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Zakiah Heryawati Manaf
Laboratorium Mikrobiologi Fakultas Kedokteran Hewan Universitas Syiah Kuala, Banda Aceh
Health Professions

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KONTAMINASI BAKTERI KOLIFORM PADA AIR MINUM ISI ULANG DI DESA ILIE KECAMATAN ULEE KARENG KOTA BANDA ACEH (Contamination of Coliform in Refill Water in Ilie Village, Ulee Kareng, Banda Aceh) M. Iqbal Pratama Sekedang; Zakiah Heryawati Manaf; Darmawi Darmawi; Faisal Jamin; Mahdi Abrar; Razali Razali
Jurnal Medika Veterinaria Vol 10, No 1 (2016): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (129.513 KB) | DOI: 10.21157/j.med.vet..v10i1.4046

Abstract

The purpose of this study was to find out whether the refill water (AMIU) was contaminated with coliform or not. The methods used were presumptive, confirmed, and completed test. Sample of this study was refill water (AMIU) from five refill water shops located in Ilie village, sub-district of Ulee Kareng, Banda Aceh. The sample was taken 3 times with interval period of one week. Sample was culture in lactose broth and incubated at 37° C for 48 hours. If it did not form gas in Durham tube, presumptive test result was considered negative and the samples were not proceed to the other test. The data was presented using descriptive analysis. It could be concluded that AMIU from 5 shops in Ilie Ulee Kareng, Banda Aceh is negatively contaminated with coliform bacteria.
ISOLASI BAKTERI Salmonella sp. PADA FESES ANAK AYAM BROILER DI PASAR ULEE KARENG BANDA ACEH (Isolation of Salmonella sp. in Feces of Broiler Chicks at Ulee Kareng Market Banda Aceh) Afriyani Afriyani; Darmawi Darmawi; Fakhrurrazi Fakhrurrazi; Zakiah Heryawati Manaf; Mahdi Abrar; Winaruddin Winaruddin
Jurnal Medika Veterinaria Vol 10, No 1 (2016): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (93.039 KB) | DOI: 10.21157/j.med.vet..v10i1.4047

Abstract

The research aimed to isolate Salmonella sp.in feces of broiler chicks. Fifteen feces samples of broiler chick were obtained from Ulee Kareng Market in Banda Aceh. Chick cloaca was swabbed using a sterile cotton swab, put into the microtube, and brought to the Microbiology Laboratory of Veterinary Medicine Faculty, Syiah Kuala University. The samples were incubated in selenite cystine broth (SCB) medium, cultured in salmonella shigella agar (SSA) and separated colonies were inoculated in nutrien agar (NA) at 37° C for 24 hours. Bacteria stained with Gram staining then tested biochemically. The result showed that Salmonella sp. were identified in 9 out of 15 feces chicks samples. It ca be concluded that broiler chick in Ulee Kareng Market Banda Aceh contaminated with bacteria Salmonella sp.
25. Effect of Maintenance Temperature and Shelf Life of Tilapia (Oreochromis niloticus) Fillet Stored in Temperature -20 C on Total Count Bacteria Nurdiani Muliana Sitakar; Nurliana N; Faisal Jamin; Mahdi Abrar; Zakiah Heryawati Manaf; Sugito S
Jurnal Medika Veterinaria Vol 10, No 2 (2016): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (125.367 KB) | DOI: 10.21157/j.med.vet..v10i2.4387

Abstract

This study aims to determine the effect of storage time on the total number of bacteria in nile tilapia (Oreochromis niloticus) flesh stored at a temperature of 20 C and at different retention time. This study used split plot completely randomized design (CRD) with 2 treatment maintenance temperature of 29 C and 35 C. Experimental animals used were 30 nile tilapia fish grouped into five storage group namely 0 (P0), 7 (P1), 14 (P2), 21 (P3), and 28 (P4) days. The results showed that total number of bacteria found in fish flesh was higher than normal standard based on ISO 7388:2009 which was 5x105 cfu/g. The average of total numbers of bacteria in flesh of nile tilapia fish reared in 29 C on P0, PI, PII, PIII, and PIV were 6.55±0.19, 6.99±0.07, 6.96±0.08, 6.96±0.28, and 7.18±0.15, respectively. While the average of total numbers of bacteria in flesh of nile tilapia fish maintained in temperature of 35 C were 6.68 ± 0.10, 7.00±0.05, 7.19±0.12, 7.24±0.12, and 7.35±0.09, respectively. Total number of bacteria in nile tilapia flesh was affect significantly (P0.05) by temperature and storage temperature maintenance. In conclusion, storage time and different tempera ure maintenance of nile tilapia can increase total amount of bacteria on fish flesh.
PERKEMBANGAN DAN GAMBARAN ANATOMIS LARVA INFEKTIF (L3) Haemonchus contortus YANG DIBIAKKAN DENGAN VERMICULLITE (Development and Anatomical Description of Infektive Larvae (L3) Haemonchus contortus Cultured with Vermicullite) Muhammad Hambal; Rahmi Sulastri Mukhtar; Muhammad Hanafiah; Yudha Fahrimal; Winaruddin Winaruddin; Zakiah Heryawati Manaf
Jurnal Medika Veterinaria Vol 10, No 1 (2016): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v10i1.4043

Abstract

This study was aimed at obtaining basic information about bioecology of Haemonchus contortus, related to larval development and anatomical description of infective larvae (L3). In this study, the ova of Haemonchus contortus were harvested out of the intestines of goats, those were slaughtered in municipal slaughterhouse in Kampung Jawa Banda Aceh. The ova of Haemonchus contortus were then cultured using vermiculite as media, and observed daily until the larvae reached 3rd stage larvae. The result revealed that the larvae need 7 days to reach from eggs into infective larvae. Larvae obtained has uniform morphology, with a body diameter of 22 μm, tail length up to 55 μm hyaline, esophagus posterior to the tail of 592 μm, length of esophagus anterior to posterior esophagus 145 μm, and a total body length of larvae 737 μm.
Co-Authors . Nurliana Afriyani Afriyani Darmawi Darmawi Faisal Jamin Fakhrurrazi Fakhrurrazi M. Iqbal Pratama Sekedang Mahdi Abrar Muhammad Hambal Muhammad Hanafiah Nurdiani Muliana Sitakar Rahmi Sulastri Mukhtar Razali Razali Sugito Sugito Winaruddin Winaruddin Yudha Fahrimal