Tatik Wardiyati
Jurusan Budidaya Pertanian Fakultas Pertanian Universitas Brawijaya

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Selection In vitro of Granola Potato M1 Againts Bacteria Wilt Disease Ralstonia solanacearum Asta, Hidayat; Wardiyati, Tatik; Maghfoer, Mochamad Dawam
Journal of Tropical Plant Protection Vol 1, No 1 (2012)
Publisher : University of Brawijaya

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Abstract

AbstracBacterial wilt disease caused by Ralstonia solanacearum is an important disease and could decreased yield up to 80%. Generally, bacterial disease is more difficult to control than other diseases. Moreover, there are no chemicals that can be used to control this pathogen. The approach through genetic improvement by using Ethyl Methane Sulfonate and in vitro selection are procesess to generate variants resistant against bacterial wilt on potato plants. Granola potato M1 is the result of treatment with mutagen Ethyl Methane Sulfonate. Based on the results of selection in vitro of potato plantlets M1 Granola with suspense of Rs 9 × 1010 CFU / ml, were can be induced E9 three potato plantlets are rather susceptible to  Ralstonia solanacearum through the regenerated node and E6 and E8 treatment can be induced moderately resistant planlets against Ralstonia solanacearum. Keyword: EMS, Granola, Ralstonia solanacearum
PENGARUH DEFOLIASI DAN DETASSELING TERHADAP HASIL TANAMAN JAGUNG (Zea mays L.) Shodikin, Aris; Wardiyati, Tatik
PLANTROPICA: Journal of Agricultural Science Vol 2, No 1 (2017)
Publisher : Department of Agronomy, Faculty of Agriculture, Brawijaya University

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Abstract

Tanaman jagung (Zea mays L.) merupakan komoditi strategis yang dapat digunakan sebagai pengganti beras. Pada tahun 2014, produksi jagung nasional mengalami peningkatan sebesar 2,81%. Defoliasi dan detasseling merupakan teknik budidaya yang sudah dilakukan oleh petani secara turun menurun yang bertujuan untuk memenuhi kebutuhan pakan ternak. Oleh karena itu, tujuan penelitian ini yaitu untuk memberikan pengetahuan lebih lanjut tentang pengaruh serta waktu defoliasi dan detasseling yang tepat terhadap pertumbuhan dan hasil tanaman jagung. Penelitian dilaksanakan pada bulan Desember 2015 – Maret 2016 di Desa Sumberarum, Kecamatan Wates, Kabupaten Blitar. Bahan yang digunakan dalam penelitian yaitu benih jagung varietas NK 99, pupuk urea, pupuk ponska dan pupuk petroganik. Penelitian menggunakan Rancangan Acak Kelompok (RAK) dengan 7 perlakuan dan 4 ulangan. Data dianalisis menggunakan analisis ragam (ANOVA), apabila terdapat pengaruh nyata dilanjutkan dengan uji lanjut Duncan Multiple Range Test (DMRT) pada taraf nyata 5%. Hasil penelitian menunjukkan defoliasi dan detasseling tidak berpengaruh nyata terhadap semua parameter pengamatan pertumbuhan dan hasil, kecuali parameter diameter tongkol. Perlakuan yang diterapkan tidak memberikan pengaruh nyata karena waktu pelaksanaan defoliasi dan detasseling pada penelitian ini terlalu jauh dari fase tasseling. Sedangkan pada diameter tongkol disebabkan oleh morfologi bentuk tongkol jagung yang tidak seutuhnya bulat sehingga mempengaruhi hasil analisis ragam. Perlakuan defoliasi dan detasseling tidak menimbulkan efek negatif terhadap hasil tanaman jagung sehingga yang dilakukan petani selama ini sudah benar.
Evaluation of Scar18 Marker Linked to B-Carotene for Early Screening of Mango (Mangifera indica L.) Progenies Wardiyati, Tatik; Arumingtyas, Estri Laras; Roviq, Mochammad
AGRIVITA, Journal of Agricultural Science Vol 32, No 3 (2010)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v32i3.23

Abstract

A breeding program of Mango (Mangifera indica) was organized by a team of Brawijaya University since 2006 by cross pollination between Arumanis 143 (green skin) with yellow skin cvs. of Carabao, Haden, Podang and Swarnarika in vice versa. As early identification a molecular evaluation was conducted. Measurement were on carotene content, DNA analysis using PCR and sequencing. The result showed that mango cultivars having orange or yellowish skin contain carotene higher than those mango cultivar having green skin. SCAR18 marker as a sign on the presence of beta (B) locus in tomato was not related with the differences of beta carotene accumulation in those manggo cultivars. SCAR18 marker that was amplified in mango produced specific amplimer of 320 bp in length. The similarity of SCAR18320 sequence obtaining by sequences alignment among five parental cultivars and 12 hybrids was up to 100%. SCAR18320 sequences that were generated by SCAR18 markers as predicted beta (B) loci had no significant similarity with sequences database relating to beta (B) gene in NCBI. SCAR18320 match to 19 Ty3-gypsy retrotransposons and others sequences related transcriptional regulator in more dissimilar sequences (discontiguous megablast) category.Keywords: SCAR18, ? carotene, skin color, Mangifera indica
RHIZOME YIELD OF TEMULAWAK (Curcuma xanthorrhiza Roxb.) AT N, P, K VARIOUS LEVEL AND N, K COMBINATION Nihayati, Ellis; Wardiyati, Tatik; Sumarno, Sumarno; Retnowati, Rurini
AGRIVITA, Journal of Agricultural Science Vol 35, No 1 (2013)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v35i1.263

Abstract

The role of N, P and K on temulawak have not been clear enough to explain the need  in  temulawak  rhizome in order to get high yield. The experiment was conducted in a screen house in  Malang East Java with an altitude of 500 m asl. from Desember 2010 - August 2011. There were 4 experiments in series, consisting of N, P, K various level and N,K alone and combination on the growth and  quantity  of rhizome arranged in  randomized block design comprising 7 levels of fertilizer per plant with 3 replications. The treatments of urea (N), SP36 (P) consisted of 0, 1,25, 2,5, 3,75, 5, 6,25 and 7.5 g. The KCl (K) comprised 0, 1,5, 3, 4,5, 6, 7,5 and 9 g. The N, K alone and combination treatments consisted of (No fertilizer, N, K, NK g /plant). The results of this research were N, P, K optimum dose of each N 6.25 g N / plant P2O5/tan P 6.25 g, K 7.5 g K2O / plant increased temulawak rhizome dry weight. Combination 4.5 g N + 2.8 g K / plant could increase temulawak rhizome dry weight by 33% compared to the addition of N and 220% when compared to the addition of a single K harvesting age 6 months. Keyword : Curcuma xanthorriza, rhizome, N, P, K.
YIELD AND CURCUMIN CONTENT STABILITY OF FIVE UB CLONES OF TEMULAWAK (Curcuma xanthorriza Rox.) Wardiyati, Tatik; Kuswanto, Kuswanto; Azizah, Nur
AGRIVITA, Journal of Agricultural Science Vol 34, No 3 (2012)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v34i3.128

Abstract

Interactions between genotype and environment  is affect yield and curcumine content of temulawak (Curcuma xanthorrhiza Rox). The aims of the study was to determine stability of yield and curcumine content in diffrent enviromental condition. Field experiment was conducted during 12 month of year 2010, and Randomized block design with three replications was applied at four locations : Sragen, Malang, Pasuruan and Sumenep.  The materials tested consists of  five clones : UB1, UB2, UB3, UB4, UB5 included control ( Balitro clone). The  results showed that the stability of the rhizome yield in each locations , achieved by UB3 and UB4 clones. The highest rhizome weight was obtained by UB2  at 34,63 t/ha, while the constant high curcumin content was obtained by UB4. Clones UB1 and UB5 suitable to be developed on marginal lands and clone UB2 adaptive on high fertility  environment.   Key words :temulawak, adaptation, clones
APLIKASI TEKNOLOGI BUDIDAYA RAMAH LINGKUNGAN TERHADAP KUALITAS BUAH STROBERI Sumayku, Bertje R.A.; Wardiyati, Tatik; Afandhi, Aminudin; Polii-Mandang, Jeanny
EUGENIA Vol 23, No 1 (2017)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35791/eug.23.1.2017.15409

Abstract

ABSTRACT   This study aimed to compare the application of environment-friendly cultivation technology on the quality of strawberries. The experimental design using a factorial in a randomized block design (RAK), with 2 factors, namely: 1). Farmers treatment technology that chicken manure 20 t / ha + Urine Rabbits 1: 5 liters of water; 2). Treatment T. koningii 15 g / kg of soil in chicken manure 5 tons / ha). Data were analyzed by t test statistics, if different continued with test Duncun at 0.05. Research results show that treatment of PP (T. koningii Technology) has Vitamin C is 27.1 mg / 100g, higher than the Treatment PT (Technology Farmers) is 22.5 mg / 100g. The vitamin C content of the variables were not significantly different between treatments, while the variable texture of the fruit, and sugar content were significantly different. Keywords: applications agriculture environmentally-friendly, strawberry fruit quality
ENHANCEMENT IN EFFECTIVENESS OF ANTAGONISTIC MICROBE BY MEANS OF MICROBIAL COMBINATION TO CONTROL Ralstonia solanacearum ON POTATO PLANTED IN MIDDLE LATITUDE Rosyidah, Anis; Wardiyati, Tatik; Abadi, Abdul Latief; Maghfoer, Moch. Dawam
AGRIVITA, Journal of Agricultural Science Vol 35, No 2 (2013)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v35i2.285

Abstract

One of the common problems hampering the cultivation of potatoes in middle latitude is the presence of bacterium Ralstonia solanacearum commonly known to cause wilt disease, by which crop failure might be caused when serious attack occurs. The objectives of the research were to obtain the application of antagonistic microbes to inhibit the wilt disease caused by R.solanacearum and to increase the growth and yield of potato in middle latitude. The research was conducted from July to October 2012. Antagonistic microbes were used in this research to inhibit the bacterial wilt disease caused by R.solanacearum on potato planted in Bumiaji, Batu. Trichoderma viride, Streptomyces sp. and Pseudomonas fluorescens isolates were selected to be applied as treatments either singly or in combination. Randomised block design was applied on the treatment group with three replications. The results showed that the single application of Pseudomonas fluorescens or combined application of Streptomyces sp. and Trichodermaviride + Streptomycessp. was capable of extending incubation period 4 to 7 days and reducing disease incidence  44.85% - 50.09%, reducing disease intensity up to 61.23 - 72.77%, reducing the population R.solanacearum up to 7.28 - 97.88%, increasing the number of leaves and the marketable yield as much as 67.96 - 81.98%.Keywords: control, Ralstonia solanacearum, antagonistic microbes, middle latitude
Pengaruh Pupuk Daun dan GA3 terhadap Pertumbuhan dan Hasil Tanaman Bunga Potong Krisan (Chrysanthemum morifolium) Reginasari, Immanuelita Sarah; Roviq, Mochammad; Wardiyati, Tatik
Jurnal Produksi Tanaman Vol 8, No 5 (2020)
Publisher : Jurusan Budidaya Pertanian Fakultas Pertanian Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/1406

Abstract

Bunga potong memiliki nilai estetika yang tinggi dan memberikan keindahan untuk dekorasi atau hiasan ruang. Bunga Krisan (Chrysanthemum morifolium) memiliki warna yang menarik dan bentuk bunga yang indah sehingga tanaman krisan menjadi salah satu tanaman hias yang cukup digemari di Indonesia. Tanaman krisan dapat diproduksi menjadi bunga potong maupun bunga pot. Nilai penting untuk pemasaran bunga krisan potong ialah panjang tangkai (Kazaz et al., 2010). Pemberian pupuk pada tanaman dapat diberikan melalui daun. Zat Pengatur Tumbuh (ZPT) juga dapat meningkatkan hasil tanaman krisan dan umur panen. Pemberian ZPT terhadap tanaman krisan, mampu memberikan hasil yang lebih baik dibandingkan dengan krisan yang tidak diberikan perlakuan apapun. Penelitian dilaksanakan di Desa Beru, Kota Batu, Provinsi Jawa Timur di dalam greenhouse. Penelitian dilaksanakan bulan Januari hingga April 2019. Penelitian menggunakan Rancangan Acak Lengkap Faktorial (RALF) dengan dua faktor. Faktor pertama pupuk daun rosasol N pada fase vegetatif dan rosasol P pada fase generatif, faktor kedua asam giberelin (GA3). Perlakuan diulang 3 kali. Hasil penelitian menunjukkan bahwa perlakuan 250 ppm GA3 dan 3 g/L pupuk daun memberikan hasil yang lebih baik dibandingkan dengan perlakuan yang lain.
Pengaruh Konsentrasi Nutrisi Hidroponik Terhadap Pertumbuhan dan Hasil Tanaman Sawi (Brassica juncea L.) Rosita, Wenny Dwi; Rahayu, Aldhila Putri; Wardiyati, Tatik
Jurnal Produksi Tanaman Vol 9, No 2 (2021)
Publisher : Jurusan Budidaya Pertanian Fakultas Pertanian Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/1517

Abstract

Hidroponik merupakan salah satu teknologi alternatif yang dapat digunakan untuk mengurangi ketergantungan lahan pertanian yaitu bercocok tanaman pada media non-tanah (soil-less agriculture). Hidroponik memiliki banyak keunggulan diantaranya pemakaian pupuk lebih efisien, produksi tanaman lebih tinggi, kualitas tanaman lebih baik, beberapa tanaman dapat di budidayakan di luar musim dan dapat dilakukan di berbagai tempat pada lahan atau ruang yang terbatas. Budidaya secara hidroponik perlu diberikan larutan nutrisi yang cukup, air, dan oksigen pada perakaran tanaman agar proses pertumbuhan tanaman dapat berlangsung dengan baik.  Pemberian konsentrasi nutrisi yang tepat pada tanaman dapat meningkatkan kualitas dan kuantitas tanaman. Penelitian ini bertujuan untuk memperoleh konsentrasi nutrisi yang paling efisien untuk pertumbuhan tanaman sawi yang sering di konsumsi oleh masyarakat, yatu varietas shinta dan varietas tosakan. Penelitian dilaksanakan di greenhouse Desa Klanderan, Kecamatan Plosoklaten, Kediri pada Januari – Maret 2020. Rancangan yang digunakan berupa Rancangan Acak Kelompok Faktorial (RAKF) dengan 2 faktor. Faktor pertama yaitu konsentrasi nutrisi hidroponik 1050 ppm, 1150 ppm, dan 1250 ppm, sedangkan faktor kedua yaitu varietas sawi sinta dan tosakan. Hasil penelitian menunjukkan perlakuan konsentrasi nutrisi 1150 ppm dan varietas shinta merupakan konsentrasi nutrisi yang paling efisien dengan memberikan respon hasil yang optimal pada pertumbuhan tanaman sawi.