Article Per Year (5 Year)
p-Index From 2019 - 2024
1.94
P-Index
This Author published in this journals
All Journal AGRISAINS JFMR (Journal of Fisheries and Marine Research) Jurnal Riset Akuakultur Arwana: Jurnal Ilmiah Program Studi Perairan JAGO TOLIS : Jurnal Agrokompleks Tolis Jurnal Ilmiah AgriSains JURNAL SAINS dan INOVASI PERIKANAN Tadulako Science and Technology Journal
Akbar Marzuki Tahya
Akuakultur, Fakultas Peternakan Dan Perikanan, Universitas Tadulako, Palu, Indonesia.
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Control & Systems Engineering Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Engineering Environmental Science Languange, Linguistic, Communication & Media Medicine & Pharmacology Other

Published : 11 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 2 Documents
Search
Journal : AGRISAINS

 1 
Evaluation of CpG DNA for Increasing Immune Gene Expression in Grouper (Epinephelus fuscoguttatus) Jamaluddin, Ruqayyah; Yusuf, Sunarti; Nursida, Nur Fajriani; Suryahman, Agus; Tahya, Akbar Marzuki
AgriSains Vol 21, No 1 (2020)
Publisher : FAPETKAN UNTAD

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (17.935 KB)

Abstract

CpG DNA is a kind of DNA synthetic immunostimulant containing CpG motifs consisting of short sequences containing one or more CpG motifs which act as a danger signal to the natural and innate immune systems. This study aimed to analyze the ability of CpG DNA to increase Interleukin 1β (IL1β) gene expression in tiger grouper so that it could be used as immunostimulant. Analysis of the expression level was carried out by extracting RNA- from the cephalic kidney which was treated with CpG DNA or with PBS as a control. Complementary DNA (cDNA) synthesis was carried out using the Ready To Go You Prime First Strand Beads (GE Healthcare) kit using the RT-PCR technique. The PCR product was analized by measuring the photostimulated luminescence value using UN SCAN IT software based on the thickness of the DNA fragments. The gene expression level and photostimulated luminescence value of the IL1β gene were higher than that of the PBS treatment used as a control. The luminescence value of the photostimulated IL1β gene in CpG DNA treatment was 113684 while the PBS treatment as a control was only 95610.
Phylogenetic Analysis of Mud Crab Scylla olivacea with Several Crustacean Based mRNA Encoding FAMeT Yusuf, Sunarti; Tahya, Akbar Marzuki; Jamaluddin, Ruqayyah
AgriSains Vol 21, No 1 (2020)
Publisher : FAPETKAN UNTAD

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (17.935 KB)

Abstract

Previous studies have succeeded in isolating, and the basic sequence of mRNA encoding FAMeT Scylla olivacea. Several studies have noted that the FAMeT encoding mRNA sequence has differences in crustacean species. The aim of this study was to determine the relationship between S. olivacea and other crustaceans based on the FAMeT encoding mRNA sequence. The similarity of the FAMeT encoding mRNA sequences showed that several crustaceans had similarities to the FAMeT mRNA sequences of S. olivacea. The phylogeny tree analysis showed the existence of 2 clusters of the relationship between the mRNA encoding FAMeT of S. olivacea on the same branch as 2 other crab species: S. serrata and S. paramamosain. It was also found in Portunus trituberculatus and P. pelagicus and river prawn Macrobrachium rosenbergii. These findings indicated that the S. olivacea crab was monophyletic with 2 other mangrove crabs, namely the crab and the river prawn M. rosenbergii. So that describes a very close kinship relationship genetically. Meanwhile, the second branch consists of crustaceans such as Homarus americanus, Cherax quadricarinatus, Fenneropenaeus merguiensis, Metapenaeus ensis and Litopenaeus vannamei.
Co-Authors Andi Dyna Riana Anisa Rizky Putri Aswad Eka Putra Desiana Trisnawati Tobigo Hikmah Asdin Kasim Mansyur Kasim Mansyur, Kasim Madinawati Maspa Timumun Muh. Saleh Nurdin Muhammad Safir Muhammad Safir Nasmia Nopita Novalina Serdiati Novalina Serdiati Nur Fajriani Nursida Nur Hasanah Nursida, Nur Fajriani Ruqayyah Djamaluddin Ruqayyah Jamaluddin Salim Salim Septina F Mangitung Sitti Ramlah Yusuf Sunarti Sunarti Sunarti Sunarti Sunarti Yusuf Suryahman, Agus Teuku Fadlon Haser Yusuf, Sunarti