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Altering physiological conditions and semen acidity by manipulating dietary cation-anion difference and fish oil supplemenation of Garut breed rams Hidayat, Rahmat; Toharmat, T.; Boediono, A.; Permana, I.G.
Indonesian Journal of Animal and Veterinary Sciences Vol 14, No 1 (2009)
Publisher : Indonesian Animal Sciences Society

Contribution of Garut breed sheep as protein resources is not optimal yet.Â Acceleration of population growth such as increasing of female offspring is neccesary.Â This study was carried out to obtain information regarding the effect of dietary cation-anion difference (PKAR: meq [(Na + K) â (Cl+S)/100 g of dry matter]) and fish oil supplementation on acidity of blood and semen, blood gas and plasma and semen mineral. The dietary treatmens were as follows:Â Â RN0= basal ration (PKAR +14) without fish oil, RNI= basal ration (PKAR +14) with 3% fish oil, RB0= base ration (PKAR +40) without fish oil, RBI= base ration (PKAR +40) with 3% fish oil, RA0= acid ration (PKAR -40) without fish oil, and RAI= acid ration (PKAR -40) with 3% fish oil.Â The rations contained 150 ppm of zinc and were offered to 18 of Garut rams.Â The result indicated that blood pH, pCO2, and pO2 were not affected by PKAR and fish oil supplementation, but cHCO3 and cBase were affected (P<0.05).Â PKAR and fish oil supplementation affected (P<0.01) plasma Mg and S, but did not affect K, Na, Zn and Cl.Â Semen pH after day 28 of experimental period were highly significant different and that followed PKAR pattern.Â In conclution, PKAR affected cBase, cHCO3, concentration of Mg and S plasma and semen pH of Garut rams.Â The result suggested that PKAR could be applied to manipulate physiological condition and semen pH. Key Words: PKAR, Physiological Condition, Semen, Garut Ram

Effect of fermented Jatropha curcas meal combined with enzymes on metabolizable energy, retention of N, P, Ca and digesteble crude fiber Yusriani, Yenni; Toharmat, T.; ., Sumiati; Wina, E.; Setiyono, A.
Indonesian Journal of Animal and Veterinary Sciences Vol 16, No 3 (2011)
Publisher : Indonesian Animal Sciences Society

Jatropha curcas meal (JCM) contain high protein, but its utilization as feed ingredient is limited by the presence of several anti nutritive and toxic compounds. A research has been conducted in the Faculty of Animal Husbandry IPB to determine the values of metabolizable energy, crude fiber digestibility, retention of nitrogen, calcium and phosphorous of JCM fermented using Rhizopus oligoporus or of fermented JCM suplemented with enzymes on chickens. In this experiment 25 of ten weeks old chickens were used (20 chickens were fed experimental diets, and 5 chickens were used to measure endogenous energy). A Completely Randomized Design (CRD) with 5 treatments and 4 replications was used in this experiment. The experimental diets were R0 = basal diet without JCM; R1 = the diet contained unprocess JCM 5%; R2 = the diet contained fermented JCM 5%+ cellulase 20.000 U/kg; R3 = the diet contained 5% of fermented JCM + phytase 1000 FTU/kg; R4 = the diet contained 5% of fermented JCM+ cellulase 20.000 U/kg + phytase 1000 FTU/kg. The parameters measured were Metabolizable Energy (ME, Men, TME, TMEn) digestibility of crude fiber and retention of calcium, phosphorus and nitrogen. The result indicated that fermented JCM suplemented enzym phytase, cellulase as well as enzym combination increased metabolizable energy. Nitrogen retention was improved highest significantly (P < 0.01) by feed of fermented JCM suplemented enzyme compared to control diet or diet containing untreated JCM. The fermented JCM suplemented with phytase enzyme (R3) gave the highest nitrogen retention (76.73%.). The highest value of digested crude fibre (24.65%) was on the diet containing fermented JCM suplemented with cellulase (R2). The calcium retention improved significantly (P < 0.05) with diet containing fermented JCM supplemented enzyme compared to that of the diet containing untreated JCM. It can be concluded that fermented JCM supplemented enzymes cellulase, phytase and its combination increased the value of metabolizable energy, digestibility of crude fiber and nitrogen retention. Key Words: Chicken, JCM Fermented, Enzymes, Metabolic Energy

Performance of Garut breed rams fed diets containing various cation-anion difference with or without fish oil supplementation Hidayat, Rahmat; Toharmat, T.; Boediono, A.; Permana, I.G.
Indonesian Journal of Animal and Veterinary Sciences Vol 16, No 3 (2011)
Publisher : Indonesian Animal Sciences Society

This study was carried out to evaluate the effect of dietary cation-anion difference (DCAD) and fish oil supplementations on dry matter intake (DMI), dry matter digestibility (DMD) and organic matter digestibility (OMD), weight gain, as well as the acidity of urine in Garut breed rams. The experiment was done based on randomized group design with 6 treatmentsÂ and 3 groups. The dietary treatments were as follows: R0= basal ration (DCAD +14) without fish oil, R1 = basal ration (DCAD +14) with 3% fish oil, R2 = base ration (DCAD +40) without fish oil, R3 = base ration (DCAD +40) with 3% fish oil, R4 = acid ration (DCAD -40) without fish oil, and R5 = acid ration (DCAD -40) with 3% fish oil. All rations contained 150 ppm of zinc and were offered to 18 of Garut breed rams. The results indicated that DCAD +40 and -40 decreased DMI significantly.Â The ration with DCAD +40 had the lowest DMI. Fish oil supplementation decreased DMI. No differences were observed for DMD and OMD.Â DCAD +40 and -40 decreased body weight of rams. However, the body weight of rams was very low. Variation of urine pH followed the DCAD pattern. It was concluded that DCAD +40 and -40, as well as fish oil supplementations decreased DMI, body weight and urine pH followed the DCAD pattern. Keys Words: Cation, Anion, Body Weight, Digestibility, Garut Breed Rams

The potential of sugar cane juice as the liquid supplement and phytase enzyme carrier for poultry by in vitro Widjaja, Ermin; Toharmat, T.; Santoso, D.A.; ., Sumiati; Ridla, M.; Iskandar, S.
Indonesian Journal of Animal and Veterinary Sciences Vol 16, No 4 (2011)
Publisher : Indonesian Animal Sciences Society

Most of the components of poultry feed (80%) of grains and meal that contains phytic acid which has anti-nutritional factor because it can bind minerals and reduce its availability. Phytic acid can be hydrolyzed by the enzyme phytase. Phytase enzyme naturally found in sugar cane juice, but its use as poultry feed supplements have not been done. The study was conducted using sugar cane juice PS 851 from Jatiroto PTPN XI, Lumajang, East Java in order to get the information potential of sugar cane juice as a liquid supplement and phytase enzyme carrier for poultry viewed from the aspect of nutrient content of sugarcane juice and phytase activity in the release rate of phosphorus. Research conducted at the Faculty of Animal IPB for 10 months. The rate of hydrolysis of phytase on P was tested using rice bran as a substrate. Sugar cane juice is added to the 2.5% level, using 4-level incubation (1, 2, 3 and 4 hours), each level consisting of 37Â°C and 42Â°C; pH 2; pH 4.5 and pH 5 with three replications. Study using a Two Factors Experiments in Completely Randomized Design and it was continued by DMRT test. P release rate was measured by spectrophotometry. The results showed that the sugar cane juice has a phytase activity of 0.0766 U / ml, brix level of 22.15%, containing water 73.03%, protein 0.47%, crude fiber 6.43%, minerals Ca 0.03%, P 0,02%, Co 0.14 mg / l, Fe 1.8 mg/l, Mn 1.55 mg/l, Zn 1.37 mg/ l, Cu 0.19 mg/ l, Se 12.63 mcg/100 g, vitamins B3 5.26 mg/100 g, C 0.72 mg/100 g, E 0.08 mg/100 g, sucrose 32.42%, fructose 2.41%, galactose 2% and glucose 1.58%. Supplementation of 2.5% sugar cane juice can increase the P release rate of 112-235% at optimum conditions of pH 5, at 37Â°C with a long incubation period of 1-4 hours. Key Words: Sugar Cane Juice, Phytase, Phosphorus

BIOCONVERSION PROPERTY OF TEA LEAVES WASTE BY Aspergillus niger AS FUNCTIONAL FIBER TO DECREASE BLOOD LIPID Hernaman, I.; Kamil, K.A.; Tanuwiria, U.H.; Lestari, E.S.; Toharmat, T.
Journal of the Indonesian Tropical Animal Agriculture Vol 35, No 4 (2010): (December)
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.35.4.227-231

This research was aimed to find out the change of tea leaves waste fiber on lipid absorption afterbeing fermented by Aspergillus niger and also to be expected as a model to gain healthy livestockproduct. Tea leaves waste was fermented by Aspergillus niger during 6 day. Fifteen of rats with initialweight of 41.17±4.05 g were fed ad libitum by diets containing different levels of unfermented andfermented tea leaves waste as follows: basal diet (R1), unfermented tea leaves waste 5% (R2),unfermented tea leaves waste 10% (R3), fermented tea leaves waste 5% (R4), fermented tea leaveswaste 10% (R5). The results indicated that bioconversion of tea leaves waste in diet (R4 and R5)decreased almost all of fiber fractions (NDF 75.55%; ADF 57.17%; celluloses 26.74%; lignine 31.19%),except hemicelluloses 18.38%, while the treatment did not change the lipid binding rate (2.07 vs 1.98).Also, the result showed that rats fed with diets containing fermented tea leaves waste (R4 and R5) hadreduced cholesterol (86.25;87.4 mg/dl) and triglyceride (47.06; 46.03 mg/dl), but not on HDLcholesterol(29.28; 26.51 mg/dl). It was concluded that bioconversion property of tea leaves waste byAspergillus niger changed fiber fraction and decreased blood lipid.

THE NUTRITIVE VALUES OF PUFA-CONCENTRATE SUPPLEMENTED WITH YEAST AND Curcuma xanthorrhiza Roxb STORED IN SEVERAL WEEKS Sulistyowati, E.; Sudarman, A.; Wiryawan, K. G.; Toharmat, T.
Journal of the Indonesian Tropical Animal Agriculture Vol 40, No 1 (2015): March
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.40.1.11-22

The PUFA (polyunsaturated fatty acid)-concentrates containing fatty acid sources (roasted corn grain,roasted soy bean meal, and corn oil) was designated as PUFA- concentrate for dairy goat. There werefour PUFA-concentrates, no supplement (PC0), 0.5% or 5 g yeast (PCY), 2% or 20 g curcuma powder(PCC), and 0.5% or 5g yeast with 2% or 20g curcuma powder (PCM). These PUFA-concentrates wereevaluated for nutrients and fatty acid contents during 2, 4, and 6 weeks of storage. The application oftreatments utilized in this research was completely randomized design with repeated measurement andsplit plot statistical analysis. Results showed that the contents of dry matter, organic matter, ether extract,crude protein, N-free extract, gross energy, acid detergent fiber, Ca, P, and Saccharomyces cereviseaewere significantly (P<0.05) remained stable as caused by unchained moisture of PUFA-concentrate withcombined supplements (Y5C20) in the 6 weeks of storage. The total PUFA (P), P/S, monounsaturatedfatty acid (MUFA), and long chained fatty acid contents tended to be high in PUFA-concentrate with 2%or 20 g curcuma powder. Whereas, the PUFA-concentrate with a combination of 0.5% or 5 g yeast and2% or 20 g curcuma powder was higher in unsaturated (U) fat and the ratio of U/S. In conclusion, combining all nutrient performances during the storage of 2 to 6 weeks, the PUFA-concentrate with0.5% or 5 g yeast and 2% or 20 g curcuma powder was considered nutritionally good.

QUALITY OF MILK FATTY ACID DURING LATE LACTATION IN DAIRY GOAT FED ON PUFA-DIET SUPPLEMENTED WITH YEAST AND Sulistyowati, E.; Sudarman, A.; Wiryawan, K.G.; Toharmat, T.
Journal of the Indonesian Tropical Animal Agriculture Vol 38, No 4 (2013): (December)
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.38.4.247-256

Yeast and curcumin of C. xanthorrhiza Roxb could be added into concentrate containingpolyunsaturated fatty acid (PUFA) to improve milk fatty acid quality of dairy goat. There were fivetreatments (PD0: PUFA- diet with no additive; PDA: PUFA- diet with 3 Asifit tablets; PDY: PUFA- dietwith 5 g yeast; PDC: PUFA- diet with 20 g curcuma powder; and PDM: PUFA- diet with a mixture of 5g yeast and 20 g curcuma powder) applied onto 20 dairy goats during late lactation (4.6 ± 0.55 monthsof lactation). The treatments were allocated according to a completely completely randomized blockdesign. Results demonstrated that diet containing PUFA supplemented with 5 g yeast and 20 g curcumawere high in total fatty acid, medium chain fatty acid (MCFA), and long chain fatty acid (LCFA). Milkof goats with this treatment showed high in mono unsaturated fatty acid (MUFA) and MUFA; while itwas low in short chain fatty acid (SCFA), n6/n3 ratio, and atherogenicity index. These qualities wereoptimally considered good in terms of healthier product. Therefore, the PUFA- diet with 5 g yeast and20 g curcuma was a reasonable choice to be applied for dairy goat.

ASSESSMENT OF METHANE ESTIMATION FROM VOLATILE FATTY ACID STOICHIOMETRY IN THE RUMEN IN VITRO Jayanegara, A.; Ikhsan, I.; Toharmat, T.
Journal of the Indonesian Tropical Animal Agriculture Vol 38, No 2 (2013): (June)
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.38.2.103-108

Rumen microbes breakdown feed to produce volatile fatty acids (VFA), carbon dioxide, ammoniaand methane (CH4). Metabolic hydrogen in the form of reduced protons is used during CH4 formation aswell as during VFA synthesis. Therefore, VFA concentration in the rumen may stoichiometrically berelated to CH4 emission. The aim of this study was to evaluate methane emission between experimentaland model estimates. Two stoichiometrical models for predicting CH4 from VFA were assessed, i.e.Moss et al. (2000) and Hegarty and Nolan (2007) models. The data sets were obtained from a publishedliterature. Samples used were leaves from 27 tropical plant species. Prediction error was conducted bycomputing root mean square prediction error (RMSPE). Results showed that estimation model of Mosset al. (2000) had lower RMSPE value, i.e. 8.01%, than that of Hegarty and Nolan (2007) model, i.e.10.73%. Variation of methane emission, i.e. the low or high methane can be estimated by VFAcomposition with a sufficient accuracy. Adjusment by considering H2 recovery lowered the biassignificantly. It can be concluded that Moss model had better accuracy in predicting CH4 emission fromVFA composition than that of Hegarty and Nolan model.

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