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All Journal Proceedings of Annual International Conference Syiah Kuala University - Life Sciences & Engineering Chapter Jurnal Medika Veterinaria
Zakiah Heryawati Manaf
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Engineering Immunology & microbiology Medicine & Pharmacology Veterinary Other

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Isolation of gram-negative bacteria from metacarpal injury of Panthera tigris sumatrae trapped in Subulussalam, Indonesia . Darmawi; . Darniati; Zakiah Heryawati Manaf; . Syafruddin; Arman Sayuti
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 1, No 1 (2011): Life Sciences
Publisher : Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (92.797 KB)

Abstract

The present study aimed to isolate gram-negative bacteria infected metacarpal of Panthera tigris sumatrae trapped in Subulussalam, Aceh Province. Swab sample was obtained from metacarpal injury of P. tigris sumatrae. Swab sample was cultured to nutrient broth media using sterile cotton swabs or Pasteur pipettes, and incubated at 37°C temperature for 24 hours. Culture was spared on MacConkey media and incubated again at 37°C temperature for 24 hours. Determination of bacteria colony growth on the surface of MacConkey media based on shape, colour, surface, size, and viscosity (consistency). The bacteria colony stained with Gram staining, and tested biochemically.  The result showed that gram-negative bacteria such as Citrobacter sp., Proteus sp., Providencia sp., Pseudomonas sp.,and Salmonella sp. isolated from metacarpal injury of P. tigris sumatrae.
AKURASI PERKIRAAN POPULASI ANJING LIAR SEBAGAI RISIKO HEWAN PENULAR RABIES DI EMPAT KECAMATAN KOTA BANDA ACEH MENGGUNAKAN SOFTWARE APSP (The Estimation Accuracy of Stray Dogs Population as a Risk of Rabies Transmission in Four Sub-District of Banda Aceh Using APSP Software) Syehaffer Wahyudi Affandi; Teuku Reza Ferasyi; Nurliana Nurliana; Zakiah Heryawati Manaf; Sulasmi Sulasmi; Razali Razali
Jurnal Medika Veterinaria Vol 9, No 2 (2015): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v9i2.3814

Abstract

This study aimed to test the estimation accuracy of stray dogs population using video camera equipped with software of animal perception of seismic phenomena (APSP) and manually observation which counting the number of biting cases by rabies-risk animals (RRA) in four sub-districts of Banda Aceh (grouped as highest and lowest reported biting cases). This study was conducted by direct observation and counting the number of stray dogs around rubbish bin at night (10.00-12.00 pm) in each sub-district, the counting was repeated 3 times at the same time on different day. The data was analyzed using world society for the protection of animals (WSPA) methods. Overall, the results showed that the number of stray dogs around rubbish bin in four sub-districts were highest based on manual counting method as compared to counting by using video camera equipped with APSP software. The numbers of stray dogs were high in the sub-districts with higher reported bitting cases by RRA. As conclusion, counting of stray dogs by using manual methods is still more reliable than using video camera equipped with APSP software.Key words: rabies, stray dogs, population, software APSP
DETEKSI ANTIBODI SERUM AYAM KAMPUNG (Gallus domesticus) TERHADAP VIRUS NEWCASTLE DISEASE DI KOTA BANDA ACEH Darmawi -; Fakhrurrazi -; Wiliana -; Maryulia Dewi; Mahdi Abrar; Faisal Jamin; Zakiah Heryawati Manaf
Jurnal Medika Veterinaria Vol 9, No 1 (2015): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v9i1.2983

Abstract

Penelitian ini bertujuan mengetahui titer antibodi pada serum ayam kampung (Gallus domesticus) terhadap virus Newcastle disease (ND). Sampel penelitian adalah serum ayam kampung yang diperoleh di beberapa pasar tradisional Banda Aceh. Parameter yang diamati dalam penelitian ini adalah titer antibodi serum ayam kampung dari uji hemaglutinasi inhibisi (HI) dengan memperhatikan endapan eritrosit. Data hasil pengamatan dianalisis secara deskriptif. Hasil penelitian menunjukkan bahwa dari 120 sampel yang diambil di beberapa pasar tradisional di kota Banda Aceh hanya 15 sampel (12,5%) menunjukkan protektif sedangkan 105 sampel (87,5%) tidak protektif. Tingkat proteksi ayam terhadap virus ND di pasar tradisional di kota Banda Aceh tergolong sangat rendah.
DAYA HAMBAT GETAH JARAK CINA (Jatropha multifida L.) TERHADAP Staphylococcus aureus SECARA IN VITRO Darmawi -; Zakiah Heryawati Manaf; Fahri Putranda
Jurnal Medika Veterinaria Vol 7, No 2 (2013): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v7i2.2946

Abstract

Penelitian ini bertujuan mengetahui pengaruh getah jarak cina (Jatropha multifida L.) terhadap pertumbuhan bakteri Staphylococcus aureus. Sampel yang digunakan dalam penelitian ini adalah Stapylococcus aureus dengan no Cat ATCC 25923 diperoleh dari Laboratorium Mikrobiologi Fakultas Kedokteran Hewan Universitas Syiah Kuala. Getah  jarak cina diperoleh dari Desa Rukoh Kecamatan Syiah Kuala,diambil dengan cara menyayat batang jarak dan ditampung dalam tabung reaksi (Pyrex TM) sebanyak 50 ml. Penelitian ini dilakukan dengan 3 kali ulangan menggunakan konsentrasi 25; 50; 75; dan 100% v/v getah jarak cina. Kontrol positif digunakan kertas cakram antibiotik Vankomisin 30 µg. Daya hambat getah jarak cina menggunakan metode Kirby Bauer, dengan jumlah bakteri sesuai dengan standar Mc Farland I. Data yang diperoleh dianalisis secara deskriptif. Zona hambat yang terbentuk diukur dengan jangka sorong (Caliver TM). Hasil penelitian pada konsentrasi 25; 50; 75; dan 100% v/v zona hambat yang terbentuk masing-masing adalah 13; 13,5; 14,6;  dan 15,7 mm. Zona hambat yang terbentuk dariantibiotik vankomisin 30 µg adalah 21 mm. Dari hasil penelitian dapat di simpulkan bahwa getah jarak cina (Jatropha multifida L.) dapat menghambat pertumbuhan Staphylococcus aureus.
Co-Authors . Darmawi . Darniati . Syafruddin Arman Sayuti Darmawi - Fahri Putranda Faisal Jamin Fakhrurrazi - Mahdi Abrar Mahdi Abrar Maryulia Dewi NURLIANA NURLIANA Razali Razali Sulasmi Sulasmi Syehaffer Wahyudi Affandi Teuku Reza Ferasyi Teuku Reza Ferasyi Wiliana -