Garuda - Garba Rujukan Digital

Article Per Year (5 Year)

p-Index From 2019 - 2024

P-Index

This Author published in this journals

All Journal Medical Journal of Indonesia BERITA BIOLOGI YARSI Medical Journal

MIRNAWATI SUDARWANTO

Fakultas Kedokteran Hewan, Institut Pertanian Bogor, Dramaga, Bogor

Author-ID : 387826

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Medicine & Pharmacology

Published : 4 Documents Claim Missing Document

Claim Missing Document

Articles

Using homeopathica drugs combination at peripartal phase in preventing bovine subclinical mastitis Sanjaya, Agatha W.; Wibawan, I W.T.; Sudarwanto, Mirnawati; Widodo, Setyo; Enbergs, Heinrich
Medical Journal of Indonesia Vol 13, No 4 (2004): October-December
Publisher : Faculty of Medicine Universitas Indonesia

A total of 33 dairy lactating cows suffering from subclinical mastitis were classified into group A (10 cows), group B (12 cows) and group C (11 cows). They were treated with the combination of homeopathic drugs and placebo, applied at the 4th and 3rd week antepartum (a.p), and continuing every week postpartum (p.p), for four times. Group A received Coenzyme compÂ® (in the 4th and 3rd week a.p), Lachesis compÂ® combined with TraumeelÂ®(1st and 2nd week p.p) and Coenzyme compÂ® combined with Carduus compÂ® (3rd and 4th week p.p). Group B received TraumeelÂ®+ Mucosa compÂ® (4th and 3rd week a.p), Lachesis compÂ®+ TraumeelÂ® (1st and 2nd week p.p) and Coenzyme compÂ®+ Carduus compÂ® (3rd and 4th week p.p) and group C as a placebo. The incidence of subclinical mastitis in group A and C appeared irregular. In contrast, group B showed a constant percentage (33.3%). Group A and B showed significant response to the homeopathic drugs, expressed as an increasing of the somatic cell count value. At peripartal phase, haptoglobin increased in group B 0.80 mg/ml and group A as well as placebo 1.40 mg/ml. After calving, group B expressed a constant value (0.05 mg/ml), while group A and placebo rose significantly. The milk yield in normal lactation period (the 3rd - 7th month) increased significantly, with an increasing 14.1% for group B and 4.9% A respectively. (Med J Indones 2004; 13: 221-6)Keywords: Homeopathy, Homeopathic combination, Subclinical Mastitis, Peripartal Phase

Detection of Mycobacterium avium subspecies paratuberculosis in formula milk from Bogor using PCR IS 900 Nugroho, Widagdo S.; Sudarwanto, Mirnawati; Lukman, Denny W.; Naim, Rochman; Hassan, Abdulwahed A.; Usleber, Ewald
Medical Journal of Indonesia Vol 17, No 3 (2008): July-September
Publisher : Faculty of Medicine Universitas Indonesia

Crohnâs disease (CD) that becomes a public health concern in developed countries shows similarities in clinical signs and pathological features with Johneâs disease (JD) in ruminants infected by Mycobacterium avium subspecies paratuberculosis (MAP). Few researches conducted in Europe, the USA, and Australia showed relationships between MAP, CD, JD and dairy products. Indonesians consume milk and diary products from domestic and imported source. Adji in 2004 found some domestic dairy cows that were seropositive for MAP, and this could be a serious problem in dairy farm animals and human health in the future. The aim of this study was to detect MAP in the growing up formula milk. Fifty samples from five established factories were taken from supermarkets in Bogor. Polymerase chain reaction method (PCR) with insertion sequence (IS) 900 as primer and culture in Herroldâs egg yolk media with mycobactin J (HEYM J) as a gold standard were used in this study. Neither MAP grew up in HEYM J medium after 20 weeks of culture period nor positive samples by PCR IS 900 were found. Although there were no positive samples found in this study, further extensive and comprehensive studies on MAP should be done with more and varied samples, as well as in human to provide data on MAP in Indonesia. (Med J Indones 2008; 17: 183-7)Keywords: Crohnâs disease, dairy cow, growing up formula milk

GEJALA KLINIS DAN PATOLOGI ANATOMI PASCA INFEKSI Campylobacter jejuni PADA AYAM BROILER [Clinical Symptoms and Pathology Anatomy due to Infection Campylobacter jejuni on Broilers] Andriani, Andriani; Sudarwanto, Mirnawati; Setiyaningsih, Surachmi; Kusumaningrum, Harsi Dewantari; Pisestyani, Herwin
BERITA BIOLOGI Vol 11, No 3 (2012)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Campylobacter spp. is a bacteria foodborne zoonotic agent that can infect humans and animals, especially birds. This bacteria can cause important problems in the public health since the beginning at the farm level, the level of retail and on the table. Poultry meat is the main source of contamination because the digestive tract of poultry is the predilection of Campylobacter jejuni. During this infection C. jejuni in poultry did not show typical clinical symptoms, making detection quite difficult to diagnose this disease at the farm level. Incidence infection of Campylobacter sp. in developed countries had 5-90%. Currently poultry production and consumption are predicted to still increase due to a high demand for low price protein. This research is conducted to determine the clinical and pathologic anatomy due to an infection of C. jejuni on chicken digestive tract. A total of 105-day-old chickens are divided into 3 groups, each consisting of 35 individuals. Group A was the control group (not infected) group B (infected with C. jejuni ATCC 33291), and group C (infected with field isolate of C. jejuni/C1). Infection of the chicken is done 0.5 ml peroral suspension concentration of104cfu/ml. Anova Duncan is used to analyze parameters of body weight, increase weight gain, feed consumption, FCR values. Kurskall Walis method used to analyze the parameters non-parametric form of the score changes in the macroscopic and microscopic intestinal and liver. Infection of C. jejuni changes the pathology anatomy of intestine i.e. hyperemia, enteritis and enteritis catharalis haemorrhagic. A microscopic change of intestinal is oedema,haemorrhage and infiltration of inflammatory cell. Changes of pathology anatomy liver are colour smeary, congesti, pale, fragile and necrotic. Microscopic changes of liver are degeneration and focal hepatic necrosis. Group observation infected by C. jejuni caused chicken cannot reach maximum body weight and can be detrimental to farmers because of increase of the value of the feed conversion ratio (FCR).

BIOSINTESIS ANTIGEN PERMUKAAN HEPATITIS B â€œHBSAG100â€ PADA ESCHERICHIA COLI DALAM RANGKA PRODUKSI PROTEIN REKOMBINAN SEBAGAI MODEL IMUNOGEN UNTUK MENGHASILKAN ANTIBODI Riyadi, Slamet; RA Maheswari, Rarah; Sudarwanto, Mirnawati; RZ, Fransiska; Ali, Muhamad
Jurnal Kedokteran YARSI Vol 18, No 2 (2010): MEI - AGUSTUS 2010
Publisher : Lembaga Penelitian Universitas YARSI

Biosintesis protein rekombinan melalui Escherichia coli memberikan alternatif untuk menghasilkan protein antigen yang bermanfaan bagi kepentingan kesehatan yang bebas dari protein manusia. Penelitian ini menggabungkan fragmen DNA dari antigen permukaan virus Hepatitis B dengan gen penyandi enzim gluthation-S-transferase (GST) di dalam plasmid p GEX-4T-2 yang di ekspresikan di dalam sel-sel Escherichia coli. Polypeptida dengan berat molekul sekitar 34,8 kDa telah diproduksi dan diidentifikasi sebagai protein gabungan GST-HB100. Protein gabungan tersebut kemudian dimurnikan menggunakan kolum GSTrap yang disambung dengan kolum HiTrap. Selanjutnya, protein hasil pemurnian tersebut diharapkan bisa digunakan sebagai bahan vaksin atau untuk menghasilkan antibodi.

Co-Authors Abdulwahed A. Hassan Agatha W. Sanjaya Andriani Andriani Denny W. Lukman Ewald Usleber Fransiska RZ Heinrich Enbergs Herwin Pisestyani I W.T. Wibawan Muhamad Ali RA Maheswari, Rarah Rochman Naim Setyo Widodo Slamet Riyadi Surachmi Setiyaningsih Widagdo S. Nugroho

Title

Found 4 Documents
Search

Abstract

Abstract

Abstract

Abstract

Title Search

Found 4 Documents Search

Abstract

Abstract

Abstract

Abstract

Title

Found 4 Documents
Search