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Journal : Indonesian Journal of Chemistry

Decolorization and Transformation of Synthetic Dye Methylene Blue by Brown-Rot Fungus Fomitopsis pinicola Adi Setyo Purnomo; Hamdan Dwi Rizqi; Aulia Ulfi; Refdinal Nawfa; Herdayanto Sulistyo Putro
Indonesian Journal of Chemistry Vol 22, No 2 (2022)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijc.69834

Abstract

Methylene blue (MB) is a synthetic dye widely used in industries that is difficult to degrade in the environment due to its stability. Brown-rot fungus Fomitopsis pinicola is an organism that is known to be able to degrade some organic pollutants such as DDT, aldrin, dieldrin, and methyl orange dye. This study aimed to explore the ability of F. pinicola on MB biodecolorization, to identify metabolites and propose a biodecolorization pathway. F. pinicola was evaluated for MB biodecolorization on PDA agar and PDB liquid media. The metabolites were determined by using LCTOF/MS. The PDA agar medium's largest index decolorization (ID) was 0.915%. The MB decolorization in liquid PDB medium showed the highest percentage of decolorization of 92.56% at MB concentration of 100 mg/L after 14-days incubation. The analysis using LC-TOF/MS showed metabolites from MB biodecolorization, namely 3-amino-7-(methylamino) phenothiazin-5-ium (Azure C), 3-(dimethylamino)-7-(methylamino) phenothiazin-5-ium (Azure B), and 3,7-bis(dimethylamino)-4aH-phenothiazin-5-one. The MB degradation pathway was proposed through demethylation and oxidation reactions based on the detected product metabolites. These results indicated that F. pinicola is a suitable agent for biodecolorization of MB, and can potentially be used for bioremediation of MB waste in the environment.
Involvement of Fenton Reaction on Biodecolorization and Biodegradation of Methylene Blue Dye by Brown Rot Fungi Daedalea dickinsii Adi Setyo Purnomo; Alya Awinatul Rohmah; Weni Sri Ekowati; Hamdan Dwi Rizqi; Asranudin Asranudin
Indonesian Journal of Chemistry Vol 23, No 6 (2023)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijc.77689

Abstract

The disposal of dye wastewater has become a major global concern. Meanwhile, microorganisms have shown high potential in the treatment of wastewater pollutants. In this study, the involvement of the Fenton reaction in the biodecolorization and biodegradation of methylene blue (MB) by the brown rot fungus Daedalea dickinsii was investigated. Subsequently, D. dickinsii is a fungus capable of producing hydroxyl radicals (•OH). This experiment was conducted with an initial MB concentration of 75 mg/L, and different incubation times of 0, 7, 14, 21, and 28 d respectively. The result showed that the Fenton reaction played an important role, and this was demonstrated by the addition of FeSO4 as a Fe2+ source. The removal of MB by D. dickinsii with the addition of Fe2+ reached 91.454% at 28 d in a mineral salt medium. It was higher compared to D. dickinsii culture treatment without Fe2+ addition, 86.427%. Furthermore, the metabolic degradation product was analyzed using LC-TOF/MS and identified as 2-amino-3-hydroxy-5-(methylamino) benzenesulfonic acid and N-(3,4-dihydroxy phenyl)-N-methyl formamide.