Sri Kunarti
Department Of Conservative Dentistry, Faculty Of Dental Medicine, Universitas Airlangga

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The application of lesion sterilization and tissue repair 3Mix-MP for treating rat's dental pulp tissue Raditya Nugroho; Ananta Tantri Budi; Sri Kunarti
Dental Journal (Majalah Kedokteran Gigi) Vol. 48 No. 1 (2015): March 2015
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (292.447 KB) | DOI: 10.20473/j.djmkg.v48.i1.p12-15

Abstract

Background: Lesion sterilization and tissue repair (LSTR) 3Mix-MP are three broad-spectrum antibiotics, including metronidazole, ciprofloxacin and minocycline are mixed with propylene glycol or macrogol. There is the possibility ofthe healing process that marked proliferation ofnew blood vessels and proliferation offibroblasts in the treatment ofirreversible pulpitis by pulp capping LSTR 3MixMP because of  the principle of the method LSTR 3Mix-MP is to kill bacteria. Purpose: The purpose of this study to prove the effect of LSTR 3Mix-MP on chronic inflammation and the healing process in rat dental pulp tissue in vivo. Methods: Rattus norvegicus anaesthetized by using ketamine and xylazine dissolved in sterile isotonic saline solution (0.2 ml/50gr mm) on the upper right thigh. Cavity preparation class I to perforation by using a low speed tapered diamond round bur. In the treatment group, rats were treated 3Mix-MP at a dose of10 mg and then covered with glass ionomer cement for 7 days on the pulp that has been opened for 3 days. The control group treated with saline irrigation on the pulp that has been opened for 3 days. Rats were killed after seven days, and then made preparations pulp tissue to count the number oflymphocytes, macrophages, plasma cells, blood vessels, and fibroblasts Results: There is an increase in the average number ofmacrophage cells, plasma, and fibroblasts; and decreased lymphocytes and blood vessels in the treated group exposure LSTR 3Mix-MP. Conclusion:LSTR 3Mix-MP can reduce chronic inflammation process and enhance the healing process in rat dental pulp tissue.
Genotoxicity test of propolis extract, mineral trioksida aggregat, and calcium hydroxide on fibroblast BHK-21 cell cultures Ceples Dian Kartika W.P; Sri Kunarti; Ari Subiyanto
Dental Journal (Majalah Kedokteran Gigi) Vol. 48 No. 1 (2015): March 2015
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (970.429 KB) | DOI: 10.20473/j.djmkg.v48.i1.p16-21

Abstract

Background: Health industry has always used natural products as an alternative. Propolis, a natural antibiotic, is a resinous yellow brown or dark brown substance derived from honey bees (Apis mellifera). The main chemical compounds contained in propolis are flavonoids, phenolics and other various aromatic compounds. Flavonoids are well known plant compounds that have antibacterial, antifungal, antiviral, antioxidant and anti-inflammatory proprieties. Propolis is expected to be an alternative used for root canal treatment with lower toxicity compared to calcium hydroxide (Ca(OH)2 . Over the last decade, a new material, mineral trioxide aggregate (MTA) was developed, and has been used as the gold standard. All materials used in mouth should be biocompatible. The initial level of material biocompatibility evaluation involves toxicity and genotoxicity tests. Purpose: This research is aimed to conduct comparison test of genotoxicity effect of propolis extract, MTA and Ca(OH)2 on fibroblast BHK-21 cell culture. Methods: This research was conducted with single-cell gel electrophoresis method. Results: The results indicate that propolis extract cannot cause DNA damage, while MTA can cause apoptosis and Ca(OH)2 can cause neucrosis. Conclusion: It can be concluded that propolis extract has genotoxicity effect lower than MTA and Ca(OH)2 , but MTA has lower effect on fibroblast BHK-21 cell culture.
Stimulation of type I collagen activity in healing of pulp perforation Sri Kunarti
Dental Journal (Majalah Kedokteran Gigi) Vol. 41 No. 4 (2008): December 2008
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (385.832 KB) | DOI: 10.20473/j.djmkg.v41.i4.p182-185

Abstract

Background: TGF-β1 is a connective tissue stimulant, potential regulator for tissue repair, and promoter in wound healing. The healing of pulp perforation is decided by quantity and quality of new collagen deposition. TGF-β1 upregulates collagen transcription. However, after several weeks production of type I collagen synthesis is stopped and enzymatic degradation of collagen matrix will occur. Purpose: Observe synthesis type I collagen during the process of pulp perforation healing in 7, 14, and 21 days after being treated using TGF-β1. Methods: This research in vivo from orthodontic patients indicated for premolar extraction, between ages 10–15 years. A class V cavity preparation was created in the buccal aspect 1 mm above gingival margin until pulp exposure. Cavity was irrigated slowly with saline solution and dried with a sterile small cotton pellet. The sterile absorbable collagen membrane used, soaked with 5 μl of TGF-β1. It was covered by teflon pledge to separate from glass ionomer cement as restoration. Evaluated on day 7th, 14th, and 21st. All samples were extracted and prepared for immunohystochemical examination. Result: Data were analyzed by t-test. There was significant difference in synthesis type I collagen compared between 7–14 days and 7–21 days after treatment by TGF-β1 also Ca(OH)2. There was significant difference compared between TGF-β1 and Ca(OH)2 in 14–21 days after treatment (means TGF-β1 > Ca(OH)2). Conclusion: Elevation of synthesis type 1 collagen by TGF-β1 in 20 mg/ml.
Pulp tissue inflammation and angiogenesis after pulp capping with transforming growth factor β1 Sri Kunarti
Dental Journal (Majalah Kedokteran Gigi) Vol. 41 No. 2 (2008): June 2008
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (313.845 KB) | DOI: 10.20473/j.djmkg.v41.i2.p88-90

Abstract

In Restorative dentistry the opportunity to develop biomemitic approaches has been signalled by the possible use of various biological macromolecules in direct pulp capping reparation. The presence of growth factors in dentin matrix and the putative role indicating odontoblast differentiation during embryogenesis has led to the examination on the effect of endogenous TGF-β1. TGF-β1 is one of the Growth Factors that plays an important role in pulp healing. The application of exogenous TGF-β1 in direct pulp capping treatment should be experimented in fibroblast tissue in-vivo to see the responses of inflammatory cells and development of new blood vessels. The increase in food supplies always occurs in the process of inflammation therefore the development of angiogenesis is required to fulfil the requirement. This in-vivo study done on orthodontic patients indicated for premolar extraction between 10–15 years of age. A class V cavity preparation was created in the buccal aspect 1 mm above gingival margin to pulp exposure. The cavity was slowly irrigated with saline solution and dried using a sterile small cotton pellet. The sterile absorbable collagen membrane was applied and soaked in 5 ml TGF-β1. It was covered by a Teflon pledge to separate from Glass Ionomer Cement restoration. Evaluation was performed on day 7; 14; and 21. All samples were histopathologycally examined and data was statistically analysed using one way ANOVA and Dunnet T3.There were no inflammatory symptoms in clinical examination on both Ca(OH)2 and TGF-β1, but they increased the infiltration of inflammatory cells on histopathological examination. There were no significant differences (p > 0.05) between Ca(OH)2 and TGF-β1 in inflammation cell and significant differences (p < 0.05) in angiogenesis on day 7 and 14. There were no significant differences (p > 0.05) in inflammation cell with in TGF-β1 groups and significant differences (p < 0.05) with in Ca(OH)2 groups on day 7 and 14. It is concluded TGF-β1 functions as direct capping medication has the same inflammatory response as Ca(OH)2, however, TGF-β1 developed angiogenesis earlier than Ca(OH)2.
Pulp tissue vacuolization and necrosis after direct pulp capping with calcium hydroxide and transforming growth factor-β1 Sri Kunarti
Dental Journal (Majalah Kedokteran Gigi) Vol. 41 No. 1 (2008): March 2008
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (400.923 KB) | DOI: 10.20473/j.djmkg.v41.i1.p35-38

Abstract

Mechanical pulp exposure by a rotary cutting instrument or a hand-cutting instrument often happens in deep caries. Application of protective dressing can protect the pulp from additional injury by facilitating healing and repair. Pulp capping has been suggested as one treatment of choice after pulp exposure to maintain pulp vitality. TGF-β1 is growth factor that has important rule in wound healing. The application of Ca(OH)2 and exogenous TGF-β1 as direct pulp capping tr4eatment must be experimented in-vivo to see the vacuolization and necrosis in 7, 14, and 21 days after application. This research was done in vivo experiment from orthodontic patients indicated for premolar extraction, between ages 10–15 years. A class V cavity preparation was created in the buccal aspect 1 mm above gingival margin until pulp exposure. Cavity was irrigated slowly with saline solution and dried with a sterile small cotton pellet. Group 1 calcium hydroxide was applied as manufacture procedure. Group 2, the sterile absorbable collagen membrane used, as inert carrier of TGF-β1 was soaked with 5 ml. All groups were covered by a Teflon pledge to separate pulp capping agent from glass ionomer cement restoration. Teeth extracted in 7, 14 and 21 days after treatment. All samples were hystopathologically examined. There were significant difference of TGF-β1 (p < 0.05) in the vacuolization day 14th and 21th compared with 7th. there were not significant difference in necrosis for all variables. Vacuolization and necrosis decreased in the application of TGF-β1.
The effect of acidulated phosphate fluoride application on dental enamel surfaces hardness Edhie Arief P; Sri Kunarti
Dental Journal (Majalah Kedokteran Gigi) Vol. 40 No. 3 (2007): September 2007
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (146.947 KB) | DOI: 10.20473/j.djmkg.v40.i3.p144-147

Abstract

Enamel demineralization by acid is the first step of caries process. It has recently been shown that acidulated phosphate fluoride (APF) can maintain the hardness of enamel surface. The aim of this study was examine the effect of APF application in the hardest of enamel surface. Fifty extracted teeth were cut at their crown, 40 teeth were taken randomly then divided into 4 groups, group 1 as the control, group 2 was treated with APF for 1 minute, group 3 for 4 minutes and group 4 for 7 minutes, then all the samples were washed with demineralized water. To see the effect of APF, all of the samples were soaked in lactic acid demineralization solution with pH 4,5 for 72 hours., the hardness of the surfaces of those samples before and after the treatment was measured by Micro Vickers Hardness Tester. The data were analyzed using One-Way ANOVA and LSD tests. In conclusion, 1.23% APF gel can reduce higher enamel demineralization.
The thickness of odontoblast-like cell layer after induced by propolis extract and calcium hydroxide Irfan Dwiandhono; Ruslan Effendy; Sri Kunarti
Dental Journal (Majalah Kedokteran Gigi) Vol. 49 No. 1 (2016): March 2016
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (404.702 KB) | DOI: 10.20473/j.djmkg.v49.i1.p17-21

Abstract

Background: Propolis is a substance made from resin collected by bees (Apis mellifera) from variety of plants, mixed with its saliva and various enzymes to build a nest. Propolis has potential antimicrobial and antiinflammatory agents with some advantages over calcium hydroxide (Ca(OH)2). Ca(OH)2 has been considered as the “gold standard” of direct pulp-capping materials, but there are still some weakness of its application. First, it can induce pulp inflammation which last up to 3 months. Second, the tissue response to Ca(OH)2 is not always predictable. Third, the tunnel defect can probably formed in dentinal bridge with possible bacterial invasion in that gap. Purpose: This study was aimed to determine and compare the thickness of odontoblast-like cells layer after induced by propolis extract and Ca(OH)2 in rat’s pulp tissue. Method: Class 1 preparation was done in maxillary first molar tooth of wistar mice until the pulp opened. The Ca(OH)2 and propolis extract was applied to induce the formation of odontoblast-like cells, the cavity was filled with RMGIC. The teeth were extracted (after 14 and 28 days of induction). The samples were then processed for histological evaluation. Result: There were significant differences between the thickness of odontoblast-like cells after induced by propolis extract and Ca(OH)2. Conclusion: The propolis extract as the direct pulp capping agent produces thicker odontoblast-like cell layer compared to Ca(OH)2.
Proliferation of odontoblast-like cells following application of a combination of calcium hydroxide and propolis Ira Widjiastuti; Sri Kunarti; Fauziah Diajeng Retnaningsih; Evri Kusumah Ningtyas; Debby Fauziah Suryani; Andrie Handy Kusuma
Dental Journal (Majalah Kedokteran Gigi) Vol. 52 No. 4 (2019): December 2019
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v52.i4.p183-186

Abstract

Background: One purpose of operative dentistry is the maintenance of healthy pulp by reducing the need for root canal treatment and the possibility of undesirable scenarios such as tooth loss. Propolis is a plant-derived substance that contains a resin produced by honeybees belonging to the Apis mellifera species. Purpose: This study aimed to investigate the effect of a combination of calcium hydroxide (Ca(OH)2) and propolis extract on odontoblast-like cell proliferation in Wistar rats (Rattus norvegicus). Methods: This research constituted a true experimental laboratory-based investigation with post-test control group design. Thirty Wistar rats were randomly divided into six groups. The first molar pulp of each sample was perforated on occlusal surfaces using a low speed round bur. On day 3, the samples were divided into six groups (n=10): Group I: control; Group II: Ca(OH)2 + 11%; propolis extract; Group III: Ca(OH)2 + aquadest, and on day 7: Group IV: control; Group V: Ca(OH)2 + 11% propolis extract; Group VI: Ca(OH)2 + aquadest. All samples were filled with restorative material. They were subsequently sacrificed after 3 and 7 days post-pulp capping administration and the afflicted tooth extracted for hematoxylin and eosin (H&E) staining. The resulting data was subjected to statistical analysis to ascertain the proliferation of odontoblast-like cells. The significance of differences between the groups was determined by a one-way ANOVA test followed by a post hoc Tuckey HSD. A p-value <0.05 was considered to be significant. Results: On day 3, a significant difference existed between group II (Ca(OH)2–propolis) and group I (control group) and group III (Ca(OH)2–aquades), whereas Ca(OH)2–propolis revealed that the proliferation of odontoblast-like cells was higher. Meanwhile, on day 7, there was a significant difference between all groups whereas, with regard to Ca(OH)2–propolis, the proliferation of odontoblast-like cells in group V was higher. Conclusion: Application of combination of Ca(OH)2-propolis extract can increase the proliferation of odontoblast-like cells in pulp tissue on days 3 and 7.
A comparative study of the E. faecalis antibiofilm efficacy of photoactivated curcumin, chlorophyll and riboflavin Riski Setyo Avianti; Sri Kunarti; Ari Subiyanto
Dental Journal (Majalah Kedokteran Gigi) Vol. 53 No. 2 (2020): June 2020
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v53.i2.p62-66

Abstract

Background: Photosensitisers play a vital role for reactive oxygen species (ROS) production in diode laser 405 nm therapy. Curcumin, chlorophyll and riboflavin have been used and viewed in several studies as effective photosensitisers for the elimination of Enterococcus faecalis (E. faecalis), a persistent microorganism that may cause endodontic failure. While each has given valuable and promising results as an alternative endodontic irrigant, no study has compared the efficacy of these three natural dyes. Purpose: To prove that the photosensitiser curcumin in diode laser 405 nm therapy is more effective for E. faecalis biofilm degradation than chlorophyll and riboflavin, and that a duration of irradiation of 90 seconds is more effective than 60 seconds. Methods: The biofilm monospecies E. faecalis was divided into two microplates with 96-well according to the irradiation periods: 60 seconds (Group 1) and 90 seconds (Group 2). Each group contained one control (without a photosensitiser) and three treatments were carried out by adding the photosensitisers curcumin, chlorophyll and riboflavin, where each treatment contained eleven samples. After irradiation for 60 seconds and 90 seconds, a crystal violet assay was carried out for each group. Results: The one-way ANOVA test showed a significant difference between groups based on the irradiation period. Tukey’s test showed each treatment in each group also showed a significant difference (p<0.05) with curcumin as the best substance to cause biofilm degradation in both groups. The duration of the irradiation showed that the longer the biofilm was illuminated, the lower the absorbance value or optical density (OD). Conclusion: Curcumin irradiated for 90 seconds gives better biofilm degradation on E. faecalis due to its natural properties and molecular structure, highlighting its efficacy in photodynamic therapy
Enamel remineralisation-inducing materials for caries prevention Sri Kunarti; Widya Saraswati; Dur Muhammad Lashari; Nadhifa Salma; Tasya Nafatila
Dental Journal (Majalah Kedokteran Gigi) Vol. 54 No. 3 (2021): September 2021
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v54.i3.p165-168

Abstract

Background: Dental caries is a multifactorial disease indicated by the progressive demineralisation process of dental tissue. It is caused by an imbalance between the remineralisation and demineralisation processes. The focus of caries management is on prevention. Providing materials that can induce remineralisation is one management of caries prevention. Various materials have been or are being researched, such as casein phosphopeptide amorphous calcium phosphate (CPP–ACP), tricalcium phosphate (fTCP), bioactive glass (BAG), and nanotechnologies such as nano-hydroxyapatite (n-HAP) and silver nano fluorine (NSF). Purpose: This study aims to review the development of enamel remineralisation inducing materials as a newer approach in caries prevention. Review: Various ingredients have been shown to increase enamel remineralisation through different mechanisms in preventing the development of carious lesions. Conclusion: CPP–ACP, fTCP, BAG, n-HAP, and NSF can induce enamel remineralisation as caries prevention agents. n-HAP and NSF are the most effective agents to enhance enamel remineralisation to prevent caries.