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All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy)
Sarsidi Sastrosumarjo
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Agriculture, Biological Sciences & Forestry Economics, Econometrics & Finance

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Ketahanan 22 Genotipe Cabai (Capsicum spp.) terhadap Phytophthora capsici Leonian dan Keragaman Genetiknya Rahmi Yunianti; Sarsidi Sastrosumarjo; Sriani Sujiprihati; Memen Surahman; Sri Hendrastuti Hidayat
Indonesian Journal of Agronomy Vol. 35 No. 2 (2007): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (302.865 KB) | DOI: 10.24831/jai.v35i2.1318

Abstract

Laboratory and field experiments were carried out to analyze genetic diversity of 22 pepper genotypes (Capsicum spp.) and their resistance to Phytophthora capsici Leonian. Resistance screening was performed in plastic flats 72 cells. Inoculation was done on 28-day old pepper plant soon after watering by pipeting 5 ml of inoculum (105 zoospore/ml) at the base of each plant. P. capsici  isolate used in this experiment was TG01, identified as race 3 based on AVRDC differential pepper lines (PI 188478, PBC 602, PBC 137 and Early Calwonder).  The evaluation of pepper genotypes characteristic was conducted in the field.  Principle Component Analysis, Clustering Analysis and Biplot Analysis were used to analyze genetic diversity based on 37 characters. Result of resistance evaluation showed that two genotypes (C4 and C13) were identified as resistant, 7 genotypes (C2, C3, C5, C8, C10, C15, and C20) as slightly resistant, 6 genotypes (C7, C9, C17, C19, C21 and C27) as slightly susceptible, and 7 genotypes (C1, C11, C18, C28, C48, C64, and C65) as susceptible.  Based on genetic diversity analyzed, all genotypes could be divided into 4 clusters.  Cluster I consisted of 18 genotypes i.e. C1, C2, C3, C4, C5, C7, C8, C9, C10, C13, C14, C15, C17, C18, C19, C28, C64, and C65. Cluster II consisted of only 1 genotype i.e. C48.  Cluster III consisted of 2 genotypes (C20 and C21) which were characterized by the colour of corolla, corolla spot, and filament. Cluster IV consisted of 1 genotype (C27) which was characterized by fruit cross-sectional corrugation.   Key words :  Capsicum, resistance, Phytophthora capsici, genetic diversity, clustering.
Induksi Mutasi pada Stek Pucuk Anyelir (Dianthus caryophyllus Linn.) melalui Iradiasi Sinar Gamma Syarifah Iis Aisyah; Hajrial Aswidinnoor; Asep Saefuddin; Budi Marwoto; Sarsidi Sastrosumarjo
Indonesian Journal of Agronomy Vol. 37 No. 1 (2009): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (620.001 KB) | DOI: 10.24831/jai.v37i1.1396

Abstract

It has been a common knowledge that LD50 is commonly used in estimating optimal doses of gamma irradiation in a breeding program. This research was aimed at observing radiosensitivity of five carnation's genotypes to gamma irradiation, to find the LD50 of carnation's cuttings, and to obtain solid mutants from five numbers of Carnation.  For cuttings, carnation genotype number 10.8 was the most insensitive to gamma rays, whereas number 24.15 was the most sensitive one.  LD50 of carnation's cuttings were obtained around 49 -72 gray. There were 19 mutants produced from this treatment. The desired mutans were mostly produced from the treated 24.1 genotype whereas the character mutans were mostly observed in MV2 generation. Irradiation treatment on genotype 24.1 produced most stabile mutans while the less was in genotype 24.14.  The produced mutants were qualitatively different in colour and petal shape, and stabile till third generation.   Key words: LD50, gamma irradiation, induced mutation, carnation.
Co-Authors ASEP SAEFUDDIN Budi Marwoto HAJRIAL ASWIDINNOOR Memen Surahman Rahmi Yunianti SRI HENDRASTUTI HIDAYAT Sriani Sujiprihati Syarifah Iis Aisyah