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Toxic compounds of Curcuma aeruginosa causes necrosis of mice hepatocytes Eka Pramyrtha Hestianah; Idha Kusumawati; Lucia Tri Suwanti; Sri Subekti
Universa Medicina Vol. 33 No. 2 (2014)
Publisher : Faculty of Medicine, Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18051/UnivMed.2014.v33.118-125

Abstract

BACKGROUNDPeople have been using Curcuma aeruginosa rhizome as a traditional herbal medicine as appetite stimulant, without realizing its side effects. Herbal plants contain tens to hundreds of compounds, some of which are toxic. The aim of this research was to determine which toxic compound of Curcuma aeruginosa rhizome has an impact on apoptosis and PARP-1 expression of hepatocytes in male mice.METHODSEighty eight male Balb C mice were divided into 10 groups treated respectively with Curcuma aeruginosa rhizome cloroform extract, methanol extract, essential oil, infusion, and press juice, at dosages of 0.004g/kgBW and 0.06g/kgBW, and 1 control group. The treatment was given orally once a day for 10 days and on the 11th day, the research animals were sacrificed, and their liver taken for histopathologic slide preparation with Apopteq Detection Kit, and immunofluorescence. Compounds in Curcuma aeruginosa rhizome were analyzed with gas chromatography/mass spectrometry. The data obtained were analyzed by one-way ANOVA, and Partial Least Squares to determine which compounds had an impact on murine hepatocytes.RESULTSThe result of one way ANOVA showed that the chloroform groups at dosages of 0.004g/kgBW and 0.06g/kgBW showed the highest apoptosis of mice’s hepatocytes (p<0.05). There were significant differences in PARP-1 expression between control and treatment groups. The highest PARP-1 expression was in the essential oil group at a dosage of 0.06g/kg BW (p<0.05).CONCLUSIONCurcuma aeruginosa rhizome given to mice orally causes necrosis of mice’s hepatocytes.
PELATIHAN ON-LINE PEMBUATAN PERANGKAT HAND SANITIZER BERBAHAN NON-ALKOHOL PADA PEMUDA BINAAN Mochamad Lazuardi; Hani Plumeriastuti; Eka Pramyrtha Hestianah
Jurnal Layanan Masyarakat (Journal of Public Services) Vol. 4 No. 2 (2020): JURNAL LAYANAN MASYARAKAT
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jlm.v4i2.2020.377-384

Abstract

Training on non-alcohol based hand sanitizer (HS) was conducted for assisted youth in five districts, namely Gresik, Bojonegoro, Lamongan, Probolinggo and Nganjuk for three days. In the training, competency strengthening was carried out, namely theory and practicum, ending with evaluation of activities. During the implementation of theory and practicum, training materials were provided using social media facilities in the form of WhatsApp and e-mail. Specifically for coordination with participants, a training group was created using social media. The results of the training evaluation turned out that in terms of strengthening the competence of knowledge in making HS and working mechanisms of HS, it was increasing. In terms of straightening the meaning of HS production to be more focused according to the basis of science or good manufacturing practices (GMP). Thus, youth assisted with criteria include youth who are able to pass on knowledge to all young people in their respective districts. Furthermore, the knowledge and skills of the assisted youth in making HS based on non-alcoholic substances will be more agile and targeted. Thus, in reality, the training on making non-alcohol based HS has made the assisted youths become pioneers at the end of the line to pass on their knowledge. The conclusion in the implementation of this training is that the assisted youth are capable and ready to make non-alcohol-based HS as well as being ready to produce for a small scale while showing real work to be transmitted (98%). abstrakTelah dilakukan pelatihan pembuatan hand sanitizer (HS) berbasis non-alkohol terhadap pemuda binaan di lima kabupaten yaitu Gresik, Bojonegoro, Lamongan, Probolinggo dan Nganjuk selama tiga hari. Dalam Pelatihan tersebut dilakukan pemberian penguatan kompetensi yaitu teori dan praktikum diakhiri dengan evaluasi kegiatan. Selama berlangsung pelaksanaan teori dan praktikum, materi pelatihan diberikan menggunakan fasilitas media sosial berupa whatsapp dan e-mail. Khusus koordinasi dengan peserta dilakukan pembuatan grup pelatihan menggunakan media sosial. Hasil evaluasi pelatihan ternyata dari segi penguatan kompetensi pengetahuan pembuatan HS dan mekanisme kerja HS, makin meningkat. Segi pelurusan pengertian produksi HS menjadi makin terarah sesuai basis ilmu pengetahuan atau cara pembuatan obat yang baik (CPOB). Dengan demikian pemuda binaan dengan kritetria termasuk pemuda yang sanggup menularkan ilmu ke seluruh masyarakat muda di kabupaten masing-masing. Lebihlanjut pengetahuan dan ketrampilan pemuda binaan dalam membuat HS berbasis bahan non-alkohol makin cekatan dan terarah. Dengan demikian seacara nyata pelatihan pembuatan HS berbasis non-alkohol telah menjadikan pemuda binaan sebagai pelopor di ujung garis untuk menularkan ilmunya. Kesimpulan dalam pelaksanaan pelatihan ini adalah para pemuda binaan telah sanggup dan siap membuat HS berbasis non-alkohol sekaligus siap berproduksi untuk skala kecil sambil memperlihatkan karya nyata untuk ditularkan (98 %).
HISTOPATHOLOGICAL APPEARANCE OF THYMUS ON BROILER UNDER CHRONIC HEAT STRESS Ratna Andriani; Arimbi Arimbi; Dadik Rahardjo; Hani Plumeriastuti; Djoko Legowo; Eka Pramyrtha Hestianah
Journal of Basic Medical Veterinary Vol. 8 No. 1 (2019): Journal of Basic Medical Veterinary, June 2019
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v8i1.19987

Abstract

The aim of this research was to know histopathological change of broiler’s thymus which exposed by chronic heat stress. The results revealed that there were negative impact on immune organ (thymus). Broiler that exposed to chronic heat stress showed significantly increased number of heterophil and apoptosis histiocytes and cell depletion. There were 20 broilers that divided into 2 groups, each group consist of 10 broiler. Control group (P0) reared at temperature 24-28oC and humidity 40-55% and Heat Stress group (P1) reared at temperature 36-40oC on the litter housing system at Experimental Animals Laboratory, Faculty of Veterinary Medicine, Universitas Airlangga. Before the treatments, broiler had been reared from day-old-chicks to 21-day-old, then continued with heat stress condition from day 22 to 42. Thymus was collected on day 42 to examine histopathological change of thymus and analyzed using Mann-Whitney Test. The results showed significant difference (P<0.05) between Control group and HS (Heat Stress) group by increased number of heterophil, apoptosis histiocytes and cell depletion.
PENGARUH PEMBERIAN PROPOLIS LEBAH TERHADAP GAMBARAN HISTOPATOLOGI HEPAR MENCIT (Mus musculus) BETINA YANG DIPAPAR LOGAM BERAT PB ASETAT [Pb(C2H3O2)2] Sartika Dewi Rachmani; Erma Safitri; Roesno Darsono; Eka Pramyrtha Hestianah; Hani Plumeriastuti; Kuncoro Puguh Santoso
Journal of Basic Medical Veterinary Vol. 8 No. 1 (2019): Journal of Basic Medical Veterinary, June 2019
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v8i1.19988

Abstract

Lead acetate in the hepatobiliary system may cause peroxidation catalysis of unsaturated fatty acids, reduce nitrogenoxide and increase hydroxyl radical. Lead acetate produces oxidative stress characterized by free radical formation and inhibits lipid peroxidation. Giving antioxidants can neutralize free radicals from the detrimental effects that arise on the process or excess oxidation reactions. The purpose of this research was to find out how the effect of bee propolis on histopathologic images of hepatic mice (Mus musculus) of females exposed to lead acetate [Pb(C2H3O2)2]. The subjects were 25 mice (Mus musculus) mushulus of BALB/C strain with average weight 25-30 gram and 8 weeks old, divided into 5 treatment groups, each consisting of 5 heads per group. The K- group was given a Tween 80 solution at a doses of 0.5 mg/kgBW for 20 consecutive days. The K+ group, which was given only lead acetate at a doses of 10 mg/kgBW orally for 10 days. P1, P2, and P3 were given 10 mg/kgBW lead acetate solution orally for 10 days. The following 10 days were given bee propolis with doses of P1 200 mg/kgBW, P2 400 mg/kgBW, and P3 800 mg/kgBW. On the 21th days the mice were dissected, to observe the extent of the damage. All data were performed using a statistical test with Kruskal Wallis test and if there was a marked difference between treatment groups (p<0.05), then the Mann-Whitney test was followed. The results obtained that bee propolis can repair hepatic cell damage in mice (Mus musculus) of females exposed to lead acetate. Increased dose of bee propolis is ineffective in repairing hepatic cell damage in mice (Mus musculus) of females exposed to lead acetate.
The Effect of Mimosa Pudica Root Extract on Cerebrum Histopathological of Rattus Norvegicus Induced with Naja Sputatrix Venom Roselia Yuliani Permatasari; Eka Pramyrtha Hestianah; Djoko Legowo; Kadek Rachmawati; Zainal Arifin
Journal of Basic Medical Veterinary Vol. 11 No. 1 (2022): Journal of Basic Medical Veterinary, June 2022
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v11i1.36485

Abstract

The aim of this study was to know the effect of Mimosa pudica root extract on histopathological appearance of Rattus norvegicus brain induced by Naja sputatrix venom. Thirty rats were divided into 5 groups. There were 2 control groups and 3 treatment groups, which was given 250, 500, and 1000 mg/kg BW of Mimosa pudica root extract orally. The first 7 days each group was adapted to the environment. On the 8th day, the treatment was started by injecting Naja sputatrix LD50 (0,13 L/gram BW) IM in gluteus muscle, continued with giving Mimosa pudica root extract orally for the treatment groups 5 minutes after venom injection. 6 hours after the last treatment, rats were killed by cervical dislocation, injected with formalin 10% in the heart, then necropsied. Histopathological evaluation was done to score brain damage based on meningitis, perivascular cuffing, and necrotic cells using HE stain with 1000x magnification. The result showed 1000 mg/kg BW dosage of Mimosa pudica root extract can reduce brain damage based on meningitis, perivascular cuffing, and necrotic cells in Rat (Rattus norvegicus) caused by Naja sputatrix venom and gave significant difference (p < 0.05) among the treatment groups.