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All Journal Majalah Obstetri dan Ginekologi Jurnal Surya Medika Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML)
Ashon Sa’adi
Department Of Obstetrics And Gynecology, Faculty Of Medicine, Airlangga University/Dr. Soetomo Hospital, Surabaya
Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Public Health

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Journal : Majalah Obstetri dan Ginekologi

 1 
M1 M2 macrophage expression in menstrual blood flakes of women with endometriosis Yulisa Haslinda; Ashon Sa’adi; Hendy Hendarto; Faroek Hoesin
Majalah Obstetri dan Ginekologi Vol. 24 No. 2 (2016): May - August
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (135.007 KB) | DOI: 10.20473/mog.V24I22016.64-69

Abstract

Objectives: to measure and prove the increase of panmacrophage, macrophages M1 and M2 expression and decrease of ratio of M1/M2 in menstrual blood flakes of women with endometriosis. Materials and Methods: This study was a cross sectional observational analytic study conducted on 30 subjects with endometriosis and non-endometriosis. Immunohistochemical staining was done on a sample of menstrual blood flakes of subjects study who were taken at the second or third day of menstrual cycles with CD68 and CD163 antibody to measure the expression of panmacrophage and M2 macrophages. Expression of M1 macrophages is the approach of a reduction expression of panmacrophage with M2 macrophages.Results: Expression of  M1, M2 and the ratio M1/M2 in the both of groups had a normal distribution then continued by independent t-test with one-tailed α (0.05). Probability was considered statistically significant at p <0.05 with a confidence interval of 95%. Based on the statistical result, Mφ macrophage expression in endometriosis and control group amounted to 3.62 ± 0.50 and 2.80 ± 0.64 (p =0.0005) with non parametric test. The expression of M1 macrophages in endometriosis group and non endometriosis were respectively 1.40 ± 0.35 and 1.33 ± 0.40 (p =0.3005) and the expression of M2  in both of group, respectively of 2.23 ± 0.41 and 1.47 ± 0.36 (p =0.0005). The ratio of M1/M2, the endometriosis group and non endometriosis, respectively of 0.65 ± 0.20 and 0.92 ± 0.24 (p =0.0015).Conclusion: this study were significant increased in the panmacrophage Mφ, M2 macrophages expression on a woman's menstrual blood flakes endometriosis and significant decreased in ratio M1/M2 in the woman's menstrual blood flakes endometriosis.
Profile study of ectopic pregnancy at Department of Obstetrics and Gynecology, Dr. Soetomo Hospital, Surabaya, Indonesia Andarisa Rachman Nugraha; Ashon Sa’adi; Ni Wajan Tirthaningsih
Majalah Obstetri dan Ginekologi Vol. 28 No. 2 (2020): August
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mog.V28I22020.75-78

Abstract

Objective: Ectopic pregnancy is a leading cause of maternal death during the first trimester of pregnancy, it can cause infertility and even death as a result of massive bleeding of the mother. Based on those fact, study of ectopic pregnancy is needed.Materials and Methods: This study used retrospective design and total sampling to collect the data and present it as a descriptive – analytical result.Results: Of the 98 samples, as many as 30.6% of the patients age was in the range of 26 - 30 years, there was 16.3% of patients with unmarried status, hormonal contraception was more often used by patients than intrauterine device, there were 7% of patients with recurrent ectopic pregnancy, as many as 12.1% of patients had a history of surgery in the abdomen or pelvic area, ectopic pregnancy was most common in first pregnancies, and there were 26.4% of patient found with Infection.Conclusion: Ectopic pregnancy has many risk factors, Ectopic pregnancy could be cause by solely one risk factor or more (multifactorial), The most commonly found risk factor was infection.
Comparison of pregnancy rates on day 3 and day 5 embryo transfer in In Vitro Fertilization (IVF) Audia Mumtaz Rifasky; Puspa Wardhani; Ashon Sa’adi; Ninik Darsini; Hamdani Lunardhi; Zakiyatul Faizah
Majalah Obstetri dan Ginekologi Vol. 29 No. 1 (2021): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mog.V29I12021.14-17

Abstract

Objectives: To identify the success rates of pregnancy on the third and fifth day embryo transfer at Graha Amerta Hospital, Surabaya, Indonesia.Materials and Methods: This study used comparative cross sectional design. Data were taken from medical record of IVF participants who met the inclusion and exclusion criteria at Graha Amerta Hospital for the period of January 2016 - December 2016.Results: Successful pregnancy rates were found in this research. The embryo transfer on the third day and the fifth day were 35% and 49.3% respectively. In other words, the rates of pregnancy success were not affected by embryo transfer on the third day and the fifth day in the medical record sample as it had p value of 0.090.Conclusion: Embryo transfer on the third and fifth days had the same rates of pregnancy success in IVF participants at Graha Amerta Hospital, Surabaya, Indonesia. 
Co-Authors Andarisa Rachman Nugraha Audia Mumtaz Rifasky Berliana Hamidah Didik Rio Pambudi Faizah, Zakiyatul Faroek Hoesin Hamdani Lunardhi Hantoro Gunawan Hendy Hendarto Irvan Ipandi Irvan Ipandi Jusak Nugraha Ni Wajan Tirthaningsih Ninik Darsini Puspa Wardhani Rina Yudiwati Sudjarwo Sudjarwo Yulisa Haslinda