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3-ACETYL -2,5,7-TRIHYDROXY-1,4-NAPHTALENEDIONE, AN ANTIMICROBIAL METABOLITE FROM THE CULTURE OF ENDOPHYTIC FUNGUS COELOMYCETES TCBP4 FROM Tinospora crispa Agusta, Andria; Nurkanto, Arif; Fathoni, Ahmad; Jamal, Yuliasri; Praptiwi, Praptiwi
Media Penelitian dan Pengembangan Kesehatan Vol 23, No 3 Sep (2013)
Publisher : Badan Penelitian dan Pengembangan Kesehatan

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Abstract

Abstract Isolation, identification and testing of antimicrobial activity of secondary metabolites of endophytic fungal culture TCBP4 isolated from bratawali (Tinospora crispa) has been performed. The fungus TCBP4 was cultivated in Potato Dextrose Broth (PDB) for 1 month, media and fungi extracted with ethyl acetate. The extract was separated and purified by several chromatographic techniques, from which 9 fractions were obtained. Fraction 3e was purified again and was obtained 6 fractions (3e1-3e6). Fractions 3e3-3e6 were tested against bacteria isolates Staphylococcus aureus Bacillus subtilis,Eschericia coli and yeast isolate Candida albicans by microdillution method. Antimicrobial activity test result showed that fractions 3e3-3e5 had better antibacterial activity compared to chloramphenicol as commercial antibiotic. It was indicated by MIC value of the fractions was lower (8 ug/ml) compared with the antibiotic chloramphenicol (16 ug/ml). Fraction 3e3 had better antifungal activity compared to commercial antifungal nystatin and cabisidin against C. albicans. GC-MS analysis showed that the chemical constituent of 3e5 fraction was identified as 3-acetyl -2,5,7-trihydroxy-1,4-naphtalenedione . Key words : Tinospora crispa, endophytic fungi, isolation, identification, antimicrobial Abstrak Isolasi, identifikasi dan uji aktivitas antimikrobia terhadap metabolit sekunder dari kultur jamur endofit TCBP4 yang diisolasi dari tumbuhan bratawali (Tinospora crispa). Jamur endofit TCBP4 dikultivasi pada media Potato Dextrose Broth (PDB) selama 1 bulan, selanjutnya media dan jamur diekstrak dengan pelarut etil asetat. Ekstrak dipisahkan dan dimurnikan dengan beberapa teknik kromatografi sehingga diperoleh 9 fraksi. Fraksi 3e dimurnikan kembali dan diperoleh 6 fraksi (3e1-3e6). Fraksi 3e3-3e6 diuji aktivitas antibakterinya terhadap beberapa isolate bakteri Staphylo-coccus aureus Bacillus subtilis, Eschericia coli dan isolate khamir Candida albicans dengan metode mikrodilusi. Hasil uji antimikrobia menunjukkan bahwa fraksi 3e3-3e5 mempunyai aktivitas antibakteri lebih baik bila dibandingkan dengan chloramfenikol, dimana nilai MIC dari fraksi (8 ug/ml) lebih rendah dari antibiotika chloramfenikol (16 ug/ml). Fraksi 3e3 mempunyai aktivitas antijamur lebih baik bila dibandingkan dengan antijamur komersial nistatin dan kabisidin terhadap  C. albicans. Analisis GC-MS menunjukkan bahwa komponen kimia dari fraksi 3e5 diidentifikasi sebagai 3-acetyl -2,5,7-trihydroxy-1,4-naphtalenedione . Kata kunci : Tinospora crispa, jamur endofit, isolasi, identifikasi, antimikrobia
CHEMICAL CONSTITUENTS AND ANTIBACTERIAL EFFECT OF ESSENTIAL OIL OF JAVANEESE PEPPER LEAVES (PIPER RETROFRACTUM VAHL.) Agusta, Andria; Fathoni, Ahmad; Irawati, Pipit; Jamal, Yuliasri
Media Penelitian dan Pengembangan Kesehatan Vol 23, No 2 Jun (2013)
Publisher : Badan Penelitian dan Pengembangan Kesehatan

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Abstract

Abstrak Daun cabe jawa (Piper retrofractum Vahl.) telah terbukti berkhasiat sebagai bahan antimikroba, akan tetapi belum ada informasi lebih lanjut tentang mekanisme aksi antibakteri dari minyak atsiri daun cabe jawa. Penelitian ini untuk mengetahui pengaruh minyak atsiri daun cabe jawa terhadap bakteri uji sensitif serta difokuskan untuk melihat kerusakan membran sel bakteri melalui analisis protein, asam nukleat dan ion-ion logam kalsium dan kalium. Distilasi uap minyak atsiri dari daun segar cabe jawa (Piper retrofractum Vahl.) menghasilkan 0.03% cairan kental kekuningan. Analisis dengan GC-MS mengidentifikasi 4 senyawa sebagai komponen utama, yaitu germakren D (24.20%), tetrametilsiklo[5.3.1.0(4.11)]-undek-8-ena (17.73%), ar-turmeron (11.55%) dan benzil benzoat (6.28%). Minyak atsiri cabe jawa terbukti aktif melawan beberapa bakteri patogen antara lain Staphylococcus aureus, Bacillus subtilis and Micrococcus luteus dengan diameter daya hambat berturut-turut 8.0; 9.7; 8.5 mm. Pengamatan lebih lanjut memperlihatkan nilai MIC dari minyak atsiri cabe jawa  terhadap B. subtilis adalah 2% (v/v), dan juga merubah morfologi sel dari bakteri yang diobservasi menggunakan SEM. Kata kunci: Piper retrofractum Vah., cabe jawa, minyak atsiri, aktivitas antibakteri Abstract The leaves of Javaneese pepper (Piper retrofractum Vahl.) has been proven efficacious as antimicrobial agent, however, there was no further information about action mechanism of antibacterial of Javaneese pepper leaves essential oil. This study was to determine the effect of essential oils of javaneese pepper leaves against bacterial sensitive test and is focused on investigating the damage of cell membranes of bacteria through the analysis of proteins, nucleic acids and metal ions, calcium and potassium. Steam distillation of essential oil from fresh leaves of Javaneese pepper (Piper retrofractum Vahl.) gave 0.03 % yellowish viscous liquid. Germacrene D (24.20 %), tetramethylcyclo[5.3.1.0(4.11)]-undec-8-ene (17.73 %), Ar-turmeron (11.55 %) and benzyl benzoate (6.28 %) were identified as major constituents analyzed by GC-MS. Javaneese pepper essential oil was active against pathogenic bacteria Staphylococcus aureus, Bacillus subtilis and Micrococcus luteus with diameter of clear zone of 8.0, 9.7 and 8.5 mm respectively. Further investigation showed MIC value of the oil against B. subtilis was 2 % (v/v), and was also alter the cell morphology of tested bacterium observed by SEM. Key words: Piper retrofractum Vahl., Javeneese pepper, essential oil, antibacterial activity
KOMPOSISI MINYAK ATSIRI DARI TIGA JENIS TUMBUHAN RUTACEAE [ Composition of Essential Oils from Three Rutaceae Species Plant] Jamal, Yuliasri; Agusta, Andria
BERITA BIOLOGI Vol 4, No 5 (1999)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (527.358 KB) | DOI: 10.14203/beritabiologi.v4i5.1251

Abstract

Rutaceae is one of the essential oil resources that is very potential for medicinal, perfumery and other use This study was conducted to analyze the essential oil components resulted from water distillation of kalamanjarik leaves (Micromelum minutum), jeruk epara leaves ILimnocitrus litoralis) and the leaves and the stem bark of malapotung (Evodia qlabra). The results showed the leaves of kalamanjarik contained approximately 0,70%, jeruk jepara 0,21%, malapotung leaves 0,17% and the stem bark contained 0,50 % essential oils. GCMS analyses of the essential oils gave 3 major components of kalamanjarik leaves i.e /J-pinene (24,41%j, bergamiol (23,01%) and nerolidol (18,90%) and 2 major components of jeruk jepara leaves i.e fl-mircene (70.55) and /t-pinene (24,06 %). Essential oil of malapotung leaves consisted of 39,8J% ct-pinene, 14,26 % 1aR(1aa,4a/3,7a,7aa,7ba)decahydro -1,1,7-lrimethyl-4-methylene-1H-cycloprope azulene and 16,62% S-cadinene as major components, while the stem bark had only one major component, u-pinene (68,37 %) The minor components of the four essential oils will be discussed.
ANALISIS KOMPONEN KIMIA DAUN WATI {PIPER METHYSTICUM FORST. F) Chairul, Chairul; Jamal, Yuliasri; Agusta, Andria
BERITA BIOLOGI Vol 4, No 2&3 (1998)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v4i2&3.1276

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Wati(Piper methysticum Forst.f.)leaves has been used as narcotic in some parts of Irian Jaya since longtime ago. The GCMS analysis of wati leaves organic extracts(hexane,chloroform, methanol)has detected 48 components consisted alkanes,oxygenated hydrocarbons, fatty acid, steroids and alkaloids,including 5 major components, i.e. dihydro kawain,anisol p-undecyl, 2,3-dihydro-3,5-dihydroxy-6-methyl-4H-pyran-4-on, levulinic acid and one of unknown component.Forty-three other minor components will be discussed.
Produksi Metabolit Utama (-)-Citrinin, pada Kultur Jamur Endofit Penicillium sp dari Tanaman Teh Agusta, Andria; Jamal, Yuliasri
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 13, No 3 (2008): October 2008
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (104.756 KB) | DOI: 10.24002/biota.v13i3.2570

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Endophytic fungi have been recognized as source of broad range biological active metabolites with high chemical structure diversity. The purpose of this research is to isolate and characterize major metabolite produce by the endophytic fungi Penicillium sp isolated from a tea plant. Cultivation of endophytic fungus Penicillium sp (AB2245443) in liquid medium PDB on a rotary shaker at 100 rpm, temperature 25 – 30oC for 7 days, produced a yellow metabolite. Separation of metabolite through chromatography technique and followed by chemical structure elucidation based on MS, IR, NMR spectra and published data showed that the yellow metabolite is (-)-citrinin.
PENGARUH PENGERINGAN TERHADAP KANDUNGAN LEMAK CABE BESAR (CAPSICUM ANNUUM L.) PANGGABEAN, G.; JAMAL, YULIASRI
BERITA BIOLOGI Vol 3, No 8 (1988)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v3i8.1303

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YULIASRI JAMAL&G. PANGGABEAN. 1988 The effect of drying on the fat content of th chillies (Capsicum annuum L.). Berita Biologi 3(8) 374 - 376. The treating of chillies on various temperatures were studied in order to know their effect on the fat content.The chillies were dried in the refrigerator (12°C),in the room at ambient temperature (25°C ±2°C), in the sun shine (35°C ± 2°C) in the oven at various temperatures such as 40°C 50°C, 60°C, 70°C, 80°C, 90°C and 100°C.During the investigation were used cabe keriting and cabe besar biasa & it were obtained from the local market.The results of the observation that the highest fat content were found on the cabe keriting a 70 C drying as well as room temperature.The fat content of terated chillies, however, in the oven at 50°C, 60°C were similar 14%.Meanwhile,the dried chillies as the result on the sun shine was 3,5% fat content. The fat content of cabe besar biasa was significantly high at 70°C. The fat content of the chillies decreased rapidly on the treating in the sun shine.The chillies both cabe keriting and cabe besar biasa which were dried more than 60°C & in the sun shine, the pulp turned to brownish red, whereas the dried chillies in the refrigerator, at room temperature, 40°C, 50°C and 60°C the colour of the pulp were similar to the fresh fruits.
MINYAK ATSIRI Cinnamomum celebicum L. SEBAGAI SUMBER ISOEUGENOL YANG POTENSIAL Jamal, Yuliasri
BERITA BIOLOGI Vol 6, No 4 (2003)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2215.021 KB) | DOI: 10.14203/beritabiologi.v6i4.3459

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EFEK HYPOGLIKEMIK EKSTRAK ALKOHOL HERBA MENIRAN (Phyllanthus niruri L.) PADA KELINCI PUTIH JANTAN Zainul, Zuherti; Jamal, Yuliasri; Chairul, Chairul
BERITA BIOLOGI Vol 5, No 1 (2000)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (456.884 KB) | DOI: 10.14203/beritabiologi.v5i1.1103

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A study on the influences of alcohol extract of Meniran or Phyllanthus niruri L. (Euphorbiaceae) to blood glucose concentration in rabbits (Oxytolagus cuniculus) had been carried out using glucose tolerance method.The extract was administered orally at 10, 20, 30, 40 and 50 mg/kg BW.Prior to tfie extract administration, all rabbits were put on hyperglycemic condition by injecting glucose solution intravenally at dose of 0.5 g/kg BW.The blood glucose concentration was measured every one hour starting at 2 hours before and until 7 hours after administration. Antidiabetic drug,tolbutamid 250 mg/kgBW.was used as control.The results indicated that extract with dose at 30 mg/kg BW gave a hypoglycemic effect similar to control (Tolbutamid). The blood glucose decreased regularly as the extract dose being higher.Using statistical analyses showed significant differences among the groups, especially after the 2 and 3 hours of treatment (P<0.05).
DIVERSITAS DAN PROFIL METABOLIT SEKUNDER JAMUR ENDOFIT YANG DIISOLASI DARI TUMBUHAN GAMBIR (Uncaria gambler) SERTA AKTIVITAS BIOLOGISNYA SEBAGAI ANTIBAKTERI Kanti, Atit; Ilyas, Muhamad; Jamal, Yuliasri; Agusta, Andria
BERITA BIOLOGI Vol 9, No 2 (2008)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (853.072 KB) | DOI: 10.14203/beritabiologi.v9i2.2023

Abstract

Five kinds of endophytic filamentous fungi i.e. Pestalotiopsis sp. GNC, Fusarium sp. GNC-A, Fusahum sp. GNC-B, Pestalotiopsis sp. GUC and Fusarium sp. GUC were isolated from surface sterilized of steems of gambir nasi and gambir udang (Uncaria gambier Roxb.) collected from Rengat, Riau Province,Indonesia. Identification of the endophytic fungi were done through morphologically observations and chemotaxonomically approach. In an antibacterial assays, the ethyl acetate extract of the Pestalotiopsis sp.GNC cultures showed their activity against pathogenic bacteria, Eschericia coli, Staphylococcus aureus and Bacillus sublilis.Beside the above three pathogenic bacteria, the ethyl acetate extract of Fusarium sp. GNC-B culture was also active against
KOMPOSISI KIMIA MINYAK ATSIRI Melodorum cylindricum (Maing. ex Hook.f & 1 Thorns), Litseajirma (Blume) Hook.f., Fl. Brit. Ind. DAN Callistemon lanceolatus D.C. Jamal, Yuliasri
BERITA BIOLOGI Vol 9, No 6 (2009)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (726.806 KB) | DOI: 10.14203/beritabiologi.v9i6.848

Abstract

Steam distillation of Melodorum cylindricum leaves (176 gram), Litsea firma leaves (295 gram), L firma stem bark (1,590 gram) and Callistemon lanceolatus leaves (300 gram) yielded 0.15%, 1.68%, 0.23% and 0.4% essential oils respectively.The oils were analyzed using GC-MS methods. The results identified 71 components from the leaves of M. Cylindricum which p-myrcene is the highest (12,56%). Leaves and stem bark of L. firma contained 20 and 24 components which 2-undecanone (34,95%) and undecanone (29,57%) as the highest major components. The essential oil of C. lanceolatus identified 24 components and 1,8 cineol is the major component with the highest content, 67.37%.