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Journal : JURNAL BIOLOGI INDONESIA

Potensi Hipertoleransi Calopogonium mucunoides, Centrosema pubescens dan Cajanus cajan yang Tumbuh pada Limbah Penambangan Emas Terkontaminasi Sianida dan Merkuri Syarif, Fauzia; Hidayati, Nuril; Juhaeti, Titi
JURNAL BIOLOGI INDONESIA Vol 4, No 4 (2007): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3666.264 KB) | DOI: 10.14203/jbi.v4i4.3251

Abstract

ABSTRACTPotency of Hypertolerance of Calopogonium mucunoides, Centrosema pubescensand Cajanus cajan . Grown on Gold Mine waste Media Contaminated by Cyanideand Mercury. Cleaning up contaminated environment by using green technology ofphytoremediation is urgently needed in the future. These plants will be utilized ashyperaccumulators for cleaning up the contaminated sites. The results of plant screeningshowed that some plant species, which grow in the contaminated areas, indicated hightolerance and potentially effective in accumulating cyanide and mercury in their rootsand above ground portions. Those plants are Calopogonium mucunoides and Centrosemapubescens. This research aims to examine the potency of three candidate plants i.eCalopogonium mucunoides, Centrosemapubescens and Cajanus cajan, that were grownunder three different media i.e tailing waste of PT ANTAM contaminated by cyanide,public mine or PET1 waste that were contaminated by mercury and non contaminated topsoil as a control. The results showed that all of the species were able to grow undercontaminated media and showed a considerable tolerance by indicated high cyanide andmercury accumulation in their shoot and root. Among those three plants, Centrosemapubescens showed the highest level of cyanide and mercury accumulation i.e 3.29 mg I- CN (in the shoot), 34.72 mg I- Hg (in the shoot) and 17.47 mg lk Hg (in the root),followed by Calopogonium mucunoides i.e 14,97 mg I- CN (in the root). Concentrationratio of CN and Hg accumulation in shoot/root of Centrosemapubescens was >I indicatingthat this plant, according to the definition can beconsidered as a hyperaccumulator.Key Words: Calopogonium mucunoides, Centrosemapubescens, Cajanus cajan, mercury,cyanide, waste
Pengujian Ketahanan Kekeringan pada Tanaman Garut (Maranta arundinacea L.) Hasil Mutasi Dengan Radiasi Sinar Gamma Hidayati, Nuril; Sukamto, Lazarus A.; Juhaeti, Titi
JURNAL BIOLOGI INDONESIA Vol 8, No 2 (2012): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (201.008 KB) | DOI: 10.14203/jbi.v8i2.3053

Abstract

Drought Tolerance Assay on Resulted Mutation of Arrowroot Plant (Maranta arundinacea L.) with Gamma Irradiation. Selection of garut (Maranta arundinacea L.) toward drought stress was conducted in induced mutant by using provenance plants from some semi aridregions of East Jawa. In this research three provenance were used 1) Garut from Dusun Pogal, Desa Lebakrejo, Kec. Purwodadi, Kab. Pasuruan (N1); 2) Garut from Dusun Sembung, Desa Parerejo, Kec. Purwodadi, Kab. Pasuruan (N5); 3) Garut from Dusun Genitri, Desa Gunting, Kec. Sukorejo, Kab. Pasuruan (N8). Provenance plants were treated with mutation induction using several levels of gamma radiation i.e. 0, 10, 20 and 40 Gy. The inducted plants were thenplanted in optimum environmental condition for acclimatization. After 5 months the plants were placed in a greenhouse for water stress treatments. Three levels of water regimes 1) optimum water (field capacity Ø = -0,3 to -1,5 Mpa); 2) 7 days watering interval (Ø =-1,0 to -11,0 Mpa); 3) 14 days interval (Ø = -4,0 to -15,0 Mpa). Plant drought tolerance was examined by analyzing morphological and physiological characteristics related to drought tolerant characteristics, including stomatal conductance (stomatal opening), transpiration, rate of CO2 assimilation, biomass production and yield, Harvest Index and drought Tolerance Index. The resultsshowed that radiation treated plants were more capable of maintaining their water potential (Ø). This indicated by significantly higher values of Ø in treated plants i.e. -2.95 Mpa(10 Gy),-2.86 Mpa (20 Gy) and -2.84 Mpa (40 Gy), compared to -3.74 (Untreated plants). Drought stressed plants produced total biomass 79,55 g/plant, much lower compared to unstressed plants (308,20 g/plant). The highest yield was N8 (219,53 g biomass and 139,83 g tuber), followed by N1 (183,32 g biomass and 126,20 g tuber) and N5 (178,8 g biomass and 136,64 g tuber). Drought Tolerance Index of untreated N1 was the highest (1,27), followed by N5 treatedwith 40 Gy (1.22), N1 with 10 Gy (1.17) and N8 with 40 Gy (1.00). Among radiation treatments, untreated plant produced the highest yield followed by the plants treated with 10 Gy, and the lowest was treated with 40 Gy.Keywords: Drought, tolerance, Maranta arundinacea, mutation, gamma, radiation
Uji Potensi Tumbuhan Akumulator Merkuri untuk Fitoremediasi Lingkungan Tercemar Akibat Kegiatan Penambangan Emas Tanpa Izin (PETI) di Kampung Leuwi Bolang, Desa Bantar Karet, Kecamatan Nanggung, Bogor Juhaeti, Titi; Hidayati, N.; Syarif, F.; Hidayat, S.
JURNAL BIOLOGI INDONESIA Vol 6, No 1 (2009): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v6i1.3167

Abstract

ABSTRACTThe research were carried out in Hg contaminated paddy field in Kampung Leuwibolang,Desa Bantar Karet, Kecamatan Nanggung, Kabupaten Bogor. The aim of this research is tostudy the potency of Salvinia molesta, Oryza sativa, Monocharia vaginalis, Limnoharisflava, Paspalum conjugatum, Cyperus monocephala, Centrosema pubescens, Mikania cordataand Commelina nudiflora to accumulate Hg from contaminated soil. The treatments werefertilizer: no fertilizer (as a control), NPK, manure and compost. The result showed that thegrowth of each plant was significantly different. The fertilizer treatments were significantlyaffected plant growth. The S. molesta showed the highest biomass followed by M. vaginalis,O. sativa and C. nudiflora. Meanwhile S. molesta also showed the highest capasity toaccumulate Hg/year followed by C. nudiflora, P. conjugatum dan M. vaginalis. Production ofbiomass and accumulation capasity of contaminant were the characteristic of accumulatorplant. Based on characteristic of hyperaccumulator plant, this research suggested that S.molesta, M. vaginalis, P. conjugatum, O. sativa dan C. nudiflora were selected forfitoremediation of Hg contaminated soil.Keywords: Accumulator plant, Hg, biomass, accumulation capacityKatakunci: Tumbuhan akumulator, Hg, biomas, kapasitas akumulasi
Efektivitas EDTA (Ethylenediaminetetracetic Acid) dalam Meningkatkan Akumulasi Timbal pada Saccharum spontaneum yang Ihmbuh di Limbah Penambangan Emas Hidayati, Nuril; Juhaeti, Titi; Syarif, Fauzia
JURNAL BIOLOGI INDONESIA Vol 4, No 1 (2006): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (758.469 KB) | DOI: 10.14203/jbi.v4i1.3274

Abstract

ABSTRACTEffectivity of EDTA (EthylenediaminetetraceticAcid) in Increasing Lead Accumulationof Saccharum spontaneum Grown Under Gold Mine Waste. Lead (Pb) isknown as one of a major metal contaminant in mine tailing. This metal has low solubility,and in many cases, is not readily available. In most soils capable of supporting plantgrow, the soluble Pb2+ levels are relatively low and will not promote substantial uptake bythe plant. In addition, many plants retain Pb2+ in their roots with only minimal trabsportto the aboveground plant portions. Therefore, it is important to find ways to enhance thebioavailability of Pb2+ or to find specific plants that can better translocate the Pb2+ intoharvestable portions for phytoremediation. The success of Pb phytoremediation is tohave significant Pb availability as well as a large quantity of plant biomass with high rateof growth. This experiment was conducted to obtain both purposes. Saccharumspontaneum which proven tolerant and dominant in contaminated area as well as potentialin producing high biomass was used in this research.The plants were grown in wastemedia added by 100 ppm, 200 ppm and 300 ppm of Pb. Ethylenediaminetetracetine Acid(EDTA) was applied to increase Pb availability and plant uptake as well as translocationto the aboveground portions. The results showed that the plant still capable of growingunder the highest level of Pb. EDTA increase Pb availability and plant uptake. Pb accumulationin the aboveground biomass of EDTA treated plants were relatively higher thanuntreated plants.Key words: Timbal, EDTA, phytoremediation
Produktivitas Klon Katuk (Sauropus androgynus (L.) Merr.) Triploid pada Berbagai Kombinasi Perlakuan Pemupukan dan Waktu Pemangkasan Juhaeti, Titi; Lestari, P.; Utami, N W
JURNAL BIOLOGI INDONESIA Vol 11, No 1 (2015): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3161.361 KB) | DOI: 10.14203/jbi.v11i1.2158

Abstract

Ease of access, affordability and continuity of katuk production is important to support a variety of supplements industry-katuk based being developed at this time. The problem is the existing clones and its cultivation technique has not been able to fulfil the market needs. Therefore clone assembling was done to produce triploid katuk with larger leaves size. In this paper we put forward the research on the fertilization and pruning time that appropriate to support the leaf production of triploid katuk. The study was carried out at the experimental plot in Botany Division of Research Center for Biology-LIPI. The experimental designs was Nested RCBD Design, the main plot were 2 level of pruning time (P1: 3 Weeks After First Pruning (MSPP) and P2: 5 MSPP). The subplots were fertilization (K: Control, B: Beyonic, B ¼: Beyonic + NPK¼, B ½:  Beyonic+ ½ dose NPK and NPK: NPK without Beyonic). The growth parameters observed were plant height, number of leaves, the leaf size, were done every week until 8 weeks after planting (WAP). The first pruning to harvest shoots were done at 8 WAP, and the next pruning were done according pruning time treatment i.e. P1 and P2. The pruning was done 3 times. The variables observed at pruning time were shoot length, number of leaves, leaf size, the pruned fresh weight and the fresh weight of edible parts. The result indicated that the triploid katuk clones showed a good response to fertilization both growth and leaf production. The biofertilizers can be combined with the use of inorganic fertilizers to increase edible part of pruning harvest production. Keywords: pruning time, bioorganic fertilizer, triploid katuk, pruning harvest production 
Respon Fisiologis dan Pertumbuhan Kakao (Theobroma cacao), Kopi (Coffea arabica), Karet (Hevea brasiliensis) dan Cengkih (Syzygium aromaticum ) Fase Bibit Terhadap Naungan dan Pemupukan Hidayati, Nuril; Juhaeti, Titi; Syarif, Fauzia
JURNAL BIOLOGI INDONESIA Vol 11, No 1 (2015): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v11i1.2154

Abstract

Plants show different physiological responses to light intensities. Study on optimum light intensities for different plants is important, especially for commercial plant commodities, such as industrial plantation plants. This research aims to study the influence of shading and fertilization treatments on plant growth and the activity of some physiological process of four industrial trees namely Cacao (Theobroma cacao), Coffee (Coffea arabica ),  Rubber (Hevea brasiliensis) dan  Clove (Syzygium aromaticum). This research was conducted at Cibinong Science Center LIPI. Two treatment factors applied were three levels of shading : 0%, 55%, 75% and three levels of N ferlitizer: 0 g/plant, 5 g/plant and 10 g/plant arranged in Randomized Block Design with five replicates. Result of experiment revealed that all plant species showed the best growth performance and optimum physiological activities under 55% of shade level in combination with 10 g of N fertilization. The highest CO2 was 13.07 µmolm-2s-1  (55%  shad). The highest values of transpiration and stomatal conductance were 7.56 molm-2s-1 dan 0.73 molm-2s-1 (55% shade). The highest carbohydrate content was 22.49% (under 0% shade) and the lowest was 12.74% (under 75% shade). Keywords: Physiological activity, growth, shading, fertilization 
Pertumbuhan dan Produksi Jagung Pulut Lokal Sulawesi Selatan yang Ditanam di Polibag Pada Berbagai Kombinasi Perlakuan Pupuk Organik Rahmansyah, M; Juhaeti, Titi; Hidayati, N
JURNAL BIOLOGI INDONESIA Vol 9, No 2 (2013): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v9i2.168

Abstract

Research has been carried out through the utilization of organic fertilizer to improve the productivity of local maizecultivar originated from South Sulawesi. The study was conducted at the research station of Research Center for Biology,Cibinong Science Center. Corn seed were planted in polybag containing mixture of soil and compost, 6 and 2kg, respectively. The research were carried out by Randomized Complete Block Design arranged in factorial experimentwith four replications. The first factors are three types of local maize namely A: rice corn (pulut beras, BataraKoasa), B: waxy corn (pulut biji, Batara Kamu), C: pulut hibrida (hybrid cultivar). The second factors are 14 combinationtreatment as a mixture of LIPI organic fertilizer (Beyonic-StarTmik, Bio121, EM-121 and MegaRhizo) andanorganic NPK fertilizer. The variables observed were plant growth and production. The results showed that thehybrid cultivar had higher total corn yield productivity compared to the local one, although hybrid cultivar hassmaller plant biomass. Waxy corn (B) accession showed the weight, length and diameter of cob, and also the weightof 100 grains larger than rice corn (A). Fertilization treatments significantly affect the corn-cob productivity which isincludes the weight, length, and diameter units. Fertilization treatments such as EM-121 + (½ dose of NPK), BIO-121 + (½ dose of NPK), and Beyonic + (¼ dose of NPK) showed a good effect on plant, and it was not significantlydifferent with 100% NPK (full doses of NPK). The three corn cultivar showed different responses to fertilizationtreatments.Keywords: local corn, biofertilizer, growth, production
UJI POTENSI TUMBUHAN AKUMULATOR MERKURI UNTUK FITOREMEDIASI LINGKUNGAN TERCEMAR AKIBAT KEGIATAN PENAMBANGAN EMAS TANPA IZIN (PETI) DI KAMPUNG LEUWI BOLANG, DESA BANTAR KARET, KECAMATAN NANGGUNG, BOGOR Juhaeti, Titi; Hidayati, N.; Syarif, F.; Hidayat, S.
JURNAL BIOLOGI INDONESIA Vol 6, No 1 (2009): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v6i1.3167

Abstract

ABSTRACTThe research were carried out in Hg contaminated paddy field in Kampung Leuwibolang,Desa Bantar Karet, Kecamatan Nanggung, Kabupaten Bogor. The aim of this research is tostudy the potency of Salvinia molesta, Oryza sativa, Monocharia vaginalis, Limnoharisflava, Paspalum conjugatum, Cyperus monocephala, Centrosema pubescens, Mikania cordataand Commelina nudiflora to accumulate Hg from contaminated soil. The treatments werefertilizer: no fertilizer (as a control), NPK, manure and compost. The result showed that thegrowth of each plant was significantly different. The fertilizer treatments were significantlyaffected plant growth. The S. molesta showed the highest biomass followed by M. vaginalis,O. sativa and C. nudiflora. Meanwhile S. molesta also showed the highest capasity toaccumulate Hg/year followed by C. nudiflora, P. conjugatum dan M. vaginalis. Production ofbiomass and accumulation capasity of contaminant were the characteristic of accumulatorplant. Based on characteristic of hyperaccumulator plant, this research suggested that S.molesta, M. vaginalis, P. conjugatum, O. sativa dan C. nudiflora were selected forfitoremediation of Hg contaminated soil.Keywords: Accumulator plant, Hg, biomass, accumulation capacityKatakunci: Tumbuhan akumulator, Hg, biomas, kapasitas akumulasi
POTENSI HIPERTOLERANSI CALOPOGONIUM MUCUNOIDES, CENTROSEMA PUBESCENS DAN CAJANUS CAJAN YANG TUMBUH PADA LIMBAH PENAMBANGAN EMAS TERKONTAMINASI SIANIDA DAN MERKURI Syarif, Fauzia; Hidayati, Nuril; Juhaeti, Titi
JURNAL BIOLOGI INDONESIA Vol 4, No 4 (2007): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v4i4.3251

Abstract

ABSTRACTPotency of Hypertolerance of Calopogonium mucunoides, Centrosema pubescensand Cajanus cajan . Grown on Gold Mine waste Media Contaminated by Cyanideand Mercury. Cleaning up contaminated environment by using green technology ofphytoremediation is urgently needed in the future. These plants will be utilized ashyperaccumulators for cleaning up the contaminated sites. The results of plant screeningshowed that some plant species, which grow in the contaminated areas, indicated hightolerance and potentially effective in accumulating cyanide and mercury in their rootsand above ground portions. Those plants are Calopogonium mucunoides and Centrosemapubescens. This research aims to examine the potency of three candidate plants i.eCalopogonium mucunoides, Centrosemapubescens and Cajanus cajan, that were grownunder three different media i.e tailing waste of PT ANTAM contaminated by cyanide,public mine or PET1 waste that were contaminated by mercury and non contaminated topsoil as a control. The results showed that all of the species were able to grow undercontaminated media and showed a considerable tolerance by indicated high cyanide andmercury accumulation in their shoot and root. Among those three plants, Centrosemapubescens showed the highest level of cyanide and mercury accumulation i.e 3.29 mg I-' CN (in the shoot), 34.72 mg I-' Hg (in the shoot) and 17.47 mg lk' Hg (in the root),followed by Calopogonium mucunoides i.e 14,97 mg I-' CN (in the root). Concentrationratio of CN and Hg accumulation in shoot/root of Centrosemapubescens was >I indicatingthat this plant, according to the definition can beconsidered as a hyperaccumulator.Key Words: Calopogonium mucunoides, Centrosemapubescens, Cajanus cajan, mercury,cyanide, waste
PENGUJIAN KETAHANAN KEKERINGAN PADA TANAMAN GARUT (MARANTA ARUNDINACEA L.) HASIL MUTASI DENGAN RADIASI SINAR GAMMA Hidayati, Nuril; Sukamto, Lazarus A.; Juhaeti, Titi
JURNAL BIOLOGI INDONESIA Vol 8, No 2 (2012): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v8i2.3053

Abstract

Drought Tolerance Assay on Resulted Mutation of Arrowroot Plant (Maranta arundinacea L.) with Gamma Irradiation. Selection of garut (Maranta arundinacea L.) toward drought stress was conducted in induced mutant by using provenance plants from some semi aridregions of East Jawa. In this research three provenance were used 1) Garut from Dusun Pogal, Desa Lebakrejo, Kec. Purwodadi, Kab. Pasuruan (N1); 2) Garut from Dusun Sembung, Desa Parerejo, Kec. Purwodadi, Kab. Pasuruan (N5); 3) Garut from Dusun Genitri, Desa Gunting, Kec. Sukorejo, Kab. Pasuruan (N8). Provenance plants were treated with mutation induction using several levels of gamma radiation i.e. 0, 10, 20 and 40 Gy. The inducted plants were thenplanted in optimum environmental condition for acclimatization. After 5 months the plants were placed in a greenhouse for water stress treatments. Three levels of water regimes 1) optimum water (field capacity Ø = -0,3 to -1,5 Mpa); 2) 7 days watering interval (Ø =-1,0 to -11,0 Mpa); 3) 14 days interval (Ø = -4,0 to -15,0 Mpa). Plant drought tolerance was examined by analyzing morphological and physiological characteristics related to drought tolerant characteristics, including stomatal conductance (stomatal opening), transpiration, rate of CO2 assimilation, biomass production and yield, Harvest Index and drought Tolerance Index. The resultsshowed that radiation treated plants were more capable of maintaining their water potential (Ø). This indicated by significantly higher values of Ø in treated plants i.e. -2.95 Mpa(10 Gy),-2.86 Mpa (20 Gy) and -2.84 Mpa (40 Gy), compared to -3.74 (Untreated plants). Drought stressed plants produced total biomass 79,55 g/plant, much lower compared to unstressed plants (308,20 g/plant). The highest yield was N8 (219,53 g biomass and 139,83 g tuber), followed by N1 (183,32 g biomass and 126,20 g tuber) and N5 (178,8 g biomass and 136,64 g tuber). Drought Tolerance Index of untreated N1 was the highest (1,27), followed by N5 treatedwith 40 Gy (1.22), N1 with 10 Gy (1.17) and N8 with 40 Gy (1.00). Among radiation treatments, untreated plant produced the highest yield followed by the plants treated with 10 Gy, and the lowest was treated with 40 Gy.Keywords: Drought, tolerance, Maranta arundinacea, mutation, gamma, radiation