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EFEK TOKSISITAS FRAKSI ETIL ASETAT AKAR SENGGANI (Melastoma affine D.Don ) PADA ORGAN HEPAR, GINJAL DAN TESTIS Saiful Bachri, Moch.
PHARMACIANA Vol 1, No 1: Mei 2011
Publisher : PHARMACIANA

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Senggani (Melastoma affine D.Don) merupakan tanaman yang banyak ditemukan di wilayah Asia tenggara dan China. Tanaman ini banyak di gunakan sebagai obat tradisional. Penelitian ini bertujuan untuk mengetahui efek toksik senggani terhadap organ-organ vital. Sejumlah 32 ekor tikus jantan jenis Wistar di bagi menjadi 5 kelompok. Kelompok Kontrol (n=8 ), kelompok Fraksi Etil asetat akar senggani (FEAS) dosis 62.5 mg/kgBB (n=6 ), dosis 125 mg/kgBB (n=6), dosis 250 mg/kgBB (n=6), dosis 500 mg/kgBB (n=6). Kelompok kontrol disuntik secara per oral dengan bahan pensuspensi FEAS yaitu CMC Na 0.5 %. Pemberian fraksi etil asetat akar senggani selama 22 hari. Pada hari ke-23 dilakukan pembedahan dan dilakukan pemeriksaan histopatologik pada organ testis, ginjal dan hepar. Hasil pengamatan pada ginjal dan hepar, pemberian FEAS untuk semua dosis tidak ada perbedaan dibandingkan dengan kelompok kontrol, sementara pada organ testis pemberian FEAS dosis 250 mg/kgBB dan 500 mg/kgBB menunjukkan terjadinya nekrosis pada tubulus seminiferus dan terlihat jumlah sperma mengalami penurunan. Kesimpulan penelitian ini, pemberian fraksi etil asetat akar senggani menyebabkan efek toksik pada organ testis ditunjukkan dengan adanya nekrosis pada tubulus seminiferus.

EFEK HEPATOPROTEKTIF EKSTRAK METANOL JAHE MERAH (Zingiber officinale Roscoe) PADA MENCIT JANTAN YANG DIINDUKSI CCl4 Saiful Bachri, Moch.
PHARMACIANA Vol 1, No 2: November 2011
Publisher : PHARMACIANA

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Ekstrak MeOH jahe merah (Zingiber officinale Roscoe) telah diteliti efek hepatoprotektif pada dosis 100 mg/kgBB dan 200 mg/kgBB dengan menggunakan metode induksi CCl4. Mencit jantan galur Swiss (25±1,5 g) diberi perlakuan dengan jahe merah (100 mg/kgBB dan 200 mg/kgBB) dan Sillymarin (100 mg/kgBB) selama dua minggu sebelum diinduksi CCl4, 0,5 ml/kgBB secara intraperitonial. Setelah 24 jam mencit dibunuh dan diambil darah melalui vena porta untuk diuji aktivitas SGPT dan SGOT. Hasil penelitian menunjukkan bahwa aktivitas SGPT dari ekstrak MeOH jahe merah dengan dosis 100 mg/kgBB dan 200 mg/kgBB, Sillymarin, kontrol, dan normal berturut turut, (34,51±5,3427) (38±4,28) (27,26±1,06) (36,61±1,87) dan 25,37±2,01 IU/L. Sedangkan aktivitas SGOT dari Ekstrak MeOH jahe merah dengan dosis 100 mg/kgBB dan 200 mg/kgBB, Sillymarin, kontrol dan kelompok Normal berturut turut, (88,88±0,94) (83,88±3,91) (83,99±7,24) (92,97±8,16) (82,99±3,97)U/L. Ekstrak MeOH jahe merah dosis 200 mg/kgBB dan Sillymarin secara signifikan (p < 0,05) dapat menurunkan aktivitas SGPT dan SGOT. Dari hasil penelitian menunjukkan bahwa ekstrak MeOH jahe merah mempunyai efek hepatoprotektif.

The gastroprotective effects of canna edulis ker. tuber starch on peptic ulcer and the histopathological profile of rat stomach Najini, Robby; Bachri, Moch Saiful; Yuliani, Sapto
Pharmaciana Vol 8, No 1 (2018): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Ganyong rhizome (Canna edulis Ker.) empirically use for treating gastritis (peptic ulcers). Ganyong rhizome contains carbohydrates and flavonoid that can reduce inflammation in the gastric tissues and againsts free radicals that are contributing to the pathogenesis of peptic ulcers. This research aims to know the gastroprotective effect of ganyong rhizome on white female rats, Wistar strain, which were given alcohol (ethanol 96%) by dose 1 ml/200gBB and measuring the total flavonoids content in ganyong rhizome. This research is Post Test Only Control Group Design, using 30 rats, weight 150-200 g, age 6-8 weeks, health condition. It is divided into six groups. Group I (normal) were fed and watered only, Group II (control) were given a CMC-Na 0,5%, Group III-V were given a suspension of ganyong rhizome starch (SGRS) with each doses 250, 500, and 1000 mg/kgBB, then Group VI were given a sucralfate dose 360 mg/kgBB. Rats were given oral treatment for 14 days. An hour after the treatment on 14th day, the whole groups unless the group I were given ethanol 96% 1 ml/200gBB orally. Twenty four hours later the rats were sacrificed, dissected and taken its gastric for further analyzed the number of ulcers as well as the description of histopathology of the gastric. The results showed that ganyong rhizome starch can decrease the index ulcer and increase the protection ratio value to the control group with results of doses SGRS 250, 500, 1000 mg/kgBB sequentially i.e., 12,5; 50; and 75%. The description of histopathology also showed significant improvement of gastric tissue at a dose of 1000 mg/kgBB. In addition, on TLC-Densitometri test also obtained the total flavonoid compound in ganyong rhizome is 112,49 Â± 7,97 ppm. Conclusions of this research are that the ganyong rhizome positive contains flavonoid and it has gastroprotective effect by decreasing the number of index ulcer, increasing the protection ratio value, and improving the histopathology of gastric tissue which had given alcohol.

EFEK HEPATOPROTEKTIF SERBUK AKAR PASAK BUMI (Eurycoma longifolia Jack.) DILIHAT DARI AKTIVITAS SGPTSGOT TIKUS JANTAN YANG DIINDUKSI CCl4 Adikusuma, Wirawan; Bachri, Moch. Saiful
Pharmaciana Vol 4, No 2 (2014): Pharmaciana
Publisher : Universitas Ahmad Dahlan

This study was designed to evaluate the hepatoprotective effect of the powder Eurycomalongifolia Jack. From the activity level of SGPT-SGOT on CCl4-induced in male rats. Twentyfive male rats (150-250 g) divide in to 5 groups. Group I treated with aquadest was kept asnormal, group II treated with a single dose of CCl4 (1 ml/ kg BW i.p), group III and IV weretreated with Eurycoma longifolia Jack. (100 mg/kg BW and 200 mg/kg BW p.o) respectivelyand CCl4 (1 ml/kg BW i.p), group V treated with a single dose of curcumin (100 mg/kg BWp.o) and CCl4 (1 ml/kg BW i.p). Blood was collected from vena porta for determination ofSGPT-SGOT. The study showed the activity level of SGPT from the rats was treated byEurycoma longifolia Jack. 100 mg/kg BW and 200 mg/kg BW, Curcumin, and control groupsare 150.0Â±5.099 U/L; 113.6Â±5.508 U/L; 60.5Â±2.887 U/L; and 129.0Â±6.055 U/L respectively. Mean while the activity level of SGOT from the rats was treated by Eurycoma longifolia Jack.100 mg/ kg BW and 200 mg/ kg BW, Curcumin, and control groups are 369.4Â±11.165;263.0Â±1.803; 194.5Â±7.448; and 451.5Â±16.759 U/L respectively. The Eurycoma longifoliaJack. powder and Curcumin significantly (p < 0.05) declines two enzymes (SGPT and SGOT)than control group. The results concluded that Eurycoma longifolia Jack. powder hashepatoprotective effect.

EFEK HEPATOPROTEKTIF EKSTRAK METANOL JAHE MERAH (Zingiber officinale Roscoe) PADA MENCIT JANTAN YANG DIINDUKSI CCl4 Bachri, Moch Saiful
Pharmaciana Vol 1, No 2 (2011): Pharmaciana
Publisher : Universitas Ahmad Dahlan

MeOH extract of Zingiber officinale Roscoe (ZO) were investigated forhepatoprotective effect at the doses (p.o) of 100 and 200 mg/kg body weight using CCl4induction method. Male Swiss mouse (25Â±1,5 g) were treated with ZO doses 100 and200 mg/kg bw and Sillymarin (100 mg/kg bw) for two week before treatment with asingle dose of CCl4, 0.5 ml/kg bw, intraperitoneally. The mouse were sacrifactivity ofSGPT-SGOT. The study showed the activity of SGPT of MeOH extract of ZO doses 100mg/kg bw and 200 mg/kg bw, Sillymarin, control and normal groups are (34,51Â±5,34)(27,38Â±4,28) (27,26Â±1,06) (36,61Â±1,87) dan (25,37Â±2,01I) U/L. respectively. Whilethe activity of SGOT of MeOH extract of ZO doses 100 mg/kg bw and 200 mg/kg bw,Sillymarin, control and normal groups are (88,88Â±0,94) (83,88Â±3,91) (83,99Â±7,24)(92,97Â±8,16) (82,99Â±3,97) U/L.respectively. MeOH extract of ZO dose 200 mg/kg BWand Sillymarinsignificantly (p < 0,05) decrease the activities two enzymes (SGPT andSGOT). The results showed MeOH extract Zingiber officinale Roscoe hashepatoprotective effect.

EFEK TOKSISITAS FRAKSI ETIL ASETAT AKAR SENGGANI (Melastoma affine D.Don ) PADA ORGAN HEPAR, GINJAL DAN TESTIS Bachri, Moch Saiful
Pharmaciana Vol 1, No 1 (2011): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Melastoma affine (MA) is a medicinal plant commonly found in south East Asiaand China. The aim of the study is to investigate the toxicity effect of MA toward vitalorgans. Thirty two Wistar adult male rats were divided into five groups consisting of sixanimals each the rats in group except control group, eight animals. The rats in group I(control) were administered 0.5% CMC Na orally, while CMC Na suspension of ethylacetate fraction of MA root (62.5, 125, 250,and 500 mg/kg body weight) were given tothe II, III, IV and V groups, respectively. The CMC Na suspensions of ethyl acetatefraction of MA root were administered for 22 days. Rats were sacrificed at the 23th day.Liver, kidney, testis were collected for histopathological observation. The observationresult on liver and kidney showed no difference compare to control groups, meanwhileon testis, the administration of ethyl acetate fraction of MA root at dose (250, 500 mg/kgbody weight) caused necrosis on seminiferus tubules and decreasing sperm counting.The conclusion, administration of ethyl acetate fraction of MA root caused toxicityeffect on testis with necrosis on seminiferus tubules.

Potensi ekstrak etanol 70% akar saluang balum (Lavanga sarmentosa blume kurz) terhadap kualitas dan viabilitas sperma mencit Syarif, Yaumi Musfirah; Bachri, Moch Saiful; Nurani, Laela Hayu
Pharmaciana Vol 6, No 2 (2016): Pharmaciana
Publisher : Universitas Ahmad Dahlan

The present study tried to investigate potency of L. sarmentosa root extract on quality and viability of mice sperm.This study used 25 Swiss male mice were randomly divided into five experimental groups of 5. The ethanolic extract of L. Sarmentosa (100, 200, 400 mg/kg b.w/days), control and X-gra were administered orally for 14 days. The animals were evaluated for sperma quality (sperm count and sperm motility) and viability at 14 days treatment. The results showed that administration of L.sarmentosa roots extract significantly increasing the sperm count (970x106cell/mL) at 400 mg/kg b.w/days (P 0.0040). The extract dose of 200 mg/Kg b.w and 400 mg/Kg b.w had the highest motility values (95%). Viability of sperm are 83.8%; 96.0%; 87.8% respectively at a dose of 100 mg/kg b.w, 200 mg/kg b.w and 400 mg/kg b.w. Present finding oral administration ofÂ L. sarmentosa roots extract has a potency of the quality and viability of mice sperm.

PENGARUH PEMBERIAN EKSTRAK ETANOL DAN KLOROFORM DAUN SIRSAK TERHADAP KOLESTEROL TOTAL DAN TRIGLISERIDA PADA TIKUS YANG DIINDUKSI ALOKSAN Firmansyah, Deni; Bachri, Moch Saiful; Nurkhasanah, Nurkhasanah
Pharmaciana Vol 6, No 1 (2016): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Annona muricata plant is a medicinal plant using by research and drug for human healthy including diabetes mellitus. This research aimed to determine the effect of ethanol extract and chloroform extract of the sirsak leaf as antidiabetes mellitus. This research was conducted in 9 groups of male Wistar rats consisting of 5 rats per group, consisting of normal control, alloxan control, glibenclamide control dose of 10 mg/Kg BW, group of ethanol extract sirsak leaf dose of 50 mg/Kg BW; 100 mg/Kg BW; 200 mg/Kg BW, and group of chloroform extract sirsak leaf dose of 50 mg/kg BW; 100 mg/Kg BW; 250 mg/Kg BW. Tests carried out for 2 weeks. It also conducted assays of total flavonoids and histopathological tests of pancreatic Î² cells. Results of this research showed that the ethanol extract ofÂ sirsak leaves dose of 200 mg/Kg BW has activity in decreasing blood glucose levels better than any other group. Results of the ethanol extract and chloroform extract all doses except the ethanol extract treatment group dose of 200 mg/kg BW and 100 mg/kg BW after a 14-day look is still high triglyceride levels and decrease in total cholesterol (TC) were seen in all group Ethanol extract dose of 200 mg / kgBWÂ has a good potential to reduce levels of triglycerides and total cholesterol. The conclusion of this research is the ethanol extract of sirsak leaf have activity antidiabetic mellitusand decreased total cholesterol.Â

Efek Pemberian Subkronis Ekstrak Etanol Rimpang Kunyit (Curcuma Longa Linn.) Pada Hati Tikus Maharani, Hestuning Winda; Bachri, Moch. Saiful
Media Farmasi: Jurnal Ilmu Farmasi Vol 12, No 2: September 2015
Publisher : Universitas Ahmad Dahlan

Penelitian ini bertujuan untuk mengetahui efek toksik ekstrak etanol rimpang kunyit (Curcuma longa Linn.) terhadap hati tikus jantan galur Wistar jika diberikan jangka panjang (subkronis) dengan melihat aktivitas AST dan ALT. Tikus sejumlah 32 ekor, dibagi menjadi empat kelompok. Kelompok I (kontrol) diberi larutan CMC-Na 0,5%, kelompok II, III dan IV diberi ekstrak etanol rimpang kunyit dosis 50, 100 dan 200 mg/kgBB. Perlakuan selama 31 hari, kemudian data hasil uji dianalisis menggunakan Uji Kruskal-Wallis dilanjutkan Uji Mann-Whitney U dengan taraf kepercayaan 95%. Dari hasil penelititan diketahui bahwa aktivitas AST dosis 50, 100 dan 200 mg/kgBB berturut-turut adalah 18,75±0,03 U/L, 18,75±0,03 U/L, dan 20,39±0,05 U/L. Evektivitas ALT adalah 23,42±0,02 U/L, 23,67±0,03 U/L, dan 24,45±0,05 U/L. Kontrol AST adalah sebesar 18,69±0,03 U/L dan ALT sebesar 23,49±0,02 U/L. Hasil analisis menunjukkan nilai AST dosis 50 dan 100 mg/kgBB tidak berbeda signifikan dengan AST kontrol, tetapi nilai AST dosis 200 mg/kgBB berbeda signifikan dengan kontrol namun masih dalam range normal, nilai ALT ketiga dosis tidak berbeda signifikan dengan ALT kontrol. Kesimpulannya adalah pemberian ekstrak etanol kunyit dosis 50, 100 dan 200 mg/kgBB selama 31 hari tidak menyebabkan toksik pada hati tikus jantan galur Wistar dilihat dari nilai AST dan ALT yang masih dalam range normal. Kata Kunci : kunyit (Curcuma longa Linn.), toksisitas subkronis, ALT, AST

PENGARUH EKSTRAK AKAR SENGGANI (Melastoma affine D.Don) TERHADAP MOTILITAS DAN JUMLAH SPERMA TIKUS Bachri, Moch. Saiful; Donatus, Imono Argo; Wahyuono, Subagus
JFIOnline | Print ISSN 1412-1107 | e-ISSN 2355-696X Vol 4, No 2 (2008)
Publisher : Indonesian Research Gateway

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Spermatogenesis effect of ethanol extract senggani root (Melastoma affine D. Don) was proved. Furthermore, the extract was fractionated with ethyl acetate and was investigated toward spermatogenesis effect to explore natural product as male contraception. The subject of this research were five groups of thirty two fertile male Wistar rat,. Four groups were treated with ethyl acetate fraction of senggani root in gradually 500; 250; 125; and 62,5 mg/kg body weight dose. The fifth group as negative control was treated by CMC Na 0,5 %. Every groups was treated by orally administration for twenty two days period of time, once a day. In twenty third day, the rats were killed then observed toward the motility, sperm count, and testis histopathology. Results of the research indicated that there were influence toward spermatogenesis effect from ethyl acetate fraction of senggani root, especially at sperm motility and sperm count. Decrease of sperm motility after treatment was compared with negative control group. The percentage of the sperm count decreased at 500; 250 mg/kg body weight dose from ethyl acetate fraction was 49,74 % and 48,21.This decreased probably were caused by seminiferous tubules necrotic. ABSTRAK Pemberian ekstrak etanol akar senggani (Melastoma affine D. Don) telah terbukti dapat menyebabkan berkurangnya jumlah dan motilitas sperma tikus. Berdasarkan hasil penelitian tersebut dilakukan fraksinasi ekstrak etanol akar senggani dengan etil asetat kemudian fraksi itu di uji cobakan pada tikus dengan tujuan untuk eksplorasi bahan alam yang dapat dipakai sebagai bahan kontrasepsi pria. Penelitian ini menggunakan 32 ekor tikus jantan fertil galur Wistar, umur 2,5-4 bulan dengan bobot 145-350 g dan dibagi menjadi 5 kelompok. Empat kelompok merupakan kelompok perlakuan fraksi etil asetat akar senggani dengan dosis 500; 250; 125; dan 62,5 mg/kgBB. Kelompok V merupakan kelompok kontrol negatif dengan perlakuan CMC Na 0,5 %. Semua kelompok diberi perlakuan secara per oral selama 22 hari dengan frekuensi pemberian sekali sehari. Pada hari ke-23 tikus dikorbankan dan diamati motilitas, dan jumlah atau angka sperma. Dilakukan juga pengamatan histopatologi organ testis. Hasil penelitian menunjukkan ada pengaruh pemberian fraksi etil asetat akar senggani terhadap motilitas dan jumlah (angka) sperma. Motilitas sperma menjadi berkurang pada semua dosis perlakuan jika dibandingkan dengan kelompok kontrol. Penurunan motilitas sperma terbesar pada dosis 250 mg/kgBB. Jumlah sperma juga mengalami penurunan untuk semua dosis perlakuan. Persentase penurunan jumlah sperma terbesar pada dosis 500 ; 250 mg/kgBB sebesar 49,74 % , dan 48,21 %. Penurunan ini kemungkinan diakibatkan oleh rusaknya tubulus seminiferus testis yang mengalami nekrosis

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