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All Journal Media Gizi dan Keluarga Jurnal Pendidikan Karakter Dinamika Akuntansi Keuangan dan Perbankan KUMARA CENDEKIA Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Jurnal Ilmu Kesehatan Duta Gama Klaten Majalah Kulit, Karet, dan Plastik Jurnal Hukum IUS QUIA IUSTUM JUSS (Jurnal Sosial Soedirman) Journal of Child Development Studies Jurnal Keperawatan Padjadjaran Jurnal AUDI Economac: Jurnal Ilmiah Ilmu Ekonomi Jurnal Keperawatan Komprehensif Journal of Nursing Care EKOLOGIA : Jurnal Ilmiah Ilmu Dasar dan Lingkungan Hidup Social, Humanities, and Educational Studies (SHEs): Conference Series Open Access Indonesia Journal of Social Sciences FORTE JOURNAL Studium: Jurnal Pengabdian Kepada Masyarakat Jurnal Paradigma Madani Jurnal Pusat Inovasi Masyarakat (JPIM) Relevance: Journal of Management and Business JECIE (Journal of Early Childhood and Inclusive Education) Policy Brief Pertanian, Kelautan, dan Biosains Tropika
DWI HASTUTI
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Journal : Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati

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Pertumbuhan dan Variasi Jumlah Kromosom Akar Rambut Morus macroura Miq. Hasil Transformasi dengan Beberapa Galur Agrobacterium Tri Muji Ermayanti; Dwi Hastuti
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 14, No 2 (2009): June 2009
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v14i2.2687

Abstract

Morus macroura Miq. (Moraceae) which is native West Sumatra is now classified as endangered species and usually used as furniture. This plant produces phenolic compounds. Generally secondary metabolites produced by plants are found in low level, therefore, in vitro techniques such as callus culture, cell suspension and organ cultures are the alternative methods to increase their in vitro production. However, problems on genetic instability such as variation in chromosome numbers and abnormality of chromosome structure are found. These cases influence the productions of secondary metabolites. Genetic variation could be overcome using hairy root culture. The aim of the research was to analyze the growth and chromosome numbers of Morus macroura Miq. hairy root transformed with Agrobacterium strains TISTR-511, TISTR-512, ATCC-15834 and R-1000 in order to determine the genetic variation of the culture. Growth was determined by measuring the fresh and dry weights of roots after 4 weeks in culture. Chromosome numbers was prepared by squashing. The results showed that transformed roots had higher growth compared to the growth of untransformed roots. The growth of hairy roots was varied depending on the strains of Agrobacterium. Both transformed and untransformed roots had high variation in the chromosome numbers. Genetic stability of both transformed and untransformed roots were low but they had similar pattern of distribution on the chromosome number. The roots had the diploid number of chromosome 2n=2x=28 ranged from 35.83 to 46.13% and the tetraploid numbers 2n=4x=56 ranged from 23.23 to 42.21%. Total cells examined were more that 230 cells.
Co-Authors . Hartoyo Abdullah Osman Akbar Kurniawan Alfiasari Aroma Elmina Martha Aulia Rahma, Fadilla M. Bekti Amalia Faron BUDI SETIAWAN Chatarina Suryaningsih Dian Nur Afriyani Dodik Briawan Dwi Kurniati Putri Emilia Emilia Ety Riyani Fauziah Rudhiati Fifi Fauziah Fifi Siti Fauziah Fitri Wulandari Fuadah Ashri Nurfurqoni Hadi Mulyono Hadiyah Hadiyah Happy Primariasari Henik Puji Rahayu Heriberta Heriberta Herien Puspitawati Herien Puspitawati Ibrahim Bola Idris Mohd Noor Ikeu Tanziha Irni Rahmayani Johan Juhriyansyah Dalle Juju Juhaeriah Kartika Andi Laksmi Indiati Leni Novita Lilik N Yuliati Lilik Noor Yuliati Meli Andriyani Melly Latifah Mohammad Nur Indro Muhammad Ridwansyah Novi Indriyani Parmadi Rachmawati Hadori Ratna Megawangi Retna Ningsih Rety Puspitasari Rika Hardani Siti Hodijah Siti Nurbayanti Sri Wulandari Novianti Syaparuddin Syaparuddin Titik Purwati Widowati Titik Sumarti Titik Sumarti Tri Muji Ermayanti Triana Setyawardani Veriati Siregar Yulina Eva Riany Yulmardi Yulmardi Zulkarnain Lubis