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All Journal Jurnal Hortikultura Indonesia Jurnal Ilmu Tanah dan Lingkungan (Journal of Soil Science and Environment) Journal of Tropical Soils Jurnal Hortikultura Jurnal Penelitian Sains Agroekoteknologi Tropika Lembab Business Review and Case Studies Jurnal Sains Terapan : Wahana Informasi dan Alih Teknologi Pertanian Prosiding Seminar Nasional Biologi

Fahrizal Hazra

Departemen Ilmu Tanah Dan Sumberdaya Lahan, Fakultas Pertanian, Institut Pertanian Bogor, Jl. Meranti Kampus IPB Dramaga, Bogor 16680

Author-ID : 2549016

Agriculture, Biological Sciences & Forestry Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Environmental Science Mathematics Physics Social Sciences Veterinary

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Exploration of Pectin – Utilizing Yeast From Soil of Bogor and Wleri Fruit Orchards Fahrizal Hazra
Jurnal Hortikultura Indonesia Vol. 2 No. 1 (2011): Jurnal Hortikultura Indonesia
Publisher : Indonesian Society for Horticulture / Department of Agronomy and Horticulture

There is a high demand on pectin–utilizing yeasts for industrial, agricultural and environmental purposes. Further exploration of yeast from various sources are important to enrich yeast culture collections. Nine yeast strains were isolated from various soil sources sampled based on biological sampling in Bogor and Central Java. Enriched media containing pectin as carbon sources was employed for isolation of the yeast. The isolated yeast were identified according to the methods described in monographs by Kreger – Van Rij (1984), Barnett et.al (2000), Guilliermond and Tanner (2006) . The strains isolated were taxonomically separated into 3 groups. Group I contains 3 strains, and this group is closely related to Candida tropicalis. Group II contains 4 strains, and this group is included in this genus Rhodotorula. Group III contain 2 strains, and this group is closely related to Williopsis saturnus, which is a synonym of Hansenula saturnus. Pectinolytic enzymes (Polygalacturonase) were produced by all of the tested strains. Polygalacturonase was produced as high as 1.7 U.ml-1 by strain no. 111 of group I, 1.7 U.ml-1 by strains no. 123 of group II, and 1.0 U.ml-1 by strain no. 211 of group III.Key words : yeast, pectin, polygalacturonase

Pertumbuhan Bibit Salak [Salacca zalacca (Gaertner) Voss] Pondoh yang Diinokulasi dengan Isolat Bakteri Potensial di Tanah Regosol Darmaga Fahrizal Hazra
Jurnal Hortikultura Indonesia Vol. 6 No. 1 (2015): Jurnal Hortikultura Indonesia
Publisher : Indonesian Society for Horticulture / Department of Agronomy and Horticulture

ABSTRACTSnake fruit [Salacca zalacca (Gaertner) Voss] Pondoh is one of important fruits in Indonesia. The objective of the study was to determine the influence of some selected potential microbes such as Phosphate Solubilizing Bacteria, Cellulolytic Bacteria, and N2-fixing Bacteria (Azospirillum) ongrowth of seedling of snake fruit Pondoh. A study was conducted in the nursery. The experiment used a completely randomized design with treatments namely: K0 (without inoculum); A1 (Azospirillum inoculum dose 5 ml), A2 (inoculum 25 ml); F1 (BPF inoculum dose 5 ml), F2 (dose 25 ml); S1; BPS inoculums (dose 5 ml), S2 (dose 25 ml); C1 (mixed bacterial inoculum A+F+S dose 5 ml), and C2 (A+F+S dose 25 ml). Plant height, P, C/N soil ratio, N were measured in 10 months for observing the impact of treatments. Results showed that the effect of inoculation of A2 to the amount of N-total (0.99%) was significantly different compared to soil with K0; and not significantly different from the amount of N-total inoculation A1, F1, F2, S1, S2, C1, and C2. F2 increased availability of P (20.79 ppm) in the soil higher than K0, A1, F1; and not significantly different from A2, S1, S2, C1, and C2. K0 treatment gave C/N ratio of soil significantly different from A1, A2, F1, F2, S1, S2, C1 and C2; BPS which showed activity in overhauling C-organic in the soil. Inoculation A2 and F2 increased plant height 15.48% and 9.31% higher than the control. Inoculation of C2 differed significantly in improving the fresh weight compared to control; and not significantly different from A1, A2, F1, F2, S1, S2, and C1 and able to increase the dry weight of 71.12% compared to the control.Keywords: Azospirillum, Cellulolytic Bacteria, Phosphate Solubilizing Bacteria, Snake Fruit ABSTRAKSalak Pondoh [Salacca zalacca (Gaertner) Voss] merupakan tanaman buah bernilai tinggi dan diminati masyarakat. Budidaya salak diawali dengan pembibitan. Penelitian ini bertujuan menguji pengaruh bakteri potensial hasil isolasi dan seleksi dari bakteri pelarut fosfat (BPF), bakteri perombak selulosa (BPS), dan bakteri pemfiksasi N2 (Azospirillum) dalam pertumbuhan bibit tanaman salak pondoh. Penelitian ini menggunakan rancangan acak lengkap satu faktor yang terdiri atas: K0 (tanpa inokulum/Kontrol); A1 (inokulum Azospirillum 5 ml), A2 (25 ml inokulum); F1 (inokulum BPF dosis 5 ml), F2 (dosis 25 ml); S1 (inokulum BPS dosis 5 ml), S2 (dosis 25 ml); C1 (inokulum campuran A+F+S dosis 5 ml), dan C2 (dosis 25 ml). Pengamatan dilakukan selama 10 minggu sampai bibit salak Pondoh memiliki dua daun. Hasil penelitian menunjukkan inokulasi A2dan S2 nyata jumlah N-total (0.99%) di dalam tanah dibandingkan dengan K0; tetapi tidak berbeda nyata dibandingkan dengan perlakuan lain. Inokulasi F2 nyata meningkatkan P tersedia (20.79 ppm) di dalam tanah dibandingkan dengan K0, A1, F1; dan tidak berbeda nyata dengan A2, S1, S2, C1, dan C2. Induksi dengan seluruh isolat nyata terhadap C/N rasio tanah; yang menunjukkan adanya aktivitas BPS dalam merombak C-organik di dalam tanah. Inokulasi A2 dan F2 mampu meningkatkan tinggi tanaman 9.31% dan 15.48% lebih besar dibandingkan kontrol. Inokulasi C2 nyata meningkatkan bobot basah bibit salak pondoh dibandingkan kontrol; tetapi tidak berbeda nyata dengan perlakuan lain serta mampu meningkatkan bobot kering 71.12% dibandingkan kontrol.Kata kunci: Azospirillum, bakteri pelarut fosfat, bakteri perombak selulosa, Salak Pondoh

Co-Authors Aisamrotul Hasanah Ania Citraresmini Ans Iswandi Dedi Nursyamsi Dedi Nursyamsi Nursyamsi Devi Wijayanti Dian Emilia Maulidiyah Dimas Syahiddin DWI ANDREAS SANTOSA Enny Widyati Eric Verchius Ester LN Lumbantobing Fatimah Nur Istiqomah Grahan Sugeng Aprilian Gusmaini Gusmaini Heni Hariyani Intan Delia Rustandi Irfan Septiawan Iswandi Anas Lisa Rosdiana LISDAR I. SUDIRMAN Lusiana Adriani Mentari May Syachanna Muhammad Abdul Aziz Muhammad Abdul Aziz Muhammad Samsul Maarif Nabila Dianisa Novita Sari RAHAYU WIDYASTUTI Richard Gunawan Rifqi Puja Novtiar Rio Kevin Marcello Alwi Risa Rosita Rosinta Sitepu Selly Salma Selly Salma Sindy Marieta Putri Sukmadewi, Desak Ketut Tristiana Suri Mulyani Sari Susanti Pratiwi Purnama Velicia Desyana Rakhmadina Wilhelmus Terang Arga Sanjaya Windi Windi Yunilda Rosa Yuwan Pratama Baki

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