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All Journal International Journal of Biosciences and Biotechnology METAMORFOSA Journal of Biological Sciences SIMBIOSIS Jurnal Mikologi Indonesia

Junita Hardini

Program Studi Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Udayana. Jl. Raya Kampus UNUD, Jimbaran, Kuta Selatan, 80361, Bali, Indonesia.

Author-ID : 2925726

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology

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EFFECTIVENESS OF Trichoderma asperellum TKD WITH MEDIATOR OF MANURE TO CONTROL FUSARIUM DISEASE ON RED PEPPER PLANT (Capsicum annum L.) Nyoman Mega Antari; Ida Bagus Gede Darmayasa; Junita Hardini
SIMBIOSIS Vol 8 No 2 (2020)
Publisher : Jurusan Biologi Universitas Udayana

Red pepper is agricultural products in Indonesia which is often attacked by fusarium wilt disease due to the fungus Fusarium oxysporum f.sp. capsici, so in this study aims to control of fusarium wilt disease using Trichoderma asperellum TKD with mediator of manure. Method in this study is RAL (Completely Randomized Design) with 7 treatments P1 = Control I (without treatment); P2 = Control II (F.oxysporum f.sp. capsici (disease)); P3 = disease + T. asperellum TKD 10 mL; P4 = disease + T. asperellum TKD 20 mL; P5 = disease + T.asperellum TKD 30 mL; P6 = disease + T. asperellum TKD 40 mL; P7 = disease + T. asperellum TKD 50 mL. The results showed that T.asperellum TKD was able to inhibit F.oxysporum f.sp. capsici in in vitro by 100%. The highest percentage of fusarium wilt disease (100%) is shown in plants that are only given F.oxysporum f.sp. capsici and without T. asperellum TKD, as well as plant height, total of fresh and dried leaves in plants added by T. asperellum TKD is better compared to plants that are not given T. asperellum TKD. The best plant height, number of freh leaves, and lowest number of dry leaves found in plants with 50 mL of T.asperellum TKD. Keywords: biocontrol, pathogenic fungi, Trichoderma asperellum TKD

DAYA HAMBAT EKSTRAK LENGKUAS PUTIH (Alpinia galanga L.) TERHADAP PERTUMBUHAN Escherichia coli O157:H7 dan Staphylococcus epidermidis Deny Christine Sidabutar; Ida Bagus Gede Darmayasa; Junita Hardini
SIMBIOSIS Vol 10 No 2 (2022)
Publisher : Jurusan Biologi Universitas Udayana

Escherichia coli O157:H7 and Staphylocccus epidermidis are bacteria that can cause disease in humans. Alternative control efforts are very important because the use of antibiotics can cause adverse side effects. This study aims to obtain extracts of white galangal (Alpinia galanga L.) in inhibiting the growth of E. coli O157:H7 and S. epidermidis. In this test, white galangal extract was macerated with three solvents at different polarity levels (96% ethanol, n-hexane and ethyl acetate). The ability of white galangal extract in inhibiting bacteria was tested using the Kirby Bauer method (paper disc). The concentration of galangal extract tested was 0%; 5% ; 10% ; 15%; 20% (w/v), control (ciprofloxacin) and MIC (Minimum Inhibition Concentration). To determine the toxicity of the extract, the LC50 test was carried out. Meanwhile, to determine the class of compounds contained in the galangal extract, phytochemical tests were carried out using the phytochemical screening method. The results showed that white galangal extract with 96% ethanol as solvent and n-hexane was able to inhibit the test bacteria. Inhibition against E. coli O157:H7 was most effective in n-hexane solvent with an extract concentration of 20% (inhibition zone diameter 20.2 mm). while for S.epidermidis was n-hexane solvent with a concentration of 20% (inhibition zone diameter 23.5 mm). LC50 against E. coli O157:H7 with 96% ethanol and n-hexane as solvents 11.2% and 10.4%, respectively. Meanwhile, the LC50 of S. epidermidis with 96% ethanol and n-hexane solvents were 9.9% and 9.3%, respectively. Qualitatively, white galangal plant extract contains a class of flavonoid compounds, saponins, terpenoids, and tannins.

PEMANFAATAN Trichoderma spp. SEBAGAI BIOKONTROL Sclerotium rolfsii Sacc. PADA TANAMAN KEDELAI (Glycine max L.) Candra Dewi Oktaviawati; Sang Ketut Sudirga; Junita Hardini
SIMBIOSIS Vol 10 No 2 (2022)
Publisher : Jurusan Biologi Universitas Udayana

Sclerotium rolfsii Sacc. on soybeans was one of the diseases in the cultivation that caused a decrease in soybeans (Glycine max L.) production. One of the efforts in increasing the growth and the resistance of the soybean was by using the biological agent named Trichoderma spp. This study aimed to control the intensity of the Sclerotium rolfsii that can cause disease in soybeans. this research was conducted from July 2021 to September 2021 at the Biochemical laboratory and at the Green House owned by the Biology Department FMIPA, Udayana University. The research used an completely randomized design (CRD) with five treatments; a) growing media without the treatment (control); b) growing media + 10 g Trichoderma spp., and without S. rolfsii; c) growing media without Trichoderma spp., + 5 g S. rolfsii; d) growing media + 15 g Trichoderma spp., and 5 g S. rolfsii; e) growing media + 20 g Trichoderma spp., and 5 g S. rolfsii. In this research, the treatment was repeated 5 times and 25 combinations of treatments were obtained. Analysis of Variance (ANOVA) was used in this study to analyze the data, it was found that there was a different result <0,05, then it was continued by using the analysis of Post Hoc Duncan Test. According to the research, it was found that there were two different results. Based on in vitro method, it can be concluded that Trichoderma spp. can inhibit S. rolfsii by about 55,56%. Meanwhile, based on in vivo method, it can be concluded that Trichoderma spp. can inhibit S. rolfsii about 40% which the optimal dose of Trichoderma spp. was about 20 g, the highest plant height was 64.4 cm, the highest average dry weight of shoots was 5.1 g, the highest average dry weight of roots was 0.5 g, and the highest average pod weight was 17.0 g.

Co-Authors Austin Ryan Garrido Candra Dewi Oktaviawati Debora Margareth Deny Christine Sidabutar ENIEK KRISWIYANTI Fransiska Nitti IDA AYU ASTARINI Ida Bagus Gede Darmayasa Jennifer Crismonika MADE PHARMAWATI Meitini Proborini Wahyuni Naomi Anggi Triarta Ni Komang Deny Julyeda NI NYOMAN DARSINI Ni Nyoman Nila Arieswari Ni Putu Ariwathi Nyoman Mega Antari Purnomo Purnomo Rina Sri Kasiamdari SANG KETUT SUDIRGA Santosa Santosa Sebastian S. Cocioba

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