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Evaluasi Karakter Agro-morfologi Jengger Ayam (Celosia cristata L.) pada Genotipe Mutan M3 Nella Angelina Simanjuntak; Syarifah Iis Aisyah; Waras Nurcholis
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 48 No. 1 (2020): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24831/jai.v48i1.29939

Abstract

Celosia cristata L. atau jengger ayam merupakan tanaman hias dengan bentuk yang unik. Peningkatan keragaman C. cristata melalui teknik hibridisasi terkendala karena bunganya berbentuk bulir dan berukuran kecil sehingga menyulitkan proses kastrasi dan isolasi organ reproduksi. Induksi mutasi merupakan salah satu cara yang dapat digunakan untuk meningkatkan keragaman. Tujuan penelitian ini adalah mendapatkan informasi keragaman karakter agro-morfologi pada 17 genotipe mutan M3 jengger ayam hasil induksi mutasi sinar gamma di kebun percobaan Sabisa Farm. Karakter kualitatif dan kuantitatif yang terkait dengan agro-morfologi diamati berdasarkan deskriptor Union for The Protection of New Varieties of Plants (UPOV) yang dimodifikasi. Analisis ragam dilakukan menggunakan SPSS 20.0, sedangkan analisis kemiripan dengan menggunakan PBSTAT-CL 2.1. Keragaman signifikan antar genotipe C. cristata (p < 0.05) terdapat pada karakter tinggi tanaman dan panjang bunga. Tiga kelompok berdasarkan karakter agro-morfologi terbentuk dari analisis kemiripan pada koefisien ketidakmiripan 32%. Hasil mengindikasikan bahwa karakter karakter agro-morfologi dapat digunakan untuk membedakan 17 genotipe mutan M3 jengger ayam yang diteliti, sehingga dapat digunakan untuk pengembangan penelitian selanjutnya dalam program pemuliaan tanaman. Kata kunci: analisis kemiripan, agro-morfologi, keragaman, pemuliaan mutasi
Sensitivitas dan Keragaan Tanaman Coleus sp. terhadap Mutasi Induksi Kimia Menggunakan Ethyl Methane Sulfonate (EMS) Aplikasi Cara Rendam dan Tetes Dia Novita Sari; Syarifah Iis Aisyah; dan Muhammad Rizal Martua Damanik
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 45 No. 1 (2017): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (525.703 KB) | DOI: 10.24831/jai.v45i1.13157

Abstract

Variant of Coleus sp. in Indonesia is very low. Induced mutation by Ethyl Methane Sulphonate (EMS) is able to increase variant of Coleus sp.. EMS is a mutagen that is effective and efficient in causing mutations. In mutation breeding, the high variant usually occurs around LC50 (Lethal Concentration). The objective of this study were (1) to obtain the LC50 value and sensivity of coleus plants, and (2) to evaluate the response of plant growth and to obtain new variance of coleus used soak and drops by EMS application. The experiment was arranged in randomized complete block design with three replications in each coleus. The materials used two Coleus sp. shoot cuttings i.e., C. amboinicus Lour. and C. blumei the purple/green color and the red. The concentration of EMS consisted of 0.00, 0.50, 0.75, 1.00, and 1.25% with application soak method (100 minutes) and drops (3 drops pipette). The results showed that the LC50 value of C. amboinicus Lour. 5.86% (soak). The LC50 value of C. blumei the purple/green color was 0.69% (soak), 0.82% (drops) and the red color 0.29% (drops), 0.89% (drops). C. blumei (the purple/green and red) was higher sensitivity rather than C. amboinicus Lour. There was an interaction between treatments on C. amboinicus Lour. whereas C. blumei (purple/green and red) had no interaction among treatments. C. amboinicus Lour. produced one putative mutant and C. blumei purple/green produced four putative mutants based on the morphology.Keywords: Coleus amboinicus Lour., Coleus blumei, LC50, putative mutan
Analisis komparatif kandungan metabolit pada daun mutan tanaman Torbangun (Plectranthus amboinicus (Lour.) Spreng.) Aisyah, Syarifah Iis; Rusmiyati, Henny; Sukma, Dewi; Damanik, Rizal; Nurcholis, Waras
AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian Vol 4 No 1 (2020): AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian
Publisher : Universitas Bangka Belitung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33019/agrosainstek.v4i1.109

Abstract

Torbangun (Plectranthus amboinicus) merupakan tanaman obat dari keluarga Lamiaceae. Pada masyarakat Batak, daun torbangun digunakan untuk meningkatkan produksi air susu. Keragaman kandungan fitokimia dapat ditingkatkan salah satunya dengan teknik iradiasi sinar gamma untuk program pemuliaan tanaman. Pada penelitian ini, metode metabolomik yang tidak tertarget digunakan untuk mengevaluasi profil senyawa pada bagian daun tanaman mutan dari iradiasi sinar gamma dan kontrol. Kandungan senyawa planlet mutan dan kontrol dianalisis dengan GC-MS. Data GC-MS dianalisis dengan kemometrik dengan menggunakan hierarchical cluster analysis (HCA). Keragaman kandungan kimia ditunjukkan oleh planlet mutan dan kontrol daun torbangun. Hasil analisa planlet kontrol torbangun menunjukkan terdapat lima senyawa utama diantaranya hydroxymethylfurfurole (35,15%), brevifolin (7,69%), 3-Dihydro-3,5-Dihydroxy-6-Methyl-4H-Pyran-4-one (DDMP) (13,13%), stigmasterol (6,51%) dan ferruginol (8.63%). Hasil analisa mutan torbangun memiliki kandungan senyawa DDMP (19,57%), neophytadiene (8,47%), linolenic acid (9,10%), ferruginol (7,61%), stigmasterol (14,14%) dan gamma.-sitosterol (7,08%). HCA menunjukkan tiga komponen senyawa yang berbeda antara planlet kontrol dan mutan yaitu kenaikan kandungan senyawa DDMP dan stigmasterol, serta penurunan kandungan hydroxymethylfurfurole pada mutan torbangun.
Adaptability of Mutant Genotypes of Artemisia (Artemisia annua L.) as Result Of Gamma Irradiation in Three Locations with Different Altitude Purnamaningsih, Ragapadmi; Lestari, Endang Gati; Syukur, Muhamad; Yunita, Rosa; Aisyah, Syarifah Iis; Firdaus, Rohim
AGRIVITA, Journal of Agricultural Science Vol 33, No 3 (2011)
Publisher : Faculty of Agriculture University of Brawijaya and Indonesian Agronomic Assossiation

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The objective of this study was to identify the adaptability of twelve artemisia mutant genotypes, which were planted in three locations with different altitude, as a result of gamma irradiation. Randomized Complete Block Design (RCBD) was applied in this research with three replications as blocks. The genotypes 1B, 1C, 1D, 2, 3, 4, 5A, 6B, 7A, 8, 14, 15 and two control genotypes as parent genotype from seed and from in vitro were used. The genotypes were planted in three different locations such as Mount Putri, Cianjur (1450 m above sea level), Pacet, Cianjur (950 m above sea level) and Cicurug, Sukabumi (540 m above sea level). Based on the method of postdictive and predictive success, the model used was AMMI2 which was able to explain up to 100% of interaction-influenced variation. The genotypes which were found stabile and adaptive in these three locations were 1B, 1C, 1D, 6B and 15. Genotypes 3 and 7A were adaptive specifically in Pacet area, 5A was adaptive for Gunung Putri while genotype 4 was for Cicurug only.Keywords: AMMI, Artemisia annua, mutant genotype, adaptability
Respon Kerapatan Stomata dan Kandungan Klorofil Padi (Oryza sativa L.) Mutan terhadap Toleransi Kekeringan Hasna Dama; Syarifah Iis Aisyah; Azri Kusuma Dewi; S Sudarsono
Jurnal Ilmiah Aplikasi Isotop dan Radiasi Vol 16, No 1 (2020): JUNI 2020
Publisher : BATAN

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17146/jair.2020.16.1.5689

Abstract

Perakitan varietas padi toleran cekaman kekeringan dengan teknik pemuliaan mutasi mampu meningkatkan keragaman genetik tanaman sehingga memberi peluang untuk mendapatkan genotipe mutan yang toleran dengan mengetahui respon kerapatan stomata dan klorofil daun pada suatu tanaman. Penelitian ini bertujuan untuk melihat hubungan antara kerapatan stomata dan kandungan klorofil dengan ketahanan terhadap kekeringan pada genotipe padi mutan. Sampel menggunakan salah satu genotipe, daun yang digunakan adalah daun kedua dari daun bendera, perhitungan jumlah stomata dilakukan pada luas bidang pandang 40x dengan perhitungan jumlah stomata dibagi dengan satuan luas bidang pandang dan untuk klorofil daun diamati menggunakan klorofil meter (SPAD). Hasil penelitian menunjukkan bahwa genotipe padi mutan menunjukkan ketahanan terhadap cekaman kekeringan dengan respon kerapatan stomata paling tinggi dan skor penggulungan daun terkecil.
Induksi Mutasi Tanaman Leunca (Solanum nigrum L.) untuk Meningkatkan Keragaman Kandungan Tanin Siti Hartati Yusida Saragih; Syarifah Iis Aisyah; dan Sobir
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 47 No. 1 (2019): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (632.512 KB) | DOI: 10.24831/jai.v47i1.19502

Abstract

Leunca (Solanum nigrum L. ) is one of the indigenous vegetables in Indonesia. The fruit of this vegetable is green and has bitter taste because of its tannin content. The objectives of this study were to get lethal dose 50 (LD50), diversity and to obtain candidates of mutant plants with high and low tannin content in young fruit and ripe fruit in leunca. The research was conducted at Pasir Kuda experimental station, Bogor; National Nuclear Energy Agency (BATAN), Jakarta; and postharvest laboratory of the Center for Tropical Horticulture Studies (PKHT IPB), Bogor, from December 2016 to July 2017. Leunca accession of SN 20 and SN 29 were irradiated using doses of 0, 100, 150, 200, and 250 Gy of gamma ray. The LD50 values for SN 20 and SN 29 were 171.944 Gy and 190.949 Gy, respectively. The analysis of tannin content was performed based on permanganate method by titration using potassium permanganate. The results showed that the highest tannin content of green stage fruit was found in SN20D3.5 with 200 Gy dose and the lowest was found in SN29D1.5 with 100 Gy dose. The highest tannin content of ripe fruit was found in SN20D4.6 with dose of 250 Gy and the lowest was found in SN20D3.1 with dose of 200 Gy. Recommended tannin content for leunca is the low tannin content.Keywords: diversity, gamma ray, indigenous vegetable, irradiation, LD50
Adaptability of Mutant Genotypes of Artemisia (Artemisia annua L.) as Result Of Gamma Irradiation in Three Locations with Different Altitude Muhamad Syukur; Endang Gati Lestari; Ragapadmi Purnamaningsih; Rosa Yunita; Syarifah Iis Aisyah; Rohim Firdaus
AGRIVITA, Journal of Agricultural Science Vol 33, No 3 (2011)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v33i3.76

Abstract

The objective of this study was to identify the adaptability of twelve artemisia mutant genotypes, which were planted in three locations with different altitude, as a result of gamma irradiation. Randomized Complete Block Design (RCBD) was applied in this research with three replications as blocks. The genotypes 1B, 1C, 1D, 2, 3, 4, 5A, 6B, 7A, 8, 14, 15 and two control genotypes as parent genotype from seed and from in vitro were used. The genotypes were planted in three different locations such as Mount Putri, Cianjur (1450 m above sea level), Pacet, Cianjur (950 m above sea level) and Cicurug, Sukabumi (540 m above sea level). Based on the method of postdictive and predictive success, the model used was AMMI2 which was able to explain up to 100% of interaction-influenced variation. The genotypes which were found stabile and adaptive in these three locations were 1B, 1C, 1D, 6B and 15. Genotypes 3 and 7A were adaptive specifically in Pacet area, 5A was adaptive for Gunung Putri while genotype 4 was for Cicurug only.Keywords: AMMI, Artemisia annua, mutant genotype, adaptability
MULTIPLIKASI IN VITRO ANGGREK HITAM (Coelogyne pandurata Lindl.) PADA PERLAKUAN KOMBINASI NAA DAN BAP Aisyah, Syarifah Iis; Sukma, Dewi; Kartiman, Roni; Purwito, Agus
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol 5, No 1 (2018): June 2018
Publisher : Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1006.134 KB) | DOI: 10.29122/jbbi.v5i1.2908

Abstract

In Vitro Multiplication of  Black Orchid (Coelogyne pandurata Lindl.) Using the Combination of NAA and BAPABSTRACTBlack orchid is an indigenous plant from Kalimantan, Indonesia. It becomes endangered because of forest over-exploitation and its low natural reproduction rate. Tissue culture is considered to offer a solution to conserve and propagate this species. The aim of this research was to evaluate the effect of Naphtalene Acetic Acid (NAA) and 6-Benzile Amino Purine (BAP) on shoots multiplication of black orchid. The basic medium used was a half of Murashige & Skoog (MS) composition supplemented with 150 mLL-1 coconut water. Initial explants used were 6-month-old shoots of germinating seeds. The shoot cultures were incubated for 23 weeks. Results showed that the best combination for shoot multiplication was NAA 0.0 mgL-1 with BAP 0.2 mgL-1. Shoot grew better on medium with BAP and without NAA while roots growth was better on medium without the two plant growth regulators. The addition of BAP up to 0.3 mgL-1 increased the leaf number, which however decreased at higher BAP concentration.Keywords: BAP, black orchid, Coelogyne pandurata, multiplication, NAA ABSTRAKAnggrek hitam merupakan flora langka asli Kalimantan, Indonesia. Keberadaa anggrek ini di alam semakin langka akibat eksploitasi berlebihan dan sulitnya perbanyakan secara alami. Kultur jaringan merupakan metode untuk mengatasi kelangkaan anggrek ini. Penelitian ini bertujuan untuk mengetahui pengaruh kombinasi NAA dan BAP terhadap multiplikasi anggrek hitam. Media dasar yang digunakan adalah ½ MS dengan penambahan air kelapa 150 mLL-1. Eksplan yang digunakan adalah tunas hasil semai biji umur 6 bulan. Kultur tunas diinkubasi selama 23 minggu. Hasil penelitian menunjukkan bahwa kombinasi terbaik untuk multiplikasi tunas adalah NAA 0 mgL-1 dengan BAP 0,2 mgL-1. Tunas tumbuh lebih baik dalam media dengan penambahan BAP tanpa NAA, sedangkan akar pada media tanpa NAA dan BAP. Penambahan BAP sampai 0.3 mgL-1 mampu meningkatkan jumlah daun, namun menurun dengan penambahan di atas konsentrasi tersebut.Kata Kunci: anggrek hitam, BAP, Coelogyne pandurata, multiplikasi, NAA
Pembibitan (Kultur Jaringan hingga Pembesaran) Anggrek Phalaenopsis di Hasanudin Orchids, Jawa Timur Zahra Fadhlia Yasmin; Syarifah Iis Aisyah; Dewi Sukma
Buletin Agrohorti Vol. 6 No. 3 (2018): Buletin Agrohorti
Publisher : Departemen Agronomi dan Hortikultura

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (302.36 KB) | DOI: 10.29244/agrob.v6i3.21113

Abstract

Kegiatan penelitian di kebun Hasanudin Orchids bertujuan meningkatkan pengetahuan dan keterampilan mahasiswa dalam aspek budidaya anggrek Phalaenopsis. Tujuan secara khusus pada penelitian ini adalah mempelajari proses pembibitan anggrek Phalaenopsis dari kultur jaringan hingga pembesaranuntuk menghasilkan tanaman yang bernilai jual tinggi di Hasanudin Orchid. Hasil pengamatan menunjukkan pada pembuatan media kultur  PDA terjadi kontaminasi sebesar 7% dan media VW sebesar 22%. Proses penyemaian atau sub kultur, terutama pada sub kultur 1 banyak ditemukan kontaminasi. Hal ini terjadi karena beberapa faktor, diantaranya planlet yang luka pada saat pindah media. Persentase keberhasilan pada saat aklimatisasi adalah 100%. Pencegahan kehilangan akibat kontaminasi perlu diperhatikan secara intensif. Pembibitan mulai dari bibit muda, tanaman remaja, dan tanaman dewasa di dua green house tidak menunjukkan perbedaan signifikan. Perlu dilakukan beberapa pengembangan agar menghasilkan tanaman yang bernilai jual tinggi.
Improving Performance of Coleus through Mutation Induction by Gamma Ray Irradiation Aisyah, Syarifah Iis; Marthin, Yodi; Damanik, M. Rizal M.
Journal of Tropical Crop Science Vol 2 No 1 (2015): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (158.204 KB) | DOI: 10.29244/jtcs.2.1.26-32

Abstract

The objective of this study is to study the effect of gamma ray irradiation (15, 30, 45, and 60 gray (Gy) to determine Lethal Dose 50 (LD50) values, and to obtain new Coleus variances in a relatively short time. The study was conducted in a greenhouse at Cikabayan experimental field, Bogor Agricultural University, Darmaga, Bogor in May to July 2013. Gamma irradiation treatment significantly affected height, number of leaves, and number of nodes. Increasing the dose level of gamma irradiation tend to inhibit plant growth. LD50 for yellow/green, green/brown, variegated green/brown of Coleus blumei, and Coleus amboinicus Lour were 48.66, 65.2, 52.81, and 37.62 Gy respectively. C. amboinicus  irradiated at a dose level of 45 Gy had different leaf shapes compared to control. Keywords:  Coleus, gamma ray irradiation, LD50 values, ornamental plant, torbangun