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All Journal Agrotrop : Journal on Agriculture Science Buletin Udayana Mengabdi Journal of Agricultural Science and Biotechnology E-Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Indonesian Journal of Mathematics and Natural Sciences Advances in Tropical Biodiversity and Environmental Sciences
I Ketut Siadi
Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana, Denpasar
Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences Education Environmental Science Mathematics Veterinary

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Journal : Agrotrop : Journal on Agriculture Science

 1 
Aplikasi Rizobakteri Pantoea agglomerans untuk Meningkatkan Pertumbuhan dan Hasil Tanaman Jagung (Zea mays, L.) Varietas Hibrida BISI-2 I GUSTI NGURAH RAKA; KHAMDAN KHALIMI; I DEWA NYOMAN NYANA; I KETUT SIADI
Agrotrop : Journal on Agriculture Science Vol 2 No 1 (2012)
Publisher : Fakultas Pertanian Universitas Udayana

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Abstract

This experiment was conducted in the village of Sibang Gede, District of Abiansemal, BadungRegency on November 2011 until February 2012. The research objective is to enhance the growth andyield of maize hybrid BISI-2 using rhizobacteria Pantoea agglomerans as PGPR in compostformulations. This study used a randomized block design (RBD) single factor with 5 treatments and 5replications. The treatments tested were P. agglomerans BS7a, P. agglomerans BS7b, P. agglomeransBS2a, and P. agglomerans BS5a. All treatments P. agglomerans formulated in the form of compostand given a dose of 50 g/hole. As a control dose of compost 50 g/hole without PGPR. The resultsshowed that the application of rhizobacteria P. agglomerans increased the maximum plant height, netassimilation rate, crop growth rate, chlorophyll content of leaves, fresh weight of roots per plant, ovendried weight of stover per plant, number of seeds per plant, cob length, cob diameter, seed weightmoisture content of 12% per plant and seed yield of 12% water content per hectare.
Efektivitas PGPR Formulasi Kompos Dalam Meningkatkan Ketahanan Tanaman Kedelai terhadap Soybean Stunt Virus I KETUT SIADI; KHAMDAN KHALIMI; I DEWA NYOMAN NYANA; I GUSTI NGURAH RAKA
Agrotrop : Journal on Agriculture Science Vol 7 No 2 (2017)
Publisher : Fakultas Pertanian Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (196.532 KB) | DOI: 10.24843/AJoAS.2017.v07.i02.p12

Abstract

Effectiveness of PGPR Compost Formulation in Improving Soybean Plant Resistance to Soybean Stunt Virus. Soybean stunt virus (SSV) is one of important obstacles of soybean production in Indonesia. This virus causes the stunting on soybean plant and may cause the yield losses up to 71%. Eight isolates of plant growth promoting rhizobacteria (PGPR) were isolated and tested for their efficacy to control SSV on soybean. Those isolates are Paj, Pak2, Pa1, Pa3, Pa4, BT, and KT. Application of PGPR was done by soaking the seeds in PGPR solution prior to planting and application of PGPR in compost formulation. Virus concentration and disease incidence were determined using DAS-ELISA. Results of this study showed that application of PGPR in compost formulation suppressed disease incidence caused SSV. Disease incidence on treated plants ranged between 10% to 25%, while all of plant (100%) on un-treated plants were infected. Peroxidase activity on treated plants increased by 80.25% to 97.33% in comparison with un-treated plants. These results suggested that application of PGPR in compost formulation could increase the resistance of soybean against SSV. Hence, PGPR can be considered as one of measures to control SSV on soybean.
Karakterisasi Biokimia Aeromonas hydrophila dan Potensinya Sebagai Pemacu Pertumbuhan Bibit Tanaman Melon Emas I KETUT SIADI; KHAMDAN KHALIMI; DEWA NYOMAN NYANA
Agrotrop : Journal on Agriculture Science Vol 4 No 1 (2014)
Publisher : Fakultas Pertanian Universitas Udayana

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Abstract

Biochemical Characterization of Aeromonas hydrophila and Its Potential for Growth Stimulatorof Golden Melo Seedlings.The results showed that this research got 20 nitrogen fix bacteria isolate,However, Pg2 isolate could increase the growth of golden melo. The treatment with Pg2 isolatesignificantly increased the golden melo growth. All the plant growth parameters of treated plants weresignificantly higher than those of un-treated control plants (P<0.05). The leaf chlorophyll content, plantgrowth rate, and fresh and dry weights of plant were significantly higher than those of un-treatedcontrol plants. Isolates identified as Enterobacter cloacae PG2 percentage of 98.05% probability.
KARAKTERISASICYMBIDIUM MOSAIC VIRUS (CYMMV) PADA TANAMAN ANGGREK KHAMDAN KHALIMI; KETUT SIADI
Agrotrop : Journal on Agriculture Science Vol 1 No 1 (2011)
Publisher : Fakultas Pertanian Universitas Udayana

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Abstract

Characterization ofCymbidium mosaic virus (CymMV) on Orchid Plant Orchids are affected by more virus disease problems than most crops, reducing their commercial values considerably. Orchid viruses are widespread in cultivated orchids, withCymbidium mosaic potexvirus (CymMV) being the most prevalent. CymMV high incidence in cultivated orchids has been attributed to the stability and ease of transmission of this virus through cultural practices. CymMV induces floral and foliar necrosis. The virus also reduce plant vigor and lower flower quality, which affect their economic value. The objective of the research is to characterize the virus causing mosaic or chlorotic and necrotic on orchids in West Java. A reverse transcription-polymerase chain reaction (RT- PCR) assays using oligonucleotide primers specific to CymMV were also successfully amplified the regions of the coat protein (CP) gene of the virus. Analysis by using sodium dodecyl sulphate- polyacrylamide gel electrophoresis (SDS-PAGE) revealed that the virus have a major structural protein with an estimated molecular weight of 28 kDa. Aligments of partial nucleotide sequences of the CP gene displayed 86 to 92% homology to CymMV isolates from other countries.
POTENSI PSEUDOMONAS AERUGINOSA SEBAGAI AGENS BIOKONTROL TERHADAP SCLEROTIUM ROLFSII SACC. DAN BIOSTIMULAN PADA TANAMAN KEDELAI KHAMDAN KHALIMI; I GUSTI NGURAH RAKA; I KETUT SIADI
Agrotrop : Journal on Agriculture Science Vol 1 No 2 (2011)
Publisher : Fakultas Pertanian Universitas Udayana

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Abstract

Potential of Pseudomonas aeruginosa as Biological Control Agents against Sclerotium rolfsii Sacc. and Biological Stimulants on Soybean Plant. The objectives of this experiment were conducted to evaluate the effectiveness ofP. aeruginosa as an agent of biological control and biological stimulants. P. aeruginosawere tested for antagonistic activity against Sclerotium rolfsii Sacc. The test for antagonistic activity by bacteria was done through side by side culture. Soybean seeds that will be used in the rhizobacteria treatment were soaked in rhizobacteria suspension for 15 minutes. For wihout the treatment, seeds soaked with sterile water instead of rhizobacteria suspension. Results of this experiment,P. aeruginosa showed strong inhibitory activity againstS. rolfsii Sacc. on PDA medium. Percentage of inhibitory activity was 94,4%. Application of P. aeruginosa significantly increased the plant growth. The maximum plant hight, the maximum number of leaves, fresh and dry weights of root, fresh and dry weights of shoot, and chlorophyll content on treated plants significantly higher than those of un-treated control plants according to the Duncan’s multiple range test (P<0.05%). These results suggested that application of rhizobacteria could promote the plant growth and increase the yield.
Produksi Benih Cabai Rawit (Capsicum frutescens L.) Bebas TMV(Tobacco mosaic virus) Melalui Dry Heat Treatment I KETUT SIADI; I GUSTI NGURAH RAKA; I GUSTI NGURAH WISNU PURWADI
Agrotrop : Journal on Agriculture Science Vol 2 No 1 (2012)
Publisher : Fakultas Pertanian Universitas Udayana

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Abstract

The study was done in Denpasar, Bali under greenhouse condition as well as in the field. The objective of the research is to know the effectiveness of dry heat treatment to inactivate TMV which was contaminated chili pepper seeds, and to improves the seeds quality. The seeds which were dry heated under 40ºC for 24 hours and hereinafter 70ºC for 72 hours showed to have no different viability with those of non treated seeds. These indicated that dry heat treatment does not affect to germination of chili pepper seeds. The dry heat treated chili pepper seeds (DHS), non treated ones (NT), and manually TMV-contaminated DHS (DHS+TMV) were planted under field condition. Verification of TMV infection in the 90 days old chili pepper plants by enzyme-linked immunoassay revealed that the rate of TMV infection were 57.89%, 12.5% and 100% for NT, DHS and DHS+TMV. These results indicated that the TMV source was abundance in the field. Infection of TMV in the chili pepper plants given much effect on the plant height, branch number and production. These research results suggest a possibility of using dry heat.
Co-Authors ANAK AGUNG MADE ASTININGSIH GEDE SUASTIKA GEDE SUASTIKA GEDE SUASTIKA Gusti Ngurah Alit Susanta Wirya I Dewa Nyoman Nyana I GEDE PUTU WIRAWAN I Gusti Ngurah Bagus I GUSTI NGURAH BAGUS PRANATA PUTRA I GUSTI NGURAH RAKA I GUSTI NGURAH WISNU PURWADI I KADE DARMAWAN I MADE SUDANA I MADE SUDANA I NYOMAN RAGANATHA I NYOMAN WIJAYA I WAYAN SUKADA I.G.N. Bagus I.M.M. Adnyana I.N. Wijaya K.A. Yuliadhi KADEK DWI UTAMA Khamdan Khalimi NI KADEK AYU WIDAYANTHI NI KADEK DWIK PRADNYAWATI Ni Made Puspawati NI NENGAH DARMIATI NI NYOMAN DWI RESPITA NINGSIH NYOMAN AYU TRISNA ARI UTAMI ROBERTO BOBHOPE ARUAN SHAH KANIGARA ASADDIARI TRISNA AGUNG PHABIOLA WAYAN ADIARTAYASA