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All Journal HAYATI Journal of Biosciences METAMORFOSA Journal of Biological Sciences Jurnal Veteriner Jurnal Biologi Udayana Bumi Lestari SIMBIOSIS Biogenesis: Jurnal Ilmiah Biologi KUKILA Advances in Tropical Biodiversity and Environmental Sciences
SANG KETUT SUDIRGA
Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Udayana
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Energy Environmental Science Immunology & microbiology Veterinary Other

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Birds of Nusa Dua Sewage Treatment Ponds, Bali, and an unusual foraging behaviour of egrets Dalem, Anak A.G.R; Sudirga, Sang K; Burgin, Shelly
KUKILA Vol 15 (2011)
Publisher : KUKILA

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Abstract

Y-Chromosome Microsatellite DNA Variations on Terunyan Bali Mula Society I KETUT JUNITHA; SANG KETUT SUDIRGA
HAYATI Journal of Biosciences Vol. 14 No. 2 (2007): June 2007
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (62.564 KB) | DOI: 10.4308/hjb.14.2.59

Abstract

The Balinese is comprised of genealogical patrilineal clans named as soroh. However, remnants of ancient Bali Mula communities have their own local genealogy which differ from the general soroh. The Pasek Trunyan together with Pasek Kayu Selem, Pasek Celagi, and Pasek Kayuan are part of Bali Mula which believed to be descendants of one ancestor, Empu Kamareka. Here we reported genetic variation of the Terunyan society. The microsatellites Y-chromosome markers (DYS19, DYS390, DYS393, and DYS395) were used in this study. There were 12 alleles found and the result showed the low genetic diversity (D = 0.28 + 0.05) within Terunyan society. Nine haplotypes based on allelic combination were found, dominated by major haplotype with frequency of 0.50 which spread out in many genealogical lineage called dadia. The haplotype data showed that Terunyan society derived from many gene sources. Key words: patrilineal, pasek, soroh, dadia, haplotipe
Isolation and molecular identification of fungi causing stem rot disease in Bali's local legumes Ni Made Susun Parwanayoni; Dewa Ngurah Suprapta; Nyoman Darsini; Sang Ketut Sudirga
Biogenesis: Jurnal Ilmiah Biologi Vol 9 No 1 (2021)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v9i1.20426

Abstract

Efforts to improve food security in Indonesia, particularly Bali, need to be supported by improvements in cultivation techniques, including the management of pests and diseases. Meanwhile, legume crops are often attacked by stem rot diseases which potentially decrease production and leads to economic losses. This disease is generally caused by the soil-borne pathogenic fungus Sclerotium rolfsii or Athelia rolfsii. The macroscopic and microscopic morphologies of these two species are the same and difficult to distinguish, hence, molecular identification is needed to differentiate between the species. Therefore, this study aims to isolate and molecularly identify the fungi causing stem rot disease in local legume plants in Bali. The methods used include isolation of pathogenic fungi from legumes showing symptoms of stem rot disease, pathogenicity test, identification of isolates with the highest virulent levels, DNA extraction, DNA amplification by PCR and electrophoresis, ITS region sequencing and computer analysis sequences. The isolation procedure identified six fungal isolates coded SKT, SKB1, SKB2 SKB3, SKL and SKN isolates. SKT isolates had the highest virulence rate as indicated by the pathogenicity test of peanut plants. Furthermore, molecular identification results show that SKT isolate is Athelia rolfsii, a similar clade with the fungi sequences in GenBank with 100% bootstrap support.
Birds of Nusa Dua Sewage Treatment Ponds, Bali, and an unusual foraging behaviour of egrets Anak A.G.R Dalem; Sang K Sudirga; Shelly Burgin
KUKILA Vol. 15 (2011)
Publisher : Indonesian Ornithologists’ Union

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Uji Antioksidan Ekstrak Daun Tanaman Leunca (Solanum nigrum L.) Affrina Fauziah; Sang Ketut Sudirga; Ni Made Susun Parwanayoni
Metamorfosa: Journal of Biological Sciences Vol 8 No 1 (2021)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2021.v08.i01.p03

Abstract

Leunca plant (Solanum nigrum L.) is one of the plants that is often used as food for the people of Indonesia. Leunca plant leaves are widely used for herbal medicines. This study aims to determine the phytochemical content and antioxidant activity of leunca leaf extract (Solanum nigrum L.). The study was conducted at the Analytical Laboratory of Udayana University, the Organic Laboratory of Chemistry Study Programs at Udayana University and the Laboratory of the Faculty of Agriculture of Udayana University. Phytochemical tests were carried out to determine the presence of alkaloid compounds, steroids / triterpenoids, flavonoids, phenols and tannins. The antioxidant activity of leunca leaf extract (Solanum nigrum Linn) was tested by DPPH method (1-1-diphenyl-2-picrilhidrazil), with 5 different concentrations and consisted of 4 replications. Leunca positive leaf extract contains alkaloids, steroids / triterpenoids, flavonoids, phenols and tannins. The results showed that dried leunca leaf extract and fresh leunca leaf extract had different antioxidant activity. Dry leunca leaf extract is classified as strong antioxidant with antioxidant activity value of 64.40%, IC50 value of 95.12 ppm and fresh leunca leaf extract is classified as weak antioxidant with antioxidant activity value of 16.83%, IC50 value of 243.66 ppm. Keywords: Solanum nigrum L., herb, phytochemicals, antioxidant, DPPH
Pengaruh Ekstrak Bawang Merah (Allium cepa L.), Air Kelapa (Cocos nucifera L.), Dan Metode Skarifikasi Terhadap Pertumbuhan Cendana (Santalum album L.) Barnadus Nggahu Lindi Djawa; Ni Luh Arpiwi; Sang Ketut Sudirga
Metamorfosa: Journal of Biological Sciences Vol 7 No 1 (2020)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2020.v07.i01.p09

Abstract

Tujuan penelitian ini untuk mengetahui pengaruh ekstrak bawang merah, air kelapa dan skarifikasi terhadap pertumbuhan cendana. Manfaat dari penilitian ini untuk menjawab permasalahan pembudidyaan tanaman cendana di Pulau Sumba. Penelitian ini mengunakan rancangan acak lengkap dengan enam perlakuan dan tiga kali ulangan pada masing-masing unit percobaan. Parameter yang diukur dalam penelitian ini persentase bibit tumbuh, tinggi tanaman, jumlah daun, panjang daun, dan lebar daun. Analisis data mengunakan ANOVA dengan taraf uji 5% bila terdapat beda nyata pada salah satu faktor dilanjutkan dengan uji Duncan Multiple Range Test (DMRT) pada taraf uji 5% menggunakan analisis statistik SPSS. Hasil penelitian menujukkan perlakuan ekstrak bawang merah dan metode skarifikasi memberikan hasil yang paling tinggi persentase bibit tumbuh sebesar 70%. Perlakuan kombinasi skarifikasi dengan air kelapa muda memberikan hasil yang paling tinggi terhadap pertumbuhan tanaman cendana. Untuk itu penulis merekomendasikan kepada petani cendana menggunakan ekstrak bawang merah atau skarifikasi untuk meningkatkan persentase bibit tumbuh sedangkan untuk memacu pertubuhan tanaman cendana menggunakan kombinasi skarifikasi dengan air kelapa muda. Kata kunci: bawang merah, air kelapa, skarifikasi
Aktivitas Antioksidan Ekstrak Kulit Jeruk Keprok (Citrus reticulata Blanco) So’e Sebagai Teh Tradisional Deglory Tunmuni; Ni Putu Adriani Astiti; Sang Ketut Sudirga
Metamorfosa: Journal of Biological Sciences Vol 8 No 2 (2021)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2021.v08.i02.p11

Abstract

The Citrus So'e plant is one of the leading community plants of the people of East Nusa Tenggara (NTT). The So'e tangerine is used as a raw material in the manufacture of orange juice and the peel has not been used. This research was conducted to utilize So'e tangerine peel as a traditional tea. The parameters observed included the active compound group, antioxidant activity, the type of active compound for So'e tangerine peel and the public's perception of the traditional So'e tangerine peel tea. The research method was carried out by qualitative phytochemical tests, test for the content of flavonoids, phenolics, and tannins by spectaphotometry, antioxidant tests using the DPPH method, testing for the types of active compounds using GC-MS and organoleptic tests to determine the level of consumer preference. The results showed that So'e tangerine peel extract had active compounds, namely flavonoids, phenolics, tannins, steroids, triterpenoids and alkaloids and So'e tangerine peel extract contained flavonoids = 253.901 mg / 100g QE, phenolic = 61.6099 mg / 100g GAE, tannin = 60.0496 mg / 100g TAE which has the potential as an antioxidant with an IC50 value of 81.811 mg / L which is included in the strong antioxidant category, and has various types of active compounds, namely the nonpolar fraction contains 53 compounds and the polar fraction contains 46 compounds activity and the level of public preference for So'e tangerine peel tea was higher in refined tea than coarse tea. Keywords: Citrus reticulata, phytochemicals, DPPH, GC-MS, traditional tea.
Isolasi Dan Identifikasi Senyawa Antijamur Daun Jeringau (Acorus calamus Linn.) Sebagai Pengendali Jamur Athelia rolfsii Sacc. Penyebab Penyakit Busuk Batang Pada Tanaman Kedelai Susun Susun Prawanayoni; Sang Ketut Sudirga
Metamorfosa: Journal of Biological Sciences Vol 7 No 2 (2020)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2020.v07.i02.p02

Abstract

The study was carried out to isolate and identify active compounds in jeringau leaf extract (Acorus calamus) and test their activity to inhibit the growth of Athelia rolfsii fungus that causes stem rot in soybean plants. The study began with extraction of Jeringau leaves, extract inhibition test on Athelia rolfsii fungus, partition, fractionation with column chromatography and thin layer chromatography (TLC), fraction inhibition test and identification of active compounds by Gas Chromatography-Mass Spectroscopy (GC-MS) analysis. Coarse jeringau leaf extract can inhibit A. rolfsii fungus with inhibition zone diameter of 25 mm. The results of the analysis of active fractions of jeringau extract using GC-MS (GCMS-QP2010 Ultra SHIMADZU) obtained 24 active compounds namely: Ethanol, 2-(2-ethoxyethoxy)-CAS; 6-Okthenal,3,7-dimethyl; beta-Citronellol; Trans-Geraniol; 6-Octen-1-ol 3,7-dimethil-acetate; Benzene 1-methoxy-4-(2-propenil); 2,6-Otahen-1-ol 3,7-dimethil-acetate; Caryophyllen; 3-Allyl-6- methoxyphenol; Dodecanoic acid methil ester; Methil tetradecanoate; Cis-Asarone; Alpha hexylcinnamic aldehyde; Hexadecanoic acid methyl esteter; 7-Acethyl-6-ethyl-1-1-4-4-tetramethylhetralin; benzyl benzoate; (2(Chloro-phenyl)-(2-Methoxy-6-MI; 9-Octadecenoic acid methyl ester; 9.12-Octadecadienoic acid (Z.Z)-methyl ester; Oxinaneoctanoic acid 3-octyl-methyl ester cis:; Octadecenoic acid 9.10-didydroxyl-methyl ester; Oxinaneoctanoic acid 3-octyl-methyl trans; 9-Octadecenoic acid (Z)-methyl ester (CAS); 1.2-Benzenedicarboxylic acid, bis (2-ethylhexy). Keywords: Extract, isolation, identification, jeringau, Athelia rolfsii
ISOLASI DAN IDENTIFIKASI JAMUR Colletotrichum spp. ISOLAT PCS PENYEBAB PENYAKIT ANTRAKNOSA PADA BUAH CABAI BESAR (Capsicum annuum L.) DI BALI Sang Ketut Sudirga
Metamorfosa: Journal of Biological Sciences Vol 3 No 1 (2016)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2016.v03.i01.p04

Abstract

A research on the isolation and identification of fungi Colletotrichum spp. PCS isolate as the cause of anthracnose desease on chili fruit (Capsicum annuum L.) in Bali. Fungi Colletotrichum spp. isolated from large chilies anthracnose symptoms were taken from three locations of chili planting in Bali i.e. Apuan Village, Tabanan (isolates APN), Village Belok Sidan, Badung (isolates BLS) and Village Pancasari, Buleleng (isolates PCS). To ensure that these three isolates of the fungus caused anthracnose on chili fruit, tested Koch's postulates and virulence test were used. Based on fungal virulence test, isolates PCS was the most virulent isolates than APN and BLS isolates, therefore the PCS isolates was further identified. Macroscopic  and microscopic identification of the fungus Colletotrichum spp. isolate PCS were conducted at the Laboratory of Biopesticides, Faculty of Agriculture, University of Udayana by observing the shape of the colony, colony color, growth rate, hyphae structure, spore shape, and size of the spores. The identification of the fungus Colletotrichum spp. isolate PCS was carried out in the Department of Molecular Biology, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University based on the analysis of 18S rRNA genes by using ITS1 and ITS4 primers. Phylogeny analyzes were performed using the MEGA 6.0, maximum parsimony method (MP) with 1000x bootstrap. The results showed that the fungus Colletotrichum spp. isolate PCS was a fungal of Colletotrichum acutatum as a clade with sequences of fungus C. acutatum by 100% of Bootstrap Support (BS). 
UJI AKTIVITAS EKSTRAK BIJI KAPUK RANDU (Ceiba pentandra Gaertn.) TERHADAP PERILAKU KAWIN TIKUS (Rattus norvegicus) JANTAN Dessy Sukma Wirastuti; Ngurah Intan Wiratmini; Sang Ketut Sudirga
Metamorfosa: Journal of Biological Sciences Vol 5 No 1 (2018)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2018.v05.i01.p02

Abstract

Kapuk randu (Ceiba pentandra Gaertn.) merupakan salah satu tanaman yang memiliki banyak manfaat untuk masyarakat. Tanaman ini dimanfaatkan sebagai penahan tanah dari erosi, tanaman peneduh, bahan pengisi kasur dan pakan ternak. Saat ini biji kapuk randu banyak dikembangkan sebagai bahan obat kontrasepsi herbal. Tujuan penelitian ini adalah untuk mengetahui aktivitas ekstrak biji kapuk randu (Ceiba pentandra Gaertn.) terhadap perilaku kawin tikus (Rattus norvegicus) jantan. Rancangan yang digunakan adalah Rancangan Acak Lengkap (RAL) dengan 4 kelompok perlakuan dan 8 ulangan yaitu P0 (aquades dan tween 80), P1 (0,07 mg/kg BB ekstrak biji kapuk randu), P2 (0,12 mg/kg BB ekstrak biji kapuk randu), P3(0,24 mg/kg BB ekstrak biji kapuk randu). Pemberian perlakuan ekstrak biji kapuk dilakukan selama 33 hari dengan pengamatan perilaku kawin dilakukan 3 hari sekali. Parameter yang diamati untuk menilai adanya perilaku kawin adalah jumlah kissing vagina, jumlah mount jumlah intromission. Hasil penelitian menunjukkan bahwa ekstrak biji kapuk randu memberikan pengaruh yang nyata terhadap perilaku kawin tikus jantan yang ditunjukkan dengan menurunnya jumlah kissing vagina, jumlah mount dan jumlah intromission tikus jantan kelompok perlakuan.
Co-Authors A.A.S. A. Sukmaningsih Affrina Fauziah Anak A.G.R Dalem Anak Agung Gde Raka Dalem Anak Agung Ketut Darmadi Ariyanti Ni Kadek Barnadus Nggahu Lindi Djawa Candra Dewi Oktaviawati Deglory Tunmuni Dessy Sukma Wirastuti Dessy Sukma Wirastuti Dewa Ngurah Suprapta Fitria Dwijayanti I Gusti Ayu Sugi Wahyuni I Ketut Ginantra I Ketut Junitha I KETUT MUKSIN I P. G. Ardhana I.G.N. Bayu Wirayudha IDA AYU ASTARINI Ida Bagus Gede Darmayasa Ida Bagus Made Suaskara Iriani Setyawati Junita Hardini Kadek Mardika Kadek Wegi Kurnilia MADE SUSUN PARWANAYONI Margareta Dacosta Muhammad Fathur Rozi Muhammad Inas Riandi Ngurah Intan Wiratmini Ni Desak Made Aprilia Dewi Ni LUH ARPIWI Ni Made Gari Ni Made Rai Suarni Ni Putu Adriani Astiti Ni Wayan Sudatri Nur Assiam Nyoman Darsini Retno Kawuri Shelly Burgin Silviana Febrionita Meiskia Agung Susun Susun Prawanayoni VEMY CLAUDIA Wafiatul Fitriyah Yan Ramona