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All Journal Jurnal Kesehatan Prima Avicenna : Journal of Health Research
Rohmi Rohmi
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Health Professions Nursing Public Health

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Journal : Jurnal Kesehatan Prima

 1 
DRAGON FRUIT (Hylocereus polyrhizus) AS NATURAL DYES STAINING FOR BACTERIA Yunan Jiwintarum; Rohmi Rohmi; I Dewa Putu Martha Prayuda
Jurnal Kesehatan Prima Vol 10, No 2 (2016): Jurnal Kesehatan Prima
Publisher : poltekkes kemenkes mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (0.272 KB) | DOI: 10.32807/jkp.v10i2.24

Abstract

Abstract :  the bacteria is difficult to be perceived with a light microscope because it can not adsorb or refract the light, consequently the dye applies for staining the bacteria or their background. Synthetic dye has a fairly expensive price than natural dye. This study aims to determine the usage of dragon fruit (Hylocereus polyrhizus) as a natural dye for staining the bacteria. This research is quasi experimental, while the data will be analysed by descriptive analysis with 3 treatments, in each uses eight samples. The study showed that on the quality of stains, bacterium Staphylococcus aureus and E. coli by using the juice of the dragon is obtained a result, as many as 4 slides preparations of bacteria Staphylococcus aureus (33.33%) and 4 slides preparations of bacteria E .coli (33.33%) were not stained. Therefore, the result on the research can be concluded that the dragon fruit (Hylocereus polyrhizus) can not be used as a natural dye for staining bacteria.
A CHARACTERISTIC OF MORPHOLOGY, COLONY AND BIOCHEMISTRY OF BACTERIA THAT ISOLATED FROM SEDIMENTS OF MOSQUITO BREEDING LAGOON Maruni wiwin Diarti; Rohmi rohmi; Yuri Syaffitri Kinanti Achmad; Yunan Jiwintarum
Jurnal Kesehatan Prima Vol 11, No 2 (2017): Jurnal Kesehatan Prima
Publisher : poltekkes kemenkes mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (0.272 KB) | DOI: 10.32807/jkp.v11i2.6

Abstract

The lagoon is a form of semi-enclosed bay which is one type of an estuary. In lagoon water there is a meeting and mixing of freshwater and seawater thus it causes free relationship between the sea and the source of freshwater. The effect of tides that mixed with freshwater will cause a change of the aquatic character instantly. It will certainly affect the diversity of organism species, including bacteria. Some types of bacteria, one of them is   Bacillus, it is basically soil bacteria, but commonly found in fresh and brackish waters. The purpose of this study was to determine the characteristics of morphology, colonies and biochemistry of bacteria that isolated from sediments of mosquito breeding lagoon in the coast of Medana village, Tanjung district, North Lombok regency. This research was descriptive observational, used non random purposive sampling with 15 samples. Data collected in the form of types of bacteria found in 15 points from sediments of mosquito breeding lagoon. The data analysis used descriptive analysis. The results of 15 points of lagoon sediments that isolated, from 15 bacteria colonies that was identified as morphological, colonies and biochemical characteristics found three types of bacteria: Aeramonas schubertii, Bacillus subtilis, and Enterbacter agglomerans. The conclusion is the sediments of mosquito breeding lagoon in the coast of Medana Village, Tanjung district, North Lombok regency is obtained the bacteria, for instace: Aeramonas schubertii, Bacillus subtilis, and Enterobacter agglomerans.
Ubi Jalar Putih (Ipomoea Batatas L.) Media Alternatif Pertumbuhan Aspergillus Niger Rohmi Rohmi; Zainal Fikri; Ni Ketut Riska Pujasari
Jurnal Kesehatan Prima Vol 13, No 2 (2019): AGUSTUS
Publisher : poltekkes kemenkes mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (0.272 KB) | DOI: 10.32807/jkp.v13i2.234

Abstract

Jamur Aspergilus niger menghasilkan alergan yang menyebabkan reaksi alergi, yaitu dapat menyebabkan reaksi hipersensitivitas seperti asma dan alveolitis  pada manusia. Ubi jalar putih (Ipomoea batatas L.) mengandung karbohidrat yang dapat digunakan sebagai pengganti sumber karbohidrat pada media PDA. Tujuan penelitian ini adalah untuk mengetahui pengaruh Penggunaan ubi jalar putih (Ipomoea batatas L.) sebagai media alternatif pertumbuhan jamur Aspergillus niger. Penelitian ini bersifat true eksperimet dengan menggunakan 6 replikasi dan 4 perlakuan yaitu media PDA sebagai kontrol, media tepung ubi jalar putih dengan konsentrasi 10%, 20%, dan 30%. Hasil uji laboratorium pada media PDA pertumbuhan diameter jamur Aspergilus niger adalah 43.5 mm dengan sporulasi lebat dan miselium tebal, pada media alternatif tepung ubi jalar putih (Ipomoea batatas L.) pada konsentrasi 10% memiliki pertumbuhan diameter jamur Aspergilus niger adalah 40.8 mm dengan sporulasi tipis dan miselium putih tipis, pada media alternatif tepung ubi jalar putih (Ipomoea batatas L.) pada konsentrasi 20% memiliki pertumbuhan diameter jamur Aspergilus niger adalah 57 mm dengan sporulasi cukup lebat dan miselium putih tipis, dan pada media alternatif tepung ubi jalar putih (Ipomoea batatas L.) pada konsentrasi 30% memiliki pertumbuhan diameter jamur Aspergilus niger adalah 37.5 mm dengan sporulasi cukup lebat dan miselium putih tipis. Dapat disimpulkan bahwa tepung ubi jalar putih (Ipomoea batatas L.) dapat digunakan sebagai media alternatif untuk pertumbuhan Aspergillus niger.
Co-Authors Ady Irawan Ahmad Rois Syujak Ahmad Syauqi Mubarok Ahmad Syauqi Mubarok Ahmad Zamani Alga Auraldi Andriani Noerlita Ningrum Anggie Pradana Putri Anggie Pradana Putri Ani Nur Fauziah Anisa Hidayati Anita Dewi Lieskusumastuti Aquartuti Tri Darmayanti Aquartuti Tri Darmayanti Budi Setiawan Manurung Catur Setyorini Catur Setyorini Daryanto Daryanto Dewi Arradini Dwi Joko Yulianto Endri Adi Saputra Etik Sulistyorini Eva Leny Alviani Febria Reza Kurnia Galuh Sindi Wardani Galuh Sindi Wardhani Handayani, Rina Tri Heni Ernawati Heni Heni Ernawati I Dewa Putu Martha Prayuda Indarto - Irfan Armanto Isnani Nurhayati Isnani Nurhayati Joko Prayitno Joko Tri Atmojo Karmadi Karmadi Kiki Puspitasary Kiki Puspitasary Lilik Hanifah Lilik Hanifah Maruni Wiwin Diarti Meliana Novitasari Mifta Aditya Muhamad Yuli Kartiko Nabhani Nabhani Nasrul Sani Ndaru Syukma Putra Ni Ketut Riska Pujasari Nova Rahma Widyaningrum Nurrochim Nurrochim Puji Nur Rokhmatun Ratna Wirawati Rejo Rejo Rejo Rejo Rinandita Febri Susanti Rinandita Febri Susanti Sabatun Sabatun Saras Kuntari Saras Kuntari SATRIYAS ILYAS Selvia Anggitasari Siti Maesaroh Sri Hartutik Sri Iswahyuni Sri Iswahyuni SRI LESTARI Sri Sayekti Heni Sunaryanti Sri Sayekti Heni Sunaryanti Sri Suparti Suminanto Suminanto Tatik Trisnowati Tio Guntur Prabowo Tri Yuniarti Ulin Nuha Widiyanto, Aris Yesi Ihdina Fityatal Hasanah Yunan Jiwintarum Yuri Syaffitri Kinanti Achmad Zainal Fikri