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THE IMPROVEMENT OF SEMEN QUALITY AND TESTOSTERONE LEVEL OF BALI CATTLE AFTER PROSTAGLANDIN F2α ADMINISTRATION Sari, Eka Meutia; Tanjung, Satria; Sari, Dewi Ratna; Akmal, Muslim; Siregar, Tongku Nizwan; Thasmi, Cut Nila
Jurnal Kedokteran Hewan Vol 13, No 4 (2019): December
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v13i4.14821

Abstract

This study aimed to determine the effect of prostaglandin F2α (PGF2α) administration on improving semen quality and testosterone levels on Bali cattle. This study used 3 Bali cattle aged 2, 3.5, and 5 years old. The sample used in this study was Bali cattle semen. In this study, the Latin Square Design was used with three different treatments were administered over three time periods. The treatments performed were P1 (5 mL physiological NaCl), P2 (25 mg prostaglandin), and P3 (37.5 mg prostaglandin) while treatment period was three weeks of treatment administration, which were 1st. 2nd, and 3rd week. Blood collected through coccygea vein 30 minutes after treatment to measure testosterone levels using enzyme-linked immunosorbent assay (ELISA) technique. Statistical analysis showed that PGF2α administration at a dose of 25 mg and PGF2α at a dose of 37.5 mg had no effect (P 0.05) on volume, color, pH, consistency, concentration, and motility of Bali cattle spermatozoa. The volume of semen obtained from P1; P2; and P3 were 6.5±0.9; 6.3±0.6; and 6.2±1.2 mL, respectively. The color of the semen in P1, P2, and P3 were beige and milky white. The pH in groups P1; P2; and P3 were 6.7±0.6; 7.1±0.2; and 6.5±0.2, respectively. Spermatozoa concentration in P1; P2; and P3 were 1,328±96.43 x 106 cells/mL; 1,354±102.19 x 106 cells/mL; and 1,353.3±88.55 x 106 cells/mL, respectively. Spermatozoa motility in P1; P2; and P3 were 73.3±2.8%; 71.6±2.8%; and 73.3±2.8%, respectively. Testosterone levels in P1 and P3 were 5.05±0.22 and 6.74±1.38 ng/mL, respectively (P 0.05). Based on the results of this study, it was concluded that the administration of PGF2α does not affect semen quality but can increase the level of testosterone on Bali cattle.
SUPLEMEN BUNGKIL INTI SAWIT TEPUNG DAUN KATUK BERPOTENSI MENINGKATKAN KUALITAS SPERMATOZOA PADA KAMBING PERANAKAN ETTAWA Akmal, Muslim; Reza Ferasyi, Teuku; Budiman, Hamdani; R, Razali; A, Azhari; A, Anwar; Aji Pamungkas, Fitra; Nasution, Saddat; Armansyah, T.; Hambal, Muhammad; S, Syafruddin; Sayuti, Arman
Jurnal Kedokteran Hewan Vol 8, No 2 (2014): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v8i2.2638

Abstract

Penelitian ini bertujuan mengetahui pengaruh pemberian suplemen bungkil inti sawit (BIS), tepung daun katuk (KAT), dan kombinasi bungkil inti sawit dan tepung daun katuk (BISKAT) terhadap peningkatan kualitas spermatozoa kambing jantan peranakan Ettawa (PE). Dalam penelitian ini digunakan 20 ekor kambing jantan PE, berumur 1,5 tahun dengan berat badan antara 15 -20 kg dan dibagi atas empat kelompok yakni P0, P1, P2, dan P3 yang masing-masing diberi akuades, BIS 100 g/hari/ekor, kombinasi BIS 100 g/hari/ekor dan KAT 15 g/hari/ekor, dan KAT 15 g/hari/ekor. Pemberian perlakuan dilakukan selama 35 hari. Pada hari ke-36 dilakukan kastrasi dan selanjutnya dilakukan pemeriksaan kualitas spermatozoa yang meliputi motilitas, viabilitas, integritas membran, dan abnormalitas. Hasil penelitian menunjukkan bahwa pemberian suplemen kombinasi BISKAT dapat meningkatkan motilitas, viabilitas, integritas membran, dan menurunkan abnormalitas spermatozoa dibanding kelompok kontrol. Disimpulkan bahwa pemberian suplemen kombinasi BISKAT berpotensi meningkatkan kualitas spermatozoa kambing PE.
CYCLOOXYGENASE-2 (COX-2) EXPRESSION ON TESTIS CONNECTIVE TISSUE OF Rattus norvegicusAFTER TREATMENT WITH BETEL NUT EXTRACT (Areca catechu) Akmal, Muslim; Riawan, Wibi
Jurnal Kedokteran Brawijaya Vol 23, No 3 (2007)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (824.806 KB) | DOI: 10.21776/ub.jkb.2007.023.03.3

Abstract

Betel nut contains alcaloids such as arecoline, arecaine, arecaidine, arecolidine, guvacine, guvacoline and isoguvasine. Arecoline has ability to change gonad morph-function, including shape abnormality of sperm. This research was conducted to prove the ability of betel nut extract (Areca catechu) in causing apoptosis on testis connective tissue of Rattus novergicus. This research used male; 2-3 months age, 150-200 grams body weight of white rats Rattus norvegicus strain Wistar. The rats were divided into 5 groups in equalnumber, 3 rats respectively. They were a control group without treatment and 4 groups as treatment groups which were given doses of betel nut extract, i,e., 1, 2, 3  and 4 gram during seven days. The result showed that dose variation ofbetel nut extract could induction of COX-2 expression on rats (Rattus norvegicus) strain Wistar testicular seminiferous tubule. Keywords: Betel nut extract, Rattus norvegicus, testis, cyclooxygenase-2
EPIDIDIMIS DAN PERANNYA PADA PEMATANGAN SPERMATOZOA Akmal, Muslim; Masyitah, Dian; Hafizuddin, Hafizuddin; Fitriani, Fitriani
Jurnal Edukasi dan Sains Biologi Vol 4, No 2 (2015): Jurnal Edukasi dan Sains Biologi
Publisher : Universitas Almuslim

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Epididymis is the important organ in male reproductive system. Functions of epididymis are as transportation, maturation, and storage of sperm. Sperm of testikular are non-functional gamets, and only during transit through the epididymis, the sperm will become functional gamets. Synthesis and secretion of some proteins by epididymis epithelium are very important for maturation of sperm in epididymis. The objective is to know the important role of epididymis in secretion of some proteins or molecule and its function for sperm maturation. Keywords : Epididymis, spermatogenesis, protein
HORMON DAN PERANNYA DALAM DINAMIKA FOLIKULER PADA HEWAN DOMESTIK ., Hafizuddin; Siregar, Tongku N; Akmal, Muslim
Jurnal Edukasi dan Sains Biologi Vol 1, No 1 (2012): Jurnal Edukasi dan Sains Biologi
Publisher : Universitas Almuslim

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Dinamika folikuler atau sering disebut dengan gelombang folikuler merupakan suatu perkembangan folikel (folikulogenesis) meliputi pertumbuhan serentak sekelompok folikel, satu diantaranya akan menjadi folikel dominan, mencapai ukuran terbesar, serta akan menekan perkembangan folikel-folikel lain. Folikulogenesis adalah proses yang bertanggung jawab untuk perkembangan folikel ovulatori dan pelepasan satu atau lebih oosit pada interval tertentu pada keseluruhan siklus reproduksi hewan betina. Jumlah gelombang per siklus tidak tergantung pada usia atau breed hewan. Gelombang folikel terdiri dari kelompok folikel yang mengalami perkembangan serentak selama fase akhir pertumbuhan. Tujuan penulisan ini adalah untuk mengetahui hormon apa saja yang terlibat dalam proses tersebut serta peranannya. Dinamika folikuler di negara maju sudah di aplikasikan dalam memantau fertilitas dan manipulasi reproduksi, sehingga dengan pengetahuan tersebut juga akan dapat menambah pengetahuan fungsi ovarium pada hewan.Kata kunci : hormon, dinamika folikuler, hewan domestic
6. Administration of Centella Leaf Extract (Centella asiatica (L.) Urban) for Decreasing cAMP Responsive Element Modulator (CREM) Expression in Testicular Seminiferous Tubule of Male Rats (Rattus norvegicus) Darmayanti, Susi; Wahyuni, Sri; Akmal, Muslim; Siregar, Tongku Nizwan; Sugito, Sugito
The International Journal of Tropical Veterinary and Biomedical Research Vol 1, No 2 (2016): Vol. 1 (2) November 2016
Publisher : The Faculty of Veterinary Medicine of Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (186.092 KB) | DOI: 10.21157/ijtvbr.v1i2.6689

Abstract

The objective of this study was to determine the effect of centella leaf extract administration on decreased of the molecule cAMP responsive element modulator (CREM) expression in the testicular seminiferous tubules of male rats (Rattus norvegicus). Eight rats, aged 3.5 months with 150-250 grams of body weight (BW) were used in this study. All rats were divided randomly into four groups as if K0 as a control group whereas K1, K2, and K3 were given the centella leaf extract with doses 125, 250, and 500 mg / kg body weight respectivelly that given once daily for 30 days. At the end of the treatment, rats were sacrificed and their testes were collected and subsequently fixed in buffered neutral formalin (BNF) 10% as fixative solution for histological preparation. The CREMs expressions were detected using immunohistochemical methods. The results showed that the number of CREM expression in the seminiferous tubules significantly differ (P 0.05) between K0 and the treatment group (K1, K2, and K3). Conclusion, the administration of centella leaf extract with of the dose 125, 250, and 500 mg/kg BW can decreased CREM expression spermatids of testicular seminiferous tubules in male rat.
The Administration of Epididymis Extract Increased the Testosterone Concentration without Affects the Dihydrotestosterone Concentration in Local Male Goat Yuliansyah, Nanda; Akmal, Muslim; Siregar, Tongku Nizwan; Wahyuni, Sri; Abrar, Mahdi; Syafruddin, Syafruddin; Gholib, Gholib; Athaillah, Farida
The International Journal of Tropical Veterinary and Biomedical Research Vol 2, No 2 (2017): Vol. 2 (2) November 2017
Publisher : The Faculty of Veterinary Medicine of Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (281.671 KB) | DOI: 10.21157/ijtvbr.v2i2.9469

Abstract

This study was aimed to determine the effect of epididymis extract (EE) on the testosterone and dihydrotestosterone (DHT) level of local male goat. An experimental study was performed using a completely randomized design (CRD) pattern of one-way analysis of variance (ANOVA). 15 local male goats aged 1.5 years with body weight 14-16 kg were used in this study. The K0 group as a control group, injected with only 1 ml physiological saline, while each KP1, KP2, KP3, and KP4 groups treated with multilevel EE dose, ie 1, 2, 3, and 4 ml / goat for 13 consecutive days. At the end of treatment (day 14th), testes, epididymis (caput, corpus, and cauda) and ductus deferens samples were taken through the close-castration method for examining the testosterone and DHT concentration by using enzyme-linked immunosorbent assay (ELISA) technique. Data gathered were later analyzed using ANOVA followed by Tukey’s HSD in SPSS 16.0 for Windows. The result showed that the average concentration of testosterone on K0, KP1, KP2, KP3, and KP 4 in testis respectively were 10.00±2.64 ng/ml; 7.66±2.51 ng/ml; 10.00±6.55 ng/ml; 0.66±0.57 ng/ml; 11.66±7.37 ng/ml; caput epididymis; 5.00±1.73 ng/ml; 2.33±1.52 ng/ml; 5.00±2.64 ng/ml; 1.33±0.57 ng/ml; 5.66±1.15 ng/ml; corpus epididymis; 1.33±0.57 ng/ml; 0.66±0.57 ng/ml; 4.00±2.64 ng/ml; 0.66±0.57 ng/ml; 4.33±2.30 ng/ml; cauda epididymis: 1.00±0.00 ng/ml; 0.66±0.57 ng/ml; 1.66±0.57 ng/ml; 1.00 ± 0.00 ng/ml; 2.00±1.73 ng/ml; ductus deferens: 3.66±2.51 ng/ml; 0.66±0.57 ng/ml; 3.00±1.00 ng/ml; 1.00±0.00 ng/ml and 3.66±1.15 ng/ml. While the average concentration of DHT on K0, KP1, KP2, KP3, and KP 4 in testis respectively; 10.00±2.64 ng/ml; 7.66±2.51 ng/ml; 10.00±6.55 ng/ml; 0.66±0.57 ng/ml; 11.66±7.37 ng/ml; caput epididymis; 5.00±1.73 ng/ml; 2.33±1.52 ng/ml; 5.00±2.64 ng/ml; 1.33±0.57 ng/ml; 5.66±1.15 ng/ml; corpus epididymis; 1.33±0.57 ng/ml; 0.66±0.57 ng/ml; 4.00±2.64 ng/ml; 0.66±0.57 ng/ml; 4.33±2.30 ng/ml; cauda epididymis: 1.00±0.00 ng/ml; 0.66±0.57 ng/ml; 1.66±0.57 ng/ml; 1.00 ± 0.00 ng/ml; 2.00±1.73 ng/ml; ductus deferens: 3.66±2.51 ng/ml; 0.66±0.57 ng/ml; 3.00±1.00 ng/ml; 1.00±0.00 ng/ml and 3.66±1.15 ng/ml. Statistical analysis showed that the administration of EE only increased testosterone concentration in testes had significant effect (P 0.05). From this study, it can be concluded that the EE has the potential to improve spermatogenesis and sperm quality through increasing the testosterone concentration in the local male goats.
INTEGRITAS MEMBRAN PLASMA SPERMATOZOA SAPI ACEH PASCA PEMBEKUAN DALAM MEDIA SITRAT KUNING TELUR DENGAN WAKTU EKUILIBRASI YANG BERBEDA (The Plasma Membrane Integrity Of Post-Freezing Aceh Cattle Spermatozoa In Yolk-Citrate Medium In Different Equilibration Times) hidayat, nikhofebri; dasrul, dasrul; hamdan, hamdan; husnurrizal, husnurrizal; akmal, muslim; murtina lubis, triva
JURNAL ILMIAH MAHASISWA VETERINER Vol 2, No 2 (2018): FEBRUARI - APRIL
Publisher : JURNAL ILMIAH MAHASISWA VETERINER

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (177.296 KB) | DOI: 10.21157/jim vet..v2i2.6927

Abstract

Penelitian ini bertujuan untuk mengetahui pengaruh waktu ekuilibrasi terhadap integritas membran plasma spermatozoa sapi aceh pasca pembekuan dengan media sitrat kuning telur. Sampel yang digunakan adalah semen sapi segar yang dikoleksi menggunakan vagina buatan. Sampel semen dikoleksi dari 1 ekor sapi aceh jantan sehat berumur 3 tahun sebanyak satu kali dalam seminggu selama lima minggu. Semen yang berkualitas baik diencerkan dengan pengencer sitrat kuning telur. Selanjutnya dibagi menjadi 5 kelompok perlakuan dengan waktu ekuilibrasi yang berbeda-beda  (K1); 2 jam (K2); 3 jam (K3); 4 jam (K4); 5 jam (K5); 6 jam dan masing-masing kelompok diulangi sebanyak 5 kali. Kemudian dilakukan pembekuan dengan medote vitrifikasi di atas uap nitrogen cair (suhu sekitar (-110 ºC) selama 13 menit  dan disimpan di dalam kontainer berisi nitrogen cair (-196ºC). Setelah penyimpanan selama 1 minggu, masing-masing semen beku dilakukan thawing dan selanjutnya dihitung persentase membran plasma utuh (MPU) setiap straw perlakuan. Data yang diperoleh dianalisis menggunakan analysis of variance (ANOVA) dan dilanjutkan dengan uji Duncan. Rata-rata MPU spermatozoa sapi aceh pada media sitrat kuning telur K1; K2; K3; K4 dan K5adalah 34,52±4,59%; 52,06±5,86%; 57,24±5,07%; 48,22±3,70% dan 5,44±1,07%. Hasil penelitian menunjukkan waktu ekuilibrasi berpengaruh nyata (P0,05) terhadap persentase MPU spermatozoa sapi aceh. Penggunaan waktu ekuilibrasi 4 jam menghasilkan MPU spermatozoa sapi aceh yang lebih baik dibandingkan lama waktu ekuilibrasi lainnya pasca pembekuan. (This study is aimed to determine the effect of equilibration time on the integrity of aceh cattle spermatozoa plasma membrane. The sample used was fresh cattle semen collected using artificial vagina. The semen samples were collected from one healthy cattle aged three years old once in a week during five weeks. Good quality semen was divided into five treatment groups with different equilibration times which are (K1); 2 hours (K2); 3 hours (K3); 4 hours (K4); 5 hours (K5); 6 hours and the collecting process was repeated five times for each group. Then, the samples were frozen by vitrification method on the top of liquid nitrogen vapor (ambient temperature (-110 ºC)) for 13 minutes and stored in containers of liquid nitrogen (-196ºC). After one week of storage, each frozen semen was thawed and the percentage of the intact plasma membrane (MPU) was calculated for each treatment of straw. The data obtained were analyzed by using analysis of variance (ANOVA) and continued by using Duncan test. The mean of MPU aceh cattle spermatozoa on citrate yolk medium  K1; K2; K3; K4 and K5 are 34,52±4,59%; 52,06±5,86%; 57,24±5,07%; 48,22±3,70% and 5,44±1,07%. The study results showed equilibration times have a real impact (P0,05) on the of MPU aceh cattle spermatozoa. The use of a 4-hour equilibration time resulted in a better percentage of MPU of aceh cattle spermatozoa compared to other post-freezing equilibration times.)
PENGARUH PEMBERIAN VITAMIN E TERHADAP KADAR SUPEROKSIDA DISMUTASE SERUM TIKUS PUTIH (Rattus norvegicus) DIABETES MELITUS (The Effect of Vitamin E to Superoxide Dismutase Serum Level in Diabetes Mellitus Induced White Rat (Rattus norvegicus) ) Putra, Regi; dasrul, dasrul; sugito, sugito; akmal, muslim; zuhrawati, zuhrawati; syafruddin, syafruddin
JURNAL ILMIAH MAHASISWA VETERINER Vol 2, No 2 (2018): FEBRUARI - APRIL
Publisher : JURNAL ILMIAH MAHASISWA VETERINER

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (245.089 KB) | DOI: 10.21157/jim vet..v2i2.6759

Abstract

ABSTRAKPenelitian ini bertujuan mengetahui pengaruh pemberian vitamin E terhadap kadar SOD serum tikus putih (Rattus norvegicus) diabetes melitus. Penelitian ini menggunakan tikus putih sebanyak 25 ekor dengan kriteria: sehat, bobot badan 150 – 200 gram; umur ± 3 – 4 bulan; dan jenis kelamin jantan. Rancangan yang digunakan adalah rancangan acak lengkap (RAL) pola searah dengan 5 kelompok perlakuan. Masing-masing kelompok perlakuan terdiri atas lima ekor tikus. Kelompok tikus non-diabetes (KN), kelompok tikus diabetes induksi aloksan (KP), kelompok perlakuan yaitu tikus diabetes induksi aloksan dan diberi vitamin E dosis 50 IU/kgbb/hari (P1), 100 IU/kgbb/hari (P2), dan 150 IU/kgbb/hari (P3). Perlakuan vitamin E diberikan selama 28 hari. Pada hari ke-29 setelah perlakuan, dilakukan koleksi serum untuk pengukuran kadar SOD serum dengan spektrofotometer. Data yang diperoleh dianalisis dengan ANOVA satu arah dan uji lanjut Duncan. Hasil penelitian menunjukkan kadar SOD serum adalah 58,00 ± 7,48 U/ml, 36,50 ± 4,56  U/ml (KP), 42,55 ± 5,89 U/ml (P1), 50,44 ± 5,32 U/ml (P2), dan 55,20 ± 4,84 U/ml (P3). Hasil penelitian menunjukkan bahwa vitamin E dapat meningkatan kadar SOD serum secara signifikan (P0,05). Pemberian  vitamin E dengan dosis 150 IU/kg BB/hari menghasilkan peningkatan kadar SOD serum terbaik pada tikus putih diabetes melitus. Kesimpulan dari penelitian ini adalah pemberian vitamin E dapat meningkatkan kadar SOD serum tikus putih diabetes melitus.ABSTRACT            This study aims to know the effect of vitamin E administration to SOD serum level in diabetes mellitus induced white rats (Rattus norvegicus). This study used 25 white rat in criteria: healthy; body weight 150 – 200 gram; age ± 3 – 4 month; and male sex. The research design was used completely randomized design (CRD) with 4 unidirectional pattern groups treatments. Each group consisted of five rat. Group of non-diabetic rats (KN), group of alloxan-induced diabetic rats (KP), group of alloxan-induced diabetic rats which given with vitamin E 50 IU/kgBW/day (P1), 100 IU/kgBW/day (P2), and 150 IU/kgBW/day (P3). Treatment of vitamin E was given in 28 days. On the 29th day after treatment, serum collection was performed to quantify SOD serum level by spectrophotometers. The data obtained were analyzed by one-way ANOVA and continued with Duncan test. The result showed SOD serum level was 58.00 ± 7.48 U/ml, 36.50 ± 4.56 U/ml (KP), 42.55 ± 5.89 U/ml (P1), 50.44 ± 5.32 U/ml (P2), and 55.20 ± 4.84 U/ml (P3). The result showed that therapy of vitamin E could increase SOD serum level significantly (P0.05). Treatment dose of 150 IU/kgBW/day was the best increasing of SOD serum level in diabetes mellitus induced white rat. The conclusion of research showed that giving of vitamin E can increasing the SOD serum level in diabetes mellitus induced white rat.
Gambaran histologis folikel ovarium sapi aceh pascavitrifikasi menggunakan etilen glikol Sayuti, Arman; Hidayah, Jamilatun; Akmal, Muslim; Panjaitan, Budianto
ARSHI Veterinary Letters Vol. 3 No. 1 (2019): ARSHI Veterinary Letters - Februari 2019
Publisher : Faculty of Veterinary Medicine, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (473.688 KB) | DOI: 10.29244/avl.3.1.3-4

Abstract

Penelitian ini bertujuan mengetahui gambaran histologis folikel ovarium sapi aceh pasca vitrifikasi menggunakan etilen glikol (EG) dengan berbagai konsentrasi berbeda yaitu 10%, 20% dan 30%. Phosphate buffered saline (PBS), 0.25 M sukrosa, 0.5 M sukrosa dan krioprotektan EG digunakan sebagai larutan vitrifikasi. Ovarium dipaparkan dengan larutan mengandung krioprotektan EG konsentrasi 10%, 20%, dan 30% selama 5 menit masing-masing pada suhu kamar, dikemas dalam straw, lalu divitrifikasi dalam nitrogen cair (-196 ⸰C), dan thawing dalam air pada 37 ⸰C. Kondisi normal dan keutuhan folikel pascavitrifikasi diamati secara histologi. Hasil pengamatan menunjukkan bahwa ovarium setelah vitrifikasi dengan EG 10% dan 20% memperlihatkan presentase keutuhan folikel 32.83% dan 45.04%, sedangkan EG 30% memperlihatkan presentase tertinggi yaitu 54.96%. Dari hasil penelitian ini dapat disimpulkan bahwa EG 30% lebih baik dalam mempertahankan keutuhan folikel ovarium sapi aceh dibandingkan dengan EG 10%  dan 20%.