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An Analysis of Characteristics and Inhibition of Vaname Shrimp Chitosan on Streptococcus mutans I Gusti Agung Ayu Dharmawati; Nyoman Mastra; Ni Nyoman Dewi Supariani; I Wayan Juniarsana
Health Notions Vol 6, No 2 (2022): February
Publisher : Humanistic Network for Science and Technology (HNST)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33846/hn60205

Abstract

Indonesia is a maritime country with abundant marine products, but the processing of marine animal waste has not been a concern, the shell contains chitosan as a multifunctional biopolymer. Chitosan is a proven biomaterial that is non-toxic, biocompatible, and biodegradable compared to other polymers. The purpose of this study was to find out the content of Vaname shrimp chitosan and its inhibition against Streptococcus mutans bacteria. An experimental study was used, with a post test only control group design with the following treatments: control, 16 mg chitosan, 20 mg chitosan. Inhibitory power data (milli meter) was tested with One Way Anova. The research was carried out at the RAN Denpasar Laboratory, the Laboratory of the Faculty of Nutrition (Public Health, Airlangga University, Faculty of Pharmacy, Chemistry, Unair), UPT Analytical Laboratory Unud, and Microbiology Laboratory Unair. Results of this study indicated the content of carbohydrates, nitrogen, water, ash, protein, fat and the degree of deacetylation. Chitosan has an inhibitory effect on Streptococcus mutans bacteria. The quantity and quality of chitosan in Vaname shrimp, mets the requirements specified by Protan Biopolymer and was useful as an antimicrobial. Keywords: chitosan; vaname shrimp; characteristics; inhibition
Penyuluhan Kesehatan dan Pemeriksaan Kadar Gula Darah, Kolesterol Para Lansia di Wilayah Kerja UPT Kesmas Sukawati I. nyoman mastra
Jurnal Pengabmas Masyarakat Sehat (JPMS) Vol 2, No 1 (2020): Januari 2020
Publisher : Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33992/ms.v2i1.995

Abstract

Penyuluhan Kesehatan dan Pemeriksaan Kadar Gula Darah, Kolesterol  Para Lansia di Wilayah Kerja UPT Kesmas Sukawati I. I Wayan Merta, S.KM., M.Si k, Nyoman Mastra, S.KM., S.Pd., M.Si1, Ida Ayu Made Sri Arjani, S.IP., M.Erg2 Mertaiwayan9@gmail.com AbstractDiabetes mellitus (DM) and cholesterol disease are health problems not only in developed countries but also in developing countries Indonesia, where the preparation is quite high. This disease can attack all age groups, even elderly people are very at risk of developing this disease because their organs are not functioning optimally. Based on data from the Public Health Unit Sukawati I, there are 25 people with Diabetes Mellitus in Kemenuh Village. Community service activities carried out in Banjar Tengkulak Mas Kemenuh Village with a target of 40 people. The method in community service activities is lecture and examination of Blood Sugar and Cholesterol.The results obtained for a high level of knowledge before counseling 5 people (10%), after counseling increased to 34 people (75%) For examination of blood sugar levels with normal categories as many as 32 people (69%), above normal are as many as 14 people (31 %). For parameters of cholesterol with normal categories as many as 27 people (67%) and categories above normal as many as 14 people (33%). For the elderly whose blood sugar and cholesterol levels are normal to be maintained by maintaining the lifestyle behavior that has been carried out. As for the elderly whose blood sugar and cholesterol levels are above normal, which is changing diet by reducing the amount of rice, and increasing consumption of vegetables and fruits, regular exercise, and getting enough rest and controlling health regularly to the doctor. 
Penyuluhan dan Skrining Hepatitis B pada Ibu Hamil dengan Rapid Tes Di Kecamatan Kediri Kabupaten Tabanan Tahun 2018 I Gusti Ayu Sri Dhyanaputri; Cok Dewi Widya hana Sundari; I Nyoman Mastra; I Nyoman Jirna; I A.M. Sri Arjani; I Wayan Merta; I G. Sudarmanto; I G.A. Dewi sarihati
Jurnal Pengabmas Masyarakat Sehat (JPMS) Vol 1, No 2 (2019): April 2019
Publisher : Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33992/ms.v1i2.628

Abstract

Hepatitis B Virus can be transmitted by pregnant women to the fetus during labor. Therefore Hepatitis B examination during pregnancy is very necessary to find out whether during pregnancy pregnant women are infected with the Hepatitis B virus or not, so prevention can be done immediately. The purpose of this community service activity is to determine hepatitis B infection in pregnancy through HBsAg and Anti-HBs examination with a rapid test in Kediri District. The Community service activities is done by HBsAg and Anti HBs examination with Rapid Test, and provide counseling about Hepatitis B in pregnant women. Results of this community service showed from 100 pregnant women, obtained the age of  of pregnant women 26-30 years (32%), the highest gestational age are 7 months (21%), the highest number of gravidas was gravida 2 (47%). Knowledge about Hepatitis B after counseling is 92% of knowledge in good category. 6% of pregnant women were infected with the Hepatitis B virus.Keywords: Hepatitis B, Pregnant Woman, Hepatitis B Screening. 
UJI DAYA HAMBAT BERBAGAI KONSENTRASI PERASAN JERUK LEMON TERHADAP BAKTERI Propionibacterium acnes Kadek Eliana Kesuma Dewi; Nur Habibah; Nyoman Mastra
JST (Jurnal Sains dan Teknologi) Vol. 9 No. 1 (2020)
Publisher : Universitas Pendidikan Ganesha

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (121.28 KB) | DOI: 10.23887/jstundiksha.v9i1.19216

Abstract

Propionibacterium acnes merupakan salah satu bakteri yang menyebabkan terjadinya jerawat. Salah satu cara untuk mengatasi jerawat yaitu dengan memanfaatkan buah jeruk lemon. Beberapa penelitian terdahulu melaporkan bahwa jeruk lemon memiliki kandungan bahan aktif, antara lain karotenoid dan flavonoid  yang memiliki aktivitas antibakteri. Penelitian ini bertujuan untuk mengetahui kemampuan daya hambat berbagai konsentrasi perasan jeruk lemon terhadap pertumbuhan bakteri Propionibacterium acnes. Penelitian ini merupakan eksperimen murni dengan rancangan penelitian Posttest Only Control Design menggunakan metode difusi cakram Kirby Bauer dengan enam konsentrasi perasan buah jeruk lemon  yaitu 5, 12,5, 25, 50, 75, dan 100%. Kontrol kerja yang digunakan adalah kloramfenikol 30µg dan kontrol negatifnya adalah akuades steril. Hasil penelitian menunjukkan bahwa perasan buah jeruk lemon mampu menghambat pertumbuhan bakteri Propionibacterium acnes dengan rerata zona hambat berturut-turut yaitu sebesar 7,15; 9,20; 11,25; 12,50; 14,10 dan 16,90 mm dan termasuk ke dalam kategori zona hambat sedang dan kuat. Berdasarkan hasil penelitian yang diperoleh, dapat disimpulkan bahwa masing-masing konsentrasi perasan jeruk lemon memiliki kemampuan yang berbeda-beda terhadap pertumbuhan Propionibacterium acnes.
UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL DAUN JARAK PAGAR (Jatropha curcas Linn) PADA BERBAGAI KONSENTRASI TERHADAP PERTUMBUHAN BAKTERI Pseudomonas aeruginosa nyoman mastra
Meditory : The Journal of Medical Laboratory Vol 9, No 2 (2021): Volume 9, nomer 2, Desember 2021
Publisher : Jurusan Analisis Kesehatan, Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33992/m.v9i2.1736

Abstract

Background, Pseudomonas aeruginosa is the main bacterium in nosocomial infections. Pseudomonas aeruginosa is a bacterium that is resistant to almost all of antibiotics. As a result of these resistance cases, it is necessary to find the alternative products derived from natural ingredients antibacterial agents, one of which is Jatropha leaves. Objective, the objective of this study was to know the antibacterial activity of ethanol extract of Jatropha leaves at concentrations of 20, 40, 60 and 80% against the growth of Pseudomonas aeruginosa. Method, This study used experimental posttest only control group design with disc diffusion method used concentrations of 20, 40, 60 and 80%. Positive control using ciprofloxacin antibiotics and negative control using 96% ethanol. Results, of the study obtained the average diameter of inhibitory zones at concentrations of 20, 40, 60%, and 80% respectively were 11.5 mm, 18.3 mm, 19.5 mm, and 20.7 mm. Respectively the analysis of Least Significant Difference test, it was found that there were significant differences in inhibition zones in each extract concentration. Conclusion, the conclusion of this study is that the ethanol extract of Jatropha leaves has antibacterial activity against the bacterium Pseudomonas aeruginosa. Keywords: Jatropha leaves, extract, antibacterial activity, Pseudomonas aeruginosa
TINJAUAN TENTANG KOLESTEROL DARAH PADA SOPIR BUS PARIWISATA DI KABUPATEN BADUNG nyoman mastra
Meditory : The Journal of Medical Laboratory Vol 5, No 1 (2017): Juni 2017 Meditory
Publisher : Jurusan Analisis Kesehatan, Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (321.4 KB) | DOI: 10.33992/m.v5i1.102

Abstract

ABSTRACT Background The lifestyle of consuming high fatty foods, smoking and drinking coffee frequently in the work place could be expected to cause high cholesterol that affects the health of the tourism bus driver. Objective The aims study to determine a cholesterol levels in tourism bus driver at Badung regency. Methods This research is a descriptive study. Tourism bus driver who works at Indah Putra Transport. Result Of the 39 respondents obtained 18% had normal cholesterol levels and 82% had high cholesterol levels. Based on the frequency of smoking every day, the highest frequency indicated by smoking more than 20 cigarettes per day by 25 respondents (64%). The frequency of coffee consumption < 5 cups per day, 41% had high cholesterol levels, consume coffee > 5 cups of coffee per day, 41% have cholesterol levels high. Based on Physical activity habits 3˗4 times a week indicated 5% had high cholesterol levels and physical activity < 3 times a week indicated 77% had high cholesterol levels. 1˗5 year working period earned as much as 8% had high cholesterol levels, 6˗10 year working period earned 3% had normal cholesterol levels and 23% had high cholesterol and the rest of the respondents were 51% in working period above of 11 years have high cholesterol levels. Conclution It can be conceded that bad lifestyle of tourism bus drivers can affect the level of cholesterol. Keywords : driver, cholesterol, lifestyle
KADAR SERUM GLUTAMATE PIRUVAT TRANSAMINASE PECANDU MINUMAN KERAS DI BANJAR AMBENGAN DESA SAYAN UBUD GIANYAR nyoman mastra; I Gusti Ayu Tari Diva Pradnya Dewi; I Wayan Merta
Meditory : The Journal of Medical Laboratory Vol 4, No 2 (2016): Desember 2016
Publisher : Jurusan Analisis Kesehatan, Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (198.034 KB) | DOI: 10.33992/m.v4i2.44

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Background Banjar Ambengan is one banjar contained in Sayan village, where people still have the habit of gathering accompanied by excessive alcohol consumption. Consumption of alcohol has a direct relationship with the death cases caused by liver sirosis. The liver mechanism disturbance leads to the case of swelling as the result of the rise of serum glutamate piruvat transminase (SPGT) enzyme. Objective This study is aimed at investigating the SPGT level of the serum of the alcoholic. The study uses the non-probability sampling technique called snowball sampling. The samples of this study are thirty alcoholic men aging upper eighteen. The respondents will be interviewed to get the deeper information. The research reveals that 6 respondents have high SPGT level (20%) and 24 of them have normal SPGT level (80%). Result of the examination serum SGPT levels alcoholics in the study ranged from 11 to 79.20 U / L. The measurement results SGPT levels are high on the research respondents based on the characteristics of age at most 33.3%, based on the frequency of consumption of at most 33.3%, based on the period of consumption at most 50%, based on the volume of consumption at most 66.7%, based on the type of liquor consumed at most 66.7%, and based on the type of work at most 50%. Conclusion Keywords: Liquor, Liver, Addict, SGPT Levels
PERBEDAAN ZONA HAMBAT PERTUMBUHAN Staphylococcus aureus PADA BERBAGAI KONSENTRASI EKSTRAK ETANOL DAUN BIDURI SECARA IN VITRO nyoman mastra
Meditory : The Journal of Medical Laboratory Vol 6, No 1 (2018): Meditory, volume 6 , nomer 1 , Juni 2018
Publisher : Jurusan Analisis Kesehatan, Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (228.732 KB) | DOI: 10.33992/m.v6i1.227

Abstract

ABSTRACTBackground Staphylococcus aureus is a pathogenic bacteria that can cause infection. Biduri leaf has antibacterial compound such as tannin, flavonoid, saponin, and polyphenol.Objective This study aimed to know the difference of growth inhibition zone of Staphylococcus aureus at the various concentration of ethanol extract of biduri leaf and to determine an effective concentration.Methods This study was the true experiment with posttest only control design, used Kirby-Bauer disk diffusion method with five concentrations (20%, 40%, 60%, 80%,100%), positive control (chloramphenicol 30 µg) and negative control (ethanol 96%).Result The result of this study showed the average of inhibition zone diameter of each concentration consecutively 26,2 mm, 28,3 mm, 29,7 mm, 31 mm and 31,5 mm. One Way Anova statistic analysis showed that the value of p is 0.000 so there is the difference of growth inhibition zone of Staphylococcus aureus at various concentrations of ethanol extract of a biduri leaf.Conclusion The conclusion of this study is there are differences in growth inhibition zone of Staphylococcus aureus at various concentrations of ethanol extract of biduri leaf and an effective concentration that can inhibit of Staphylococcus aureus is concentration 20% with mean inhibition zone is 26,2 mm. Keywords: ethanol extract of biduri leaf; Staphylococcus aureus; inhibition zone
PERBEDAAN ZONA HAMBAT PERTUMBUHAN Staphylococcus aureus PADA BERBAGAI KONSENTRASI REBUSAN DAUN SALAM (Syzygium polyanthum) SECARA IN VITRO nyoman mastra
Meditory : The Journal of Medical Laboratory Vol 5, No 2 (2017): meditory ,Volume 5, No 2, Desember 2017
Publisher : Jurusan Analisis Kesehatan, Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (381.718 KB) | DOI: 10.33992/m.v5i2.138

Abstract

AbstractBackground Staphylococcus aureus is one of causes infection and this bacteria have been resistence for many antibiotic. Bay leaf have antibacterials substance, which stew leaves can be treat infection caused Staphylococcus aureus. The purpose of this study was to determine differences in growth inhibition zone of Staphylococcus aureus at various concentrations of water stew of bay leaf.Method The method of this study is true experiment with posstest only control design, and used Kirby Bauer disk diffusion method with various concentrations of water stewed of bay leaf (20%, 40%, 60%, 80%,100%), positive control (chloramfenicol 30 μg) and negative control (sterile distilled water). Result The result showed that the average diameter of inhibition zone in concentration 20%, 40%, 60%, 80%, and 100% is 7 mm, 8,4 mm, 9,6 mm, 10,5 mm and 11,5 mm. Based on statistical analysis using oneway ANOVA available the value of p (0,000) α (0,05), so the inhibition zone is significant difference of growth inhibition zone of Staphylococcus aureus at various concentrations of stewed water bay leaf. Conclusion Water stew of bay leaf can inhibit the growth of Staphylococcus aureus, and there are differences in growth inhibition zone of Staphylococcus aureus at various concentrations of water stew of bay leaf. Keywords: stew of bay leaf; Staphylococcus aureus; inhibition zone 
UJI AKTIVITAS ANTIBAKTERI REBUSAN DAUN SIRIH (Piper betle Linn) TERHADAP BAKTERI Streptococcus pyogenes nyoman mastra
Meditory : The Journal of Medical Laboratory Vol 7, No 1 (2019): Meditory, volume 7 , nomer 1 , Juni 2019
Publisher : Jurusan Analisis Kesehatan, Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (295.37 KB) | DOI: 10.33992/m.v7i1.639

Abstract

ABSTRACTBackground: Infection is a disease caused by pathogenic microbes and is very dynamic. ISPA is defined as an acute respiratory disease caused by infectious agents that were transmitted from human to human. One of the microbes that caused ISPA is Streptococcus pyogenes, which is a gram-positive bacteria and belongs to Streptococcus sp. β-hemolytic class A. Nowadays, many people are begin to use natural materials to deal with microbial infections. One of them is the betel leaf (Piper betle Linn.) Which has antibacterial and antibiotic components for the treatment of respiratory problems, and bacterial infectious diseases.Purpose: This research was conducted with the aim to know the antibacterial activity of betel leaves (Piper betle Linn) decoction against Streptococcus pyogenes bacteria. Methods: This research used true experimental design with Posttest Only Control Design, and data analysis with One Way Anova and Least Significant Deference (LSD). Betel leaves decoction water is made in concentrations of 20%, 40%, 60%, 80%, and 100%, then tested on Streptococcus pyogenes bacteria with two replicates and four repetitions.Result: From a total of 40 data obtained, the mean diameter of the inhibitory zone at each concentrations is 20% 0 mm, 40% 12.9 mm, 60% 22.7 mm, 80% 25.5 mm, and 100% 30 mm.Conclution: Betel leaves decoction water is able to inhibit the growth of Streptococcus pyogenes with significant difference of drag zone diameter at each concentration.  Keywords: Antibacterial, Betel Leaves, Streptococcus pyogenes