Article Per Year (5 Year)
p-Index From 2019 - 2024
0.23
P-Index
This Author published in this journals
All Journal BIOTROPIA - The Southeast Asian Journal of Tropical Biology BERITA BIOLOGI JURNAL BIOLOGI INDONESIA Jurnal Bioteknologi & Biosains Indonesia (JBBI) ANNALES BOGORIENSES OLDI (Oseanologi dan Limnologi di Indonesia) Marine Research in Indonesia Pharmaceutical Sciences and Research (PSR) Makara Journal of Science
PUSPITA LISDIYANTI
Pusat Penelitian Bioteknologi, Lembaga Ilmu Pengetahuan Indonesia (LIPI)
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Environmental Science Immunology & microbiology Medicine & Pharmacology Veterinary

Published : 101 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 101 Documents
Search

 7   8   9   10   11 
Enzymatic Hydrolysis of Mannan from Konjac (Amorphophallus sp.) Using Mannanase from Streptomyces lipmanii to Produce Manno-oligosaccharides Sasongko, Ashadi; Yopi,; Rahmani, Nanik; Lisdiyanti, Puspita; Saepudin, Endang
Makara Journal of Science
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Mannan is an abundant polysaccharide that can be found in konjac (Amorphophallus sp.). Mannan can be enzymatically hydrolyzed using mannanase to produce manno-oligosaccharides which can be used as a prebiotic. The aims of this research are to determine the production time of mannanase from Streptomyces lipmanii, perform enzyme characterization, optimize the hydrolysis time, and characterize the hydrolysis product. A qualitative assay using the indicator Congo red showed that S. lipmanii generated a clear zone, indicating that S. lipmanii produced mannanase in konjac medium and possessed mannanolytic activity. Enzyme activity was determined through reducing sugar measurement using the dinitrosalycylic acid method, and optimum enzyme production was achieved at the second day of culture. Characterization of the enzyme showed that hydrolysis was optimum at pH 7 and at a temperature of 50 oC. The reducing sugar content was increased by an increasing the hydrolysis time, and reached an optimum time at 2 h. The degree of polymerization value of three was achieved after 2 h hydrolysis of mannan from konjac, indicating the formation of oligosaccharides. Analysis by thin layer chromatography using butanol, acetic acid, and water in a ratio of 2:1:1 as eluent showed the presence of compounds with a retention time between those of mannose and mannotetrose. Confirmation was also performed by HPLC, based on the retention time.
Co-Authors - Yopi Abd. Rasyid Syamsuri ANDI UTAMA Andi Utama ANDO KATSUHIKO Apriliana, Pamella Ariani Hatmanti Ashadi Sasongko Caniago, Asnany Endang Saepudin, Endang Endang Sukara, Endang GINA KARTINA Herman Suryadi Herman Suryadi JAKA WIDADA Kusharyoto, Wien Kusumawati, Dinihari Indah Kusumawati, Dinihari Indah Lina Elfita Lina Elfita Meliah, Siti Meliah, Siti Mia Kusmiati MISA OTOGURO NANIK RAHMANI Nanik Rahmani Putri, Ade Lia Putri, Ade Lia ROHMATUSSOLIHAT ROHMATUSSOLIHAT RONI RIDWAN SHANTI RATNAKOMALA Shanti Ratnakomala Siti Meliah Subagus Wahyuono Sukma Nuswantara Susanti, Agustina Eko Tisnadjaja, Djadjat TOMOHIKO TAMURA Tutik Murniasih WIBOWO MANGUNWARDOYO Yantyati Widyastuti Yopi Yopi YOPI YOPI Yopi, YULIN LESTARI