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Senyawa Penciri Ekstrak Daun Jati Belanda (Guazuma ulmifolia Lamk) sebagai Anti-Kolesterol Irmanida Batubara; . Husnawati; Latifah Kosim Darusman; Tohru Mitsunaga
Jurnal Ilmu Pertanian Indonesia Vol. 22 No. 2 (2017): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (277.675 KB) | DOI: 10.18343/jipi.22.2.87

Abstract

Jati belanda (Guazuma ulmifolia Lamk) leaves is traditionally used as slimming and cholesterol reducing agent. This research aims to determine the active component on jati belanda leaves as marker compound for cholesterol reducing agent. The quality of leaves was checked prior to use for next step of analysis. Jati belanda leaves with good quality was extracted by ethanol 30% and the extract was separated by chromatography technique such as open column chromatography and preparative high performance liquid chromatography. The results showed that the quality of samples is meet to the requirement of Indonesian Food and Drug Agency (Badan Pengawas Obat dan Makanan (BPOM)) based on moisture content, ash content, and heavy metal content. Based on the separation results showed that the cholesterol reducing compound from jati belanda is quercetin
AKTIVITAS SENYAWA ANTIBAKTERI EKSTRAK PAREPAT (Sonneratia alba) TERHADAP PERTUMBUHAN Ralstonia solanacearum DAN Streptococcus sobrinus Saat Egra; Mardhiana Mardhiana; Ningrum Indah Rahayu; Nurjannah Nurjannah; Sudirman Sirait; Dwi Santoso; Ankardiansyah Pandu Pradana; Harlinda Kuspradini; Tohru Mitsunaga
J-PEN Borneo : Jurnal Ilmu Pertanian Vol 3, No 2 (2020)
Publisher : FAKULTAS PERTANIAN UNIVERSITAS BORNEO TARAKAN

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35334/jpen.v3i2.1624

Abstract

AbstractParepat/pidada putih (Sonneratia alba) is a type of mangrove plant that is used by the traditional tribe for natural medicine. This study uses leaves and stem bark extracted with ethanol. Antibacterial assay uses Ralstonia Solanaceaerum and Streptococcus sobrinus by diffusion agar method. The concentrations used were 5000ppm, 10000ppm, 20000ppm with positive control (Chloramphenicol), and negative control (Ethanol 40%). The results of this study obtain the moisture factor of S. alba leaves has a humidity of 0.31 and stem bark of 0.49. The yield showed that the amount of S. alba leaf extract was 23.86% and the bark was 7.31%. S. alba leaf extract was able to inhibit the bacteria R. solanacearum at concentrations of 5000ppm, 10000ppm and 20000ppm with inhibitory values of 27.46%, 34.34% and 37.78%, respectively. While bark extract can inhibit R. solanacearum at concentrations of 5000 ppm, 10000 ppm and 20000 ppm with inhibitory values of 35.38%, 38.47% and 41.92%, respectively. S. alba leaf extract is able to inhibit S. sobrinus bacteria only at concentrations of 10000 ppm and 20000 ppm with inhibitory values of 28.07% and 48.51%. Whereas S. alba bark extract was able to inhibit S. sobrinus at a concentration of 5000 ppm, 10000 ppm and 20000 ppm with inhibitory values of 16.18%, 49.02% and 61.27%. Keywords: Antibacterial, Leave, Stem bark, Mangrove, S. alba AbstrakParepat/pidada putih (Sonneratia alba) merupakan salah satu jenis tumbuhan mangrove yang dimanfaatkan oleh masyarakat sebagai obat tradisional. Penelitian ini menggunakan daun dan kulit batang yang diekstraksi dengan etanol. Pengujian antibakteri menggunakan Ralstonia Solanaceaerum dan Streptococcus sobrinus dengan metode difusi agar sumuran. Kosentrasi yang digunakan yaitu 5000 ppm, 10000 ppm, 20000 ppm dengan kontrol positif (Chloramphenicol), dan kontrol negatif (Etanol 40%). Hasil penelitian ini menunjukkan faktor kelembaban daun S. alba memiliki kelembaban yaitu 0,31 dan kulit batang yaitu 0,49. Rendemen menunjukkan bahwa jumlah ekstrak daun S. alba yaitu 23,86% dan kulit batang 7,31%. Ekstrak daun S. alba mampu menghambat bakteri R. solanacearum pada konsentrasi 5000ppm, 10000ppm dan 20000ppm dengan nilai persentase hambat yaitu masing-masing 27,46%, 34,34% dan 37,78%. Sedangkan ekstrak kulit batang mampu menghambat R. solanacearum pada konsentrasi 5000 ppm, 10000 ppm dan 20000 ppm dengan nilai persentase hambat secara berturut 35,38%, 38,47% dan 41,92%. Ekstrak daun S. alba mampu menghambat bakteri S. sobrinus hanya pada konsentrasi 10000 ppm dan 20000 ppm dengan nilai persentase hambat yaitu 28,07% dan 48,51%. Sedangkan ekstrak kulit batang S. alba mampu menghambat S. sobrinus pada konsentrasi 5000 ppm, 10000 ppm dan 20000 ppm dengan nilai persentase hambat yaitu 16,18%, 49,02% dan 61,27%. Kata kunci: Antibakteri, Daun, Kulit bantang, Mangrove, S. alba.