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Journal : REKAYASA

Pengaruh Perbedaan Salinitas Terhadap Viabilitas Bakteri Pseudomonas spp. Arisandi, Apri; Wardhani, Maulinna Kusumo; Badami, Kaswan; Sopiyanti, Anisa
Rekayasa Vol 10, No 1: April 2017
Publisher : Universitas Trunojoyo Madura

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (612.678 KB) | DOI: 10.21107/rekayasa.v10i1.3600

Abstract

ABSTRAKKondisi perairan laut yang fluktuatif dan cenderung ekstrim menyebabkan rumput laut mudah terserang penyakit ice-ice. Munculnya bercak putih pada thallus rumput laut Eucheuma cottonii yang terinfeksi penyakit ice-ice diduga disebabkan oleh bakteri patogen yaitu Pseudomonas spp.  Tujuan yang ingin dicapai dalam pelaksanaan penelitian ini adalah untuk mengetahui pengaruh perbedaan salinitas terhadap viabilitas bakteri Pseudomonas spp dan untuk mengetahui pada salinitas berapakah bakteri Pseudomonas spp tidak dapat tumbuh. Bakteri diidentifikasi melalui uji biokimia, dan viabilitas diamati dengan menanam bakteri pada media TSA plate dengan salinitas 30, 32, dan 34 ppt dengan 3 kali pengulangan, dan untuk uji konfirmasi bakteri di tanam pada media TSA miring dengan kadar salinitas 0, 20, 40, 60, 80, dan 100 ppt. Hasil yang diperoleh menunjukan bakteri tumbuh dengan normal pada hampir semua media uji kecuali pada media 100 ppt, hal ini menunjukan bahwa bakteri Pseudomonas spp merupakan bakteri yang bersifat halofilik atau dapat tumbuh baik pada kadar salinitas yang tinggi. Kata Kunci: Eucheuma cottonii, ice-ice, Pseudomonosa spp,Viabilitas.  The Influence of Salinity Difference to Viability of Pseudomonas spp. BacteriaABSTRACTThe conditions of marine waters that fluctuate and tend to extremes can cause seaweed susceptible to ice-ice disease. The appearance of white patches on infected Eucheuma cottonii seaweed thallus is estimated as ice-ice disease which is caused by pathogenic bacteria of Pseudomonas spp  The objectives of this research is to determine the effect of salinity difference to viability of bacteria Pseudomonas spp and to know on what salinity is the bacterium Pseudomonas spp that can’t grow. Bacteria were identified by biochemical tests. Viability was observed by planting bacteria on TSA plate medium with salinity 30 ppt, 32 ppt, and 34 ppt with 3 repetitions, and for bacterial confirmation test in planting on TSA tilting with salinity 0 ppt, 20 ppt, 40 ppt, 60 ppt, 80 ppt, And 100 ppt. The results showed that bacteria grew normally in almost all test media except in 100 ppt media, this showed that Pseudomonas spp bacteria were halophilic bacteria or could grow well at high salinity levels.Keywords: Eucheuma cottonii, ice-ice, Pseudomonosa spp, Viability.
PENGARUH INDOL-3-BUTIRIC-ACID DAN THIDIAZURON TERHADAP MULTIPLIKASI TUNAS NENAS (Ananas comosus (L) Merr) CV. SMOOTH CAYYENE SECARA IN VITRO Syafii, Mohammad; Badami, Kaswan; Nursandi, Fatimah
Rekayasa Vol 6, No 1: April 2013
Publisher : Universitas Trunojoyo Madura

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (385.927 KB) | DOI: 10.21107/rekayasa.v6i1.2097

Abstract

Pineapple (Ananas comosus L.) is one of the most potential fruit crop cultivating in Indonesia. However, it is difficult to meet the demand for planting materials using the conventional propagation techniques due to production inefficiency. This research aim to know the influence solid medium of Murashige and Skoog (MS) composed of Indol-3-Butiric Acid and Thidiazuron, upon pineapple Var Smooth Cayenne shoot multiplication response. Shoot multiplication using a factorial complete random design. The first is a 3-level IBA concentration (0.3 ppm, 0.6 ppm, 0.9 ppm), a 3-level TDZ concentration (0.2 ppm, 0.4 ppm, 0.6 ppm). The finding shows that MS richly composed IBA and TDZ with low concentration gives 100% shoot the fastest periodic appearance (two weeks after incubation). Varians analysis finding shows that there is no interaction between IBA and TDZ.Low IBA concentration gives the higest quantity of shoot 9.32 (5.60 data transformation) at 5 week after incubation, at the end of the analysis tends to give the higest shoot 11.17 (6.18 data transformation) even though it is statistically not slighty difference. The highest TDZ concentration (0,6 ppm) give a few number of roots 0.89 (2,65 data transformation) and the lowes shoots leaf 3.84 (4.04 data transformation). The higher the TDZ is, the more nodal may appear.
Produksi Semangka Heterosis dengan Tetua Betina Jantan Mandul Triploid dan Jantan Hibrida Diploid Badami, Kaswan
Rekayasa Vol 1, No 1: April 2008
Publisher : Universitas Trunojoyo Madura

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1400.038 KB) | DOI: 10.21107/rekayasa.v1i1.2155

Abstract

Watermelon (Citrullus vulgaris, Schard) is a seasonal fruit playing an important role in economic improvement of a household and a nation as well. An effort to cultivate watermelon greatly supports the improvement offarmers' standard of living. Its Physical appearance and itsflesh color are greatly attractive. It has various stripped shape (elips, round and oval) and has highly enough nutrition.The aims of this stud y were to discover diploid watermelon varieties used as good polinator and has more strengths than both of its parents. This stud y applied Randomized Block Design with two replications treating nine (9) seeded watermelon and two (2) triploid verities. Thevarieties used as male parents were diploid variety (Pl), Diana Bangkok Dragon (P2), China Dragon (P3), Black sweet (P4), Golden Fresh (PS), Hitam manis (p6 ), Grand Master (pl), TM Tiger (PB), and Red Super Dragon (P9). Pretty Orchid (V1) and Champion (V2) were used asfemale parents.The results of this stud y showed that different polinators had different influences in determining the agronomic possibility of bearing fruit, sugar content,fruit length,fruit diameter except harvesting age and fruit weight. The best agronomic properties resulted from well crossing were shown byfruit diameter and sugar content. Withinvariety-crossing of all polinator with thefemale Pretty Orchid (V1) showed good results except the polinator Hitam Manis (p6 ). The greatest fruit diameter resulted from polinator Grand Master (Pl) was 22.59 cm dan the smallest resulted from Hitam Manis polinator (P6) was 20.20 cm
Identifikasi Kadar dan Pengaruh Sifat Kimia Tanah terhadap Metabolit Sekunder Kunyit (Curcuma domestiva Val.) di Bangkalan Sholehah, Diana Nurus; Amrullah, Arief; Badami, Kaswan
Rekayasa Vol 9, No 1: April 2016
Publisher : Universitas Trunojoyo Madura

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (254.752 KB) | DOI: 10.21107/rekayasa.v9i1.3336

Abstract

Efektivitas Isolat-Isolat Bacillus sebagai Pengendali Penyakit Bulai dan Pemacu Pertumbuhan Tanaman Jagung pada Kondisi Terkontrol Khoiri, Syaiful; Badami, Kaswan; Pawana, Gita; Utami, Ciwuk Sri
Rekayasa Vol 14, No 2: Agustus 2021
Publisher : Universitas Trunojoyo Madura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21107/rekayasa.v14i2.10270

Abstract

The main disease in maize is downy mildew caused by the fungus Peronosclerospora spp.. This pathogen can cause yield losses of up to 100%. Therefore, efforts to control this disease are continuously carried out, including technical culture, assembly of resistant plants, and use of synthetic fungicides. At the farm level, the use of metalaxyl, synthetic fungicides is the most common practice. On the other hand, it has been reported that some Peronosclerospora groups are starting to become resistant to metalaxyl. These problems lead to the need for alternative controls, for example with biological agents. Biological agents from bacterial groups have been developed to control plant disease, but for downy mildew is still limited. The purpose of this study is to screen and test Bacillus spp. ability to suppress downy mildew and promote the growth of maize. The assay was carried out on seeds by invitro to investigate growth-promoting reactions and also testing under controlled conditions in greenhouses to investigate the suppression ability of downy mildew disease development. The results showed B. polymyxa strain BP18, Bacillus subtilis strain BS41, Bacillus sp. strain BT1, and Bacillus sp. strains can stimulate the growth of corn seedlings and suppress downy mildew. The best isolate in suppressing downy mildew was Bacillus sp. strain BT1 with the smallest AUDPC value (3.94) and the highest protection index (82.71%). It is hoped that these results will find potential isolates and have the potential to be developed into biopesticides and biofertilizers.
Karakter Kuantitatif Melon Generasi S0-S4 Siti Rohmatin; Kaswan Badami; Gita Pawana
Rekayasa Vol 15, No 2: Agustus 2022
Publisher : Universitas Trunojoyo Madura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21107/rekayasa.v15i2.15109

Abstract

One of the obstacles to melon cultivation is the availability of seeds and the high price of melon seeds. This condition causes the need to assemble superior melon varieties with the expected characters. This study aims to form a pure line of melon with certain superior characteristics and can be proposed as a new high-yielding variety in the open-pollinated category. The research was conducted at the Experimental Garden of the Agrotechnology Study Program, Faculty of Agriculture, Trunojoyo University, Madura, from September-December 2022. This study used a randomized block design method. The treatments used were nine pure lines of melon (PK-669, D-162, PK-361, PK-165, PK-114, D-029, D-5089, PK-211, and comparison). Each treatment was repeated three times, and each experimental unit consisted of 12 plants. Research data were analyzed using analysis of variance (ANOVA) and HSD test α = 0.05. The selection advancement was analyzed by comparing character data for each generation. The research showed that the results of the characterization of ten melon lines S0-S4 generation on the characters of fruit length, fruit diameter, flesh thickness, and melon fruit weight showed a decreasing trend in each generation. The S4 generation (9.17 °brix) in the character of the average sugar content there was an increase that was not significantly different from the S3 generation (9.03 °brix), while the number of seeds in each generation had an average value that was not significantly different but occurred an increase in the number of seeds in the S3 (372) and S4 (381) generations. An increase in one generation of the population in the linear regression equation is estimated to reduce the character of fruit length by 0.50 cm, fruit diameter by 0.465 cm, flesh thickness by 0.140 cm, fruit weight by 0.199 Kg, sugar content by 0.752 °brix, and a number of seeds by 5.4.