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All Journal Mutiara Medika: Jurnal Kedokteran dan Kesehatan INDONESIAN JOURNAL OF PHARMACY Berkala Kedokteran Jurnal Abdimas Mahakam Bubungan Tinggi: Jurnal Pengabdian Masyarakat Prosiding Seminar Nasional Program Pengabdian Masyarakat Jurnal Farmasi Sains dan Praktis
Sri Tasminatun
Bagian Farmakologi, Fakultas Kedokteran Dan Ilmu Kesehatan, Universitas Muhammadiyah Yogyakarta
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Economics, Econometrics & Finance Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Public Health Social Sciences Other

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Chemopreventive effect of ethanolic extract of Gynura procumbens (Lour), Merr on the carcinogenesis of Rat breast cancer development Meiyanto, Edy; Susilowati, Sri; Tasminatun, Sri; Murwanti, Retno; ., Sugiyanto
INDONESIAN JOURNAL OF PHARMACY Vol 18 No 3, 2007
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (248.982 KB) | DOI: 10.14499/indonesianjpharm0iss0pp154-161

Abstract

Gynura procumbens (Lour) Merr., empirically, used to prevent cancer development and has been proven to be able to suppress lung cancer development. The aim of this research is to examine the potential of ethanolic extract of G. procumbens to suppress DMBA-induced breast cancer development. Sprague Dawly Rats were used in this research and were grouped as indicated treatment. Ethanolic extract of G. procumbens was administered into 3 levels of doses, namely 250, 500, and 750 mg/kgBW. Tumor development was examined by palpation every week and terminated at week 16th after the end of DMBA treatment. The result showed that extract treatment at the dose of 250, 500, and 750 mg/kgBW reduced tumor incidence by 60%, 30 %, and 20 % respectively. The doses of 500 and 750 mg/kgBW exhibited strong suppression of tumor multiplicity, where as the dose of 250 performed less potential suppression. In conclusion, ethanolic extract of G. procumbens performs chemopreventive effect to suppress breast cancer development at the dose of 250 mg/kgBW.Key words : chemopreventive, Gynura procumbens (Lour) Merr, breast cancer.
POTENTIAL ETHANOL OF Carica papaya L. EXTRACT AS IMMUNOMODULATORY THROUGH HISTOLOGY OBSERVATION AT MICE BALB/C SPLEEN Tasminatun, Sri; Pravitasari, Ria; Makiyah, SN. Nurul
Berkala Kedokteran Vol 13, No 2 (2017)
Publisher : Fakultas Kedokteran Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/jbk.v13i2.4077

Abstract

Abstract: The diameter of the alba pulp of the spleen will be enlarged, when the body responds to allergens by increasing the activity of the immune system. Papaya fruits (Carica papaya L.) containing flavonoid that can be as an immunomodulator. This study aims to determine the effect of C. papaya extract to the diameter size alba pulp of the spleen of Ovalbumin (OVA) induced Balb/C mice. The research design was experimental in vivo animal test with post-test only control group design. Thirty male Balb/C mice, ±20grams weight, 8 weeks old, divided into six groups consists of a normal control, negative control, Methylprednisolone, and C. papaya extract treatment group (175mg/kg, 350mg/kg and 700mg/kg dose per day). The extract and Methylprednisolone are given for 28 days, while OVA is given on 15thand 22ndday (intraperitoneal,) and 23rd until 28thday (peroral). On the 29thday the mice are sacrificed and the duodenum was made into histological preparation with HE staining. The immunomodulator effect is assessed by the average of the diameter size alba pulp of the spleen under a light microscope. The data were analyzed using SPSS15 with one way ANOVA continued with Tukey test. The average of the diameter size alba pulp of the spleen for control group is 10,165 ± 0,195; negative control group is 30,357 ± 0,826; methylprednisolone group is 10,220 ± 0,346; 175mg/kgbb dose extract is 14,956 ± 1,057; 350mg/kgbb dose extract is 14,030 ± 0,667; and 700mg/kgbb dose extract is 12,504 ± 0,729. The conclusion of this study is Carica papaya L. extract treatment group (175mg/kg, 350mg/kg and 700mg/kg dose per day) has an effect to the average of the diameter size alba pulp of the spleen in OVA-induced Balb/C mice significantly. 700mg/kg dose per day has the same effect with the methylprednisolone group. Keywords: immunomodulator, Carica papaya L., alba pulp of the spleen, ovalbumin
Ekspresi Cyclooxygenase-2 dan angiogenesis pada kanker colorectal: Penelitian di Wuhan University, China Tasminatun, Sri
Mutiara Medika: Jurnal Kedokteran dan Kesehatan Vol 5, No 1 (2005)
Publisher : Universitas Muhammadiyah Yogyakarta

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Abstract

Cyclooxygenase-2 (COX-2) penting pada berbagai fungsi seluler antara lain pertumbuhan dan deferensiasi sel, invasi sel kanker, angiogenesis dan fungsi imun. Angiogenesis sangat berperan pada perkembangan sel-sel kanker. Hubungan antara COX-2 sebagai faktor angiogenik pada kanker colorectal belum diketahui dengan jelas.Tujuan:Penelitian ini bertujuan untuk menganalisis hubungan antara ekspresi COX-2 dalam kanker colorectal dengan vascular endothelial growth factor (VEGF) dan microvascular density (MVD).Metode :Ekspresi cyclooxygenase-2 ,VEGF dan MDV dianalisa dengan tehnikimunohistokimia pada 128 kasus kanker colorectal. Selanjutnya dilakukan analisis hubungan antara ekspresi COX-2 , ekspresi VEGF dan MVD.
Uji Toksisitas Subkronis Ekstrak Etanolik Biji Srikaya (Annona squamosa L.) Sebagai Repelan Makiyah, Sri Nabawiyati Nurul; Tasminatun, Sri
Mutiara Medika: Jurnal Kedokteran dan Kesehatan Vol 6, No 1 (2006)
Publisher : Universitas Muhammadiyah Yogyakarta

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Abstract

Active compound in A. squamosa seed such as carvon and limonene has a toxic effect on skin cell and can be absorbed into bloodflow, so has a systemic effect on liver, lung and kidney. The aim of this research is to study the toxic effect of ethanolic extract of A. squamosa seed as repellant subchronically.Thirty rats (Rattus norvegicus) Wistar strain, male and female, 3 month old used as subject. It is divided into 5 groups, negative control groups, positive control group with CMC, and three group of treatment group used ethanolic extract of A. squamosa seed dosage I (25%), II (50%) dan III (100%). The back hair of the rats were showed with its diameter 1,5cm of it and given a treatment topically for each groups every day for thirty days and observed its condition if any change happens every days. On day 31, all of the rats decapitated, a skin located of treatment is taken and so its liver, lung and kidney to make a histopathological preparation with H&E.The result of this research showed that subchronic ethanolic extract of A. squamosa seed 25%, 50% and 100% topically caused irritation on skin, but not caused of change in histological features of liver, lung and kidney. Subchronic ethanolic extract of A. squamosa seed 100% significantly caused irritation and increased of polimorphonuclear cell.Senyawa aktif dalam biji antara lain carvon dan limonene mempunyai efek toksik pada sel- sel kulit dan dapat terabsorpsi masuk ke dalam aliran darah, sehingga memberikan efek sistemik pada organ hepar, pulmo dan ginjal. Penelitian ini bertujuan untuk mengetahui efek toksik ekstrak etanolik biji A. squamosa sebagai repelan yang digunakan secara subkronis.Subyek penelitian adalah tikus putih (Rattus norvegicus) galur Wistar jantan dan betina, umur3 bulan, 30ekor. Subyekdibagimenjadi5kelompokyaitukelompokkontrolnegatif,kelompok kontrol CMC, kelompok ekstrak etanolik biji A. squamosa konsentrasi I (25%), II (50%) dan III (100 %). Punggung tikus dicukur dengan diameter 1,5 cm, diberi perlakuan pada hari kel-30 sesuai kelompoknya. Ekstrak dioleskan setiap hari di tempat yang dicukur. Kondisi umum tikus diamati dan dicatat perubahan yang teijadi setiap hari. Pada hari ke-31, semua tikus dikorbankan, kulit tempat perlakuan, hepar, paru dan ginjal diambil dan dibuat preparat histologi dengan pengecatan H&E.Hasil penelitian menunjukkan pemberian subkronis ekstrak etanolik biji srikaya (Annona squamosa) 25%, 50%, 100% secara topikal menyebabkan iritasi pada kulit, tetapi tidak menyebabkan perubahan histologis organ hepar, paru dan ginjal, ekstrak etanolik A. squamosa 100% topikal subkronis secara signifikan dapat menyebabkan iritasi dan meningkatnya jumlah polimorfonuklear sel.
Efektivitas Krim Ekstrak Zingiber officinale Linn. var. rubrum sebagai Penurun Nyeri Sendi pada Lansia Setyawan, Ricky Andy; Tasminatun, Sri
Mutiara Medika: Jurnal Kedokteran dan Kesehatan Vol 13, No 2 (2013)
Publisher : Universitas Muhammadiyah Yogyakarta

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Abstract

Lanjut usia (Lansia) adalah kelompok khusus yang memiliki banyak masalah kesehatan salah satunya adalah nyeri. Jahe merah (Zingiber officinale Linn. var. rubrum)  berguna sebagai obat modern maupun tradisional. Penelitian ini bertujuan untuk mengetahui efektivitas krim Z. officinale Linn. var. rubrum terhadap intensitas nyeri sendi pada lansia. Penelitian ini adalah eksperimental dengan pre-test & post-test control group design. Subjek penelitian adalah 36 lansia yang menderita nyeri sendi dan tidak sedang mengkonsumsi atau menggunakan obat analgetik atau OAINS. Subjek dibagi dalam 3 kelompok. Kelompok krim ekstrak Z. officinale 10%, 20%, dan 0%. Penurunan intensitas nyeri sendi diukur dengan metode Visual Analogue Scale (VAS) selama 30 menit. Hasil penelitian menunjukkan penurunan nilai VAS pada ketiga kelompok. Pada kelompok ekstrak krim Z. officinale 10% rata-rata terjadi penurunan sebesar 4.75, kelompok ekstrak krim Z. officinale 20% sebesar 3.08 dan 1 pada kelompok basis krim. Hasil perbandingan analisis data pada kelompok Z. officinale 10% dan 20% menunjukkan perbedaan yang bermakna (p < 0.05) dengan Paired T test dan Z.officinale 10% lebih efektif daripada Z. officinale 20% dengan Kruskal-Wallis test yang masing-masing kelompok berbeda bermakna (p < 0.05) pada Mann-Whitney test. Disimpulkan bahwa krim ekstrak jahe merah (Z. officinale. Linn. var. rubrum) dengan konsentrasi 10% dan 20% terbukti bermakna dapat menurunkan intensitas nyeri sendi pada lansia. Konsentrasi krim ekstrak Z. officinale. Linn. var. rubrum 10% pada penelitian ini lebih efektif dari pada konsentrasi 20%. Elderly is a group with many health problems and one of those is pain. Ginger used as modern or traditional medicine. This research aims to determine the effectiveness of Z. officinale Linn. var. rubrum cream on the intensity of joint pain in the elderly. This research is experimental with pre-test and post-test control group design. Subject is 36 elderly people with joint pain and does not having any analgetic agents or NSAID. Subject divided into 3 group. Group of ginger extract cream 10%, 20% and 0%. Decreasing intensity of pain measured by Visual Analgue Scale method during 30 minutes. The study result shows decreasing of VAS score in all groups. There are 4.75; 1,308 & score value in group 1, 2, 3 respectively. The results of comparative analysis of the data in study of group 1 and 2 showed significant differences (p < 0.05) with Kruskal-Wallis test which each group is significantly different with MannWhitney test (p < 0.05). The conclusion is the Z. officinale. Linn. var. rubrum extract cream with concentration of 10% and 20% has been proven meaningful to reduce the joint paint intensity on elderly. The 10% concentration of Z. officinale. Linn. var. rubrum extract cream is more effective  than the 20%
Efek Infusa Batang Brotowali (Tinospora crispa) terhadap Nafsu Makan Dan Berat Badan Tikus Putih (Rattus norvegicus) Tasminatun, Sri; Wahyuningsih, Nur
Mutiara Medika: Jurnal Kedokteran dan Kesehatan Vol 7, No 2 (s) (2007): Oktober
Publisher : Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/mmjkk.v7i2 (s).1663

Abstract

T. crispa is a herbal medicine that proved as a drug for patient Diabetes mellitus. But, our community also use it to increase the appetite and body weight. The aim of this research was to find out the appetite and body weight effect of T. crispa‘s stem infusion in female white mouse. The subject are 25 female mouse, 2 month years old and 110 gram of body weight. They are divided into 5 groups, 3 groups of treatment with different dose (1,28 g/kgBW, 2,56 g/kgBW, and5,12 g/kgBW) and 2 groups control.. Everyday they will injection with infusion of T. crispa and every 5 days their body weight will be measured. It lasted for 20 days. The data will be analyzed by Anova one way, continued Tukey test with help of computer program of SPSS (p 0,05) According to this research, it could be knew that T. crispa‘s stem infusion could increase appetite and body weight. The increasing of appetite was seen at dose 5,12 g/kgBW at 10 days after get infusion. The increasing of body weight was seen at the first dose (1,28 g/ kgBW) for 10 days after get infusion.T. crispa merupakan herbal medicine yang telah terbukti dapat menunkan kadar gula darah pada penderita Diabetes mellitus. Namun, T. crispa ini juga sering digunakan oleh masyarakat sebagai ramuan yang dapat meningkatkan nafsu makan dan berat badan. Masyarakat biasanya menggunakan batang tanaman ini untuk ditumbuk kemudian di buat ramuan dan diminum sehari- hari. Penelitian ini bertujuan untuk mengetahui efek T. crispa sebagai penambah nafsu makan dan berat badan. Penelitian ini dilakukan pada 25 ekor tikus putih betina (Rattus norvegicus) galur SD berumur 2 bulan dengan berat ±110 gram. Subyek dibagi menjadi 3 kelompok perlakuan dan 2 kelompok kontrol. Pada kelompok perlakuan tiap hari tikus di sonde dengan infusa T .crispa sesuai dengan dosisnya (1,28 g/kgBB, 2,56 g/kgBB, dan 5,12 g/kgBB). Setelah 5 hari penelitian tiap hewan uji ditimbang dan dirata-rata berat badannya selama 20 hari. Data yang diperoleh kemudian di analisa dengan uji Anova satu jalan dilanjutkan uji Tukey dengan bantuan program komputer SPSS (p 0,05). Berdasarkan hasil penelitian didapatkan bahwa infusa batang T. crispa dapat meningkatkan nafsu makan dan berat badan. Peningkatan nafsu makan terjadi pada dosis 5,12 g/kgBB selama 10 hari pertama pemberian infusa, setelah itu nafsu makan tidak meningkat lagi. Peningkatan berat badan diperoleh pada dosis 1.28 g/kgBB selama 10 hari pertama pemberian infusa, setelah itu berat badan tidak meningkat lagi.
Pengaruh Virgin Coconut Oil terhadap Kadar Kolesterol, HDL dan LDL Tikus Putih (Rattus norvegicus) Yudha, Raditya Purna; Tasminatun, Sri
Mutiara Medika: Jurnal Kedokteran dan Kesehatan Vol 8, No 1 (s) (2008): April
Publisher : Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/mmjkk.v8i1 (s).1641

Abstract

Recently, cardiovascular disease becomes an obstacle to do a daily activities. VCO is one of the solutions to decrease cholesterol blood score.The aim of this research is to know the influence of VCO (Virgin Coconut Oil) toward cholesterol, LDL and HDL score on Rattus norvegicus. The research design used an experimental laboratory in vivo on animah test which used Rattus norvegicus, Spraque Dawley strain, 2 months age, 130-215g body weight as subject. Twenty rats were divided into 4 groups. Three groups were VCO ’s treatment and 1 group was a control group. Those 3 groups were given three different VCO ’s brand each others orally everyday during 30 days and the formula doses were converted with human dose standard. Then on 31st day, all rats are killed to check the blood score with micro capillary pipe. The blood score were measured by Spectrophotometer and analyzed by ANOVA one way, continued with Tukey Test. Cholesterol and LDL score which given VCO treatment, its score higher than control group. HDL score on the group which given VCO lower than control group. There is not any significant (P 0.05)between the influence of VCO toward Rattus norvegicus’ cholesterol, LDL and HDL score.Akhir-akhir ini penyakit kardiovaskuler dapat mengganggu aktifitas kerja, sehingga manusia mencari jalan keluar untuk mengatasinya. Salah satunya dengan cara menggunakan VCO yang dapat menurunkan kadar kolesterol darah. Tujuan penelitian ini adalah untuk mengetahui pengaruh VCO (Virgin Coconut Oil) yang berada di pasaran terhadap kadar kolesterol, LDL, dan HDL tikus putih (Rattus norvegicus). Desain penelitian ini adalah penelitian eksperimental in vivo pada hewan uji dengan tikus putih (Rattusnorvegicus) galur SD jantan umur 2 bulan, berat badan 130-215g. Duapuluh ekor tikus putih dibagi menjadi 4 kelompok yaitu: 3 kelompok perlakuan dan 1 kelompok kontrol. Kelompok perlakuan diberi VCO dengan 3 merek berbeda per oral setiap hari selama 30 hari dengan dosis yang dikonversikan dari dosis pada manusia. Pada hari ke 31 tikus dikorbankan, diperiksa darah menggunakan pipa kapiler. Hasil kadar kolesterol diukur menggunakan Spektrofotometri, dan dianalisis menggunakan uji Anova satu jalan dilanjutkan uji Tukey. Hasil penelitian menunjukkan bahwa kadar kolesterol dan LDL kelompok perlakuan yang diberi VCO lebih tinggi daripada kelompok kontrol dan secara statistik tidak berbeda secara bermakna (p 0,05). KadarHDL kelompok yang diberi VCO lebih rendah daripada kelompok kontrol dan secara statistik juga tidak signifikan (p 0,05).
Pengaruh Kitosan secara Topikal terhadap Penyembuhan Luka Bakar Kimiawi pada Kulit Rattus norvegicus Pramono, Barii Hafidh; Husein, Rizqi Afrian Jamaludin; Tasminatun, Sri; Wardono, Aditiya Pramudya
Mutiara Medika: Jurnal Kedokteran dan Kesehatan Vol 12, No 3 (2012)
Publisher : Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/mmjkk.v12i3.1046

Abstract

Kontak antara kulit dengan zat kimia iritatif seperti asam sulfat dapat menyebabkan luka bakar kimiawi. Senyawa yang telah diteliti efektif mempunyai kemampuan mengakselerasi proliferasi sel, migrasi sel PMN, daya antiinfeksi, dan bersifat basa adalah kitosan. Penelitian ini bertujuan untuk menganalisis pengaruh kitosan secara topikal terhadap penyembuhan luka bakar kimiawi pada kulit tikus putih terinduksi asam sulfat. Penelitian eksperimental invivo, sebanyak 30 ekor tikus dibagi enam kelompok (kontrol tanpa perlakuan, kontrol vaselin, kontrol Bioplacenton®, salep kitosan dosis 1,25%, 2,5%, dan 5%). Luka bakar kimiawi diinduksi dengan 0,1 ml asam sulfat 75%. Data dianalisis dengan metode ANAVA dilanjutkan uji Tuckey. Waktu sembuh paling cepat adalah kelompok salep kitosan dosis 2,5% (20,2±2 ,9 hari) dan yang paling lama adalah kelompok kontrol tanpa perlakuan (30,4±4 ,8 hari). Pemberian salep kitosan dosis 1,25%, 2,5%, dan 5% signifikan mempercepat waktu sembuh dibandingkan kontrol tanpa perlakuan dan vaselin. Salep kitosan 2,5% paling signifikan mempercepat waktu sembuh dibandingkan kontrol tanpa perlakuan dan vaselin (p=0,003; p=0,006). Persentase penyembuhan kelompok salep kitosan 1 ,25%, 2,5%, dan 5% mempunyai kurva peningkatan persentase lebih tinggi dari semua kelompok kontrol. Salep kitosan 2,5% signifikan meningkatkan persentase penyembuhan dibandingkan semua kelompok kontrol termasuk kontrol positif® ( p=0,  Contact between skin with chemicals substance such as sulfuric acid can cause chemical burns. A compound that had been researched effective have ability to accelerate cell proliferation, migration of PMN cells, the anti-infective, and the base pH is Chitosan. The aims is to determine the effect of topical chitosan on chemical burn healing in skin rat induced sulfuric acid. Experimental in-vivo with  30 female rats divided into six groups (control without treatment, Vaseline control, Bioplacenton® control, Chitosan ointment 1.25%, 2.5%, and 5% dose). Chemical burn was induced with 0.1 ml of 75% sulfuric. Data were analyzed by Anova method continued with Tuckey test. The results of healing time which fastest was Chitosan ointment 2.5% (20.2±2.9 days) and which longest was control without treatment group (30.4±4.8 days). Chitosan ointment 1.25%, 2.5%, and 5% dose significantly accelerated healing time compared with without treatment and Vaseline control groups. Chitosan ointment 2.5% most significantly accelerated the healing time compared with without treatment and Vaseline control (p=0.003 p=0.006). The healing percentage of Chitosan ointment 1.25%, 2.5%, and 5% dose have higher percentage increasing curve than all control groups. Chitosan ointment 2.5% dose significantly increased the healing percentage than all control groups including positive control (p=0.008). It was concluded that chitosan have an influence on the healing of chemical burn. 
Efektivitas Salep Kitosan terhadap Penyembuhan Luka Bakar Kimia pada Rattus norvegicus Putri, Fitri Rizkia; Tasminatun, Sri
Mutiara Medika: Jurnal Kedokteran dan Kesehatan Vol 12, No 1 (2012)
Publisher : Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/mmjkk.v12i1.997

Abstract

Proses penyembuhan luka adalah satu respon terkoordinasi pada cedera jaringan yang menghasilkan kontraksi jaringan, penutupan luka, dan pemulihan. Penelitian sebelumnya telah menunjukkan bahwa salep kitosan kadar 2,5% efektif dalam mempercepat penyembuhan luka dan meningkatkan persentase penyembuhan. Penelitian ini bertujuan untuk mengetahui efek kitosan terhadap gambaran histologis penyembuhan luka bakar kimia pada kulit melaui pengamatan ketebalan epitel dan jumlah fibroblas. Studi in vivo, tikus Sprague Dawley dibagi menjadi enam kelompok, kontrol tanpa perlakuan, kontrol vaselin, kontrol Bioplacenton®, salep kitosan dosis 1,25%, 2,5%, dan 5%. Punggung tikus diinduksi luka bakar derajat tiga dengan asam sulfat 75%. Formula kitosan dioleskan tiap hari sampai kriteria sembuh terkonfirmasi. Jaringan kulit yang telah sembuh dibuat preparat histologi dengan pewarnaan HE kemudian diukur ketebalan epitel dan jumlah fibroblasnya. Data dianalisis dengan ANOVA satu arah, dilanjutkan dengan LSD. Hasil penelitian menunjukkan bahwa ketebalan epitel paling tipis dihasilkan oleh salep kitosan 5% (13,31 ± 4,05) dengan nilai signifikansi (p = 0,015). Jumlah fibroblas yang paling sedikit dihasilkan oleh salep kitosan 5% (49, 80 ± 6,01 sel) dengan nilai signifikansi (p = 0,000). Penelitian ini membuktikan bahwa dosis salep kitosan terbaik adalah 5%. The process of wound healing is a set of coordinated responses to tissue injury that results in tissue contraction, closure, and restoration. Previous study had shown that chitosan ointment 2,5% is effective in promoting wound healing and increasing healing percentage. This experiment has aim to gain the chitosan effect on histological properties in chemical burn healing of skin through epithelial thickness and number of fibroblast observation. In in vivo studies, rats Sprague Dawley were divided into six groups; control without treatment, vaseline control, Bioplacenton® control, chitosan ointment dose 1.25 %, 2.5%, and 5%. A third degree burn of the backskin was performed by 75% sulfate acid. Chitosan formulations were day repeatedly applied on the burned areas until the final healing process criterias were confirmed. Healing tissue was evaluated by histology preparation with HE staining then its epithelial thickness and number of fibroblast were measured. The datas were analyzed by one way ANOVA, followed by LSD. The results indicated that the thinnest epithelial thickness was showed on chitosan 5 % (13.31 ± 4.05) with a value of significance (p=0,015). The fewest number of fibroblast is performed on chitosan 5% (49, 80 ± 6.01 cells) with a value of significance (p = 0,000). This study proved the best chitosan ointment dose is 5%.
Efek Pemberian Madu sebagai Profilaksis pada Ulkus Lambung Tikus Putih (Rattus norvegicus) Diinduksi Aspirin Kusumawati, Wiwik; Tasminatun, Sri
Mutiara Medika: Jurnal Kedokteran dan Kesehatan Vol 8, No 1 (2008)
Publisher : Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/mmjkk.v8i1.1651

Abstract

Honey is widely used to cure many disorders including digesting system disorder, but the scientifical evidence remains lacking. One of the most recurring digesting system disorders is gastric ulcer. This research is aimed therefore at figuring out the effect of honey on aspirin induced gastric ulcer on rat. The subject consist of 30 rats Wistar strain, classified into 5 groups, 2 control such as positive control group exposed to cimetidine and negative control group exposed to amylum and 3 treatment groups. Treatment group is treated with honey in 3 levels of dosages, which are 30 ml/day, 45 ml/day and 60 ml/day given orally for 2 days then left for 48 hours without meal. Aspirin 150 mg/kgBWis given as ulcer induction. All the subjects are then decapitated. The gasters are later examined and the ulcers appearing on the gaster are calculated using modified Ralph method. The observation result is then analyzed and statistically tested using Kruskal Wallis and Man Whitney Test, with reliability level of 95%. The result shows that the score of group exposed to amylum (9,08 ± 4,43) is significantly different from group exposed to cimetidin (1,42 ±1,11) but is not significantly different from group exposed to honey treatment, sequenced from the smallest dosage to the highest (3,75 ± 4,78; 6,50 ± 4,43; 4,58 ± 2,94). Meanwhile, the group exposed to honey with 30 ml/day dosage shows ulcer score of 3,75± 4,78 which is slightly different from that group exposed to cimetidin 1,42 ±1,11.Madu banyak digunakan untuk mengatasi berbagai gangguan atau penyakit termasuk gangguan pada saluran pencernaan. Salah satu gangguan pencernaan yang bersifat kronis dan kumat-kumatan antara lain adalah ulkus lambung. Penelitian ini dilakukan untuk mengetahui efek profilaksis madu pada ulkus lambung tikus yang ditimbulkan olah aspirin. Penelitian dilakukan pada 30 ekor tikus putih jantan galur Wistar yang dibagi menjadi 5 kelompok terdiri dari 2 kelompok kontrol dan 3 kelompok perlakuan. Kelompok kontrol terdiri dari kelompok kontrol positif diberikan cimetidin dan kontrol negatif diberikan amilum. Kelompok perlakuan diberikan madu dalam 3 peringkat dosis yaitu 30 ml/hari, 45 ml/hari dan 60 ml/hari. Bahan uji diberikan selama 2 hari secara oral, selanjutnya dipuasakan selama 48 jam. Ulkus lambung diinduksi dengan pemberian aspirin 150 mg/kgBB. Hewan uji didekapitasi dan lambung diperiksa, dihitung jumlah ulkus dan perdarahan menurut cara Ralph yang dimodifikasi. Hasil pengamatan dianalisis dengan uji statistik nonparametrik Kruskal Wallis dengan tarafkepercayaan 95 % dilanjutkan dengan uji Man Whitney Test. Hasil penelitian menunjukkan perbedaan skor ulkus yang bermakna antar kelompok. Didapatkan skor ulkus kelompok amilum (9,08 ± 4,43) berbeda bermakna dengan kelompok cimetidin (1,42 ±1,11) tetapi tidak berbeda bermakna dengan kelompok madu sesuai urutan dosis terkecil (3,75 ± 4,78 ; 6,50 ± 4,43 ; 4,58 ± 2,94) namun demikian kelompok madu dosis 30 ml/hari menunjukkan skor ulkus 3,75± 4,78 tidak berbeda signifikan dengan cimetidin 1,42 ±1,11.
Co-Authors Arum Indrasari, Arum Asifa, Nadia Nur Barii Hafidh Pramono, Barii Hafidh Edy Meiyanto Edy Meiyanto Eni Istiyanti Erika Loniza Fitri Rizkia Putri Fitri Rizkia Putri, Fitri Rizkia Kurnia Chairunnisa Nadia Nur Asifa Noerma Shovie Rizqiea Nugraheni, Peni Nur Shani Meida Nur Wahyuningsih NUR WAHYUNINGSIH Pravitasari, Ria Raditya Purna Yudha Retno Murwanti Ria Pravitasari Ricky Andy Setyawan Rizqi Afrian Jamaludin Husein, Rizqi Afrian Jamaludin Safinta Nurindra Rahmadhia Sarjiyah Sarjiyah, Sarjiyah Setyawan, Ricky Andy Sherly Usman Siti Aminah Tri Susila Estri SN Nurul Makiyah SN. Nurul Makiyah Sri Nabawiyati Nurul Makiyah Sri Susilowati Sri Susilowati Susilowati Sugiyanto . Sugiyanto . Wardono, Aditiya Pramudya Wiwik Kusumawati, Wiwik Yudha, Raditya Purna Yuli Kuswandari