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Dwi Priyowidodo
Departemen Parasitologi Fakultas Kedokteran Hewan Universitas Gadjah Mada
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology Social Sciences Veterinary

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Studi Respon Imun Humoral Mencit, Tikus, dan Ayam terhadap Infeksi Toxoplasma Gondii Gifti Rosalina Ratnaningrum; Dana Meida; Andhini Mutiara Putri; Hilda Syara Shita Devi; Dwi Priyowidodo
Jurnal Sain Veteriner Vol 34, No 2 (2016): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (6673.961 KB) | DOI: 10.22146/jsv.27556

Abstract

Toxoplasmosis is a disease caused by the protozoan Toxoplasma gondii. T. gondii is an obligate intracellular parasite that is transmitted by congenital and acquisition. T. gondii can infect all warm-blooded animals and are zoonotic. It is estimated that more than a third of the world’s population is infected by T. gondii. Chicken is one of the animals that can be infected with T. gondii and plays an important role in the spread of this disease to humans because it is one of the animals used as a source of animal feed. Mice and rats are also play an important role in the spread of toxoplasmosis in the definitive host, especially in cats. T. gondii infection is usually asymptomatic, but in certain circumstances, such as when the host’s immune system is weakened, this parasite can causeserious illness. This study were aimed to determine the humoral immune response against T. gondii infection in mice, rats and chickens by serological Latex Agglutination Test (LAT) using Pastorex-Toxo® kit. Two weeks oldbroiler chickens were 8 heads, Wistar strain rats aged 8 weeks as many as 18 heads and strain Balb C mice aged 8 weeks as many as 13 cows used in this study. Animals were grouped into two groups, namely the control andinfection groups. Mice, rats, and chickens were infected each with 103, 107 and 105 takizoit by intra-peritoneal injection. Blood samples were taken from the animals and infection control from day to-1, 2, 3, 4, 5, 6 for mice,the to-1, 2, 3, 4, 5, 6, 7, 8 for rats, and day - 2, 4, 14 for chicken by intracardia 3 ml, then put in a non-EDTA tubes and centrifuged at a speed of 10,000 rpm for 5 minutes to collect serum. The results showed a humoral immuneresponse against T. gondii in rats began to appear on day 5 post-infection, chickens began to emerge the 2nd day post infection, whereas mice do not indicate a humoral immune response against T. gondii post infection.
Akumulasi Fibrin dalam Anterior Chamber Pada Kucing Penderita Tripanosomiasis dan Feline Immunodeficiency Virus Kurnia Kurnia; Dyah Kunthi Wirapratiwi; Setyo Budhi; Guntari Titik Mulyani; Dwi Priyowidodo
Jurnal Sain Veteriner Vol 39, No 1 (2021): April
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.54614

Abstract

Tripanomiasis merupakan penyakit yang disebabkan oleh Tripanosoma sp, protozoa hemoflagellata dari kelas Zoomatigophora dan famili Tripanosomatidae. Tripanosomiasis banyak dijumpai di daerah tropis dan menyerang berbagai hewan domestik seperti kuda, sapi, kerbau, onta, anjing, kucing dan tikus. Feline immunodeficiency virus (FIV) dikenal sebagai  feline AIDS adalah spesies virus dalam genus Lentivirus, menyebabkan penurunan sistem imun pada kucing dimana tubuh tidak dapat mengatasi serangan dari berbagai sumber penyakit lain sehingga muncul infeksi tambahan. Umumnya kucing tidak menimbulkan gejala klinis infeksi FIV meskipun telah berlangsung beberapa tahun. Seekor kucing domestik jantan, 3 tahun didiagnosis Tripanosomiasis dan positif FIV. Kedua mata terlihat berwarna keputihan, berawal dari mata kiri, berukuran kecil yang berkembang secara progresif selama 2 minggu, diikuti penurunan nafsu makan serta kondisi badan yang semakin kurus. Kucing berasal dari kucing jalanan, dan gemar memakan tikus maupun burung, serta belum dilakukan vaksinasi. Hasil pemeriksaan klinis menunjukkan mukosa anemik, dehidrasi, oedema di daerah submandibular hingga bahu, BCS 4/9, dan uveitis anterior. Hasil pemeriksaan hematologi dan kimia darah menunjukkan anemia normositik-hipokromik, trombositophenia, normal leukosit total dengan peningkatan relatif monosit, nilai SGPT dan SGOT yang sangat tinggi, peningkatan creatinin dan penurunan total kolesterol. Hasil pemeriksaan rapid test menunjukkan positif antibodi FIV (Feline Immunodeficiency Virus), negatif Feline Leukemia Virus, serta negatif  toksoplasma. Pemeriksaan apus darah menunjukkan mild anemia tanpa polikromasia, dan ditemukan flagellata Trypanosoma sp. Akumulasi fibrin di dalam anterior chamber yang bersifat progresif-bilateral disertai dengan aqueous flare dan normal retina merupakan gambaran anterior uveitis sebagai gejala klinis yang menciri dari Tripanosomiasis pada kucing dan infeksi FIV.
Diagnosis Toksoplasmosis Kongenital Berdasarkan Gen Surface Antigen-1 Toxoplama gondii Isolat Lokal Menggunakan Polymerase Chain Reaction (DIAGNOSIS OF CONGENITAL TOXOPLASMOSIS BASED ON SURFACE ANTIGEN -1 GENE OF LOCAL ISOLATE TOXOPLASMA GONDII USING POLY Dwi Priyowidodo; Sri Hartati; Asmarani Kusumawati; Joko Prastowo
Jurnal Veteriner Vol 16 No 3 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (122.315 KB)

Abstract

Congenital toxoplasmosis has an important role in the transmission of toxoplasmosis in animals andhumans. Thus, a rapid and an accurate diagnostic method is needed. The aim of this study was to conductthe diagnosis technique of congenital toxoplasmosis in mice based on surface antigen-1 (SAG-1) gene oflocal isolates (IS-1) T. gondii using Polymerase Chain Reaction (PCR). A total of 15 pregnant mice Balb/C strain with the aged of eight weeks were used as experimental animal. Mice were intraperitoneallyinfected with 103tachizoit of T. gondii RH strain at day 9th of gestation. Amniotic fluids, blood, fetus, andplacenta then were collected at day 1, 2 , 3, 4 and 5 post infection. DNA was extracted from the abovesamples using PureLinkTM Genomic DNA Kit (Invitrogen, Life Technologies, US), and then amplified byusing specific primer based on SAG-1 gene of the local isolate T. gondii. This study shows that positivePCR result were seen in all samples of amniotic fluids at day 2 up to day 5 post infection. Fetus andplacenta samples also show positive PCR result at 3 up to day 5 post infection. Negative PCR result showsin blood samples, however. To conclude, PCR technique using SAG-1 gene of local isolates T. gondii as atarget gene, could be used to detect congenital toxoplasmosis from infected mouse samples such as, amnionfluids, fetus, and placenta. Further research was needed to apply the PCR method with SAG-1 gene of localisolate T. gondiion the human samples of congenital toxoplasmosis.
Identifikasi Cacing Trematoda dan Gambaran Patologi Ginjal Burung Merpati yang Terinfeksi (IDENTIFICATION AND PATHOLOGICAL FEATURES OF TREMATODE IN PIGEON’S KIDNEY) Ana Sahara; Joko Prastowo; Dwi PriyoWidodo; Eryl Sri Rohayati; Sitarina Widyarini
Jurnal Veteriner Vol 14 No 4 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

This study is conducted to identify species of trematode and its pathological features in pigeons’kidney. Twenty five of Yogyakarta pigeons were examined for trematode infection in kidneys. One ofkidneys was mashed in mortar with a little water, the other was examined for histopathological featuresstained with hematoxyline-eosin.Trematodes found were stained with Schmison ´s Carmine. Seven pigeons(28%) were infected by trematode with non significant clinical features. Identification trematodescharacterized by oral sucker, pharynx, testes are slighty diagonal in position, irregular in shape and intracaecal. Ovary pretesticular and vitellaria widespread from anterior ovarium to the posterior body.Histopathological examination showed segment of trematode in the medullary collecting ducts lumen,dilatation, flattening and emptyness of ducts epithelial cells, emptyness, giant cells and dominationmononuclear cell in interstitial tissue, characterizing a granulomatous nephritis. Trematodes foundidentified as Paratanaisia bragai. The trematodes were found in very dilated medullary collecting ductsand caused inflammation in adjacent tissues. Further studies are needed to find out vector of trematodein pigeons .
PREVALENSI DAN INSIDENSI PARASIT PADA IKAN MAS (CYPRINUS CARPIO) . Dwilantiani; Yudhi Ratna Nugraheni; Joko Prastowo; Dwi Priyowidodo; Ana Sahara; Wisnu Nurcahyo
Partner Vol 24, No 2 (2019): Edisi November 2019
Publisher : Politeknik Pertanian Negeri Kupang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35726/jp.v24i2.285

Abstract

Infestasi parasit pada ikan dalam jumlah besar dapat menyebabkan kerugian ekonomi yang cukup signifikan. Parasit dari jenis Trematoda monogenean dan protozoa mendominasi infestasi tersebut.  Penelitian ini dilakukan untuk mendapatkan status kejadian infestasi parasit pada ikan mas biasa (Cyprinus carpio). Sebanyak 200 ikan mas (Cyprinus carpio) dikumpulkan dari kolam-kolam di Sleman dan Magelang, Jawa Tengah Indonesia. Metode pemeriksaan menggunakan metode wet mount. Parasit yang ditemukan sebagai berikut: Ichthyopthirius multifilis 41% Dactylogyrus  spp. 44,5% dan Gyrodactylus spp. 25%. Kejadian insidensi parasit sebagai berikut: Ichthyopthirius multifilis 1496/82, Dactylogyrus  spp. 119/37 dan Gyrodactylus spp. 2/2. Kondisi kolam yang buruk dan pergantian musim di Indonesia merupakan faktor yang sangat berpengaruh terhadap kejadian infestasi parasit pada ikan mas (Cyprinus carpio).
Ehrlichiosis pada kucing yang mengalami anemia dan indikasi gagal ginjal Kurnia Kurnia; Dito Anggoro; Setyo Budhi; Dwi Priyowidodo
ARSHI Veterinary Letters Vol. 4 No. 2 (2020): ARSHI Veterinary Letters - Mei 2020
Publisher : School of Veterinary Medicine and Biomedical Sciences, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (524.271 KB) | DOI: 10.29244/avl.4.2.23-24

Abstract

Kucing persia diperiksa sebanyak 2 ekor diperiksa tanggal 23 April 2019 dan 29 April 2019 dengan gejala lesu, mukosa pucat, tidak mau makan sejak 3-5 hari, dehidrasi dan mengalami penurunan berat badan dalam satu bulan terakhir. Kucing tersebut berasal dari pemilik berbeda yang memungkin pernah kontak dengan anjing. Pemeriksaan klinis menunjukkan kedua kucing mengalami anemia, lethargi dan abnormalitas ukuran ginjal yang membengkak pada kucing I dan atropi pada kucing II. Hasil pemeriksaan laboratorium kedua kucing mengalami anemia, SGPT/ALT turun, albumin normal, blood urea nitrogen (BUN) dan creatinin keduanya meningkat. Kucing I mengalami trombositopenia, leukositosis dengan neutrofilia dan protein plasma normal. Kucing II menunjukkan trombosit normal, neutrofilia dan total protein yang meningkat. Pemeriksaan preparat apus darah ditemukan inklusi intrasitoplasmik dalam neutrofil dari kedua kucing yang mengarah pada morula Ehrlichia sp. Diagnosa kedua kucing mengarah pada dugaan Ehrlichiosis. Penanganan anemia dan dehidrasi diberikan infus NaCl 0,9% intravena, injeksi Meylon dan Hematodin. Kondisi kedua kucing terus menurun, kucing I mati setelah 2 hari terapi dan kucing II mati setelah 5 hari terapi.
Parasit Gastrointestinal pada Sapi di Daerah Aliran Sungai Progo Yogyakarta Yudhi Ratna Nugraheni; Dwi Priyowidodo; Joko Prastowo; Eryl Sri Rohayati; Ana Sahara; Aan Awaludin
Jurnal Ilmu Peternakan Terapan Vol 1 No 2 (2018): Jurnal Ilmu Peternakan Terapan
Publisher : Politeknik Negeri Jember

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Abstract

The aim of study was investigate gastrointestinal disease on cow fed forage from Progo watershed. Sixty three feses samples were taken from cows on Progo watershed. The method of feses analysis used was sentrifuge and Parfitt Bank. The results showed that 63 samples of cows feces were examined, among 25 were infected by Fasciola sp., 7 Paramphistomum sp., 14 Strongyle, and 9 Coccidiosis. The conclusion was the prevalence of gastrointestinal disease in cows was dominant incidence of Fasciolosis by 40%, Strongyle 22%, 14% Coccidiosis, and Paramphistomum sp.11%.
Diagnosis dan Pengobatan Babesiosis pada Anjing Shih-Tzu di Klinik Lilipoet, Yogyakarta, Indonesia Nabilah Putroe Agung; Dwi Priyowidodo; Ida Tjahajati; Lily Gunawan
Jurnal Sain Veteriner Vol 40, No 3 (2022): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.74202

Abstract

Dogs are one of the pet animals that have a loyal nature and are widely cared for in the community. It is not uncommon for dogs to often play in parks or outdoor environments that are surrounded by grass to play with their owners. The grass is a breeding ground for ticks, which can be vectors of disease carriers for dogs. Babesiosis is a disease caused by Babesia sp. and is transmitted by tick vectors. In this paper, we will discuss the clinical condition, diagnosis, and treatment of one dog with babesiosis at the Lilipoet clinic, Yogyakarta. A Shih-Tzu dog has clinical symptoms of fever with a temperature of 39.9oC, decreased appetite, and has a history of yellow vomiting, and found one tick. Blood was collected for microscopic examination of blood smears, blood hematologic, and molecular examination. Hematologic examination of the blood showed the value of thrombocytopenia with a value of 32 x 103/µL. Molecular examination with Polymerase Chain Reaction (PCR) showed positive results of Babesia sp. with visible DNA bands at 490bp. Treatment was carried out by giving one dose of antiprotozoal diminazen aceturate 3.5 mg/kg BW and doxycycline 10mg/kg BW once a day for 14 days.
Strongyloidiasis in Bornean Orangutan: A Case Report Putu Suandhika; Agatha Ria Susanti; Agnes Pratamiutami Sriningsih; Carlo Yunior Ray Hina; Yanuartono Yanuartono; Soedarmanto Indarjulianto; Dwi Priyowidodo
Jurnal Medik Veteriner Vol. 6 No. 2 (2023): October
Publisher : Faculty of Veterinary Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol6.iss2.2023.297-302

Abstract

This study reported the diagnosis and treatment of Strongyloidiasis in two Bornean orangutans (Pongo pygmaeus); adult male aged 23 years, weighing 100 kg and an infant female orangutan, 4 years old, weighing 13,6 kg. Samples were physically and laboratory examined for faeces using the Mini-FLOTAC method. Therapy was performed on the adult orangutan using albendazole at a dose of 400 mg individual twice a day for 4 days, orally. The infant orangutan was administered albendazole at a dose of 10 mg/kg BW once a day for 3 days orally. From several physical examinations, both orangutans looked active, body temperature and oral mucosa color were normal, green feces with solid consistency in the adult orangutan and brown with solid consistency in the infant orangutan. Microscopic faeces examination of the adult orangutan showed Strongyloides sp. as many as 1005 eggs per gram (EPG) of feces, and the infant orangutan as many as 2490 larvae per gram of feces. Both orangutans were diagnosed with severe Strongyloidiasis based on those examinations. Three days after starting treatment, the adult orangutan's EPG decreased to 890, and a week later, no Strongyloides sp. eggs or larvae were discovered. A week following treatment, no Strongyloides sp. was discovered in an infant orangutan. In conclusion, albendazole was an effective treatment for severe Strongyloidiasis in adult and infant orangutans. Strongyloidiasis can be a threat to both infant and adult orangutans without distinct clinical symptoms.
Co-Authors . Dwilantiani Agatha Ria Susanti Agnes Pratamiutami Sriningsih Ana Sahara Ana Sahara Andhini Mutiara Putri Asmarani Kusumawati Awaludin, Aan Carlo Yunior Ray Hina Dana Meida Dito Anggoro Dyah Kunthi Wirapratiwi Eryl Sri Rohayati Gifti Rosalina Ratnaningrum Guntari Titik Mulyani Hilda Syara Shita Devi Ida Tjahajati Joko Prastowo Kurnia Kurnia Kurnia Kurnia Lily Gunawan Nabilah Putroe Agung Nugraheni, Yudhi Ratna Putu Suandhika Raden Wisnu Nurcahyo Sahara, Ana Setyo Budhi Setyo Budhi Sitarina Widyarini Soedarmanto Indarjulianto Sri Hartati Yanuartono Yanuartono