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All Journal ACTA VETERINARIA INDONESIANA Jurnal Sain Veteriner Jurnal Veteriner Jurnal Agripet Jurnal Medika Veterinaria Jurnal Ilmiah Mahasiswa Veteriner Jurnal Kedokteran Hewan The International Journal of Tropical Veterinary and Biomedical Research

Mahdi Abrar

Unknown Affiliation

Author-ID : 218391

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

Published : 33 Documents Claim Missing Document

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Aktivitas Antibiotik terhadap Biofilm Staphylococcus aureus Isolat Preputium Sapi Aceh Dewi, Maryulia; Darmawi, Darmawi; Nurliana, Nurliana; Karmil, T. Fadrial; Helmi, T. Zahrial; Fakhrurrazi, Fakhrurrazi; Erina, Erina; Abrar, Mahdi; AK, M. Daud; Admi, Masda
Jurnal Sain Veteriner Vol 38, No 1 (2020): April
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Biofilm is one of parameters for determining pathogenic pathways of a bacterium. Bacterial virulence activities can be attenuated by antibiotics with inhibiting biofilm. The aim of this study was to examine the characteristics and activities of antibiotics against the biofilm of Stapylococcus aureus isolate which taken from Aceh cattle prepuce. The study was exploratory and experimental, using a Completely Randomized Design (RAL) factorial pattern using three types of antibiotics, three concentrations and replications. Antibiotics were tetracycline, oxytetracycline and phosphomycin with concentrations of 10%, 20% and 30% (mg/mL), respectively. Therefore, the aim of this study was to examine the characteristics and activities of antibiotics to the biofilm of Stapylococcus aureus from Aceh cattle prepuce isolate. U-bottom polystyrene 96 wells microplate and Nutrient Broth (NB) medium was used to biofilm bacterial while biofilm was stained on crystals violet 1%. Optical Density (OD) of biofilm were Î» 595 nm. Data were analyzed by using Analysis of Variance (ANOVA). The results showed that the activity of Staphylococcus aureus was moderately positive, i.e 2,667cell/Î¼L. It showed that antibiotics and concetrations have significant effect (P<0.05) to inhibit biofilm staphylococcus aureus. In additioon, tetracycline 30% (mg / mL) has the best ability to inhibit biofilms from 52,18% to 1,262 cell/Î¼L. Based on that fact, it can be concluded that the virulence of Staphylococcus aureus was moderate positive to be false positive.

Isolasi dan Identifikasi Aspergillus Sp. pada Kantung Hawa Puyuh (Cortunix Japonica) Mega Cempaka Putri; Erina Erina; Mahdi Abrar; M. Daud AK
Acta VETERINARIA Indonesiana Vol. 9 No. 2 (2021): Juli 2021
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avi.9.2.134-142

Aspergillosis merupakan penyakit yang disebabkan oleh jamur Aspergillus sp. yang dapat menyerang puyuh muda secara akut dan sebagai penyakit menahun pada puyuh dewasa. Penelitian ini bertujuan untuk mengisolasi dan mengidentifikasi Aspergillus sp. pada kantung hawa puyuh (Coturnix japonica). Sampel yang digunakan dalam penelitian ini adalah 35 kantung hawa puyuh yang tidak produktif lagi (afkir) diambil secara acak. Isolasi dan identifikasi Aspergillus sp. dilakukan sesuai dengan menggunakan metode Thompson. Sampel dicuci dengan aquadest steril berisi antibiotic gentamicin selanjutnya ditanamkan pada media Sabouraud’s Dextrose Agar (SDA) kemudian diinkubasikan pada suhu kamar selama 3-7 hari. Pertumbuhan koloni Aspergillus sp. diamati morfologinya secara makroskopis dan mikroskopis. Data yang diperoleh dianalisis secara deskriptif. Hasil pemeriksaan menunjukkan bahwa Aspergillus sp. dapat diisolasi dan diidentifikasi pada 24 dari 35 sampel kantung hawa. Berdasarkan hasil penelitian dapat disimpulkan bahwa 68,57% sampel yang diperiksa positif terinfeksi Aspergillus sp. dengan persentase masing-masing spesies Aspergillus fumigatus 40%, Aspergillus flavus 25,7%, dan Aspergillus niger 17,1%.

Deteksi Antibodi terhadap Virus Newcastle Disease pada Burung Trucukan (Pycnonotus goiavier) Erina Erina; Hanni Aninaidu; Zuhrawaty Zuhrawaty; Etriwati Etriwati; Abdullah Hamzah; Mahdi Abrar; M. Daud AK
Acta VETERINARIA Indonesiana Vol. 9 No. 3 (2021): November 2021
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avi.9.3.173-178

Burung trucukan (Pycnonotus goiavier) merupakan salah satu burung liar yang sering dipelihara karena kicauannyayang merdu. Infeksi ND dapat berupa infeksi yang akut atau kronis yang menyerang burung trucukan dan jenis burunglainnya. Burung liar merupakan salah satu sumber virus ND yang dapat menyebarkan virus ke unggas peliharaan.Penelitian ini bertujuan untuk mengetahui titer antibodi terhadap penyakit tetelo pada burung trucukan liar. Sampel yangdigunakan adalah serum darah dari 50 ekor burung trucukan liar. Parameter yang diamati adalah titer antibodi dalamserum berdasarkan metode Hirst menggunakan uji Haemagglutination Inhibition (HI) untuk melihat titer antibodi. Untukmenghitung titer virus maka uji Haemaglutination (HA) dilakukan sebelumnya. Untuk memastikan titer antigen yangdigunakan adalah 4HAU dilkukan juga back titrasi sehingga didapatkan nilai virus optimal yang akan dipakai pada uji HI.Hasil penelitian menunjukkan 45 dari 50 sampel (90%) mempunyai antibodi positif terhadap ND, dengan titer berkisar 21-29. Berdasarkan hasil yang diperoleh dapat disimpulkan bahwa 90% burung trucukan di Kabupaten Aceh Besar sudahpernah terpapar ND, tetapi hanya 80% yang memiliki titer antibodi yang dapat melingdungi terhadap serangan virus ND.Oleh sebab itu dapat disimpulkan bahwa burung trucukan liar dengan titer antibodi yang protektif berarti sudah pernahterpapar virus ND sebelumnya akan berpotensi sebagai pembawa virus yang dapat ditularkan ke unggas peliharaanmasyarakat di sekitarnya.

Deteksi Antibodi Serum Terhadap Virus Avian influenza pada Ayam Buras Darmawi Darmawi; Zakiyah Heryawati Manaf; Darniati Darniati; Fakhrurrazi Fakhrurrazi; Mahdi Abrar; Erina Erina
Jurnal Agripet Vol 12, No 1 (2012): Volume 12, No. 1, April 2012
Publisher : Agricultural Faculty

Detection on Serum Antibodies of Native Chickens to Avian influenza VirusABSTRACT. An important approach of controlling against Avian Influenza should be determined to detect the antibody titres of bird flu caused by Influenza virus H5N1 in Indonesia. The aim of the present study was to detect the antibodies to Avian Influenza in serum of native chickens. This study utilized 123 serum samples collected from the axilaris vein (left or right) of native chickens. Antibody titres were examined using Hemaglutination Inhibition (HI). The result showed that indication of natural infection by Avian Influenza (H5N1) in native chickens, as shown that out of 123 serum samples, 16 (13,01%) were tested positive by HI, while only 10 (8,13%) were tested protective to Avian influenza infection. Based on the results we obtained, a conclusion that natural infection by Avian influenza virus stimulated variety level of formation antibody titres in native chickens.

Seroprevalensi Avian influenza H5N1 pada Unggas di Kabupaten Aceh Utara Darmawi Darmawi; Darniati Darniati; Maryulia Dewi; Fakhrurrazi Fakhrurrazi; Mahdi Abrar; Erina Erina
Jurnal Agripet Vol 13, No 2 (2013): Volume 13, No. 2, Oktober 2013
Publisher : Agricultural Faculty

Seroprevalence of avian influenza H5N1 in birds in north aceh districtABSTRACT. Avian influenza virus H5N1 infections are an important cause of diseases in humans and several animal species, including birds. The present study conducted to investigate the seroprevalence Avian Influenza H5N1 in native birds from 15 sub-districts of North Aceh. This study utilized 1108 serum samples collected from the axilaris vein (left or right) of birds. The standard Hemaglutination Inhibition (HI) assay was conducted at Microbiology Laboratory Faculty of Veterinary Medicine of Syiah Kuala University to determined serum antibody possitive or negative reaction against Avian influenza H5N1. The result showed that seroprevalence Avian influenza H5N1 virus was 4,7 % in North Aceh District. There were nine sub-districts were tested positively by HI test. However, the serum collected from six sub-districts did not react (negative) against Avian influenza H5N1. Based on the results we obtained, a conclusion that natural infection by Avian influenza virus in native birds occured in part of North Aceh District.

KONTAMINASI BAKTERI KOLIFORM PADA AIR MINUM ISI ULANG DI DESA ILIE KECAMATAN ULEE KARENG KOTA BANDA ACEH (Contamination of Coliform in Refill Water in Ilie Village, Ulee Kareng, Banda Aceh) M. Iqbal Pratama Sekedang; Zakiah Heryawati Manaf; Darmawi Darmawi; Faisal Jamin; Mahdi Abrar; Razali Razali
Jurnal Medika Veterinaria Vol 10, No 1 (2016): J. Med. Vet.
Publisher : Universitas Syiah Kuala

The purpose of this study was to find out whether the refill water (AMIU) was contaminated with coliform or not. The methods used were presumptive, confirmed, and completed test. Sample of this study was refill water (AMIU) from five refill water shops located in Ilie village, sub-district of Ulee Kareng, Banda Aceh. The sample was taken 3 times with interval period of one week. Sample was culture in lactose broth and incubated at 37° C for 48 hours. If it did not form gas in Durham tube, presumptive test result was considered negative and the samples were not proceed to the other test. The data was presented using descriptive analysis. It could be concluded that AMIU from 5 shops in Ilie Ulee Kareng, Banda Aceh is negatively contaminated with coliform bacteria.

ISOLASI BAKTERI Salmonella sp. PADA FESES ANAK AYAM BROILER DI PASAR ULEE KARENG BANDA ACEH (Isolation of Salmonella sp. in Feces of Broiler Chicks at Ulee Kareng Market Banda Aceh) Afriyani Afriyani; Darmawi Darmawi; Fakhrurrazi Fakhrurrazi; Zakiah Heryawati Manaf; Mahdi Abrar; Winaruddin Winaruddin
Jurnal Medika Veterinaria Vol 10, No 1 (2016): J. Med. Vet.
Publisher : Universitas Syiah Kuala

The research aimed to isolate Salmonella sp.in feces of broiler chicks. Fifteen feces samples of broiler chick were obtained from Ulee Kareng Market in Banda Aceh. Chick cloaca was swabbed using a sterile cotton swab, put into the microtube, and brought to the Microbiology Laboratory of Veterinary Medicine Faculty, Syiah Kuala University. The samples were incubated in selenite cystine broth (SCB) medium, cultured in salmonella shigella agar (SSA) and separated colonies were inoculated in nutrien agar (NA) at 37° C for 24 hours. Bacteria stained with Gram staining then tested biochemically. The result showed that Salmonella sp. were identified in 9 out of 15 feces chicks samples. It ca be concluded that broiler chick in Ulee Kareng Market Banda Aceh contaminated with bacteria Salmonella sp.

25. Effect of Maintenance Temperature and Shelf Life of Tilapia (Oreochromis niloticus) Fillet Stored in Temperature -20 C on Total Count Bacteria Nurdiani Muliana Sitakar; Nurliana N; Faisal Jamin; Mahdi Abrar; Zakiah Heryawati Manaf; Sugito S
Jurnal Medika Veterinaria Vol 10, No 2 (2016): J. Med. Vet.
Publisher : Universitas Syiah Kuala

This study aims to determine the effect of storage time on the total number of bacteria in nile tilapia (Oreochromis niloticus) flesh stored at a temperature of 20 C and at different retention time. This study used split plot completely randomized design (CRD) with 2 treatment maintenance temperature of 29 C and 35 C. Experimental animals used were 30 nile tilapia fish grouped into five storage group namely 0 (P0), 7 (P1), 14 (P2), 21 (P3), and 28 (P4) days. The results showed that total number of bacteria found in fish flesh was higher than normal standard based on ISO 7388:2009 which was 5x105 cfu/g. The average of total numbers of bacteria in flesh of nile tilapia fish reared in 29 C on P0, PI, PII, PIII, and PIV were 6.55±0.19, 6.99±0.07, 6.96±0.08, 6.96±0.28, and 7.18±0.15, respectively. While the average of total numbers of bacteria in flesh of nile tilapia fish maintained in temperature of 35 C were 6.68 ± 0.10, 7.00±0.05, 7.19±0.12, 7.24±0.12, and 7.35±0.09, respectively. Total number of bacteria in nile tilapia flesh was affect significantly (P0.05) by temperature and storage temperature maintenance. In conclusion, storage time and different tempera ure maintenance of nile tilapia can increase total amount of bacteria on fish flesh.

IDENTIFIKASI BAKTERI Staphylococcus aureus PADA PREPUTIUM DAN VAGINA KUDA (Equus caballus) (Identification of Staphylococcus aureus in Preputium and Vagina of Horses (Equus caballus)) Yulina Rahmi; Darmawi Darmawi; Mahdi Abrar; Faisal Jamin; Fakhrurrazi Fakhrurrazi; Yudha Fahrimal
Jurnal Medika Veterinaria Vol 9, No 2 (2015): J. Med. Vet.
Publisher : Universitas Syiah Kuala

This research aims to identify the Staphylococcus aureus (S. aureus) bacteria from preputium and vagina of horses. Five swabs preputium and 5 swabs vagina samples from 3-9 years olds horse in the village of Bebangka, Gelelungi, Reloop, and Kelapeng, Pegasing Aceh Tengah District were used in this study. Preputium and vagina swabs of horses were dipped into reaction tube containing Nutrient Broth (NB). Staphylococcus aureus was identified on manitol salt agar (MSA). Separated colony colored bright yellow on the media were tested for Gram staining, hemolysis, catalase, and sugar test (glucose and manitol). The result showed that the isolates growth on MSA were colored bright yellow, Gram positive, β hemolisis, and catalase positive. The isolates fermented manitol and glucose. Isolates identified from horses preputium and vagina showed the characteristic of S. aureus. In conclusion S. aureus bacteria were positively identified from all of swabs samples, both in preputium and vagina of horses.Key words: Staphylococcus aureus, horse, preputium, vagina

11. Bursa Body Weight Ratio in ALPU Chickens Vinky Claudya Fransiska; Erina Erina; Mahdi Abrar; M Aman Yaman; Ummu Balqis; M. Jalaluddin
Jurnal Medika Veterinaria Vol 14, No 1 (2020): J.Med.Vet
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v14i1.10290

This study was aimed to determine ratio of bursa body weight in ALPU chickens. The samples used were 72 ALPU chickens consisting of 36 males and 36 females aged 1-18 weeks. This study was observed in every week by weighing the weight of ALPU chickens as much as 2 males and 2 females, then chickens slaughtered. After that, Fabricius's bursa on each chicken was separated from the chicken body part and weighed using a digital balance sheet. The results of this study showed that the maximal ratio in male ALPU chickens was 0.0032 at the age 2 weeks, while the maximal ratio in female ALPU chickens was 0.0036 at the age 4 weeks. From the results of the analysis using t-test was concluded that bursa body weight ratio in male and female ALPU chickens during starter to grower group was significantly different (p0.05). The grower to finisher group was not significantly different (p0.05).

Co-Authors - Fakhrurrazi . Darmawi . Darniati . Nurliana Abd. Rasyid Syamsuri Abdullah Hamzah Admi, Masda Afriyani Afriyani AK, M. Daud Arman Sayuti Bambang Pontjo Priosoeryanto Budianto Panjaitan Budianto Panjaitan Chairul Saputra Siregar chiara giri puspa cut nila thasmi Darmawi Darmawi Darmawi Darmawi Dwinna Aliza Erdiansyah Rahmi Erdiansyah Rahmi Erina Erina Erina Erina Erina Erina Erina Erina Erina Erina Erina Erina Erina Erina erma yusintha ramdhania Etriwati E Fachriyan Hasymi Pasaribu Fadli A. Gani Faisal Jamin Faisal Jamin Fakhrurrazi Fakhrurrazi Farida Athaillah Gholib Gholib Hafizuddin Hafizuddin Hanni Aninaidu Helmi, T. Zahrial Herrialfian . I wayan Teguh Wibawan ismail ismail ismail Juli Melia Juli Melia Khairiah Khairiah Khairul Rizal Liza Setia Joni M Aman Yaman M Nur Salim M. Iqbal Pratama Sekedang Maryulia Dewi Maryulia Dewi Maryulia Dewi Mega Cempaka Putri Mirnawati Soedarwanto Mirnawati Soedarwanto Muhammad Jalaluddin Mulyadi Adam Muslim Akmal Nanda Yulian Syah Nellita Meutia Nurdiani Muliana Sitakar NURLIANA NURLIANA Rastina Rastina Rastina Rastina Razali Daud Razali Razali Rezi Maghfira roslizawaty roslizawaty Rosmaidar Rosmaidar Sari, Wahyu Eka Sri Wahyuni Sugito Sugito Suryani Suryani Syafruddin Syafruddin Syafruddin Syafruddin Syafruddin Syafruddin T Reza Ferasyi T Zahrial Helmi T. Armansyah T. Fadrial Karmil T. Fadrial Karmil Tongku Nizwan Siregar Tongku Nizwan Siregar Tongku Nizwan Siregar Ummu Balqis Vinky Claudya Fransiska Winaruddin Winaruddin Yudha Fahrimal Yulina Rahmi Zakiah Heryawati Manaf Zakiyah Heryawati Manaf Zuhrawati Zuhrawati Zuhrawaty Zuhrawaty

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