Pudji Srianto
Department Of Veterinary Reproduction, Faculty Of Veterinary Medicine, Universitas Airlangga

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Pengukuran Kadar Hormon Progesteron dan Deteksi Birahi pada Sapi Perah yang Disinkronisasi dengan CIDR (Controlled Internal Drug Release) Oky Setyo Widodo; Pudji Srianto; Shelly Wulandari
Jurnal Medik Veteriner Vol. 2 No. 2 (2019): October
Publisher : Faculty of Veterinary Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (428.556 KB) | DOI: 10.20473/jmv.vol2.iss2.2019.133-139

Abstract

Deteksi birahi dan program perkawinan merupakan cara yang tepat untuk dapat mengoptimalkan performa reproduksi pada sapi perah. Rancangan penelitian yang dilakukan dalam penelitian ini adalah pretest-postest design. Terdiri dari empat macam perlakuan yaitu P1C1, P1C2, P2C1, dan P2C2, masing-masing perlakuan diulang sebanyak empat kali. Faktor yang diujikan adalah faktor P: usia korpus luteum 15 hari (P1) dan 10 hari (P2), faktor C: pencabutan atau pelepasan implan progesterone pada hari ke-9 (C1) dan pada hari ke-13(C2). Hasil yang diperoleh dengan uji statistik menunjukkan bahwa tidak terdapat interaksi antara perbedaan usia korpus luteum dengan perbedaan waktu pencabutan atau pelepasan implan controlled internal drug release (CIDR) (p>0.05). Dari total sampel 16 ekor sapi perah, 13 sampel menunjukkan tanda birahi tiga hari setelah implan controlled internal drug release (CIDR) dilepaskan. Persentase total dari keseluruhan sampel yang menunjukkan tanda birahi adalah 81.25%.
The Excellence of Performance, Semen Quality and Testosterone Levels on Bull in Teaching Farm, Faculty of Veterinary Medicine, Universitas Airlangga Pudji Srianto; Trilas Sardjito; Budi Utomo; Oky Setyo Widodo
Jurnal Medik Veteriner Vol. 4 No. 1 (2021): April
Publisher : Faculty of Veterinary Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol4.iss1.2021.131-136

Abstract

Study on performance, semen quality and testosterone levels of 2 young bulls (age range 3-5 years) Limousine and Simental and 2 old bulls (age range 6-8 years) types Limousin and Simental have been carried out in the Teaching Farm, Faculty of Veterinary Medicine, Universitas Airlangga. This study aimed to determine the performance, semen quality and testosterone levels can be used as a guideline whether or not the ejaculate of young and old bulls should be continued into frozen semen. Performance tests were carried out using measuring sticks and weight bands, semen quality tests included macroscopic and microscopic tests, semen concentration using a spectrophotometer and testosterone test using the Sandwich-ELISA method. The mean results of the performance test in young bulls were, while the mean for older bulls were: body weight 700 kg; hump height 136.5 cm; circumferens of chest 213 cm; body length 171 cm and scrotal circumference 35.5 cm. The performance test for old bulls is body weight 840 kg; hump height 143 cm; circumferens of chest 225.5 cm; body length 177.5 cm and scrotal circumference 40.25 cm. Mean macroscopic of semen examination for young bulls, mean volume 8 cc; milky white color; characteristic odor; The degree of acidity was 6.5 and mucous consistency, while for the old bulls the volume was 6.85; milky white color; characteristic odor; 6.5 degrees of acidity and mucous consistency. The mean microscopic examination for young bulls was mass movement ++ / +++; individual movement 80/3; concentration 745.5 million / ml; abnormalities 3% and 92.25% viability, for old bulls: movement mass ++ / +++; individual movement 77.5 / 3; concentration 1291.5 millon / ml; abnormalities 5% and viability 90%. Examination of serum testosterone levels using the Sandwich-ELISA technique was obtained by an average of 1.299 ± 0.1598 ng/ml in young bulls and 1.101 ± 0.1502 ng/ml in older bulls. This study concluded that young bulls and old bulls in the Teaching Farm, Faculty of Veterinary Medicine, Universitas Airlangga in terms of performance, semen quality and testosterone levels are suitable for processing into high quality frozen semen.
Kualitas semen sapi pejantan berdasarkan umur, suhu, dan kelembaban di Taman Ternak Pendidikan Universitas Airlangga Febby Fairy Brillianti; Pudji Srianto; Trilas Sardjito; Tri Wahyu Suprayogi; Indah Norma Triana; Dadik Rahardjo
Ovozoa : Journal of Animal Reproduction Vol. 10 No. 3 (2021): Ovozoa : Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v10i3.2021.81-89

Abstract

This research was conducted in the teaching farm of the Faculty of Veterinary Medicine, Universitas Airlangga. The present study aimed to determine the effect of age, temperature, and humidity on bull semen quality which was processed into frozen semen. Three age groups of bulls (young 3-4 years, middle age 5-6 years, and old 7-8 years) were collected for the temperature and humidity of semen examination. Fresh semen was collected using an artificial vagina, followed by macroscopic and microscopic examination. In addition, post-thawing motility was also evaluated. The results showed that there was no effect of temperature and humidity during semen collection process. Fresh semen quality was affected by age. Moreover, the post-thawing motility of fresh semen of spermatozoa was lower than those of the fresh semen; however, it still qualifies for artificial insemination. It could be concluded that the semen of bulls at the Teaching Farm of Universitas Airlangga has good quality, and is suitable for Artificial Insemination.
Antibiotic Resistance in Escherichia coli Isolated from Feces of Bali Cattle With Reproductive Disorders Kholik Kholik; Muhammad Munawaroh; Muhammad Rama Imam Saputra; Rahmawati Rahmawati; Pudji Srianto
Jurnal Biodjati Vol 6, No 2 (2021): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v6i2.13925

Abstract

Antimicrobial resistance (AMR) has become a global issue in animal, human and environmental health. The AMR profile of Escherichia coli reflects the use of antibiotics in production animals. The purpose of this study was to determine the antibiotic resistance of Escherichia coli bacteria isolated from the feces of female Bali cattle with reproductive disorders. Feces samples were taken purposively using a swab on 4 rectums from 7 Bali cattle with reproductive disorders in June 2021 in Lando Village, East Lombok Regency. Escherichia coli samples were isolated on Eosin Methylene Blue Agar media and identified by biochemical tests. An antibiotic resistance test against Escherichia coli was carried out by the disk diffusion method. The antibiotics used in the test were Penicillin G 10 U, Oxytetracycline 30 g, Gentamicin 10 g, and Tetracycline 30 g, and Cefotaxime 30 g. The results of planting on Eosin Methylene Blue Agar media obtained 4 Escherichia coli which were successfully isolated from 4 samples of Bali cattle feces. Data on the level of Escherichia coli susceptibility level to various antibiotics were analyzed using the chi-square test. The results of the susceptibility test to antibiotics showed that 4 samples of Escherichia coli (100%) were resistant to Penicillin G, (25%) were resistant to Oxytetracycline, (25%) were resistant to Cefotaxime, and (100%) samples of Escherichia coli were sensitive to Gentamicin and Tetracycline. The chi-square test on the level of Escherichia coli susceptibility to various antibiotics was significant with p˂ 0.05 (p-value = 0.012). The results of this study have shown that Escherichia coli from Bali cattle feces experience multidrug resistance which later on might have an impact on human health and the environment.
THE EFFECT OF UREA SUPLEMENTATION IN MATURATION MEDIA OF BOVINE OOCYTE IN VITRO TOWARDS EXPRESSION OF BAX, BCL-2 AND BAX/BCL-2 RATIO Dhesy Kartikasari; Sri Mulyati; Suzanita Utama; Pudji srianto; Widjiati Widjiati; Hani Plumeriastuti
Jurnal Kedokteran Hewan Vol 14, No 1 (2020): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v14i1.15283

Abstract

This study aims to evaluate the expression of BAX, BCL-2, and BAX/BCL-2 ratio in maturation media of cow oocytes which supplemented with Urea in vitro because BAX and BCL-2 are the main regulators of apoptosis. A total of 263 oocytes from follicle aspirations originating from ovaries taken from slaughterhouses and were saturated with 3 addition of urea which was divided into three groups. The control group (P0) was control group without the addition of urea, P1 group was added with urea 20 mg/dL, while P2 group was added with urea 40 mg/dL. The results of in vitro oocyte maturation were continued with identification using immunocytochemical staining with the addition of BAX and BCL-2 antibodies. Positive results showed a brownish color on the oocyte and its cumulus. The results of this study indicated that there were significant differences (P0.05) in BAX and BCL-2 expression, although both curves were equally increase. The increase in BCL-2 was more significant than BAX, while the BAX BCL-2 ratio did not show a significant difference (P0.05) in whichthe curve of BAX/BCL-2 ratio was decreased. It can be concluded that the addition of urea does not affect the level of apoptosis.
Persentase Motilitas dan Viabilitas Spermatozoa Kambing Sapera dalam Pengencer Tris Kuning Telur dan Susu Skim Kuning Telur Before Freezing Herlina Masyitoh; Tri Wahyu Suprayogi; Ratih Novita Praja; Pudji Srianto; Sri Pantja Madyawati; Amung Logam Saputro
Jurnal Medik Veteriner Vol. 1 No. 3 (2018): October
Publisher : Faculty of Veterinary Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (417.557 KB) | DOI: 10.20473/jmv.vol1.iss3.2018.105-112

Abstract

Penelitian ini bertujuan untuk mengetahui perbedaan persentase motilitas dan viabilitas semen kambing Sapera terhadap evaluasi semen segar dan before freezing dengan penambahan pengencer tris kuning telur dan susu skim kuning telur. Metode penelitian ini adalah pengambilan semen, evaluasi semen segar (pemeriksaan makroskopis dan mikroskopis), pengenceran semen, gliserolisasi, ekuilibrasi dan evaluasi before freezing. Penelitian ini terdiri dari P0, P1 dan P2, masing-masing perlakuan terdiri dari sembilan ulangan. P0 adalah semen segar kambing Sapera, P1 adalah semen kambing Sapera+pengencer tris kuning telur dan P2 adalah semen kambing Sapera+pengencer susu skim kuning telur. Data yang diperoleh dengan Uji T2 sampel dependent, membandingkan P0 dan P1 serta P0 dan P2. Data menunjukkan perbedaan sangat nyata (p<0,01) pada perlakuan P0 dan P1 persentase motilitas 76,11%; 65,56% dan viabilitas 82,33%;72,22%. P0 dan P2 persentase motilitas 76,11%;61,11% dan viabilitas 82,33%;67,78%. Hasil tersebut menunjukkan bahwa penelitian persentase motilitas dan viabilitas spermatozoa kambing Sapera mengalami penurunan dari semen segar pada tris kuning telur dan susu skim kuning telur before freezing.
Efektifitas Daya Pisah Electric Separating Sperm (ESS) terhadap Spermatozoa Kromosom X dan Y pada Kambing Sapera Fahlevi Lailiyah; Pudji Srianto; Amung Logam Saputro; Sri Pantja Madyawati; Bodhi Agustono; Ragil Angga Prastiya
Jurnal Medik Veteriner Vol. 1 No. 3 (2018): October
Publisher : Faculty of Veterinary Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (399.223 KB) | DOI: 10.20473/jmv.vol1.iss3.2018.93-98

Abstract

Penelitian ini bertujuan untuk mengetahui efektifitas pemisahan alat Electric Separating Sperm (ESS) terhadap spermatozoa kromosom X dan Y pada sisi anoda dan katoda dengan variabel meliputi presentase viabilitas, motilitas, abnormalitas dan morfometri spermatozoa. Metode pengambilan semen menggunakan vagina buatan, kemudian dilakukan pemeriksaan makroskopis dan mikroskopis, apabila menunjukkan hasil yang baik dilanjutkan dengan pengenceran semen dengan Tris Kuning Telur dan dilakukan pemisahan. Terdapat tiga perlakuan pada penelitian ini yaitu P1 (3 menit), P2 (7 menit), P3 (10 menit) dengan enam kali pengulangan pada sisi anoda dan katoda. Data pada tiap variabel (viabilitas, motilitas dan abnormalitas) diperiksa menggunakan analisis statistik ANOVA dan untuk melihat adanya pemisahan spermatozoa kromosom X dan Y dilakukan pengukuran terhadap kepala menggunakan morfometri. Hasil pemisahan tertinggi yaitu pada P3 dengan perbandingan pemisahan X : Y sejumlah 65,00:35,00 pada sisi katoda, kemudian pada sisi anoda persentase perbandingan X : Y yaitu 59,00:41,00. Hasil kualitas spermatozoa setelah pemisahahn menunjukkan presentase viabilitas terbaik terdapat pada P1 yaitu 75,00a±2,00 pada sisi anoda dan 76,50a±2,17 pada sisi katoda. Motilitas spermatozoa P1 menunjukkan motilitas progresif spermatozoa dengan rataan 60,83a±2,04 pada anoda dan 61,67a±2,58 pada sisi katoda. Presentase abnormalitas tidak menunjukkan perbedaan yang signifikan antar perlakuan.
Detection of Fertility Levels of Female Bawean Deer (Axis kuhlii) Based on Fecal Steroid Metabolic Mitha Ardila Rahmawati; Mas’ud Hariadi; Tjuk Imam Restiadi; Rimayanti Rimayanti; Tita Damayanti Lestari; Pudji Srianto
Jurnal Medik Veteriner Vol. 4 No. 1 (2021): April
Publisher : Faculty of Veterinary Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol4.iss1.2021.84-90

Abstract

The aim of the study was to detect the fertility level of females Bawean deer (Axis kuhlii) through fecal steroid metabolic which were tested using Enzyme-Linked Immunosorbent Assay (ELISA). The study used five females Bawean deer in Taman Flora Surabaya, which were marked using R1, R2, R3, R4, and R5 necklaces. The feces of each deer was collected on the 1st day, 6th day, 11th day, 16th day, and 21st day. The samples which were collected then extracted using the freeze dry method. Samples were tested using ELISA. The results of the measurement of the levels of fecal steroid metabolic  in five Bawean deers showed that three Bawean deer (R1, R4, and R5) were in the luteal or pregnant phase as indicated by the increased of progesterone from 1st day to 21st day. While the other two Bawean deer (R2 and R3) were in the follicular or estrous phase as indicated by the decreased of level of faecal steroid metabolic  on 11th day for deer R2 and 16th day for deer R3. The results showed that the levels of the fecal steroid metabolic can be used to determine the follicular phase and luteal phase and the pregnancy status of female Bawean deer.
The Steroid Hormone Profile in Etawah Crossbreed Goat While Ovulation Induced using The Selectsynch Method Muhammad Aftabuddin Rz; Pudji Srianto; Chairul Anwar Nidom; Sri Pantja Madyawati; Trilas Sardjito; Ismudiono Ismudiono
Jurnal Medik Veteriner Vol. 5 No. 1 (2022): April
Publisher : Faculty of Veterinary Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol5.iss1.2022.62-68

Abstract

This study aimed to provide alternative information and solutions in an effort to increase reproductive productivity in etawah crossbreed goats (PE). The sample used in this study was 10 female PE with an average age of 2.5 - 3 years and primiparous at least . Ovulation induction was performed using 0.1 mg intra-muscular Gonadotropin-Releasing Hormone (GnRH), after seven days injection of Prostaglandin F2α (PGF2α) was given as much as 2.5 mg submucosa of the vulva, followed by a second injection of GnRH as much as 0,2 mg intramuscular in samples that have really experienced heat and selected selectively according to the signs of natural heat shown by the sample and followed by insemination as much as 2 doses or 0.50 ml of frozen semen. Blood sampling was performed at H0, H7th, H14th and H21th after insemination. All blood samples were collected and progesterone and estrogen hormone profiles were examined using the ELISA method. From the results of the ELISA test, the mean progesterone hormone profile H0 = 4.798 ng / ml, H7th = 4.887 ng / ml, H14th = 4.824 ng / ml, H21th = 5.148 ng / ml. The profile of the hormone estrogen at H0 = 19,461 pg / ml, H7th = 17,457 pg / ml, H14th = 18,248 pg / ml, H21th = 17,515 pg / ml. This study showed an increase in the levels of the progesterone hormone at H0 to H7th, then slightly decreased in H14th and a significant increase in H21th. For the estrogen hormone, there is a decrease from H0 to H7th then there is a slight increase in H14th and decreases again in H21th.
KEJADIAN KAWIN BERULANG PADA SAPI POTONG BETINA PERANAKAN LIMOSIN DAN SIMENTAL DI KECAMATAN WONOSALAM KABUPATEN JOMBANG PERIODE 2015 - 2018 Muharam Subagio; Indah Norma Triana; Bambang Poernomo; Wurlina Wurlina; Pudji Srianto; Budi Utomo
Journal of Basic Medical Veterinary Vol. 8 No. 2 (2019): Journal of Basic Medical Veterinary, December 2019
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v8i2.20412

Abstract

Repeat Breeding is an adult cattle which have normal estrous cycle and clear signs of estrous but did not get pregnant when it got breed by natural mating or artificial insemination. The main purposes of this study is to know the number of repeat breeding cases in beef cattle in regency Jombang period of 2015-2018. The metode of the study is survey which collected by recording and survey. Were analyzed though Normality Test (Kolmogorov-Smirnov Test) and Homogenity Test and followed by T Test. Questionnaire test result processed using Kendall’s Tau-b Correlation Test. Study result showed that repeat breeding cases fluctuate significantly per year and the factors affecting fertilization failure and early embrionic death, hormonal, infection, and management factors.