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Journal : Journal of Indonesian Medical Laboratory and Science (JoIMedLabS)

EFEKTIVITAS VARIASI KONSENTRASI TEPUNG TALAS SEBAGAI MEDIA ALTERNATIF PERTUMBUHAN JAMUR Candida albicans Marchamtia Sarah Nur Awalia Fajari; Muhammad Taufiq Qurrohman
Journal of Indonesian Medical Laboratory and Science Vol 2 No 2: Oktober 2021
Publisher : Asosiasi Institusi Pendidikan Tinggi Teknologi Laboratorium Medik Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53699/joimedlabs.v2i2.56

Abstract

Taro flour (Colocasia esculenta (L.) Schott) has 77%% carbohydrate. A high carbohydrate source has the potential to be used as an alternative media in supporting the examination of Candida albicans fungal culture. Candida albicans is one of the fungi causing candidiasis. Therefore, this study aims to see the effectiveness of the variations of taro flour concentrations as an alternative media to the growth of fungus Candida albicans. The type of the study was experimental with a post test only control group design. The subjects of the study were taro flour media with concentrations of 0,12%, 0,24%, and 0,36% tested on fungus Candida albicans, which was inoculated by spread plate with PDA (Potato Dextrose Agar) media used as control. The data collection was performed by calculating the colony of Candida albicans, which grew in each media. The mean colony of each media on PDA, taro flour 0,12%, 0,24%, 0,36% were 15.5 colonies, 18.8 colonies, 19.5 colonies, and 20.8 colonies, respectively. The effectiveness test showed that all taro flour concentrations have a percentage more than 100%. The variation of taro flour has the growth effect of fungus Candida albicans. Thus, the higher the concentration of taro flour media, the more colony the number of Candida albicans grew. The effectiveness test showed that all taro flour concentrations was classified as very effective.
PENGARUH VARIASI KONSENTRASI SARI PATI BUAH SUKUN SEBAGAI ALTERNATIF MEDIA SEMI SINTETIK PADA PERTUMBUHAN JAMUR Candida albicans Reza Yuliana; M. Taufiq Qurrohman
Journal of Indonesian Medical Laboratory and Science Vol 3 No 1: April 2022
Publisher : Asosiasi Institusi Pendidikan Tinggi Teknologi Laboratorium Medik Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53699/joimedlabs.v3i1.67

Abstract

Gold standard examination Candida sp.  is a culture test.  The abundance natural resources in Indonesia encourages researchers to take advantage the wealth of natural resources to create alternative semi-synthetic media for mushroom growth from breadfruit.  The purpose of this study was determining the effective concentration of alternative semi-synthetic media of breadfruit essence as growth medium for the fungus Candida albicans. This research is experimental study. Observations were made macroscopically and confirmed microscopically. The results showed that average growth of the colonies on PDA media was 15.50, for the alternative breadfruit media the average of colonies was 24.50 (100% concentration), 19.75 (90% concentration), 19.50 (80% concentration), 16.00 (70% concentration), 15.50 (60% concentration). The results of the ANOVA test obtained value <0.05, so H1 was accepted there was a significant difference in the average growth of fungi in the media of breadfruit essence. The conclusion from this research is that the concentration of 60% breadfruit essence is the effective for growing Candida albicans.