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Journal : Medical Laboratory Journal

Uji Efektivitas Antibakteri Dengan Perbedaan Konsentrasi Ekstrak Tinta Cumi-Cumi (Loligo Sp) dan Ekstrak Rumput Laut (Eucheuma cottonii) Pada Perairan Desa Buano Terhadap Bakteri Streptococcus mutans Penyebab Karies Gigi Lukman La Basy; Epi Dusra; Muhammad Azril Hardiman Mahulauw; Sahrir Sillehu; Siti Rahmawaty Tombalissa
Calory Journal : Medical Laboratory Journal Vol. 1 No. 4 (2023): December : Calory Journal : Medical Laboratory Journal
Publisher : LPPM STIKES KESETIAKAWANAN SOSIAL INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.57213/caloryjournal.v1i4.47

Abstract

Squid ink and seaweed have the potential to be utilized as medicines, one of which is as an antibacterial medicinal material. because it contains secondary metabolite compounds that can inhibit the growth of Streptococcus Mutans bacteria. This type of research is a laboratory experiment with the method used, namely maceration for phytochemical screening tests and agar diffusion for antibacterial effectiveness tests with concentrations used in squid ink (Loligo sp) namely 5%, 10%, 15% 20% and in seaweed (Eucheuma cottonii) 1%, 5%, 10%, 15%. The results of the research conducted on phytochemical screening tests contained secondary metabolite compounds in squid ink (Loligo sp) triterpenoids, steroids and seaweed (Eucheuma cottonii) alkaloids, flavonoids, hodroquinone phenols, and tannins. From the research conducted on the antibacterial effectiveness test of squid ink (Loligo sp) and seaweed (Eucheuma cottonii) extracts against Streptococcus mutans bacteria, namely in both samples have the effectiveness in inhibiting Streptococcus mutans bacteria, with a high inhibition of squid ink concentration of 20% by 20.5 mm, and in seaweed concentration of 15% by 23 mm. The analysis used is spss using two tests, namely the shapiro wilk test and the mann whitney test. The conclusion of the mann-whitney test results with a p-value = 0.663 indicates there is no significant difference between squid ink extract (Loligo sp) and seaweed extract (Eucheuma cottonii).
Analisis Perbandingan Ekstrak Daun Jambu Biji (Psidium guajava L.) Terhadap Pertumbuhan Bakteri Propionibacterium acnes dan Staphylococcus aureus Dengan Menggunakan Metode Sumuran Risman Tunny; Epi Dusra; Annisatul Khoiriyah Kaplale; Jayanti Djarami; Maritje.S.J. Malisngorar
Calory Journal : Medical Laboratory Journal Vol. 1 No. 4 (2023): December : Calory Journal : Medical Laboratory Journal
Publisher : LPPM STIKES KESETIAKAWANAN SOSIAL INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.57213/caloryjournal.v1i4.48

Abstract

Acne is a disease on the surface of the skin of the face, neck, chest, and back that appears when the oil glands in the skin are too active so that the skin pores will be clogged by excessive fat deposits. Guava leaves contain secondary metabolites, consisting of tannins, alkaloids, flavonoids, saponins. This study aims to identify the content of secondary metabolites in guava leaf extract (Psidium guajava L.), test the antibacterial activity of ethanol extract of guava leaves (Psidium guajava L.) against the growth of Propionibacterium acnes and Staphylococcus aureus bacteria, and analyze the comparison of the effectiveness of guava leaf extract (Psidium guajava L.) against the growth of the two test bacteria. This type of research is laboratory experimental. The methods used for this study: preparation of materials, extraction of samples by maceration method, phytochemical screening test, testing antibacterial activity using the pitting method and analyzing data. The screening test results of guava leaf extract (Psidium guajava L.) showed the presence of alkaloid, flavonoid, tannin and saponin compounds. The results of the inhibition test on guava leaf extract with a high concentration of 115% have the largest diameter of 17.5 mm against Staphylococcus aureus compared to Propionibacterium acnes bacteria with an inhibition diameter of 16 mm. The results of the Mann-Whitney statistical test showed a p-value of 0.077 so it can be concluded that there is no comparison of the effectiveness of guava leaf extract (Psidium guajava L.) against the growth of Propionibacterium acnes and Staphylococcus aures bacteria.