Article Per Year (5 Year)
p-Index From 2019 - 2024
1.116
P-Index
This Author published in this journals
All Journal Majalah Obstetri dan Ginekologi Jurnal Surya Medika Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML)
Ashon Sa’adi
Department Of Obstetrics And Gynecology, Faculty Of Medicine, Airlangga University/Dr. Soetomo Hospital, Surabaya
Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Public Health

Published : 7 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 2 Documents
Search
Journal : Jurnal Surya Medika

 1 
Analisis Antimullerian Hormon (AMH) di Dalam Serum pada Berbagai Kategori Indeks Masa Tubuh Didik Rio Pambudi; Ashon Sa’adi; Sudjarwo Sudjarwo
Jurnal Surya Medika (JSM) Vol 5 No 1 (2019): Jurnal Surya Medika (JSM)
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (500.597 KB) | DOI: 10.33084/jsm.v5i1.956

Abstract

Obesity-related to the result of decreased reproduction. Obese women are more prone to abnormal anovulation and uterine bleeding, endometrial hyperplasia/cancer, infertility, miscarriage, and pregnancy complications, compared to women of normal weight. This study aims to determine the levels of anti-Mullerian hormone (AMH) in the serum at various BMI (body mass index), also to determine the relationship and correlation between obesity and AMH levels in serum. The population in the study were women aged 20 years to 40 years with less BMI, Normal BMI and Obesity BMI. The study subjects were women between the ages of 20 to 40 years with a BMI less than 17-19.9, obesity BMI ≥ 25 and normal BMI 20-25 as controls. Height measurement, weight weighing, and BMI calculation carried out according to the standard and subject to approval. Taking blood samples for the examination of AMH levels carried out by the RSKI laboratory (Infection Special Hospital) Airlangga University. The results of the data processed with SPSS 25 with the Shapiro-Wilk normality test and Mann Whitney statistical analysis for different tests and Spearman analysis for the correlation test. The results of the study found homogeneous samples, there were no significant differences between the AMH levels of the less and obese groups with, p = 0.832 (p> 0.05). AMH levels in BMI were less (0.459 ± 0.112 ng / mL) than obesity BMI (0.432 ± 0.058 ng / mL), so it was concluded that AMH levels did not correlate with less BMI with obesity BMI, with a correlation value (r) = -0.105 (p = 0.643; p> 0.05). The results of this study concluded that body mass index not related and does not correlate with the levels of anti-Mullerian hormone in the serum.
Verifikasi Metode ELISA (Enzym Linked Immunosorbent Assay) Untuk Penentuan Kadar AMH (Anti Mullerian Hormone) Irvan Ipandi; Ashon Sa'adi; Sudjarwo Sudjarwo
Jurnal Surya Medika (JSM) Vol 5 No 1 (2019): Jurnal Surya Medika (JSM)
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (875.094 KB) | DOI: 10.33084/jsm.v5i1.973

Abstract

Validation is the process of confirming that the method used has capabilities that are consistent with what is needed while being used continuously. The AMH ELISA kit has carried out a method validation process before marketed and used in a laboratory to determine AMH levels. AMH is a specific biomarker used to diagnose PCOS patients. The laboratory can adopt a validated procedure but the laboratory still needs to confirm its ability to apply this method. Verification is the provision of objective evidence that the measured parameters meet the specified requirements. The purpose of this study was to find out how well verification of the ELISA AMH kit and comparing the calculation of 4 PL with linear regression. Based on the statistical test obtained p = 0.871 (p>0.05). It can be said that there was no significant difference between Optical Density days 1 and 7(p = 0.05). Linear regression equation (r) 0.9543 (p = 0.000; p <0.05) while in equation 4 PL (r) 1,000. The ELISA kit AMH method meets the verification requirements. The 4PL model calibration curve is more suitable to be used in the AMH ELISA kit than the linear regression model.
Co-Authors Andarisa Rachman Nugraha Audia Mumtaz Rifasky Berliana Hamidah Didik Rio Pambudi Faizah, Zakiyatul Faroek Hoesin Hamdani Lunardhi Hantoro Gunawan Hendy Hendarto Irvan Ipandi Irvan Ipandi Jusak Nugraha Ni Wajan Tirthaningsih Ninik Darsini Puspa Wardhani Rina Yudiwati Sudjarwo Sudjarwo Yulisa Haslinda