vitro lipase enzyme inhibition assay were conducted to measure the ability of these samples as lipase inhibitor. Orlistat used as positive lipase inhibitor. Results: There was a difference results of lipase inhibition activity between the sample of a single and a mixture of herbs. Interestingly, the results shows that formula 3 (mixture of green tea, Manilkara zapota and cinnamon). has 53.942 % inhibition, formula 9 (a mixture of green tea, Syzygium polyanthum and cinnamon) has 67.322 % inhibition and formula 12 (a mixture of green tea, Tectona grandis and cinnamon) has 56.612 % inhibition which close to lipase inhibitory activity by standard Orlistat.Conclusion: The mixture of green tea and two other herbs has a lipase inhibitory activity similar to Orlistat, when compared to the single herb. The highest lipase inhibitory activity is found in formula 9.
Co-Authors Akhmad Darmawan BETI ERNAWATI DEWI Desti, Hidayati Galuh Widiyarti Hani Mulyani, Hani Heri Syahrian, Heri Lia Meilawati Lotulung, Puspa Dewi N. Marissa Angelina Megawati - Minarti Minarti Mirawati Sudiro Nina Artanti Rizna Triana Dewi Santi, Mei Ria Sofa Fajriah Sofna Dewita Sari Banjarnahor, Sofna Dewita Sari Sri Hartati Supriadi, Edi Teni Ernawati