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The Pattern of Carbohydrate Distribution of Accessory Sex Glands in Male Muntjac (Muntiacus muntjak muntjak) Sri Wahyuni; Boni Anggara; Muslim Akmal; Hamny Hamny; Mustafa Sabri
Jurnal Medika Veterinaria Vol 8, No 2 (2014): J. Med. Vet.
Publisher : Universitas Syiah Kuala

The objective of this study was to investigate the pattern of carbohydrate distribution of accressory sex glands in male muntjac (Muntiacus muntjak muntjak) in the velvet antler stage. In macroanatomy observation, muntjac accessory sex glands consist of the ampulla, vesicular gland, pars diseminate prostat, and bulbourethral gland. The observation of neutral carbohydrate distribution has done by periodic acid Schiff (PAS) staining. In addition, acid carbohydrate was observed by using alcian blue (AB) pH2,5 staining. Result of this research showed that neutral and acid carbohydrate scattering in each accessories sex gland with different pattern. Neutral carbohydrate with medium (++) and high (+++) intensity reaction was found in the ampulla, vesicularis gland, and bulbourethral gland and strong in prostat gland. The acid carbohydrate with low (+) and medium (++) intensity reaction was observed in the ampulla, low in vesicularis gland, and negative in prostat and bulbourethral glands. In conclusion, the neutral carbohydrate was found dominantly on surface of all accessory sex glands of male muntjac in velvet antler stage with highest concentration and intensity of colour in prostat gland.Key words: carbohydrate, accessory sex glands, muntjac (Muntiacus muntjak muntjak)

PENGARUH PEMBERIAN EKSTRAK KULIT BATANG JALOH (Salix tetrasperma Roxb) DAN SERBUK DAUN SINGKONG (Manihot esculenta Crantz) TERHADAP PERTAMBAHAN BOBOT BADAN AYAM BROILER (Effect of Willow (Salix tetrasperma Roxb) Bark Extract and Cassava (Manihot esculenta Crantz) Leaf Powder Supplementation on Weight Gain of Broiler Chickens) Hanum Harfinda; Rinidar Rinidar; T. Armansyah; Sugito Sugito; Mustafa Sabri; Winaruddin Winaruddin
Jurnal Medika Veterinaria Vol 10, No 1 (2016): J. Med. Vet.
Publisher : Universitas Syiah Kuala

This study’s purpose is to determine the effect of supplementation of willow bark extract (1000 mg/L) in drinking water and 15% cassava leaf powder in feed on broiler weight gain. This study was designed using completely randomized design (CRD) in which 20 broiler chickens were divided into four treatment groups with five broiler per each treatment. Control group (P0) was only given commercial feed, P1 (commercial feed + cassava leaf powder), P2 (commercial feed + willow bark extract), and P3 (commercial feed + cassava leaf powder and willow bark extract). Data were analyzed using analysis of variance and followed by Duncan test. The results showed that P2 significantly different (P0.01) compare to P0 and P3, and different (P0.05) from P1. Group P1 was different (P0.05) from group P3, but P3 showed no significant different (P0.05) to P0 on weight gain. whereas feed intake was similar among treatments, however food conversion was differ among treatments. In conclusion, combination of willow bark extract and cassava leaf powder could not increase broiler chickens weight gain.

PROLIFERASI DAN DIFERENSIASI SEL TULANG TIKUS DALAM MEDIUM KULTUR IN VITRO YANG MENGANDUNG EKSTRAK BATANG Cissus quadrangula Salisb. (SIPATAH-PATAH) Ita Djuwita; Irma Amalia Pratiwi; Adi Winarto; Mustafa Sabri
Jurnal Kedokteran Hewan Vol 6, No 2 (2012): September
Publisher : Universitas Syiah Kuala

Penelitian mengenai pengaruh ekstrak batang Cissus quadrangula Salisb. (sipatah-patah) terhadap tingkat proliferasi dan diferensiasi sel-sel tulang tikus (Sprague Dawley) prepuber umur empat minggu menggunakan sistem kultur in vitro. Sel-sel tulang dikultur dalam medium dulbecco’s modified eagle’s medium (DMEM) yang diberi tambahan newborn calf serum (NBCS) 10%, non essential amino acid (NEAA) 10%, NaHCO3, ITS 1 µl/ml (mengandung insulin 5 μg/ml, transferin 10 μg/ml, selenium 5 μg/ml; Sigma St Louis USA), dan 50 µg/ml gentamisin (mDMEM), dengan dan tanpa ekstrak Cissus quadrangula (CQ). Penelitian terdiri atas lima perlakuan yakni kontrol positif (mDMEM + deksametason 10-8 M), kontrol negatif (mDMEM), dan tiga konsentrasi CQ: mDMEM + CQ 0,3 mg/ml; mDMEM + CQ 0,6 mg/ml; dan mDMEM + CQ 1,2 mg/ml. Kultur dilakukan dalam inkubator CO2 5%, pada suhu 37° C selama tujuh hari. Parameter yang diamati adalah konsentrasi sel, komposisi, diameter osteoblas, dan diameter osteosit. Konsentrasi sel dihitung menggunakan hemositometer Newbauer. Komposisi osteoblas dan osteosit ditentukan berdasarkan pengamatan morfologi di bawah mikroskop cahaya. Diameter sel diukur menggunakan eyepiece micrometer. Data dianalisis menggunakan analisis varians dan uji Duncan. Hasil penelitian menunjukkan bahwa pemberian ekstrak Cissus quadrangula Salisb. pada konsentrasi 0,3 mg/ml; 0,6 mg/ml; dan 1,2 mg/ml secara signifikan dapat meningkatkan proliferasi sel tulang; dan pada konsentrasi 0,6 mg/ml mampu menginduksi diferensiasi osteoblas menjadi osteosit (P0,05). Disimpulkan bahwa pemberian ekstrak Cissus quadrangula pada konsentrasi 0,6 mg/ml ke dalam medium kultur dapat meningkatkan proliferasi dan diferensiasi osteoblas.

STUDI ANATOMIS DAN HISTOLOGIS PANKREAS BIAWAK AIR (Varanus salvator) (Anatomical and Histological Study of the Pancreas of Water Monitor Lizard (Varanus salvator)) Hamny Hamny; Muhammad Iqbal; Sri Wahyuni; Mustafa Sabri; M. Jalaluddin; Rinidar Rinidar
Jurnal Kedokteran Hewan Vol 10, No 2 (2016): September
Publisher : Universitas Syiah Kuala

The aim of this research was to determine the anatomy and histology of pancreas of water monitor lizard (Varanus salvator). Two pancreas were used in this research (a male and a female). Water monitor lizard was euthanized using chloroform and subsequently necropsied to collect the pancreas. The pancreas was washed in 0.9 % NaCl solution. Anatomical observations were carried out including location, shape, and color of pancreas, also the length and weight measurements. Then, pancreas was fixed with 4% paraformaldehyde for 7 days to proceed to histological preparation and hematoxylin eosin (HE) staining. The results of the anatomical observations showed that pancreas of water monitor lizard have two lobes, dorsal lobe (splenic lobe) and ventral lobe (duodenal lobe). Dorsal lobe which was in oval form adhered with the spleen, and ventral lobe was located in the cranial of intestines with the form of long spherical. Both of pancreas lobes were white-yellow color with the length of 1.45±0.64 cm (dorsal lobe) and 7.00±0.42 cm (ventral lobe). Weight of dorsal lobe was 0.15±0.01 g and ventral lobe was 6.35±2.30 g. The histological observations found the acinar cells, ducts, and blood vessels in both of pancreas lobes. Additionally, the similar structure of Langerhans islet only found at the ventral lobe of pancreas. In conclusion, pancreas of water monitor lizard consists of two lobes which located differently. Ventral lobe is larger than the dorsal lobe. Both lobes have similar histological structure except the Langerhans islets are only appeared in the ventral lobe.

PROLIFERASI DAN DIFERENSIASI SEL TULANG TIKUS DALAM MEDIUM KULTUR IN VITRO YANG MENGANDUNG EKSTRAK BATANG Cissus quadrangula Salisb. (SIPATAH-PATAH) Ita Djuwita; Irma Amalia Pratiwi; Adi Winarto; Mustafa Sabri
Jurnal Kedokteran Hewan Vol 6, No 2 (2012): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v6i2.295

Penelitian mengenai pengaruh ekstrak batang Cissus quadrangula Salisb. (sipatah-patah) terhadap tingkat proliferasi dan diferensiasi sel-sel tulang tikus (Sprague Dawley) prepuber umur empat minggu menggunakan sistem kultur in vitro. Sel-sel tulang dikultur dalam medium dulbecco’s modified eagle’s medium (DMEM) yang diberi tambahan newborn calf serum (NBCS) 10%, non essential amino acid (NEAA) 10%, NaHCO3, ITS 1 µl/ml (mengandung insulin 5 μg/ml, transferin 10 μg/ml, selenium 5 μg/ml; Sigma St Louis USA), dan 50 µg/ml gentamisin (mDMEM), dengan dan tanpa ekstrak Cissus quadrangula (CQ). Penelitian terdiri atas lima perlakuan yakni kontrol positif (mDMEM + deksametason 10-8 M), kontrol negatif (mDMEM), dan tiga konsentrasi CQ: mDMEM + CQ 0,3 mg/ml; mDMEM + CQ 0,6 mg/ml; dan mDMEM + CQ 1,2 mg/ml. Kultur dilakukan dalam inkubator CO2 5%, pada suhu 37° C selama tujuh hari. Parameter yang diamati adalah konsentrasi sel, komposisi, diameter osteoblas, dan diameter osteosit. Konsentrasi sel dihitung menggunakan hemositometer Newbauer. Komposisi osteoblas dan osteosit ditentukan berdasarkan pengamatan morfologi di bawah mikroskop cahaya. Diameter sel diukur menggunakan eyepiece micrometer. Data dianalisis menggunakan analisis varians dan uji Duncan. Hasil penelitian menunjukkan bahwa pemberian ekstrak Cissus quadrangula Salisb. pada konsentrasi 0,3 mg/ml; 0,6 mg/ml; dan 1,2 mg/ml secara signifikan dapat meningkatkan proliferasi sel tulang; dan pada konsentrasi 0,6 mg/ml mampu menginduksi diferensiasi osteoblas menjadi osteosit (P0,05). Disimpulkan bahwa pemberian ekstrak Cissus quadrangula pada konsentrasi 0,6 mg/ml ke dalam medium kultur dapat meningkatkan proliferasi dan diferensiasi osteoblas.

STUDI ANATOMIS DAN HISTOLOGIS PANKREAS BIAWAK AIR (Varanus salvator) (Anatomical and Histological Study of the Pancreas of Water Monitor Lizard (Varanus salvator)) Hamny Hamny; Muhammad Iqbal; Sri Wahyuni; Mustafa Sabri; M. Jalaluddin; Rinidar Rinidar
Jurnal Kedokteran Hewan Vol 10, No 2 (2016): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v10i2.5047

The aim of this research was to determine the anatomy and histology of pancreas of water monitor lizard (Varanus salvator). Two pancreas were used in this research (a male and a female). Water monitor lizard was euthanized using chloroform and subsequently necropsied to collect the pancreas. The pancreas was washed in 0.9 % NaCl solution. Anatomical observations were carried out including location, shape, and color of pancreas, also the length and weight measurements. Then, pancreas was fixed with 4% paraformaldehyde for 7 days to proceed to histological preparation and hematoxylin eosin (HE) staining. The results of the anatomical observations showed that pancreas of water monitor lizard have two lobes, dorsal lobe (splenic lobe) and ventral lobe (duodenal lobe). Dorsal lobe which was in oval form adhered with the spleen, and ventral lobe was located in the cranial of intestines with the form of long spherical. Both of pancreas lobes were white-yellow color with the length of 1.45Â±0.64 cm (dorsal lobe) and 7.00Â±0.42 cm (ventral lobe). Weight of dorsal lobe was 0.15Â±0.01 g and ventral lobe was 6.35Â±2.30 g. The histological observations found the acinar cells, ducts, and blood vessels in both of pancreas lobes. Additionally, the similar structure of Langerhans islet only found at the ventral lobe of pancreas. In conclusion, pancreas of water monitor lizard consists of two lobes which located differently. Ventral lobe is larger than the dorsal lobe. Both lobes have similar histological structure except the Langerhans islets are only appeared in the ventral lobe.

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