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All Journal HAYATI Journal of Biosciences Jurnal Ilmu Pertanian Indonesia Jurnal Fitopatologi Indonesia Microbiology Indonesia BIOTROPIA - The Southeast Asian Journal of Tropical Biology AL KAUNIYAH JSDH Jurnal Biologi Tropis Makara Journal of Science
Sri Listiyowati
Departmen Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, IPB University, Kampus IPB Darmaga, Bogor 16680
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Veterinary

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Journal : BIOTROPIA - The Southeast Asian Journal of Tropical Biology

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Identification of Aspergillus flavus and Detection of Its Aflatoxin Genes Isolated from Peanut and Peanut Processed Products Kemala S. Nagur; Nampiah Sukarno; Sri Listiyowati
BIOTROPIA - The Southeast Asian Journal of Tropical Biology Vol. 21 No. 1 (2014)
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (164.864 KB) | DOI: 10.11598/btb.2014.21.1.398

Abstract

Aspergillus flavus is one of the main fungi that are able to produce aflatoxin.The presence of the fungi and its aflatoxin are become serious problem on food safety. This research was aimed to isolate and identify A. flavus from peanut and its processed products collected from some traditional markets in Bogor, Depok and Jakarta, and detection of their aflatoxin genes. Fungal isolation was using AFPA media. Fungal identification was carried out by combining morphological and molecular analysis used species specific primers FVAVIQ1/FLAQ2 and AFLA-F/AFLA-R, while detection of  aflatoxin genes employed four specific primers of apa-2 (aflR), nor-1 (aflD), ver-1 (aflM) and omt-1 (aflP). From 36 samples, the A. flavus group was only found in peanut kernels samples with viable count of specific colonies in the range of 0.01-5.52 x 10-4 cfu/g. The total 18 isolates were identified as A. flavus based on species specific primers FVAVIQ1/FLAQ2 and AFLA-F/AFLA-R by producing amplicons about 100 and 413 bp respectively. Based on aflatoxin gene analysis showed that all 18 isolates successfully amplified by both apa-2 and nor-1, 83.3 % by omt-1 and 72.2 % by ver-1 genes which taking part in aflatoxin production. The amplicons size of apa-2, nor-1, ver-1 and omt-1   primer pairs were about 1032, 400, 895 and 1024 bp respectively.
Co-Authors Alex Hartana ALEX HARTANA Astri Ariyani Cici Rahmawati Delfiani Anggias Putri Firda Sri Efendi Firyal Noviatanti Nabilah Gayuh Rahayu Gayuh Rahayu Handika Dwi Prasetyo Ira Zahara Nurwulansari Irmanida Batubara Irmanida Batubara Kemala S. Nagur Lenka, Audria Bayu Maulida Aqlinia Muhammad Jusuf MUHAMMAD JUSUF Nadila Fany Ashafani Nampiah Sukarno Nampiah Sukarno Okky Setyawati Dharmaputra Rika Indri Astuti Rika Indri Astuti Rika Indri Astuti Tya Rustiani UTUT WIDYASTUTI UTUT WIDYASTUTI Vincentia Fenice Angger Maherani Wahyu Aji Mahardhika Wendi Nurul Fadillah Yanti Novera