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Korelasi Antara Hypoxia Inducible Factor-1α Dengan Aktivitas Enzim Creatine Kinase Jaringan Otot Jantung Tikus Pada Aktivitas Fisik Aerobik dan Anaerobik Flora, Rostika
Biomedical Journal of Indonesia: Jurnal Biomedik Fakultas Kedokteran Universitas Sriwijaya Vol 1, No 1 (2015)
Publisher : Fakultas Kedokteran Universitas Sriwijaya

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Abstract

Aktivitas fisik aerobik dan anaerobik tanpa hari istirahat dapat mengakibatkan terjadinya hipoksia dan perubahan histopatologi pada otot jantung. Kondisi hipoksia berkorelasi terhadap perubahan histopatologi pada otot jantung. Untuk mengetahui apakah kondisi hipoksia ini juga berkorelasi dengan indikator kerusakan jaringan otot jantung, perlu dilakukan penelitian lebih lanjut. Penelitian ini bertujuan untuk mengetahui korelasi antara hipoksia yang terjadi pada otot jantung akibat pembebanan aktivitas fisik aerobik dan anerobik tanpa hari istirahat dengan aktivitas enzim CK jaringan otot jantung. Jaringan otot jantung berasal dari tikus yang diberi aktivitas fisik aerobik dan anaerobik menggunakan treadmill selama 1,3,7 dan 10 hari tanpa hari istirahat. Kemudian dilakukan pengukuran kadar HIF-1α sebagai parameter hipoksia dan aktivitas enzim CK sebagai parameter terjadinya kerusakan sel otot jantung. Hasil penelitian menunjukkan bahwa, terdapat korelasi yang sangat kuat  antara konsentrasi HIF-1α dengan aktivitas CK jaringan pada kelompok anaerobik (r=0,91, p<0,05), sedangkan pada kelompok aerobik terdapat korelasi yang sedang (r=0,48, p<0,05). Kondisi hipoksia pada otot jantung yang terjadi akibat aktivitas fisik aerobik dan anaerobik  yang dilakukan selama 10 hari tanpa hari istirahat berkorelasi sangat kuat dengan indikator kerusakan jaringan pada kelompok anaerobik dan berkorelasi sedang pada kelompok aerobik.
Correlation of hypoxia inducible factor-1α and vascular endothelium growth factor in rat myocardium during aerobic and anaerobic exercise Freisleben, Hans-Joachim; Flora, Rostika; Wanandi, Septelia I.; Ferdinal, Frans; Sadikin, Mohamad
Medical Journal of Indonesia Vol 21, No 3 (2012): August
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (837.72 KB) | DOI: 10.13181/mji.v21i3.493

Abstract

Background: Exercise increases the need for oxygen to generate ATP through oxidative phosphorylation. If the high energy demand during exercise is not balanced by sufficient oxygen supply, hypoxia occurs in skeletal muscle tissue leading to upregulation of hypoxia inducible factor-1α (HIF-1α). The activity of HIF-1α increases the expression of various genes in order to reduce the metabolic dependence on oxygen and to increase oxygen supply to the tissue, e.g., VEGF which plays a role in angiogenesis. In myocardium, it is unclear whether exercise leads to hypoxia and whether HIF-1α and VEGF play a role in the mechanism of hypoxic adaptation. This study aimed to investigate the correlation of HIF-1α and VEGF in heart muscle tissue of rats during aerobic and anaerobic exercise.Methods: A rat treadmill was used with a specific exercise program for 1, 3, 7 and 10 days. The concentrations of HIF-1α and VEGF were measured the myocardium.Results: Both, HIF-1α protein and VEGF were increased (p &lt; 0.05) in the groups with aerobic and anaerobic exercise. Concentrations of HIF-1α were highest on the first day of activity, being higher in the anaerobic than in the aerobic group (156.8 ± 33.1 vs. 116.03 ± 5.66). Likewise, the highest concentration of VEGF in the group with anaerobic exercise occurred on the first day (36.37 ± 2:35), while in the aerobic group, VEGF concentration was highest on day 3 (40.66 ± 1.73). The correlation between the myocardial tissue consentrations of HIF-1α and VEGF is moderate (r = 0.59) in the aerobic group and strong in the anaerobic group (r = 0.69).Conclusion: Aerobic and anaerobic exercise increase HIF-1α and VEGF concentrations in rat myocardium in specific patterns. The anaerobic condition triggers vascularization stronger and obviously earlier than aerobic exercise. (Med J Indones. 2012;21:133-40)Keywords: Exercise, HIF-1α, myocardium, VEGF
Myocardial damage after continuous aerobic and anaerobic exercise in rats Freisleben, Hans-Joachim; Sadikin, Mohamad; Wanandi, Septelia I.; Hernowo, Bethy S.; Ferdinal, Frans; Flora, Rostika
Medical Journal of Indonesia Vol 22, No 4 (2013): November
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (813.04 KB) | DOI: 10.13181/mji.v22i4.601

Abstract

Background: Regular physical activity is highly recommended in preventive, curative, and rehabilitative programs in order to promote health, especially cardiovascular health. However, physical activity can also cause sudden death. In athletes, sudden death may occur during sport competitions, with myocardial infarction as the most common etiology. It is suspected that continuous training without any rest-day play a role in cardiac muscle damage and sudden death during competition. Our study was aimed to learn about cardiac muscle adaptation on continuous aerobic and anaerobic physical activity without any rest-day. Methods: The specimens in our study were cardiac muscle tissue obtained from rats that had performed aerobic and anaerobic physical activity on treadmill for 1, 3, 7, and 10 days without any rest-day. Blood gas analysis and hematological assessment were used as parameters of systemic adaptation to hypoxia during physical activity. Moreover, histopathology of cardiac muscle tissue was performed as parameter for cardiac muscle damage.Results: The results showed that aerobic and anaerobic physical activity caused a systemic hypoxic condition and triggered adaptation responses. Cardiac muscle damage occurred on the 10th day in both treatment groups, with more severe damage observed in the group with anaerobic physical activity. The tissue protein level in the anaerobic group increased progressively on the 10th day.Conclusion: Physical activity may result in hypoxia and systemic adaptation. Aerobic and anaerobic physical activities performed for 10 days without any rest-day may cause cardiac muscle damage. (Med J Indones. 2013;22:209-14. doi: 10.13181/mji.v22i4.601)Keywords: Cardiac muscle, cardiac muscle damage, histopathology, physical activity
Chronic physical exercise increases a neurogenesis marker within hippocampus Andrianti, Septi; Flora, Rostika; Zulkarnain, Mohammad
Medical Journal of Indonesia Vol 27, No 2 (2018): June
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (349.964 KB) | DOI: 10.13181/mji.v27i2.1685

Abstract

Background:Aerobic and anaerobic physical exercises conducted in both acute and chronic are really essential in keeping the body especially brain healthy. Physical exercise plays an important role in molecular system and is beneficial for the brain by enhancing neurogenesis which is mediated by the increase of BDNF level. This study aimed to evaluate the effect of physical exercise to the BDNF level of hippocampus tissues in Wistar rats.Methods: Thirty male rats were divided into five groups i.e. control group, acute aerobic physical exercise group, acute anaerobic physical exercise group, chronic aerobic physical exercise group, and chronic anaerobic physical exercise group. Physical exercises were conducted on animal treadmill. The level of hippocampus BDNF was determined using ELISA. The data were analyzed using independent t-test.Results: BDNF average levels of chronic aerobic and anaerobic physical exercises were higher than those of acute ones (152.86±1.62 pg/ml and 122.22±1.53 pg/ml vs 59.38±6.10 pg/ml and 54.05±3.35 pg/ml). There were significant differences in the BDNF average levels of hippocampus tissues between aerobic and anaerobic groups, in both acute and chronic exercise.Conclusion: The chronic physical exercises, both aerobic or anaerobic, are increasing higher the level of BDNF in brain tissue.
Indikator Beban Berlebih Pada Otot Jantung Tikus Wistar Yang Diberi Perlakuan Latihan Fisik Akut Theodorus, Theodorus; Flora, Rostika; Rahima, Rahima
Biomedical Journal of Indonesia: Jurnal Biomedik Fakultas Kedokteran Universitas Sriwijaya Vol 1, No 1 (2015)
Publisher : Fakultas Kedokteran Universitas Sriwijaya

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Abstract

Latihan fisik terdiri dari aktivitas aerobik dan aktivitas anaerobik. Latihan fisik aerobik maupun anaerobik dapat mengakibatkan pembentukan radikal bebas. Dalam keadaan ini dapat pula terjadi peningkatan sekresi BNP sebagai indikator terjadinya kerusakan otot. Brain Natriuretic Peptide (BNP) adalah Hormon type-B yang diproduksi di otot jantung yang akan terstimulasi ketika ventrikel jantung meregang dan mengalami tekanan yang berlebih atau sebagai sitoproteksi terhadap kelebihan volume dan peningkatan radikal bebas. Sampai saat ini masih relatif terbatas laporan mengenai perbandingan pengaruh latihan fisik aerobik dan anaerobik terhadap kadar BNP di otot jantung dalam melihat pengaruh positih dan negativenya terhadap fungsi jantung. Penelitian ini merupakan studi eksperimental dengan subjek penelitian pada 28 ekor tikus Wistar yang dibagi menjadi 2 kelompok (kelompok pembanding=P1, perlakuan aerobik 1 hari=P2, perlakuan anaerobik 1 hari=P3). Cara perlakuan latihan fisik menggunakan Animal Treadmill, dengan kecepatan 20 meter/menit selama 30 menit untuk latihan fisik aerobik, dan kecepatan 35 meter/menit selama 20 menit untuk latihan fisik anaerobik. Pemeriksaan kadar BNP dilakukan di Laboratorium Biokimia Fakultas Kedokteran Universitas Indonesia. Hasil uji statistik menunjukkan bahwa, tidak terdapat perbedaan yang bermakna kadar BNP otot jantung pada kelompok aerobic dan anaerobik yang diberi perlakuan 1 hari (sesaat). Akan tetapi dari hasil penelitian ini menunjukkan bahwa kadar BNP otot jantung pada latihan fisik anaerobik cenderung lebih tinggi dibandingkan dengan latihan fisik aerobik (6,00±26,13 vs  6,93±10,47) sebagai indikator terjadinya beban yang berlebih pada otot jantung. Dari penelitian ini dapat disimpulkan bahwa untuk memperbiki fungsi jantung, latihan fisik aerobik lebih baik daripada latihan fisik anaerobik.
Pengaruh Latihan Fisik Anaerobik Terhadap Kadar Laktat Plasma dan Kadar Laktat Jaringan Otot Jantung Tikus Wistar Flora, Rostika
Biomedical Journal of Indonesia: Jurnal Biomedik Fakultas Kedokteran Universitas Sriwijaya Vol 1, No 1 (2015)
Publisher : Fakultas Kedokteran Universitas Sriwijaya

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Abstract

Latihan fisik anaerobik adalah latihan  fisik yang dilakukan dalam durasi yang singkat dan dengan intensitas tinggi, proses metabolisme pembentukan energi tidak menggunakan oksigen. Energi dihasilkan dari pembentukan ATP melalui sumber energi yang berasal dari kreatinfosfat dan glikogen. Laktat merupakan produk akhir dari metabolisme anaerob. Latihan fisik anaerobik mengakibatkan terjadinya peningkatan kadar laktat  darah. Belum diketahui apakah peningkatan kadar laktat pada latihan fisik anaerobik juga terjadi pada otot jantung, mengingat jantung merupakan organ  yang bekerja keras selama latihan fisik. Penelitian ini bertujuan untuk mempengaruhi pengaruh latihan fisik anaerobik terhadap kadar laktat plasma dan kadar laktat jaringan otot jantung. Jaringan otot jantung berasal dari tikus yang diberi latihan  fisik anaerobik menggunakan treadmill selama 1,3,7 dan 10 hari tanpa hari istirahat. Kadar laktat diukur menggunakan Lactate Assay Kit dengan panjang gelombang pengukuran 450 nm untuk kadar laktat plasma dan 570 nm untuk  kadar laktat jaringan. Hasil penelitian menunjukkan bahwa terjadi peningkatan kadar laktat plasma sedangkan kadar laktat jaringan otot jantung terjadi penurunan dibandingkan kelompok kontrol. Peningkatan kadar laktat plasma pada hari ke-10 (2.26 ± 0.09 nmol/μL) hampir sama dengan hari pertama latihan fisik anaerobik (2.23 ± 0.28 nmol/μL), begitu pula penurunan kadar laktat jaringan otot jantung pada hari ke-10 (0.23 ± 0.03 nmol/μL) hampir sama dengan hari pertama latihan fisik anaerobik (0.22±0.00 nmol/μL). Terdapat perbedaan yang bermakna antara kadar laktat plasma dengan laktat jaringan otot jantung (p<0,05). Latihan fisik anaerobik mengakibatkan terjadinya peningkatan kadar laktat plasma dan penurunan kadar laktat jaringan otot jantung. Terdapat perbedaan yang bermakna antara kadar laktat plasma dengan laktat jaringan otot jantung pada tikus yang diberi pembebanan latihan fisik anaerobik (p<0,05).
Erratum: Chronic physical exercise increases a neurogenesis marker within hippocampus Zulkarnain, Mohammad; Andrianti, Septi; Flora, Rostika
Medical Journal of Indonesia Vol 27, No 3 (2018): September
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (116.864 KB) | DOI: 10.13181/mji.v27i3.3172

Abstract

[This corrects the article DOI: 10.13181/mji.v27i2.1685]
RELATIONSHIP BETWEEN LUNG FUNCTION AND INTERLEUKIN-6 (IL-6) LEVELS IN THE POPULATION IN THE VICINITY OF THE GANDUS RUBBER FACTORY AND THE SUKAWINATAN WASTE DISPOSAL SITE IN PALEMBANG Flora, Rostika; Zulkarnain, Mohammad; Adela, Novita
Biomedical Journal of Indonesia Vol 5, No 1 (2019)
Publisher : Fakultas Kedokteran Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bji.v5i1.7977

Abstract

Exposure to air pollution can interfere with the capacity of lung function and the body's immune system. Lung function capacity is the sum of two or more lung volumes. Interleukin-6 (IL-6) is an inflammatory mediator in respiratory pathogenesis. This study aims to determine the relationship between lung function and Interleukin-6 (IL-6) levels in the population around the Gandus Rubber Factory and Sukawinatan Landfill Palembang. The research design used is the type of correlation or explanatory that is examining the relationship between variables. A total of 72 samples were divided into 2 groups: groups in the Gandus Rubber Factory area and groups in the Sukawinatan Waste Disposal Site (Palembang). Lung function tests were measured using spirometry and Interleukin-6 levels were measured using ELISA examination. Chi square test results showed that there was no significant relationship (p value = 0.001) between lung function in the population around the Gandus Rubber Factory and Sukawinatan Waste Landfill. And there is no significant relationship (p value = 0.337) between Interleukin-6 (IL-6) levels in the population around the Gandus Rubber Factory and Sukawinatan Waste Landfill. Chi Square test results showed that there was no significant relationship (p value = 0.626) between lung function and Interleukin-6 (IL-6) levels in the population around the Gandus Rubber Factory. Chi Square test results showed that there was no significant relationship (p value = 1,000) between lung function and Interleukin-6 (IL-6) levels in the population around the Palembang Sukawinatan Landfill. Conclusions from the results of the study that there is no significant relationship (p <0.05) between levels of Interleukin-6 and Lung Function in the population around the Gandus Rubber Factory and Sukawinatan Landfill Palembang.
Kemitraan Puskesmas dan Sekolah dalam Pencegahan Malaria pada Anak Usia Sekolah di Seluma Bengkulu Novrinda, Hilza; Misnaniarti, Misnaniarti; Flora, Rostika; Zulkarnain, HM; Samwilson, Samwilson; Tanjung, Risnawati
Jurnal Promosi Kesehatan Indonesia Volume 15, No. 1 Januari 2020
Publisher : Master Program of Health Promotion Faculty of Public Health Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jpki.15.1.9-15

Abstract

Background: The partnership between Puskesmas (Community Health Center) and school to improve the health of school-age children known as the Trias school health unit. In Seluma only 7.69% of schools have a room for health school program. This study aims to explore the partnership between Puskesmas’ and schools in malaria prevention to school-age children in Seluma. Method: This study used qualitative research with phenomenological approach. It was conducted in April 2019. The main informants consisted of 5 teachers, from 5 elementary schools and 2 UKS staff Puskesmas from Puskesmas Puguk and Puskesmas Talang Tinggi, who have worked over than three years. The data collected by indepth interview, FGDs and observation. Results: The role of the UKS Puskesmas staffs was not optimal in carrying out the triad UKS activities. They only carry out routine UKS activities. Partnership between Puskesmas and schools has not been implemented optimally. It is still very limited action to cooperate the routine triad implementation from the Puskesmas and schools. A review needs to be done to assess the performance of the UKS, especially through healthy school competition activities.
The Effect of Saliva Zink Levels with Nutrition Status of Elementary School Children in Air Beliti Puskesmas Year 2021 Aini, Putri Citra Qurrothu; Flora, Rostika; Zulkarnain, M.; Ocktariana, Ocktariana; Tanjung, Risnawati
Budapest International Research and Critics Institute (BIRCI-Journal): Humanities and Social Sciences Vol 4, No 3 (2021): Budapest International Research and Critics Institute August
Publisher : Budapest International Research and Critics University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33258/birci.v4i3.2202

Abstract

The impaired nutrition in children is a very important problem because it has a direct impact on the growth and development of children. Malnutrition in children can lead to micronutrient deficiencies including zinc. Zinc is an important mineral found in every cell and is needed to support the body's immune system, heal wounds, help the ability to taste and smell and is needed in the DNA synthesis process. The purpose of this study was to examine the relationship between salivary zinc with the nutritional status of elementary school children in the Air Beliti Public Health Center work area. Methods: This study is an observational study with a cross-sectional design. The study was conducted on 76 respondents who met the inclusion criteria. Measurement of Height for Age to determine the nutritional status of children and examination of salivary zinc levels with the ELISA method to determine salivary zinc levels. The data was analyzed using the Spearman correlation test. Results: The results of the measurement of nutritional status showed that 46.1% of children were stunted and 53.9% of children had normal nutritional status. The results of the measurement of salivary zinc levels showed that the average salivary zinc level in stunted children was 1.96 mmol/L, while in children who were not stunted, it was 2.43 mmol/L. Salivary zinc levels in stunted children were lower than in children who were not stunted. Conclusions and Suggestions: There is an effect of saliva zinc examination on the nutritional status of schoolchildren. It is necessary to increase the intake of nutrients, especially zinc, in children so that their nutritional status is not disturbed. Also, check the nutritional status of children by adding variables that can describe the nutritional status of children in accordance with their development and growth.