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All Journal Molecular and Cellular Biomedical Sciences (MCBS)
Rizal Rizal
Biomolecular and Biomedical Research Center, Aretha Medika Utama, Bandung
Biochemistry, Genetics & Molecular Biology Dentistry Immunology & microbiology Medicine & Pharmacology Neuroscience

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Chemical Constituents of Snake Fruit (Salacca zalacca (Gaert.) Voss) Peel and in silico Anti-aging Analysis Ermi Girsang; I Nyoman Ehrich Lister; Chrismis Novalinda Ginting; Adrian Khu; Butter Samin; Wahyu Widowati; Satrio Wibowo; Rizal Rizal
Molecular and Cellular Biomedical Sciences Vol 3, No 2 (2019)
Publisher : Cell and BioPharmaceutical Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1926.128 KB) | DOI: 10.21705/mcbs.v3i2.80

Abstract

Background: Skin aging is a condition where skin is unable to retain both its physiological and structural integrity. Plants is the main source of phtytochemicals compound with wide range of biological activities. Through the efforts of ongoing scientific researches, an increasing number of plant extracts and phytochemicals have been showed promising result as anti-aging agent. Snake fruit (Salacca zalacca (Gaert.) Voss) is tropical plant belongs to the palm tree family (Arecaceae) that served as important crop in Indonesia. Despite its utilization, the phytochemical compound available in snake fruit, especially its peel have not been well documented. Present study aimed to elucidate the phytochemical constituent of snake fruit peel and its anti-aging potency.Materials and Methods: Snake fruit peel extract (SPE) was subjected to qualitative phytochemical assay, high performance liquid chromatography, and molecular docking towards protein related in skin aging.Results: The screening showed SPE contained phytochemical compound belong to flavonoid, tannin, phenol, triterpenoid, saponin and alkaloid. Thus, based on the analysis only chlorogenic acid was present in SPE whilst rutin and caffeic acid were not detected. The SPE was contained chlorogenic acid around 1.074 mg/g dry weight. Chlorogenic acid had the high binding affinity towards matrix metalloproteinase (MMP)-1 (-9.4 kcal/mol).Conclusion: Current findings may provide scientific evidence for possible usage of SPE and its compounds as antioxidant and anti-aging agent.Keywords: Salacca zalacca, phytochemical compound, high performance liquid chromatography, anti-aging
Direct and Indirect Effect of TNFα and IFNγ Toward Apoptosis in Breast Cancer Cells Wahyu Widowati; Diana Krisanti Jasaputra; Sutiman Bambang Sumitro; Mochammad Aris Widodo; Ervi Afifah; Rizal Rizal; Dwi Davidson Rihibiha; Hanna Sari Widya Kusuma; Harry Murti; Indra Bachtiar; Ahmad Faried
Molecular and Cellular Biomedical Sciences Vol 2, No 2 (2018)
Publisher : Cell and BioPharmaceutical Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21705/mcbs.v2i2.21

Abstract

Background: Breast cancer (BC) is the leading cause of death cancer in women. Cancer therapies using TNFα and IFNγ have been recently developed by direct effects and activation of immune responses. This study was performed to evaluate the effects of TNFα and IFNγ directly, and TNFα and IFNγ secreted by Conditioned Medium-human Wharton’s Jelly Mesenchymal Stem Cells (CM-hWJMSCs) toward apoptosis of BC cells (MCF7).Materials and Methods: BC cells were induced by TNFα and IFNγ in 175 and 350ng/mL, respectively. CM-hWJMSCs were produced by co-culture hWJMSCs and NK cells that secreted TNFα, IFNγ, perforin (Prf1), granzyme B (GzmB) for treating BC cells. The BC cells were treated with CM-hWJMSCs in 50%. The expression of apoptotic genes Bax, p53, and the antiapoptotic gene Bcl-2 were determined using RT-PCR.Results: TNFα and IFNγ at concentration of 350 ng/mL induced higher Bax expression compared to 175 ng/mL. TNFα and IFNγ 350 ng/mL, 175 ng/mL induced p53 expression, whilst TNFα and IFNγ at 350 ng/mL decreased Bcl-2 expression. Perf1, GzmB, TNFα and IFNγ-containing CM-hWJMSCs induced significantly apoptosis percentage, induced Bax expression, but did not effect p53, Bcl-2 expression.Conclusion: TNFα and IFNγ directly induce Bax, p53, decrease Bcl-2 gene expression. The Prf1, GzmB, TNFα, IFNγ-containing CM-hWJMSCs induce apoptosis and Bax expression.Keywords: breast cancer, Wharton’s Jelly mesenchymal stem cells, TNFα, IFNγ
Isolation, Characterization, Proliferation and Differentiation of Synovial Membrane-derived Mesenchymal Stem Cells (SM-MSCs) from Osteoarthritis Patients Marlina Marlina; Rizki Rahmadian; Armenia Armenia; Wahyu Widowati; Rizal Rizal; Hanna Sari Widya Kusuma; Satrio Haryo Benowo Wibowo; Wahyu Setia Widodo; Ika Adhani Sholihah
Molecular and Cellular Biomedical Sciences Vol 4, No 2 (2020)
Publisher : Cell and BioPharmaceutical Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21705/mcbs.v4i2.100

Abstract

Background: Mesenchymal stem cells (MSCs) are the cells which have high renewal capacity and and are capable for differentiating into some types of cells. MSCs can be obtained from several tissues including bone marrow, synovial membrane, blood, adipose tissue and periosteum. The proliferation and self-repair ability of MSCs are the advantages to use as stem cells-based therapy of various diseases. The aim of this study was to determine the differentiation, characterization and proliferation of synovial membrane-derived MSCs (SM-MSCs).Materials and Methods: The cells proliferation capacity was determined by cell counting using trypan blue, characterization of MSCs (cluster of differentiation (CD)90, CD11b, CD73, CD34, CD19, CD45, CD105 and human leukocyte antigen-DR isotype (HLA-DR)) using flow cytometry analysis, and differentiation capability into three lineage cells was determined with red alcian blue, oil red O and alizarin staining.Results: The type culture of SM-MSCs was adherent and showed positive CD44, CD105, CD73, CD90 and negative of CD19, HLA-DR, CD11b, CD45, CD34 surface marker. Based on the result, SM-MSCs P3 showed differentiation potency into adipogenic, chondrogenic, and osteogenic lineage cells. The population doubling time of SM-MSCs has increased from P3 to P8. The population doubling time of SM-MSCs P3 was 1.69 days and SM-MSCs P8 was 3.64 days.Conclusion: The results indicated that SM-MCSCs from osteoarthritis patients are able to differentiate into osteocytes, chondrocytes, adipocytes and highly express of CD105, CD73, CD90, CD44 and negative for CD34, CD45, CD14, CD19.Keywords: synovial membrane, mesenchymal stromal cells, adipocyte, chondrocyte, osteocyte
Curcuma mangga Val. Extract as Antidiabetic Agent in 3T3-L1 Adipocyte Cells Dwiyati Pujimulyani; Wisnu Adi Yulianto; Astuti Setyawati; Rizal Rizal; Rismawati Laila Qodariah; Zakiyatul Khoiriyah; Annisa Arlisyah; Wahyu Widowati
Molecular and Cellular Biomedical Sciences Vol 4, No 1 (2020)
Publisher : Cell and BioPharmaceutical Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (8075.488 KB) | DOI: 10.21705/mcbs.v4i1.88

Abstract

Background: With the increase of diabetes mellitus (DM) prevalence, natural product emerged as complementary source on the development of new drug for this disease. White saffron (Curcuma mangga Val.) is a widely available plant found in Indonesia which often used traditionally as medicine for various ailment. Unfortunately scientific evidence of its antidiabetic activity has not been described very well. Present study was trying to evaluate the antidiabetic potential of white saffron based on the change of lipid accumulation.Materials and Methods: Cells viability assay was done using 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) reagent to determine the safe concentrations of C. mangga Val. extract and its fractions including hexane, ethyl acetate, butanol, ethanol, water fractions and curcumol for the further assay. The preadipocyte cells (3T3-L1) were grown and differentiated into adipocyte cells using 3-isobutyl-1-methylxanthine (IBMX), dexamethasone and insulin. The adipocyte cells were treated with C. mangga Val. extract (CME) (the safest fraction at all concentrations) for 24 h. Oil red O staining was used to measure the lipid accumulation in adipocyte cells.Results: The CME was not toxic and able to decrease the lipid droplets of the 3T3-L1 adipocyte cells.Conclusion: The CME has potential antidiabetic activity due to ability to decrease the lipid droplet without disturbing the viability of the 3T3-L1 adipocyte cells.Keywords: white saffron, Curcuma mangga Val., antidiabetic
Co-Authors Adrian Adrian Ahmad Faried Annisa Arlisyah Armenia, Armenia Astuti Setyawati Butter Samin Chrismis Novalinda Ginting Diana Krisanti Jasaputra Dwi Davidson Rihibiha Dwiyati Pujimulyani Ermi Girsang Ervi Afifah Hanna Sari Widya Kusuma Harry Murti I Nyoman Ehrich Lister Ika Adhani Sholihah Indra Bachtiar Marlina . Mochammad Aris Widodo Rismawati Laila Qodariah Rizki Rahmadian Satrio Haryo Benowo Wibowo Satrio Wibowo Sutiman Bambang Sumitro Wahyu Setia Widodo Wahyu Widowati Wisnu Adi Yulianto Zakiyatul Khoiriyah