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Optimasi Preparasi Sampel untuk Penetapan Kadar Protein Ekstrak Cacing Tanah (Lumbricus rubellus) Ahmad Fauzi; Wahyu Utami; Denny Vitasari; Arifah Sri Wahyuni
Jurnal Pharmascience Vol 9, No 1 (2022): Jurnal Pharmascience
Publisher : Program Studi Farmasi FMIPA Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/jps.v9i1.12961

Abstract

Demam tifoid merupakan salah satu penyakit yang menjangkit masyarakat Indonesia dengan 500 kasus per 100.000 penduduk dengan angka kematian mencapai 5%. Pengobatan utama demam tifoid adalah menggunakan berbagai macam antibiotik. Namun masyarakat Indonesia cenderung lebih memilih pengobatan alternatif atau tradisional. Salah satu obat tradisional demam tifoid adalah ekstrak cacing (Lumbricus rubellus) yang mempunyai kandungan protein sampai 71%. Kandungan protein sangat tergantung pada proses produksinya. Untuk itu diperlukan metode preparasi dan analisis untuk menentukan kadar protein yang bisa digunakan untuk menjamin mutu produk ekstrak cacing. Metode preparasi yang optimum  adalah dengan pemanasan pada temperatur 50°C selama 5 menit kemudian dianalisis kadar proteinnya dengan metode Lowry menggunakan spektrofotometer UV-Vis pada panjang gelombang 650,8 nm. Metode linier, akurat dan teliti dengan batas deteksi sebesar 0,18 mg/mL dan batas kuantifikasi sebesar 0,58 mg/mL. Kata Kunci: Protein, Cacing Tanah, Spektrofotometri, Metode Lowry  Typhoid fever is a disease that affects Indonesian people with 500 cases per 100,000 population with a mortality rate of 5%. The main treatment for typhoid fever is the use of various antibiotics. However, Indonesian people prefer to use alternative or traditional medicine. One of the traditional medicines for typhoid fever is earthworm (Lumbricus rubellus) extract which has a protein content of up to 71%. Protein content is highly dependent on the production process. For this reason, preparation and analysis methods are needed to determine protein levels that can be used to guarantee the quality of the worm extract products. The optimum preparation method is by heating at 50°C for 5 minutes and then the protein is analyzed using the Lowry's method using a UV-Vis spectrophotometer at wavelength of 650.8 nm. The method is linear, accurat and presice with  detection limit of 0.18 mg/mL and quantification limit of 0.58 mg/mL.
EVALUASI LOKASI TPA SAMPAH DI KABUPATEN KLATEN,INDONESIA DENGAN METODE TOPSIS Ahmad Fauzi; Eko Setiawan
Jurnal Ilmiah Teknik Industri Vol 9, No 1 (2021): Jurnal Ilmiah Teknik Industri : Jurnal Keilmuan Teknik dan Manajemen Industri
Publisher : Program Studi Teknik Industri, Fakultas Teknik Universitas Tarumanagara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24912/jitiuntar.v9i1.9457

Abstract

Penelitian ini bertujuan untuk mengevaluasi penentuan lokasi Tempat Pemrosesan Akhir (TPA) sampah di Kabupaten Klaten dengan mempertimbangkan beberapa kriteria yang relevan sehingga didapatkan lokasi yang sesuai dan optimal berdasarkan peraturan dan kebijakan pemerintah setempat. Peneliti menggunakan metode Technique For Order Preference By Similarity To Ideal Solution (TOPSIS) dengan mempertimbangkan kriteria berdasarkan peraturan SNI 03-3241-1994 dalam pengambilan keputusan. Penelitian ini mendapatkan enam calon lokasi alternatif yaitu Desa Dukuh Kecamatan Bayat, Desa Pakisan Kecamatan Cawas, Desa Troketon Kecamatan Pedan, Desa Ngolodono Kecamatan Karangdowo, Desa Kenaiban Kecamatan Juwiring, dan Desa Gunting Kecamatan Wonosari. Berdasarkan penelitian ini, Desa Troketon Kecamatan Pedan menempati peringkat tertinggi sebagai lokasi TPA sampah di Kabupaten Klaten. Penelitian yang sama juga menemukan bahwa Desa Pakisan di Kecamatan Cawas merupakan alternatif lokasi yang menempati peringkat terbawah sebagai alternatif lokasi TPA sampah di Kabupaten Klaten.
Uji Aktivitas Antikolesterol Kombinasi Ekstrak Teh Oolong, Buncis Dan Kayu Manis Ahmad Fauzi; Tanti Azizah Sujono; Azis Saifudin; Arifah Sri Wahyuni
Pharmacon: Jurnal Farmasi Indonesia Vol 19, No 1 (2022)
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/pharmacon.v19i1.18193

Abstract

Hyperlipidemia has an important role in the occurrence of cardiovascular, cerebrovascular, and peripheral vascular diseases. High cholesterol and LDL levels in the blood are at risk of causing various diseases such as atherosclerosis, pancreatic dysfunction and kidney disorders. Meanwhile, high triglyceride levels can cause impaired liver, pancreas and kidney function. Green bean, oolong tea and cinnamon are plants that can lower cholesterol levels. Oolong tea contains catechins, Green Bean contain phytosterols and cinnamon contains cinnamaldehyde. Determination of secondary metabolite levels of extracts was carried out using the Liquid Chromatography – Mass Spectroscopy (LC-MS) method. The activity test was carried out with 30 Wistar white rats which were divided into 6 test groups. The normal control group was treated with CMC-Na without feeding hypercholesterolemia, the positive control group was treated with simvastatin 0.4 mg/KgBW, the negative control group was treated with CMC-Na, the test groups 1, 2 and 3 were treated with cinnamon extract, oolong tea and green bean, respectively (150:50:50 mg/KgBW), (50:150:50 mg/KgBW) and (50:50:150 mg/KgBW). The detected metabolite content was cinnamaldehyde in cinnamon up to 10%, catechins in oolong tea up to 12% and stigmasterol in beans as much as 3.4%.The results obtained by test group 1 and test group 2 can reduce cholesterol and triglyceride levels (p0.05). All test groups had no significant effect on HDL and LDL levels.
Measurement of Protein Content in Extract of Earthworm Ahmad Fauzi; Wahyu Utami; Denny Vitasari; Arifah Sri Wahyuni
Urecol Journal. Part C: Health Sciences Vol. 2 No. 1 (2022): January-June
Publisher : Konsorsium LPPM Perguruan Tinggi Muhammadiyah 'Aisyiyah (PTMA) Koordinator Wilayah Jawa Tengah - DIY

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (300.905 KB) | DOI: 10.53017/ujhs.136

Abstract

Earthworms are widely used by Indonesian people as an alternative treatment of typhoid fever in addition to the main treatment using antibiotics. One of the main ingredients of earthworms (Lumbricus rubellus) is protein. The amount of protein content of the extracts is highly dependent on the production process. For this reason, a spectrophotometric analysis was carried out to determine the protein content of the worm extracts in the market. As a result of the analysis using the Lowry method at a maximum wavelength of 650.8 nm, the protein content of a sample of earthworm extract obtained from the market was (10.41 ± 0.47) %.
EVALUASI LOKASI TPA SAMPAH DI KABUPATEN KLATEN,INDONESIA DENGAN METODE TOPSIS Ahmad Fauzi; Eko Setiawan
Jurnal Ilmiah Teknik Industri Vol. 9 No. 1 (2021): Jurnal Ilmiah Teknik Industri : Jurnal Keilmuan Teknik dan Manajemen Industri
Publisher : Program Studi Teknik Industri, Fakultas Teknik Universitas Tarumanagara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24912/jitiuntar.v9i1.9457

Abstract

Penelitian ini bertujuan untuk mengevaluasi penentuan lokasi Tempat Pemrosesan Akhir (TPA) sampah di Kabupaten Klaten dengan mempertimbangkan beberapa kriteria yang relevan sehingga didapatkan lokasi yang sesuai dan optimal berdasarkan peraturan dan kebijakan pemerintah setempat. Peneliti menggunakan metode Technique For Order Preference By Similarity To Ideal Solution (TOPSIS) dengan mempertimbangkan kriteria berdasarkan peraturan SNI 03-3241-1994 dalam pengambilan keputusan. Penelitian ini mendapatkan enam calon lokasi alternatif yaitu Desa Dukuh Kecamatan Bayat, Desa Pakisan Kecamatan Cawas, Desa Troketon Kecamatan Pedan, Desa Ngolodono Kecamatan Karangdowo, Desa Kenaiban Kecamatan Juwiring, dan Desa Gunting Kecamatan Wonosari. Berdasarkan penelitian ini, Desa Troketon Kecamatan Pedan menempati peringkat tertinggi sebagai lokasi TPA sampah di Kabupaten Klaten. Penelitian yang sama juga menemukan bahwa Desa Pakisan di Kecamatan Cawas merupakan alternatif lokasi yang menempati peringkat terbawah sebagai alternatif lokasi TPA sampah di Kabupaten Klaten.
Implementasi Evaluasi Pembelajaran Pendidikan Al Islam di Sekolah Menengah Atas Muhammadiyah Ahmad Fauzi; Nurul Latifatul Inayati
Munaddhomah: Jurnal Manajemen Pendidikan Islam Vol. 4 No. 2 (2023): Management of Islamic Education
Publisher : Prodi Manajemen Pendidikan Islam Pascasarjana Institut Pesantren KH. Abdul Chalim Mojokerto

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31538/munaddhomah.v4i2.438

Abstract

The purpose of this study is to identify the basic ideas of evaluation and their consequences for the assessment of Islamic religious education taught in schools. Qualitative techniques and literature review methods were used in this work. The findings of the discussion show that measurement and assessment shape evaluation. Because it is included in the learning phase, evaluation plays an important strategic role in the learning environment. The aim is to evaluate the efficacy and efficiency of the learning system. Assessment of learning programs, learning activities, and learning outcomes are included in the discussion area. Continuity, completeness, fairness, objectivity, collaboration, and practicality are some of the common evaluation concepts. Its principles consist of responsibility, pedagogy, integration, and coherence, to be precise. Planning, development, monitoring, efficiency, effectiveness, and a comprehensive program are all evaluated as part of the learning process. According to the object, input, transformation, and output are all included in the learning evaluation. Trainers, trained police, and self-evaluating students are among the topics covered. It consists of tests and non-tests, technically speaking. Examination of Islamic religious subjects in schools must be carried out regularly, comprehensively, and holistically. As a result, Islamic religion teachers must be able to evaluate the progress of their students in Aqliyah, Qolbiyah, and Amaliyah.
Measurement of Protein Content in Extract of Earthworm Ahmad Fauzi; Wahyu Utami; Denny Vitasari; Arifah Sri Wahyuni
Urecol Journal. Part C: Health Sciences Vol. 2 No. 1 (2022): January-June
Publisher : Konsorsium LPPM Perguruan Tinggi Muhammadiyah 'Aisyiyah (PTMA) Koordinator Wilayah Jawa Tengah - DIY

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53017/ujhs.136

Abstract

Earthworms are widely used by Indonesian people as an alternative treatment of typhoid fever in addition to the main treatment using antibiotics. One of the main ingredients of earthworms (Lumbricus rubellus) is protein. The amount of protein content of the extracts is highly dependent on the production process. For this reason, a spectrophotometric analysis was carried out to determine the protein content of the worm extracts in the market. As a result of the analysis using the Lowry method at a maximum wavelength of 650.8 nm, the protein content of a sample of earthworm extract obtained from the market was (10.41 ± 0.47) %.