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Efek pemberian α-tokoferol terhadap jumlah sel spermatogenik dan sel Leydig pada tikus putih (Rattus norvegicus) yang dipapar 2,3,7,8 Tetrachlorodibenzo-p-dioxin Ainun Machmudia; Hana Eliyani; Widjiati Widjiati; Wurlina Wurlina
Ovozoa : Journal of Animal Reproduction Vol. 10 No. 3 (2021): Ovozoa : Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v10i3.2021.72-80

Abstract

 This research aimed to determine the effect of α-tocopherol on the count of spermatogenic and Leydig cells in rats (Rattus norvegicus) exposed to 2,3,7,8 tetrachlorodibenzo-p-dioxin (dioxin). Dioxin is an endocrine-disrupting agent that adversely affects reproductive health, while α-tocopherol maintains fertility. This research used 25 rats aged 10-12 weeks weighing 150-200 grams. Rats were divided into five groups (K, P0, P1, P2 and P3). The K (control group) was administered with corn oil 1 ml/day. P0 was exposed to 700 ng/kg/day dioxin. P1, P2 and P3 was exposed to dioxin at a dose of 700 ng/kg/day and administered with α-tocopherol at a dose of 77, 140 and 259 mg/kg/day respectively. Dioxin exposure, corn oil and α-tocopherol administration were conducted orally for 20 days. On day-21, all rats were sacrificed for histological slides preparation of testicles with hematoxylin-eosin staining. Data were analyzed with analysis of variance and continued with the Duncan test. The results indicated that exposure to dioxin caused a decrease in the number of spermatogenic and Leydig cells. The administration of α-tocopherol at a dose of 140 mg/kg/day eliminated the effect of reducing the number of spermatogenic and Leydig cells caused by exposure to dioxin. The conclusion was the administration of α-tocopherol at 140 mg/kg/day was effective in maintaining the number of spermatogenic and Leydig cells in rats (Rattus norvegicus) exposed to 2,3,7,8 tetrachlorodibenzo-p-dioxin.
PENGARUH ANTIMITOSIS EKSTRAK Achyranthes Aspera Linn PADA PEMBELAHAN SEL EMBRIO (CLEAVAGE) Wurlina Wurlina
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 11 No 2 (2006): June 2006
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/387

Abstract

To study the antimitotic effect of an extract of the leaf Achyranthes asapera Linn on embryonal cleavage rats (Rattus norwegicus). Rats ova were fertilized in vitro, and thes suitable zygote and embryo were selected to use as samples, which were divided into 5 groups. Each group comprised 80 ova/embryo. Those groups were: group 1. ova for fertilization, group 2. zygote, group 3. 2 cell embryo, group 4. 4 cell embryo and group 5. 8 cell embryo. Each group was divided into 4 treatmens, each of which consisted of 20 ova/ embryos. The treatments were as follows: control treatment receiving TCM 199 media, and treatment 1, 2 and 3 receiving Achyranthes aspera Linn alkaloid of 20, 30, and 40 ppm respectively. Observation to embryonal cleavage and development was out 12 and 24 hours after treatment. Concluded from the results that the administration of Achyranthes aspera Linn as much as 30 ppm in vitro could 1) inhibits fertilization zygote and 2, 4, and 8 cell embryonal cleavage, growth and development 2) induced zygote and embryonal membrane recruitment and blastomere degeneration and 3) inhibits zygote and embryonal mitotic cleavage at metaphase stage.
PEMBIBITAN DAN PENGGEMUKAN KAMBING “LOKETAWA” PENGHASIL DAGING DAN SUSU RAKITAN TEKNOBREEDING DAN TEKNOFATTENING PAKAN TANPA HIJAUAN (COMPLETE FEED) Wurlina Wurlina; Rimayanti Rimayanti; Mas'ud Hariadi; Dewa Ketut Meles
Jurnal Layanan Masyarakat (Journal of Public Services) Vol. 1 No. 1 (2017): Jurnal Layanan Masyarakat
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (204.34 KB) | DOI: 10.20473/jlm.v1i1.2017.46-50

Abstract

IbM to business gorup in the goat breeding and fattening “Loketawa” aims to: 1) improve local goat genetics through IB using Etawa stud 2) obtain mother goats lust together done synchronization lusts 3) get mother goat bunting from once a year to 2 times a year 4) increase the number of children from 1 birth to 3-4 births using superovulation techniques 5) reduce production costs, make feed without forage and growth promotor. The method of implementation used 1) introducing stud Etawa meat and milk producers 2) synchronization lust using PGF2α 3) superovulation using hormone FSH and LH 4) IB on goats using Etawa goat cuttings, 5) the processing of feed without forage and growth promoter. The results are as follows: 1) as many as 20 heads of goats are simultaneously using 100% PGF2α, artificial insemination on 10 heads of goats without super ovulation has an average child of 1.6 births, while artificial insemination on 10 goats with super ovulation having an average child 3 births, 3) an average goat weight increase of 252.35 grams per day. AbstrakIpteks bagi Masyarakat yang dilakukan pada kelompok usaha pembibitan dan penggemukan kambing “Loketawa” bertujuan: 1) memperbaiki genetik kambing lokal melalui IB menggunakan pejantan Etawa 2) mendapatkan induk kambing birahi bersamaam dilakukan sinkronisasi birahi 3) mendapatkan induk kambing bunting dari setahun sekali menjadi 2 kali setahun 4) meningkatkan jumlah anak dari 1 ekor sekelahiran menjadi 3-4 ekor sekelahiran menggunakan teknik superovulasi 5) menekan biaya produksi, membuat pakan tanpa hijauan dan growth promotor. Metode pelaksanaan yang digunakan adalah 1) memperkenalkan pejantan Etawa penghasil daging dan susu 2) sinkronisasi birahi menggunakan PGF2α 3) superovulasi menggunakan hormon FSH dan LH 4) IB pada kambing menggunakan semen kambing Etawa, 5) pengolahan pakan tanpa hijauan dan growth promoter. Hasilnya adalah sebagai berikut : 1) sebanyak 20 ekor induk kambing mengalami birahi bersamaan menggunakan PGF2α sebesar 100%, Inseminasi buatan pada 10 ekor induk kambing tanpa super ovulasi mempunyai anak rata-rata 1,6 ekor sekelahiran, sedangkan Inseminasi buatan pada 10 ekor induk kambing dengan super ovulasi mempunyai anak rata-rata 3 ekor sekelahiran, 3) peningkatan berat badan kambing rata- rata 252,35 gram/ekor perhari.
Penggemukan sapi menggunakan pakan tanpa hijauan (complete feed) di era pandemi covid-19 (Fattening of cow cattle using green-free feed (complete feed) in the covid-19 pandemic era) Dewa Ketut Meles; Imam Mustofa; W Wurlina; Mas’ud Hariadi
Buletin Pengabdian: Bulletin of Community Services Vol 1, No 2 (2021): AUGUST
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (448.733 KB) | DOI: 10.24815/bulpengmas.v1i2.21640

Abstract

Problems in the COVID-19 pandemic era for cows’ cattle farmers were limited skills of farmers in processing of feed without forage in order to reduce production costs and anticipate prolonged droughts. The government's steps to overcome these problems are by providing business capital assistance, deferring loan payments, and assisting cows cattle farmers. The Regional Superior Product Development Program (RSPD) aims to determine the increase in body weight of various types of cattle using feed without forage and growth promoters in the COVID-19 pandemic era of SMEs of cows cattle fattening. Data was collected by interview/questionnaire methods and making feed without forage, growth promoter, and fermentor. The feed from RSPD was applied to five types of cows’ cattle (local cattle, ongole crossbreeds, madura cattle, limousin crossbreeds and Simmental crossbreeds). This activity was carried out from February until June 2021. The results showed that farmer’s skill in making feed without forage, growth promoters, and fermenters has been improved and also type of cows’ cattle with good weight gain has been determined. In conclusion, the production of feed without forage, growth promoters, and fermentor in the COVID-19 pandemic era could be done by the cattle farmers independently. Limousin and Simmental crossbreeds had good weight gain compared to local cattle, ongole crossbreeds and madura cattle.
PENGGEMUKAN SAPI MENGGUNAKAN PAKAN TANPA HIJAUAN (COMPLETE FEED) DAN TAPE JERAMI SERTA GROWTH PROMOTOR STRATEGI PENCAPAIAN SWASEMBADA DAGING Wurlina Wurlina; Mas’ud Hariadi; Imam Mustofa; Dewa Ketut Meles
Jurnal Layanan Masyarakat (Journal of Public Services) Vol. 2 No. 2 (2018): Jurnal Layanan Masyarakat
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (221.341 KB) | DOI: 10.20473/jlm.v2i2.2018.63-68

Abstract

Program Kemitraan Masyarakat (PKM) which performed on a group of cattle fattening is not qualified yet to solve the problems 1) rice straw as animal feed 2) agricultural waste and industrial waste as feed without forage 3) processing of rice straw into straw tape 4) processing agricultural waste and industrial waste into animal feed without forage 5) processing growth promotor derived from empon-empon and retrieval medicinal plants, and 6) making of cow manure waste into environmentally friendly fertilizer. The purposes of PKMare (1) to process of rice straw into straw tape as a nutritious animal feed (2) without forage feed processing (complete feed) (3) processing of growth promotors (4) local cattle fattening using tape straw and feed without forage as well as growth promotor. Benefits of PKM is to develop human resources and increase income of fattening local cattle farmers with lower production costs. The method used in PKM activities are education and training of local cattle fattening using tape straw feed and feed without forage plus growth promotor, processing waste into environmentally friendly fertilizer on UKMK of local cattle fattening members in Tuban. The result is enhancement of cattle weight per cow per day on local cattle amounted to 0.625 ± 0.048 kg, ongole rossbreed cattle 0.924 ± 0.067 kg, hybrid limousine cattle for 1.673 ± 0.068 kg and Simmental crossbreed cattle of 2.105 ± 0.414kg.AbstrakProgram Kemitraan Masyarakat (PKM) yang dilakukan pada kelompok penggemukan sapi belum memenuhi syarat untuk menyelesaikan masalah 1) jerami padi sebagai pakan ternak 2) limbah pertanian dan limbah industri sebagai pakan tanpa hijauan 3) pengolahan jerami padi menjadi pita jerami 4) pengolahan limbah pertanian dan industri limbah menjadi pakan ternak tanpa hijauan 5) promotor pertumbuhan pengolahan berasal dari empon-empon dan pengambilan tanaman obat, dan 6) pembuatan kotoran sapi menjadi pupuk ramah lingkungan. Tujuan dari PKM adalah (1) untuk memproses jerami padi menjadi pita jeramisebagai pakan ternak bergizi (2) tanpa pengolahan pakan hijauan (pakan lengkap) (3) pengolahan promotor pertumbuhan (4) penggemukan sapi lokal menggunakan pita jerami dan pakan tanpa hijauan serta promotor pertumbuhan. Manfaat PKM adalah mengembangkan sumber daya manusia dan meningkatkan pendapatan penggemukan sapi lokal dengan biaya produksi lebih rendah. Metode yang digunakan dalam kegiatan PKM adalah pendidikan dan pelatihan penggemukan sapi lokal menggunakan tape jerami pakan dan pakan tanpa hijauan ditambah promotor pertumbuhan, pengolahan limbah menjadi pupuk ramah lingkungan pada UKMK anggota penggemukan sapi lokal di Tuban. Hasilnya adalah peningkatan berat sapi per sapi per hari pada sapi lokal sebesar 0,625 ± 0,048 kg, sapi peranakan ongole 0,924 ± 0,067 kg, sapi limusin hibrida untuk 1,673 ± 0,068 kg dan sapi potong silang Simmental sebesar 2,105 ± 0,414 kg.
THE ROLE OF ACUPUNCTURE THERAPY TO OVERCOME ONE-SIDE HEAD PAIN (MIGRAINE) Mut Mainnah; Maya Septriana; Wurlina Wurlina
Journal of Vocational Health Studies Vol. 5 No. 3 (2022): MARCH 2022 | JOURNAL OF VOCATIONAL HEALTH STUDIES
Publisher : Faculty of Vocational Studies, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jvhs.V5.I3.2022.192-202

Abstract

Background: Migraine is a primary headache, usually described as pain attacks lasting 4 - 72 hours, unilateral, throbbing, with moderate to severe pain intensity that can be exacerbated by activity, and can be accompanied by nausea and vomiting, photophobia and phonophobia. Migraine can interfere with patient activities, so that it can reduce productivity and result in an economic burden to the family and reduce the patient's quality of life. To handle it, pharmacological and non-pharmacological therapies can be used. Acupuncture therapy is one of the non-pharmacological therapeutic options that can be used in migraines. Purpose: This article review collects and analyzes journals related to the effect of acupuncture therapy on migraines. The journal search method was carried out electronically from December to April 2021 using several databases such as Pubmed, Sagepub and Google Schoolar with the keywords: acupuncture in cases of migraine, acupuncture for migraine, and acupuncture point for migraine. Review: The journal searches discovered that acupuncture therapy had an effect in reducing pain intensity in migraine cases with the most widely used method that was the manual acupuncture method. However, the most influential method was the combination method of manual acupuncture, herbal acupuncture, and nutritional therapy with the hyperactivity of Yang liver as the frequently occurring syndrome. The frequently used points were the acupuncture points of Fengchi-GB20, Hegu-LI4, Taichong-LR3, Baihui-DU20, Zusanli-ST36, Sanyinjiao-SP6, and Taiyang-EX-HN5.Conclusion: Based on the journal analyses, the combination method of manual acupuncture, herbal acupuncture, and nutritional therapy can reduce pain intensity in migraine cases
Efek Antimitogenik Fraksi Alkaloid Achyranthes aspera Linn. terhadap Induksi Apoptosis pada Mencit yang Terinfeksi Mycobacterium tuberculosis Dewa Ketut Meles; . Wurlina; I Dewa Putu Anom Adnyana; Sunarni Zakaria; Dewa Made Sucipta Putra; Niluh Suasanti
Acta VETERINARIA Indonesiana Vol. 3 No. 1 (2015): Januari 2015
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (312.499 KB) | DOI: 10.29244/avi.3.1.8-15

Abstract

Tujuan dari penelitian ini adalah untuk mendeteksi efek antimitogenik dari ekstrak fraksi alkaloid daun Achyranthes aspera Linn. terhadap induksi apoptosis, pertumbuhan dan perkembangan sel paru-paru yang diinfeksi oleh Mycobacterium tuberculosis. Efek antimitogenic dari alkaloid A. aspera diujikan pada 120 ekor mencit yang diinfeksikan dengan 100 sel/mL M. tuberculosis dan dibagi menjadi 6 kelompok sehingga masing-masing kelompok terdiri dari 20 ekor. Kelompok perlakuan terdiri dari: kontrol negatif dengan tikus sehat yang diberikan adjuvant saja, kontrol positif dengan diberikan rifampisin 600 mg/kgbb/hari, dan kelompok perlakuan P0 , P1 , P2 , dan P3 yang diinfeksi M. tuberculosis dan diberikan alkaloid dengan dosis bertingkat 0, 60, 12, dan 180 mg/kgbb/po/hari selama 30 hari. Parameter pengamatan terdiri dari: jumlah total leukosit, jumlah total jenis leukosit, jumlah karbuncel di paru-paru, dan jumlah sel paru-paru yang mengalami apoptosis. Hasil penelitian menunjukkan pada tikus yang terinfeksi M. tuberculosis mengalami penurunan jumlah total dan jenis leukosit (eosinofil, neutrofil, lmfosit dan monosit), dan jumlah karbunkel di paru-paru pada kelompok perlakuan akaloid mulai dosis 60 mg/kgbb. Jumlah sel di paru-paru yang mengalami apoptosis juga mengalami penurunan pada kelompok pemberian akaloid daun A. aspera mulai dosis 60 mg/kgBB sama dengan kelompok rifampisin. Kesimpulan dari penelitian bahwa alkaloid daun A. aspera menyebabkan penurunan jumlah total dan jenis leukosit, serta jumlah karbuncel dan sel paru yang mengalami apoptosis pada tikus yang terinfeksi oleh M. tuberculosis.Kata kunci: Achyranthes aspera Linn., antimitogenik, paru-paru tuberkulosis, apoptotis, leukosit (The Antimitogenic Effect of Alkaloid Fraction of Achyranthes aspera Linn. on Apoptotic Induction in Mice Infected by Mycobacterium tuberculosis)The aims of this research was to determine the antimitogenic effect of alkaloid Achyranthes aspera Linn. on apoptotic induction, growth and cell development in lung cell infected by Mycobacterium tuberculosis. Antimitogenic effect of alkaloid A. aspera was tested in 120 mice that infected with 100 cell/mL M. tuberculosis and divided into 6 groups so that each group consist of 20 mice. The treatment groups were: negative control that healthy mice was given adjuvans only, positive control that was given rifampisin 600 mg/kg bb/day, and treatment group of P0 , P1 , P2 and P3 infected by M. tuberculosis and given alkaloid with dose 0, 60, 12 and 180 mg/kgbw/po/day continously during 30 days. The parameter of observation were total leucocyte count, total differential leucocyte count, total carbuncel in lung, and percentage of apoptotic lung cells. The result showed that mice infected by M. tuberculosis have decreased in total leucocyte and diferentiated leucocyte total (eosinophil, neutrophil, lymphocyte, and monocyte), and total carbuncel in lungs after treated by akaloid A. aspera with dose begin 60 mg/kgbw. Apoptotic cell in lung was also decreased in the group tretaed by akaloid A. aspera with dose begin 60 mg/kgbw that the value was equal to the group of rifampisin. In conclusion, treatment of alkaloid from A. aspera caused depreciation in leucocyte total and leucocyte differentiation, and total of carbuncel and apoptotic cell in the lung in mouse that infected by M. tuberkulosis.Keywords: Achyranthes aspera Linn., antimitogenic, tuberculosis lung, apoptotic, leucocyte 
Efek Anti Diabetes Buah Pare (Momordica charantia Linn.) Terhadap Kadar Glukosa Darah, Sel Penyusun Pulau Langerhans dan Sel Leydig pada Tikus Putih Hiperglikemia I Dewa Putu Anom Adnyana; Dewa Ketut Meles; . Wurlina; Sunarni Zakaria; Niluh Suwasanti
Acta VETERINARIA Indonesiana Vol. 4 No. 2 (2016): Juli 2016
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (880.818 KB) | DOI: 10.29244/avi.4.2.43-50

Abstract

Penelitian ini bertujuan untuk membuktikan ekstrak buah pare (Momordica charantia Linn.) terhadap kadar glukosa darah, sel penyusun pulau Langerhans dan sel Leydig tikus putih (Rattus norvegicus) hiperglikemia. Tikus putih sebanyak 25 ekor dibagi secara acak menjadi 5 kelompok. Induksi aloksan dengan dosis 150 mg/kgbb secara intraperitoneal untuk menimbulkan kerusakan pankreas dilakukan pada 5 kelompok perlakuan. Tiga kelompok perlakuan diterapi dengan berbagai dosis ekstrak buah pare, (P1) 29 mg/1ml/hari, (P2) 50 mg/1ml/hari, dan (P3) 59 mg/1ml/hari, satu kelompok sebagai kontrol negatif (P0) diberi CMC Na 0,5% 1ml/hari, kontrol positif (K+) diberi Glibenclamide® 0,126 mg/1ml/hari. Ekstrak buah pare diberikan selama 21 hari. Kadar glukosa diperiksa setelah 2 jam, 4 jam, 6 jam dan 8 jam pascapemberian dihari pertama. Kadar glukosa selanjutnya diperiksa pada hari ke 7, 14 dan 21. Semua tikus dieuthanasia setelah 21 hari perlakuan, pankreas dan testis diambil untuk dibuat preparat histopatologi. Hasil menunjukkan bahwa ekstrak buah pare (Momordica charantia Linn.) memiliki efek antidiabetik yang dapat menurunkan kadar gula darah, meningkatkan jumlah sel insula Langerhans dan meningkatkan jumlah sel Leydig pada dosis 50 mg/1ml/hari pada hari ke 21 setelah perlakuan.
IDENTIFIKASI GROWTH DIFFERENTIATION FACTOR-9 (GDF-9) DARI MATURASI IN VITRO OOSIT SAPI DENGAN TEKNIK IMUNOSITOKIMIA Widjiati w; Desi Wulansari; Wurlina w; Ngakan Made Rai Widjaja
Jurnal Kedokteran Hewan Vol 5, No 2 (2011): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v5i2.356

Abstract

Penelitian ini bertujuan mengindentifikasi GDF-9 di dalam oosit yang berasal dari preantral folikel sapi yang dimatangkan secara in vitro. Penelitian ini menggunakan metode imunositokimia ikatan antara molekul avidin dan biotin yang terkandung dalam antibodi sekunder bentuk kompleks avidin-biotin. Ovarium sapi diambil dari rumah potong hewan (RPH) Pegirian Surabaya. Penelitian ini menggunakan 105 oosit matang yang berasal dari 62 ovarium. Hasil imunositokimia menunjukkan bahwa 77,14% GDF-9 teridentifikasi.
Pemulihan dan pengembangan ekonomi kreatif olahan hasil laut di Pesisir Bulak Surabaya pada era new normal untuk menunjang pertumbuhan ekonomi (Recovery and development of creative economy processed sea products in coastal Bulak Surabaya in new normal era for supporting economic growth) W Wurlina; Kadek Racmawati; Suzanita Utama; Gunanti Mahasri; Niluh Suwasanti; Desak Ketut Sekar Cempaka Putri
Buletin Pengabdian: Bulletin of Community Services Vol 1, No 1 (2021): APRIL
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (358.172 KB) | DOI: 10.24815/bulpengmas.v1i1.20241

Abstract

The Regional Prominence Product Development Program (RPPDP) aims to determine the impact of the coronavirus disease 2019 (COVID-19) pandemic on Micro, Small, and Medium Enterprises (MSMEs) based on the creative economy of seafood culinary in Bulak, Surabaya. This program was conducted to find out the solution that can be taken by government to recover and develop the creative economy of seafood culinary entrepreneur due to the COVID-19 pandemic impacts. Data were collected by interview/questionnaire, literature review, and implementation of recovery of the the creative economy of seafood culinary. The period of this study was from March (the beginning of the COVID-19 pandemic in Indonesia) to the middle of December 2020. The results of RPPDP showed that there were two elements influenced the seafood culinary entrepreneur due to the COVID-19 pandemic, namely Human Resources and innovation processing and marketing products. The government can take steps to overcome these problems, such as providing business capital assistance, giving discounts on electricity bills, deferring loan payments, assisting MSMEs who closed their businesses as result of the pandemic to transform or start new businesses. In conclusion, innovation of seafood culinary during the pandemic should be in accordance with the market demand, innovating traditional souvenirs to be work from home snacks, and online marketing using non-cash payment.