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All Journal HAYATI Journal of Biosciences Jurnal Ilmu Pertanian Indonesia Jurnal Fitopatologi Indonesia Microbiology Indonesia BIOTROPIA - The Southeast Asian Journal of Tropical Biology AL KAUNIYAH JSDH Jurnal Biologi Tropis Makara Journal of Science
Sri Listiyowati
Departmen Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, IPB University, Kampus IPB Darmaga, Bogor 16680
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Veterinary

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Journal : JSDH

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Potensi Cendawan Asal Hydrilla verticillata Sebagai Pengendali Hayati Fusarium oxysporum dan Ganoderma boninense: Fungi from Hydrilla verticillata as Potential Biological Control Agents of Fusarium oxysporum and Ganoderma boninense Astri Ariyani; Nampiah Sukarno; Sri Listiyowati
Jurnal Sumberdaya Hayati Vol. 5 No. 2 (2019)
Publisher : Departemen Biologi, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.5.2.43-51

Abstract

Hydrilla verticillata is an aquatic weed, can build symbiosis and also become a substrate of freshwater fungi. Some of freshwater fungi that live in association with H. verticillata are pathogenic fungi which were potentially used as controller for Fusarium oxysporum and Ganoderma boninense. The use of pathogenic fungi as a biocontrol is not consistent in the field. Therefore, this research aimed to find new fungus candidates that are stronger and more consistent in controlling pathogenic fungi. Freshwater fungi were isolated from H. verticillata. Identification was based on morphological characters. Molecular analysis was carried out for freshwater fungi which had the highest inhibition percentage to pathogenic fungi. The antagonism test was conducted by dual culture method. The mechanism of interaction of hyphae was observed under a microscope. Freshwater fungi was derived from H. verticillata stem are Aspergillus section flavi, Gliomastix sp., and Talaromyces sp. Antagonism test results showed that all fungi could inhibit the growth of pathogenic fungi. Based on molecular analysis used ITS region showed Talaromyces sp. could not identify to the level of species so that additional genes were required, namely beta-tubulin gene, in order to further identify Talaromyces sp.
Isolasi Cendawan Mikoriza Arbuskula dari Rizosfer Tanaman Berkayu Asal Pulau Bangka dan Karakteristik Struktur Kultur Mikorizanya: Isolation of Arbuscular Mycorrhizal Fungi from Rhizosphere of Bangka Island Woody Plants and their Mycorrhizal Structure Culture Characteristics Nampiah Sukarno; Cici Rahmawati; Sri Listiyowati; Wendi Nurul Fadillah; Yanti Novera
Jurnal Sumberdaya Hayati Vol. 9 No. 2 (2023)
Publisher : Departemen Biologi, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.9.2.39-48

Abstract

Arbuscular Mycorrhizal Fungi (AMF) associate with a variety of plants including forest trees. Research on AMF in forestry in Indonesia is limited, especially on woody plants grown in Bangka Island. Therefore, the aim of this research was to isolate and identify AMF associated with woody plants from Bangka Island grown in the post-mining soil in greenhouse for 7 years. The Soil samples derived from 8 pots of the 7 years old woody plants were used as spore sources. Spore extraction from the soil used the wet sieving and decanting method. Fungal identification was carried out based on morphological characteristics, and fungal isolation used pot culture with Pueraria javanica as a host plant. Fungal structures within the root were analyzed after root staining with trypan blue. There were 18 spore morphotypes observed which belong to 5 types of Acaulospora and 13 types of Glomus. Seven single spore pot cultures were successfully isolated from species of Acaulospora sp.1, Acaulospora sp.4, Acaulospora sp.5, Glomus sp.2, Glomus sp.3, Glomus sp.6, and Glomus sp.11. AM fungal colonization structures observed within the root were Paris type arbuscules, internal hyphae, and vesicles. The AMF cultures obtained could be used as biofertilizer for woody plant seedlings production for post mining reclamation activities.
Co-Authors ALEX HARTANA Alex Hartana Astri Ariyani Cici Rahmawati Delfiani Anggias Putri Firda Sri Efendi Firyal Noviatanti Nabilah Gayuh Rahayu Gayuh Rahayu Handika Dwi Prasetyo Ira Zahara Nurwulansari Irmanida Batubara Irmanida Batubara Kemala S. Nagur Lenka, Audria Bayu Maulida Aqlinia MUHAMMAD JUSUF Muhammad Jusuf Nadila Fany Ashafani Nampiah Sukarno Nampiah Sukarno Okky Setyawati Dharmaputra Rika Indri Astuti Rika Indri Astuti Rika Indri Astuti Tya Rustiani UTUT WIDYASTUTI UTUT WIDYASTUTI Vincentia Fenice Angger Maherani Wahyu Aji Mahardhika Wendi Nurul Fadillah Yanti Novera