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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences JURNAL GIZI INDONESIA Medical Journal of Indonesia Jurnal Bioteknologi & Biosains Indonesia (JBBI) ANNALES BOGORIENSES Makara Journal of Science Makara Journal of Technology
Nina Artanti
Research Center of Chemistry, Indonesian Institute of Scinces, Kawasan Puspiptek Serpong, Tangerang 15314, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Electrical & Electronics Engineering Engineering Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Mechanical Engineering Medicine & Pharmacology

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Antioxidant properties of flavonoids Banjarnahor, Sofna D.S.; Artanti, Nina
Medical Journal of Indonesia Vol 23, No 4 (2014): November
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (701.645 KB) | DOI: 10.13181/mji.v23i4.1015

Abstract

Flavonoids represent a remarkable group of plant secondary metabolites and have long been used as traditional medicines with scientifically proven pharmacological benefits. They serve vast-ranging medicinal activities that may lead drug discovery with novel and potential therapeutic evidence. Latest research magnifies primarily functional activity of flavonoids as antioxidant against oxidative stress. This review enlightens the prospective role of flavonoids as antioxidant.
Pcr Amplification of Ornithine Decarboxylase (ODC) Gene Fragment from Tobacco (Nicotiana tabacum L.) cv. Temanggung Djajanegara, Ira; Pambudi, Sabar; Lestari, Retno; Artanti, Nina
ANNALES BOGORIENSES Vol 9, No 2 (2004): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.1234/11

Abstract

In order Lo create an antisense construct of the gene encoding Ornithine Decarboxylase (ODC) from tobacco (Nicotiana tabacum L.) cv. Temanggung, the target gene must be isolated. In this paper. we present the PCR amplification of a fragment from putative gene encoding ODC from tobacco cv. Temanggung. Leaf genomic DNA was i olated and used as the template for PCR. PCR optimization was done by adjusting the annealing temperature and the cycle number. Verification of the fragment obtained was also done using the second primer pairs .  
Isolation and Identification of Antiplasmodial Compound from Methanol Extract of Calophyllum bicolor P. F. Steven Abbas, Jamilah; Hanafi, Muhammad; Artanti, Nina; Sundowo, Andini; Minarti, Minarti; Asih, Puji Budi Setia; Syafrudin, Din
ANNALES BOGORIENSES Vol 19, No 2 (2015): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/ab.v19i2.253

Abstract

Calophyllum bicolor (Clusiacea) is a big tree from Indonesian rain forest in Palangkaraya, Central Kalimantan. Calophyllum or bintagor is one of many sources of natural bioactive compounds that can be used in the fields of health and pharmaceuticals. The aim of this research was to explore the antiplasmodial activity of methanol extract of Calophyllum bicolor P.F. Steven against Plasmodium falciparum. The methanol extract was purified by colomn chromatography system, hexane – ethyl acetate was used as solvent with increasing polarity. One pure compound was obtained and was elucidated based on the 1H-&13C-NMR and 2D-NMR, [COSY, HMBC and HMQC] data and the isolated compound was identified as xanthone. Methanol extract showed antiplasmodial activity growth inhibition against P. falciparum with IC50 5.2 ppm and the new 5-methoxy trapezifolixanthone compound have maximum inhibition at concentration 0.11 nMol.
Bioactivities Screening of Indonesian Marine Bacteria Isolated from Sponges Artanti, Nina; Maryani, Faiza; Mulyani, Hani; Dewi, Rizna Triana; Saraswati, Vienna; Murniasih, Tutik
ANNALES BOGORIENSES Vol 20, No 1 (2016): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (148.451 KB) | DOI: 10.14203/ab.v20i1.234

Abstract

The marine bacteria were cultured in liquid medium under shaking condition were extracted with ethyl acetate. Antidiabetes was measured using inhibition of α-glucosidase inhibitory activity method; antioxidant was measured using DPPH free radical scavenging activity method; antibacterial was tested using disc diffusion method.S creening results showed that at sample concentration of 200 µg/ml, there was significant α-glucosidase inhibitory activity was detected in the extracts of strain sp 7.9 (84% inhibition) and 8.10 (75% inhibition),however the antioxidant activity of these two strains were low only around 30% inhibition, antioxidant activities of other strains were very low.Screening for antibacterial activities using 10µl samples showed that extract of strain Sp 8.5was best for Staphylococcusaureus (14 mm inhibition); Sp 7.9 and Sp 8.5 for Bacillus subtilis (18 mm inhibition); Sp 8.10 for Escherichia coli (10 mm inhibition); Sp 8.9 and Sp 8.10 for Pseudomonas aeuriginosa. Based on these results marine bacteria strain Sp 7.9 and Sp 8.10 were selected to be used for further studies in the isolation of bioactive that has potential as antidiabetes and antibacterial.Results of molecular identification conducted by INACC showed that identity of both strain based on BLAST Homology using NCBI database were Bacillus thuringiensis strain Ou2.
Isolation of cDNAs Encoding Asparagine Synthetase from Coronilla rostrata Artanti, Nina; Mcfarlane, Ian James
ANNALES BOGORIENSES Vol 16, No 1 (2012): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (609.798 KB) | DOI: 10.1234/39

Abstract

Asparagine synthetase (AS) is one of the enzyme involves  in ammonium metabolism in plant, it catalyzes the transfer of the amino group of glutamine to aspartate giving asparagine. Asparagine serves as a major nitrogen transport and storage molecule in many higher plants. This research used legume plant Coronilla rostrata callus culture for primary and secondary metabolism study. On primary metabolism, the culture were used to study the phenomenon of asparagine accumulation. Because of the responsiveness of the culture, we  investigated the use of this culture for model system to conduct molecular biology study of the nitrogen metabolism. The aim of this study was to isolate...cDNAs enconding  Asparagine synthetase  from  C. rostrata because of the asparagine accumulate in this culture.  Several PCR based approaches  were conducted such as RT-PCR, LM-PCR and RACE. Using these methods, two AS cDNAs was  isolated from C. rostrata. AS1 (GenBank no AY081945) which a complete cDNA sequence and AS2 which a partial cDNA sequence.  (GenBank no AF488726). Thesetwo cDNAs had high homology with the legume AS. Keywords: cDNA, nitrogen metabolism, asparagine synthetase, Coronilla rostrata
Bioactivity of Antibacterial Compounds Produced by Endophytic Actinomycetes from Neesia altissima Pratiwi, Rina Hidayati; Hanafi, Muhammad; Artanti, Nina; Pratiwi, Rosa Dewi
Journal of Tropical Life Science Vol 8, No 1 (2018)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (835.918 KB) | DOI: 10.11594/jtls.08.01.07

Abstract

Identification of alternate bioactive compounds isolated from microbial endophytes from plants may lead to better solution against antibiotic resistance. Main objective of this research is to determine the bioactivity of antibacterial compounds produced by endophytic actinomycetes, which was isolated from stem bark of Neesia altissima Bl. at Halimun Salak Mount. Detection of bioactive compounds was assayed using Thin Layer Chromatography (TLC) by employing bioautography. Bioactivity was assayed using crude extract against five microorganisms using agar diffusion methods. Tetracycline was used as a positive control. The result of antibacterial activity from endophytic actinomycetes isolate revealed that the bioactive compounds were effective in inhibiting the growth of bacteria Bacillus cereus (1.25 mm), Salmonella typhimurium (5.75 mm), and Shigella flexneri (4.37 mm). Bioactivity of ethyl acetate extract from bioactive compounds of endophytic actinomycetes has an antibacterial activity against bacteria Bacillus cereus (13.00 mm) and Staphylococcus aureus (15.90 mm), at a concentration of 250,000 ppm. Each of TLC fraction was able to inhibit growth of B. cereus at a minimum concentration of 10,000 ppm. In conclusion, the bioactive compounds produced by endophytic actinomycetes can have inhibitory effect towards B. cereus. The category of that compounds are narrow spectrum. The bioactive compound isolated from endophytic actinomycetes may have other implications such as anticancer, antifungal, and antioxidant.
PENGARUH WAKTU FERMENTASI TERHADAP AKTIVITAS ANTIOKSIDAN DAN KADAR BETASIANIN MINUMAN FUNGSIONAL DARI BUAH NAGA (Hylocereus polyrhizus) DAN UMBI BIT (Beta vulgaris) Maryati, Yati; Susilowati, Agustine; Artanti, Nina; Lotulung, Puspa Dewi Narij; Aspiyanto, Aspiyanto
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 7 No. 1 (2020): June 2020
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (716.82 KB) | DOI: 10.29122/jbbi.v7i1.3732

Abstract

Effect of Fermentation on Antioxidant Activities and Betacyanin Content of Functional Beverages from Dragon Fruit and BeetrootKombucha is a traditional beverage prepared by fermenting polyphenol-rich tea using a consortium of bacteria and yeast (SCOBY). Dragon fruit and beetroot contain a significantly high amount of polyphenols and betacyanin as antioxidants which are beneficial in reducing the risk of cardiovascular disease, cancer, and the body's natural degeneration related to the early-aging process. This study aims to determine changes in antioxidant activity and levels of betacyanin from fermented dragon fruit and beetroot as functional drinks during different fermentation times. The results showed that there was a correlation between fermentation time and antioxidant activity in its ability to counteract free radicals, ferric reducing antioxidant power (FRAP), and the levels of betacyanin produced. Longer fermentation time caused an increase in free radical inhibition and reduction of iron ions but reduced the levels of betacyanin. The optimum value of free radical inhibitory activity (DPPH) was obtained in the 12-day fermented dragon fruit drink with an inhibitory value of 80.76%, ability to reduce iron ions by 197.94 µg ascorbic acid mL-1, and betacyanin level of 0.055 mg L-1Keywords: antioxidants, dragon fruit, betacyanin, beetroot, functional beverages ABSTRAKKombucha merupakan minuman tradisional hasil olahan fermentasi teh yang kaya polifenol dengan konsorsium bakteri dan yeast (SCOBY). Buah naga dan umbi bit memiliki polifenol dan senyawa betasianin yang cukup tinggi sebagai antioksidan yang bermanfaat dalam menurunkan risiko penyakit kardiovaskular, penyakit kanker dan degenerasi alami tubuh terkait proses penuaan dini. Penelitian ini bertujuan untuk mengetahui perubahan aktivitas antioksidan dan kadar betasianin dari fermentasi buah naga maupun umbi bit sebagai minuman fungsional selama waktu fermentasi yang berbeda. Hasil penelitian menunjukkan bahwa adanya korelasi antara waktu fermentasi terhadap aktivitas antioksidan dalam kemampuannya menangkal radikal bebas, kemampuan mereduksi ion besi (FRAP), dan kadar betasianin yang dihasilkan. Semakin lama waktu fermentasi menyebabkan peningkatan penghambatan radikal bebas dan reduksi ion besi, namun menurunkan kadar betasianin. Nilai aktivitas penghambatan radikal bebas (DPPH) optimum diperoleh dari minuman fermentasi buah naga selama waktu fermentasi 12 hari dengan nilai penghambatan sebesar 80,76%, kemampuannya dalam mereduksi ion besi sebesar 197,94 µg asam askorbat mL–1, dan kadar betasianin sebesar 0,055 mg L–1
Isolation of an Anti-Cancer Asperuloside from Hedyotis corymbosa L. Artanti, Nina; Hanafi, Muhammad; Andriyani, Rina; Saraswaty, Vienna; Udin, Linar Zalinar; Lotulung, Puspa D; Fujita, Ken Ichi; Usuki, Yoshinosuke
Journal of Tropical Life Science Vol 5, No 2 (2015)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.05.02.06

Abstract

Hedyotis corymbosa, with local name rumput mutiara, is an anti-inflammatory, anti-cancer and hepatoprotective traditional medicine.  The ethanol extract of H. corymbosa L. shows inhibitory activity to human YMB-1 breast cancer cell line with an IC50 of 6.51 mg/ml.  The methylene chloride fraction shows a potential cytotoxic activity with an IC50 of 2.75 mg/ml.  To obtain a lead compound, the extract was further purified by column chromatography. A pure compound is obtained which shows inhibitory activities against YMB-1, HL60 and KB human cell lines with IC50 values of 0.7; 11.0 and 104.2 mg/ml, respectively.  Based on the 1D and 2D FT-NMR data, the isolated compound is an asperuloside.
In vitro lipase enzyme inhibitory activities of green tea and other herbs Megawati, Megawati; Artanti, Nina; Mulyani, Hani; Darmawan, Akhmad; Syahrian, Heri; Lotulung, Puspa Dewi N.; Supriadi, Edi; Widiyarti, Galuh; Dewi, Rizna Triana; Meilawati, Lia; Ernawati, Teni; Dewijanti, Indah Dwiatmi; Minarti, Minarti
Jurnal Gizi Indonesia (The Indonesian Journal of Nutrition) Vol 9, No 1 (2020)
Publisher : Department of Nutrition Science, Faculty of Medicine, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jgi.9.1.48-52

Abstract

Background: Increased lipase activity can increase the amount of monoglycerides and fatty acids absorbed by the body, this is what affects obesity. If pancreatic lipase activity is inhibited, the production of fatty acids will decrease, consequently the level of fat in the blood will also decrease.Objective: To study anti obesity potential of a single herb or a mixture of green tea and other herbs (Syzygium polyanthum. Lv, Artocarpus communis.Lv, Cinnamomum verum..Lv, Manilkara zapota.Lv, Tectona grandis.Lv) by the in vitro inhibition assay of the lipase enzyme activityMethod: A single (8 herbs) or a mixture of three herbs powder (12 formulas) was put into a tea bag with a total weight of 1.5 g to be brewed in 100 mL bottled mineral water at 70⁰-90⁰C for 10 minutes. In vitro lipase enzyme inhibition assay were conducted to measure the ability of these samples as lipase inhibitor. Orlistat used as positive lipase inhibitor. Results: There was a difference results of lipase inhibition activity between the sample of a single and a mixture of herbs. Interestingly, the results shows that formula 3 (mixture of green tea, Manilkara zapota and cinnamon). has 53.942 % inhibition, formula 9 (a mixture of green tea, Syzygium polyanthum and cinnamon) has 67.322 % inhibition and formula 12 (a mixture of green tea, Tectona grandis and cinnamon) has 56.612 % inhibition which close to lipase inhibitory activity by standard Orlistat.Conclusion: The mixture of green tea and two other herbs has a lipase inhibitory activity similar to Orlistat, when compared to the single herb. The highest lipase inhibitory activity is found in formula 9.
Lemongrass (Cymbopogon citratus) Ethanolic Extract Exhibited Activities That Inhibit -glucosidase Enzymes and Postprandial Blood Glucose Elevation Gunawan Puteri, Maria Dewi Puspitasari Tirtaningtyas; Tjiptadi, Felicia Melissa; Hendra, Phebe; Santoso, Filiana; Udin, Zalinar; Artanti, Nina; Florence Ignatia, Florence Ignatia
Makara Journal of Science Vol. 24, No. 4
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Lemongrass is a common ingredient in Indonesian traditional herbal medicine that potently inhibits carbohydrate hydrolysis. In this study, the in vitro α-glucosidase inhibitory (AGI) activity of lemongrass was compared with its in vivo activity to retard postprandial blood glucose elevation, and the bioactive compounds responsible for these activities were observed. Both water and ethanolic extracts of lemongrass (WLG and ELG, respectively) were tested in vitro for its inhibition of the sucrose and maltose hydrolyzing activities of rat intestinal glucosidase. ELG was observed to exert higher inhibitory activities (Sucrase IC50 = 8.74 mg/mL; Maltase IC50 = 18.93 mg/mL) than WLG. ELG was evaluated for its in vivo activity to retard blood glucose elevation in mice after sucrose, maltose, and glucose consumption. ELG was also fractionated using activity-guided chromatography, followed by liquid chromatography–mass spectrometry and high-performance liquid chromatography (HPLC). In vivo sugar tolerance test confirmed the AGI activity in a non-dose-dependent manner and showed potential additional mechanisms that may prevent postprandial hyperglycemia. The active principles were acquired in methanol-soluble fraction and purified using preparative thin-layer chromatography. HPLC analysis with commercial standards identified caffeic acid and kaempferol as the compounds responsible for the bioactivity of ELG. Results showed that lemongrass has the potential as herbal medicine ingredient in the management of diabetes.
Co-Authors A.A. Ketut Agung Cahyawan W Agustine Susilowati Akhmad Darmawan Andini Sundowo Aspiyanto Aspiyanto Dewijanti, Indah Dwiatmi Filiana Santoso Florence Ignatia, Florence Ignatia Galuh Widiyarti Gunawan Puteri, Maria Dewi Puspitasari Tirtaningtyas Hani Mulyani, Hani Heri Syahrian, Heri Ira Djajanegara Jamilah Abbas Ken Ichi Fujita, Ken Ichi Lia Meilawati Linar Zalinar Udin, Linar Zalinar Lotulung, Puspa Dewi N. Lotulung, Puspa Dewi Narij Maryani, Faiza Mcfarlane, Ian James Megawati - Minarti Minarti Pambudi, Sabar Phebe Hendra, Phebe Pratiwi, Rosa Dewi Puji Budi Setia Asih, Puji Budi Puspa D Lotulung, Puspa D Puspa Dewi Narrij Lotulung Puspa Lotulung, Puspa Rina Andriyani Rina Hidayati Pratiwi Rizna Dewi Rizna Triana Dewi Saraswati, Vienna Sofna D.S. Banjarnahor Supriadi, Edi Syafrudin, Din Teni Ernawati Tjiptadi, Felicia Melissa Tutik Murniasih Vienna Saraswaty, Vienna Yati Maryati Yoshinosuke Usuki, Yoshinosuke Zalinar Udin