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All Journal Jurnal Veteriner Jurnal NERS Majalah Obstetri dan Ginekologi The Indonesian Biomedical Journal Jurnal Penelitian Kesehatan Indian Journal of Forensic Medicine & Toxicology

Hendy Hendarto

Departemen Obstetri Dan Ginekologi, Fakultas Kedokteran, Universitas Airlangga

Author-ID : 2534368

Biochemistry, Genetics & Molecular Biology Health Professions Medicine & Pharmacology Nursing Public Health Veterinary

Published : 13 Documents Claim Missing Document

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Kurkumin Menurunkan Ekspresi Tumor Necrosis Factor (TNF)-α Kompleks Oosit-Kumulus Sapi pada Kultur dengan Zalir Peritoneum Penderita Infertil Terkait Endometriosis MY Ardianta W; Hendy Hendarto; Widjiati Widjiati
Majalah Obstetri dan Ginekologi Vol. 23 No. 3 (2015): September - Desember 2015
Publisher : Universitas Airlangga

Tujuan: Mempelajari pengaruh pemberian kurkumin terhadap ekspresi tumor necrosis factor (TNF)-α kompleks oosit-kumulus (KOK) sapi pada kultur zalir peritoneum penderita infertil dengan endometriosis ringan dan berat.Bahan dan Metode: Penelitian ini merupakan penelitian eksperimental murni yang dilaksanakan di Laboratorium Embrio-logi Fakultas Kedokteran Hewan Universitas Airlangga antara April sampai Agustus 2014. Zalir peritoneum diambil dari penderita infertil dengan endometriosis ringan, endometriosis berat, dan non-endometriosis yang menjalani laparoskopi diagnosis di Klinik Fertilitas Graha Amerta RSUD dr. Sutomo Surabaya, RS Bersalin Putri Surabaya, RSIA Kendangsari Surabaya, RS Universitas Airlangga Surabaya. Masing-masing zalir peritoneum endometriosis ringan dan berat dibagi lagi menjadi zalir peritoneum endometriosis dengan kurkumin dan tanpa kurkumin. Zalir peritoneum non endometriosis dikelompok-kan menjadi zalir peritoneum penderita non endometriosis tanpa dan dengan kurkumin. KOK sapi diambil secara consecutive sampling dari aspirasi folikel antral diameter 3–8 mm dari ovarium sapi yang berasal dari Rumah Potong Hewan Surabaya kemudian secara acak dengan randomisasi sederhana dikultur dalam tujuh kelompok. Kelompok 1 ditempatkan dalam tissue culture medium 199 (TCM-199) saja. Kelompok 2, 3 dan 4 TCM-199 ditambah masing-masing 3% zalir peritoneum penderita infertil dengan endometriosis ringan, berat dan non-endometriosis. Kelompok 5, 6, dan 7 media TCM-199 ditambah masing-masing 3% zalir peritoneum penderita infertil dengan endometriosis ringan, berat dan non-endometriosis dan dengan kurkumin 20 µg/ml.Hasil: Uji Kruskal Wallis menunjukkan perbedaan yang bermakna ekspresi TNF-α pada ketujuh kelompok (p<0,0001). Uji Mann-Whitney juga menunjukkan bahwa ekspresi TNF-α KOK sapi pada kultur kelompok endometriosis ringan dengan kurkumin (ER+C) lebih rendah secara bermakna dibandingkan kelompok endometriosis ringan tanpa kurkumin (ER). Ekspresi TNF-α KOK sapi pada kultur kelompok endometriosis berat dengan kurkumin (EB+C) lebih rendah secara bermakna dibandingkan kelompok endometriosis berat tanpa kurkumin (EB). Uji Mann-Whitney menunjukkan ekspresi TNF-α KOK sapi pada kultur kelompok ER lebih tinggi secara bermakna dibandingkan kontrol ataupun kelompok non endometriosis (NE). Ekspresi TNF-α KOK sapi pada kultur kelompok EB lebih tinggi secara bermakna dibandingkan kontrol ataupun kelompok NE. Ekspresi TNF-α KOK sapi pada kultur kelompok EB tidak didapatkan perbedaan yang bermakna dibandingkan dengan kelompok ER.Simpulan: Pemberian kurkumin dapat dipertimbangkan untuk digunakan sebagai terapi tambahan pada penderita infertil terkait endometriosis.

Stres Infertilitas Menghambat Maturasi Oosit dan Hasil Fertilisasi In Vitro Hendy Hendarto
Majalah Obstetri dan Ginekologi Vol. 23 No. 1 (2015): Januari - April 2015
Publisher : Universitas Airlangga

Tujuan: Melakukan evaluasi hubungan antara stres infertilitas terhadap maturasi oosit dan hasil fertilisasi in vitro.Bahan dan Metode: Penelitian ini adalah studi potong lintang analitik observasional. Dilakukan di klinik Fertilitas Graha Amerta RSUD Dr Soetomo Surabaya. Tercatat 30 subyek penelitian perempuan infertil yang mengikuti fertilisasi in vitro dan telah dilakukan evaluasi tingkat stres dengan menggunakan Infertility Reaction Scale. Maturasi oosit dan hasil fertilisasi dicatat. Hubungan antara stres infertilitas, maurasi oosit dan hasil fertilisasi dihitung dengan Anova.Hasil: Semua subyek penelitian yang mengikuti fertilisasi in vitro mengalami stres infetilitas dari kadar ringan sampai berat. Pada kelompok tingkat stres infertilitas berat didapatkan oosit matur lebih sedikit (p=0.01). Pada kelompok tingkat stres infertilitas ringan didapatkan oosit matur lebih banyak (p=0.00). Tingkat stres infertilitas berat akan menghasilkan fertilisasi lebih sedikit (p=0.00)Simpulan: Semakin berat stres infertilitas akan menghambat maturasi oosit dan hasil fertilisasi in vitro.

M1 M2 macrophage expression in menstrual blood flakes of women with endometriosis Yulisa Haslinda; Ashon Sa’adi; Hendy Hendarto; Faroek Hoesin
Majalah Obstetri dan Ginekologi Vol. 24 No. 2 (2016): May - August
Publisher : Universitas Airlangga

Objectives: to measure and prove the increase of panmacrophage, macrophages M1 and M2 expression and decrease of ratio of M1/M2 in menstrual blood flakes of women with endometriosis. Materials and Methods: This study was a cross sectional observational analytic study conducted on 30 subjects with endometriosis and non-endometriosis. Immunohistochemical staining was done on a sample of menstrual blood flakes of subjects study who were taken at the second or third day of menstrual cycles with CD68 and CD163 antibody to measure the expression of panmacrophage and M2 macrophages. Expression of M1 macrophages is the approach of a reduction expression of panmacrophage with M2 macrophages.Results: Expression of M1, M2 and the ratio M1/M2 in the both of groups had a normal distribution then continued by independent t-test with one-tailed α (0.05). Probability was considered statistically significant at p <0.05 with a confidence interval of 95%. Based on the statistical result, Mφ macrophage expression in endometriosis and control group amounted to 3.62 ± 0.50 and 2.80 ± 0.64 (p =0.0005) with non parametric test. The expression of M1 macrophages in endometriosis group and non endometriosis were respectively 1.40 ± 0.35 and 1.33 ± 0.40 (p =0.3005) and the expression of M2 in both of group, respectively of 2.23 ± 0.41 and 1.47 ± 0.36 (p =0.0005). The ratio of M1/M2, the endometriosis group and non endometriosis, respectively of 0.65 ± 0.20 and 0.92 ± 0.24 (p =0.0015).Conclusion: this study were significant increased in the panmacrophage Mφ, M2 macrophages expression on a woman's menstrual blood flakes endometriosis and significant decreased in ratio M1/M2 in the woman's menstrual blood flakes endometriosis.

Different expression of NF-kB and endometrial implant width in the administration of red fruit (Pandanus conoideus Lam) and leuprolide Raudatul Hikmah; Hendy Hendarto; Widjiati Widjiati
Majalah Obstetri dan Ginekologi Vol. 30 No. 1 (2022): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mog.V30I12022.1-9

HIGHLIGHTS 1. Endometriosis treatment remains controversial, whether it should be managed with hormonal modulation and/or surgical removal of the lesion.2. NF-kB becomes potential therapeutic target as it has constitutive activation in peritoneal endometriosis.3. It has been recognized that NF-kB expression decreases when red fruit extract is given.4. Effect of red fruit extract, compared to leuprolide, a widely used treatment for endometriosis, on NF-kB expression was examined.5. NF-kB was significantly lower in endometriotic group receiving red fruit extract than in group receiving leuprolide. ABSTRACT Objectives: This study aimed to prove the effect of different administration of red fruit (Pandanus conoideus Lam) extract and leuprolide on the expression of NF-kB and endometrial implant width in mouse models of endometriosis. Materials and Methods: This study used completely randomized design with pre-post separated sample. The sample size was 36 female mice (Mus muculus), which were divided into 3 groups randomly. They were given with cyclosporine A, estrogen and endometrial tissue to form endometriosis model. Results: The mean expression of NF-kBin in treatment group receiving leuprolide (4.13 + 0.70) and red fruit (2.70 + 1.08) were significantly lower than that in control group (6.94 + 1.007). Whereas, in treatment group receiving red fruit (2.70 + 1.08), it was significantly lower than in those receiving leuprolide (4.13 + 0.70). Mean endometrial implant width in treatment group receiving leuprolide (13.62 + 3.21) and red fruit (8.93 + 2.74) were significantly lower than that in control group (28.89 + 8.28). Whereas, in treatment group receiving leuprolide (13.62 + 3.21), it was not significantly higher than in those receiving red fruit (8.93 + 2.74). Conclusion: The expression of NF-kB was significantly lower after the administration of red fruit than leuprolide. The width of endometrial implant was not significantly lower after the administration of red fruit than after the administration of leuprolide.

Co-Authors Anak Agung Renjani Kanya Kumari Ashon Sa’adi Dian Irawati Faroek Hoesin Hening Laswati I'tishom, Reny Juminten Saimin Laba Mahaputra MY Ardianta W Nur Anindya Syamsudi Raudatul Hikmah Soetjipto Soetjipto Sri Agus Sudjarwo Sri Ratna Dwiningsih Syarifah, Anis Satus Tinta Julianawati Widjiati w Widjiati Widjiati Widjiati Widjiati Widjiati Widjiati Yulisa Haslinda

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