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All Journal Jurnal Fitopatologi Indonesia BALI MEDICAL JOURNAL (BMJ) Agrotrop : Journal on Agriculture Science International Journal of Biosciences and Biotechnology Buletin Udayana Mengabdi Journal of Agricultural Science and Biotechnology E-Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Jurnal Ilmu Lingkungan INTERNATIONAL JOURNAL OF APPLIED CHEMISTRY RESEARCH
Ni Made Puspawati
Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Energy Environmental Science Materials Science & Nanotechnology Medicine & Pharmacology

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PENTAMETHYLCARBOXYLATE RUTHENOCENE BASED ANTITUMOUR AGENT Wahjuni, Sri; Puspawati, Ni Made; Williams, Michel
BALI MEDICAL JOURNAL Vol 4 No 2 (2015)
Publisher : BALI MEDICAL JOURNAL

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Abstract

Background: Unlike iron ruthenium is not an essential element for life. However, the behavior of ruthenium compounds in biological systems and, in particular their use as antitumour agents has attracted much attention recently. This study aims to determine antitumour properties against human tumour cell line HeLa of pentamethylcarboxylate ruthenocene. Methods: This is an in vitro study by applying an experimental within post only control group design. Cisplatin, a clinical used medicine was applied for control. The human tumour cell line Hela was used for the test. The cells were cultured at 370C in 5% CO2/air in Roswell Park Memorial Institute Medium 1640 and seeded overnight in 96 well microtitre plates. The penthamethylcarboxylate ruthenocene was dissolved in 1,2 dimethoxy ethane and diluted with culture media. The plates were assays by measuring optical density in the range of 490-655 nm. The D37 values were then calculated. Results: The pentamethylcarboxylate ruthenocene compound tested was found to be more cytotoxic than cisplatin (with D37 = 422 nM compare to D37 = 705 nM for cisplatin). Conclusions: The compound tested, pentamethylcarboxylate ruthenocene was found more potent as an antitumor compare to cisplatin a clinical used antitumor for curing testicular carcinoma.
AKTIVITAS ANTIBAKTERI FRAKSI EKSTRAK ETANOL DAUN TREMBESI (SAMANEA SAMAN (JACQ.) MERR) TERHADAP STAPHYLOCOCCUS AUREUS Sinarsih, Ni Ketut; Rita, Wiwik Susanah; Puspawati, Ni Made
International Journal of Applied Chemistry Research Vol 3, No 1 (2021)
Publisher : Universitas Pendidikan Ganesha

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23887/ijacr.v3i1.32860

Abstract

Penelitian ini dilakukan untuk menentukan aktivitas antibakteri fraksi-fraksi dari  hasil partisi ekstrak etanol daun trembesi dalam menghambat pertumbuhan bakteri S. aureus, yang meliputi ekstraksi daun trembesi dengan pelarut etanol, partisi menggunakan pelarut n-heksan, etil asetat, dan n-butanol, penentuan daya hambat fraksi hasil partisi, serta penentuan Konsentrasi Hambat Minimum (KHM) dari fraksi yang menunjukkan aktivitas penghambatan terbaik untuk menghambat pertumbuhan S. Aureus. Hasil penelitian menunjukkan bahwa dari 2000 gram sampel diperoleh ekstrak kental sebanyak 160 gram. Proses partisi menghasilkan empat fraksi yaitu fraksi n-heksan yang berwarna hijau tua, fraksi etil-asetat yang berwarna hijau kecoklatan, fraksi n-butanol yang berwarna coklat tua, dan fraksi air yang berwarna coklat pekat. Dari keempat fraksi, hanya fraksi n-butanol yang memberikan efek penghambatan terhadap S. aureus secara in vitro pada media Mueller Hinton, yaitu sebesar 19,3 mm pada konsentrasi 15%. Pada konsentrasi tersebut, aktivitas antibakteri fraksi n-butanol dapat dikategorikan kuat terhadap S. aureus. Pengujian KHM dilakukan pada tiga konsentrasi yaitu 0,5; 1; dan 1,5% dengan diameter hambat aktivitas antibakteri secara berturut -turut sebesar 0 mm; 7,3 mm; dan 9,2 mm.
Penyakit Layu Bakteri Stewart pada Jagung di Bali I Gede Rai Maya Temaja; G.N. Alit Susanta Wirya; Ni Made Puspawati; Khairun Nisak Syahdu
Jurnal Fitopatologi Indonesia Vol. 13 No. 5 (2017)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (414.406 KB) | DOI: 10.14692/jfi.13.5.184

Abstract

Stewart’s wilt is a serious disease of sweet corn (Zea mays). The typical symptoms of the disease are pale-green to yellow linear streaks parallel to the veins. The symptoms were observed on sweet corn in Denpasar, Tabanan, Gianyar, and Karangasem areas during a survey in 2015. Pathogen detection based on a polymerase chain reaction was carried out using total DNA obtained from symptomatic leaf samples and the pairs of primers, CPSL1/CPSR2c. The expected sized (~1100 bp) amplicon was detected in samples from Denpasar. Sequence analysis confirmed that Stewart’s wilt disease symptoms are caused by Pantoea stewartii subsp. stewartii. Nucleotide sequence and phylogenetic analysis showed that P. stewartii subsp. stewartii from Bali has high homology (98.97-99.08 %) and placed in the same clade with isolates from Canada, USA and Japan. This is the first report of P. stewarti subsp. stewartii on corn in Bali.
UTILIZATION OF RHIZOSPHERE FUNGI TO CONTROL Fusarium oxysporum f.sp. capsici IN VITRO I Made Sudarma; Ni Made Puspawati; Ni Wayan Suniti; I Nyoman Wijaya; I Gusti Ngurah Bagus
International Journal of Biosciences and Biotechnology Vol 2 No 2 (2015)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

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Abstract

Fusarium caused wilt disease in chilli pepper and destroyed some farmer crops. Results ofpreliminary research has been discovered that the disease caused by Fusarium oxysporum f.sp. capsici.The alternative environmental friendly method is to find antagonist microbes which is located in therhizosphere of healthy pepper plants. This study aims to find out potentially antagonistic fungi tocontrol Fusarium wilt disease on pepper plants. The fungi were isolated by soil dilution method orviable plate count method on Potato Dextrose Agar medium with antibiotic Livoplaxacin (25%, w/v).Rhizosphere fungi from healthy pepper plants had been identified. A total of 63 spesies belong to 4genera included Penicillium (45 species), Aspergillus (6 species), Trichoderma (9 species) andCandida (3 species). The highest percentage of distribution of rhizosphere fungi are P. digitatum(47.63%), P. expansum (19,05%), T. harzianum (9,53%), A. nidulans, A. niger, Penicillium sp.,Candida albicans, and T. vitrens i.e 4,76% respectively. All of rhizosphere fungi colonies were foundto inhibit Fusarium oxysporum f.sp. capsici in vitro. The best inhibition was found in Aspergillusniger at 88.89 ± 2.2% followed by A. nidulans of 85,56 ± 1,6 %, T. harzianum at 84,45 ± 1,58% , andT. virens by 83,33 ± 1,2%, five days after inoculation. All of them have a very high inhibition criteria.
PELATIHAN PENGENDALIAN PENYAKIT TUNGRO DAN BLAS PADA TANAMAN PADI DI SUBAK BASANGKASA IGRM TEMAJA; M SUDANA; IP SUDIARTA; GNA SUSANTA WIRYA; NM PUSPAWATI
Buletin Udayana Mengabdi Vol 14 No 1 (2015): April 2015
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

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Abstract

Training on tungro and blast diseases was held at Subak Basangkasa, Kerobokan village, Badung regency onApril 27, 2014. The activities was conducted to educate farmers how to control tungro and blast diseases. Themethods used in this activity were lectures, demonstration and practice in the paddy field. The training wasattended by 30 participants from local farmer groups of Subak Basangkasa. Based on post test, more than 90% ofthe farmers managed to answer the questions about tungro and blast diseases, including pathogens, symptoms ofdiseases, factors affecting the growing of diseases as well as the control of diseases. The data indicated on the finalof training all participants completely understand about the topics. All participants participated enthusiasticallyand hope they have the next intensive training again.Key words :disease control, tungro disease, blast disease, trainin
STRATEGI PENGENDALIAN PAPAYA RINGSPOT VIRUS (PRSV) PENYEBAB PENYAKIT BERCAK BERCINCIN PADA PEPAYA I Gede Rai Maya Temaja; I Putu Sudiarta; Ni Nengah Darmiati; Ni Made Puspawati
Journal of Agricultural Science and Biotechnology Vol 5 No 1 (2016)
Publisher : Journal of Agricultural Science and Biotechnology

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Abstract

ELISA test results of symptomatic leaves from Tabanan, Bali showed that papaya ringspot virus (PRSV) as causing agents of ring spot disease on papaya in Bali. The objective of the study were to determine the control strategies of ringspot disease on papaya in the field. Control strategies of ringspot disease tested in this study were the use of metallic gray plastic mulch and plant barrier. Research result showed that plant barrier can provide a buffer zone to protect papaya that are planted within the confines of the buffer.
UTILIZATION OF SDT-RT-PCR FOR PLANT VIRUS DETECTION I Gede Rai Maya Temaja; Ni Made Puspawati; Ni Nyoman Ari Mayadewi
Journal of Agricultural Science and Biotechnology Volume 1, No 1, Tahun 2012
Publisher : Journal of Agricultural Science and Biotechnology

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Abstract

Metode simple-direct-tube (SDT) dikembangkan untuk preparasi     RNA virus atau viroid, yang selanjutnya dipakai untuk sintesis cDNA. SDT tidak memerlukan penyaringan atau sentrifugasi seperi pada ekstraksi RNA total menggunakan KIT. Metode ini juga tidak menggunakan antiserum dalam preparasi RNA virus. Di samping itu metode SDT dapat dikerjakan di laboratorium dengan temperatur ruang dan selesai dikerjakan lebih kurang dalam 25 menit. Penerapan metode ini dilanjutkan dengan reverse transcription-polymerase chain reaction (RT-PCR), dapat mendeteksi keberadaan tomato chlorosis virus (ToCV), tomato infectious chlorosis virus (TICV), chrysanthemum virus B (CVB), Potyvirus and chrysanthemum stunt viroid (CSVd). Hal ini menunjukkan bahwa metode SDT dapat dipakai untuk mendeteksi virus atau viroid.
Uji Efektifitas Ekstrak Daun dari Beberapa Jenis Tanaman untuk Mengendalikan Nematoda Puru Akar Meloidogyne Spp. pada Tanaman Cabai (Capsicum Annum L) IDA BAGUS MADE DWI JAYA; MADE SRITAMIN; NI MADE PUSPAWATI
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.3, No.2, April 2014
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Abstract

ABSTRACT Root knot nematodes (Meloidogyne spp.) is an important class of nematode that causes significant reduction in the production of Capsicum annum L. Various control measures were taken to suppress the population of Meloidogyne spp. such as planting resistant varieties, crop rotation and technical culture, still unwell provide fast response compared to chemical control. Biopesticides is a way of controlling the use of more environmentally friendly than synthetic pesticides. Based on this, it will be carried out studies on the control of root knot nematodes Meloidogyne spp. using plant leaves tembelekan (Lantana camara), kirinyuh (Chromolaena odorata), papaya (Carica papaya), lemongrass (Cymbopogon nardus L.), betel (Piper betle L.) and tobacco (Nicotiana tabacum) which extracts used were 250cc / pots of each extract solution leaves. The purpose of this study was to determine the plant leaf extract is capable of suppressing the population of Meloidogyne spp. and determine the most effective leaf extracts suppress the population of Meloidogyne spp. The results of this empirically found that population of nematoda per 300 grams of soil extract of Piper betle L. can suppressed the nematode population of Meloidogyne spp. The best is 23.4 nematodes or 95.32%, followed by treatment of the extract of C. odorata 27.6 nematodes (94.48%), L. camara 28.8 nematodes (94.24%), Carica papaya 43, 4 nematodes (91.32%), Cymbopogon nardus L. 53.8 nematodes (89.4%), and Nicotiana tabacum 60.8 nematodes (87.84%). Population of nematoda per 1 g of root, extract of Piper betle Linn. is also the most well extract in suppressing root knot nematode populations, there are only recorded 21.2 percentage suppression tail with 95.76%, followed by treatment of C. odorata extract 23.6 tail (95.28%), L. camara 24 , 2 goats (95.16%), Carica papaya 28.4 tail (94.32%), Cymbopogon nardus (L). 30.2 tail (93.96%), and Nicotiana tabacum 35.2 tail (92.96%). Keyword: Capsicum annum L, Carica papaya, Chromolaena odorata, Cymbopogon nardus L., Lantana camara, Meloidogyne spp., Nicotiana tabacum, Piper betle Linn.
Pengaruh Inokulasi Trichoderma sp. Indigenus terhadap Penyakit Akar Gada dan Pertumbuhan Tanaman Kubis (Brassica oleracea L.) NI PUTU MERRY SENI ANTARI; NI MADE PUSPAWATI; I KETUT SUADA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.6, No.4, Oktober 2017
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Abstract

The Influence of Indigenous Trichoderma sp. Inoculation against Clubroot and the Growth of Cabbages (Brassica oleracea L.) The Cabbage (Brassica oleracea L.) is a plant that contains many vitamins and minerals needed by human. The problem which often experienced by cabbage farmers is clubroot deseases that caused by pathogenic Plasmodiophora brassicae Worr. Eco-friendly controls are needed so as not to adversely affect ecosystems by using natural enemies of the pathogen such as Trichoderma sp. The purpose of this research was to obtain the type Trichoderma sp. which effectively suppress clubroot and increase the growth of cabbage plants. The results showed that the most effective isolate was isolate from zucchini plant (Cucurbita pepo L.) rhizosphere (T1), followed by tomato plant (Lycopersicon esculentum L.) rhizosphere (T2), romana plant (Lactuca sativa L. var. longifolia) rhizosphere (T4), and from the spinach plant (Spinacia oleracea L.) rhizosphere (T9). The isolates were able to decrease clubroot and promote cabbage plant growth as well.
Pengaruh Penyakit Virus Mosaik dan Kuning Terhadap Hasil Panen Tanaman Kacang Panjang (Vigna sinensis L.) Di Desa Perean, Baturiti, Tabanan NI NYOMAN ALIT PURWANINGSIH; NI MADE PUSPAWATI; I DEWA NYOMAN NYANA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.5, No.3, Juli 2016
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Abstract

Influence Mosaic Diseases and Yellow to Long Bean (Vigna sinensis L.)Production at Perean Village, Baturiti, TabananThis study aims to determine how far the influence of viral infection with symptoms of mosaic and yellow against a decrease in crop yield long beans. This research consists of four activities are: 1. Determining the spread of disease areas; 2. Observation and data collection; 3. Calculation yields; and 4. Data analysis. Design used was a randomized block design (RBD) with three treatments and nine replications.Research result show that long bean plants in several locations in Perean village infected by BCMV which has mosaic symptoms and MYMV which has yellow symptoms. Percentage of mosaic virus symptoms is higher than yellow virus. The average of percentage of mosaic virus symptoms was 42,85%, while yellow virus was 32,70%. Plant beans with yellow virus symptoms causes the highest yield loss which amounted to 53,87% and plants with symptoms of mosaic virus by 43,02% when compared with healthy plants. Based on these studies it is necessary dissemination of information, about the importance of sanitation and the use of healthy seeds to controlling the long bean’s viral infection to related parties.
Co-Authors DWI WIDANINGSIH GEDE SUASTIKA Gusti Ngurah Alit Susanta Wirya I Dewa Nyoman Nyana I G. R. M. TEMAJA I Gusti Ngurah Bagus I GUSTI NGURAH BAGUS PRANATA PUTRA I Ketut Siadi I Ketut Suada I MADE SUDARMA I MADE SUDARMA I MADE SUDARMA I MADE SUDARMA I MADE SUDARMA I MADE SUDARMA I NYOMAN WIJAYA IDA BAGUS MADE DWI JAYA KETUT AYU YULIADHI Khairun Nisak Syahdu KHAMDAN KALIMI M SUDANA MADE SRITAMIN Michel Williams, Michel Muhammad Ikhsan Nulzaen NI NENGAH DARMIATI NI NYOMAN ALIT PURWANINGSIH NI NYOMAN ARI MAYADEWI NI PUTU MERRY SENI ANTARI NI WAYAN SUNITI NYOMAN RAI KUNTALINI OCTA FRANSISCA SITORUS ONGKY ARI WIBOWO PANDE MADE INDRAYOGA Putu Sudiarta Sinarsih, Ni Ketut Sri Wahjuni WIWIK SUSANAH RITA