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All Journal Media Penelitian dan Pengembangan Kesehatan Medical Journal of Indonesia Jurnal Teknologi Laboratorium
Budi Prasetyorini
Pusat Biomedis dan Teknologi Dasar Kesehatan, Jl. Percetakan Negara No. 29, Jakarta
Chemistry Decision Sciences, Operations Research & Management Medicine & Pharmacology Public Health Other

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CEK SILANG MIKROSKOPIS SEDIAAN DARAH MALARIA PADA MONITORING PENGOBATAN DIHIDROARTEMISININ-PIPERAKUIN DI KALIMANTAN DAN SULAWESI Ariyanti, Endah; E, Riyanti; Prasetyorini, Budi; Aisyah, Aisyah; Khairiri, Khairiri; Harun, Syahrial; Handayani, Sarwo; Tjitra, Emiliana
Media Penelitian dan Pengembangan Kesehatan Vol 22, No 4 Des (2012)
Publisher : Badan Penelitian dan Pengembangan Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | http://ejournal.litbang.depkes.go.id/index.php/MPK/article/view/2912

Abstract

Abstract Quality assurance of malaria microscopy is an important issue in health service and health research for a better case management. In monitoring Dyhydroartemisinin-Piperaquine, quality assurance was a part of this research activities at sentinel sites in Kalimantan and Sulawesi. This activity was carried out to confirmed diagnosis of malaria cases that could be analysed, and to evaluate the skill of microscopists to be improved in the future. Quality assurance was assessed based on the results of cross-checking malaria smears which done blindly by certified microscopist from Laboratory of  Parasitology ,National Institute of Health Research and Development, The quality of smears were mostly good, however the error rate was still high (10.9%). Therefore, a better and continuing planning and strategiy is needed to improve and mantain the quality  of  skill microscopists. Keywords: malaria; microscopic, Dyhydroartemisinin-Piperaquine   Abstrak Pemantapan kualitas mikroskopis malaria merupakan isue penting dalam pelayanan dan penelitian kesehatan untuk penanganan kasus yang lebih baik. Pada monitoring pengobatan Dihidroartemisinin-Piperakuin, pemantapan kualitas merupakan bagian dari kegiatan penelitian tersebut di lokasi sentinel (Kalimantan dan Sulawesi). Pemantapan dilakukan untuk mendapatkan kepastian diagnosis kasus malaria yang dapat dianalisis, dan sebagai evaluasi ketrampilan mikroskopis untuk perbaikan dan peningkatan di masa datang. Pemantapan kualitas dinilai berdasarkan hasil cek silang sediaan darah malaria secara blinded yang dilakukan oleh mikroskopis tersertifikasi dari Laboratorium Parasitologi Badan Litbang Kesehatan. Hasil cek silang menunjukkan kualitas  sediaan darah sebagian besar sudah baik meskipun untuk error rate masih tinggi mencapai 10,9%. Oleh sebab itu dibutuhkan rencana dan strategi yang baik dan berkelanjutan untuk memperbaiki dan mempertahankan kualitas tenaga mikroskopis malaria yang handal.   Keywords: malaria; mikroskopis; Dihidroartemisinin-Piperakuin
Efficacy of chloroquine, chloroquine plus sulphadoxine-pyrimethamine, and amodiaquine for treatment of vivax malaria in Bangka island, Indonesia: a randomized trial Tjitra, Emiliana; Maladi, Maladi; Prasetyorini, Budi; Suprianto, Sri; Harun, Syahrial; Nurhayati, Nurhayati; Yuwarni, Eni; Yandri, Boy; Laihad, Ferdinand; Ringwald, Pascal
Medical Journal of Indonesia Vol 17, No 2 (2008): April-June
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (571.518 KB) | DOI: 10.13181/mji.v17i2.307

Abstract

Plasmodium vivax malaria resistant to chloroquine is alarming in Indonesia and has been also reported in other countries. An alternative drug is needed. The study was a prospective evaluation and a comparative study of the therapeutic efficacy of chloroquine 25 mg base/kg bw for 3 days (CQ3, n=75), CQ3 plus sulfadoxine-pyrimethamine based on pyrimethamine dosage of 1.25 mg/kg bw single dose (SP1) [CQ3+SP1, n=84] and amodiaquine 25 mg base/kg bw for 3 days (AQ3, n=83) in symptomatic vivax malaria patients in children and adults. The new version of 2001 WHO test system was used in this study. PCR for genotyping was also done to validate and confirm the treatment outcomes. The therapeutic efficacy of CQ3, CQ3+SP1 and AQ3 on day 14 were very high (94.4%, 97.4% and 98.8%), and dropped on day 28 (81.7%, 87.2% and 96.2% by evaluable analysis; 78.9%. 82.0% and 92.5% after confirmation with PCR; and 74.7%, 78.0% and 90.2% by intention to treat analysis). Most of the ACPR cases (>96%) showed hematological recovery. Gametocyte carriages were documented on day 7 (2.9%, 1.3% and 1.2%), day 14 (4.3%, 1.3% and 1.2%) and day 28 (6.6%, 4.2% and none) in CQ3, CQ3+SP1 and AQ3 groups. Of these 3 regimens, AQ3 showed a better therapeutic efficacy than CQ3 and combined CQ3+SP1 by day 28. Introducing primaquine at the beginning of treatment day or giving a radical treatment in vivax malaria may improve the cure rate. (Med J Indones 2008; 17: 96-106)Keywords: P.vivax, chloroquine, sulfadoxine-pyrimethamine,amodiaquine, artesunate
Pengembangan Mikroskop Dengan Mikrokontroler dan Cahaya Monokromatik Untuk Mendeteksi Parasit Malaria Susanti, Ida; Handayani, Sarwo; Ekowatiningsih, Riyanti; Prasetyorini, Budi; A Yusnita, Endah; Ardianto, Donni Agus; K Widjaya, Sastra
Jurnal Teknologi Laboratorium Vol 6 No 2 (2017): 2017 (2)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (683.906 KB) | DOI: 10.29238/teknolabjournal.v6i2.59

Abstract

Malaria still become one of major health burden in Indonesia especially in remote areas of east Indonesia. Golden standard of malaria parasite detection is still microscopic technique using polychromatic light source whether from halogen or natural light source. A microscopic technique has a lot of benefits but still have weaknesses, such as time-consuming and bias on the reading by microscopist, because of artifact in the image. Aims of this study were to designed malaria parasites detection tool that is robust, fast, convenient and clear by minimizing artifact on the slide. Design of this study was laboratory experimental which modified simple microscope into an automatic microscope with table movement and webcam recording using a microcontroller and monochromatic light source. The wavelength of the light sources was 402nm(blue), 532 nm (green) and 650 nm (red), the intensity of each source differed. The reading of the slide image was conducted by two certified microscopists, who read 60 images of a thick and thin slide with three different live stage of Plasmodium falciparum live, which wearing, trophozoite and schizont. This study showed that modification of microscope was succeeded with automatic movement and webcam recording, process time in one step movement and recording approximately 10 seconds or 17minutes for 100 fields of view as confirmation process. The monochromatic light source has proven to give a clear and contrast field of view when the intensities were higher than 40 mW and the certified microscopist able to identified Plasmodium falciparum parasites. Data analysis of microscopist reading used nonparametric statistic Friedman by SPSS showed that correlation between images using monochromatic and polychromatic lights have meaningless differences in a thick and thin slide. However, hemozoin as a marker of Plasmodium falciparum parasite was less detected by monochromatic light used in this study.
Co-Authors Aisyah Aisyah Boy Yandri Donni Agus Ardianto, Donni Agus Emiliana Tjitra Endah A Yusnita, Endah Endah Ariyanti Eni Yuwarni Ferdinand Laihad Ida Susanti Khairiri Khairiri Maladi Maladi Nurhayati Nurhayati Pascal Ringwald Riyanti E Riyanti Ekowatiningsih, Riyanti Sarwo Handayani Sastra K Widjaya, Sastra Sri Suprianto Syahrial Harun