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Theopilus W Watuguly
Program Pendidikan Biologi, Jurusan PMIPA FKIP Dan Program Pendidikan Dokter Universitas Pattimura Ambon, Jl. Dr. Tamaela, Kampus Universitas Pattimura Ambon
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Education Health Professions Immunology & microbiology Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Physics Public Health Social Sciences

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Journal : MEDIA MEDIKA INDONESIANA

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Pengaruh Polifenol Mahkota Dewa Terhadap Proliferasi Sel dan Apoptosis pada Mencit Strain Balb/C yang Diinduksi Benzo(a) Pyrene (BaP) Theopilus W. Watuguly; Indranila KS; Pamela Mercy Papilaya; Edi Dharmana
MEDIA MEDIKA INDONESIANA 2013:MMI VOLUME 47 ISSUE 1 YEAR 2013
Publisher : MEDIA MEDIKA INDONESIANA

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Abstract

ABSTRACT The effect of mahkota dewa polyphenol in the cell proliferation and apoptosis in BaP induced Balb/c ratsBackground: The polyphenol of mahkota dewa (Phaleria macrocarpa [Scheff.] Boerl) has the potency as antioxidant and anticancer which can handle free radicals, but there has not been extensive research on this. This research is aimed to prove the role of mahkota dewa polyphenol in the cell proliferation inhibition and induct lung carcinogenesis apoptosis in strain Balb/c mice which inducted with benzo(a)pyrene (BaP).Methods: Posttest control group design was carried out among 40 strain Balb/c mice sample, aged 1-2 weeks, weighed 20-30 grams, healthy mice condition. All mice were inducted with BaP and then randomized into 2 groups, as control group and the treatment group. The development of the lung tumor was observed by tissue surgery in the 8th, 17th and 26th week. The data collected were AgNORs, and IHC-TUNEL-apoptosis index dying. The data analysis was conducted using Kruskal-Wallis, Mann-Whitney, One-way ANOVA, and Post hoc test LSD with significance degree of p<α (0.05).Results: The oral administration of mahkota dewa polyphenol showed significantly decreasing cell proliferation, increasing apoptosis index in treatment group in week 8, 17 and 26 (p=0.000). Carcinogenesis incidence for the treatment group week 8 and 26 were 2.32±0.26 and 3.93±0.46, while for the treatment group were 1.88±0.38 and 0.88±0.22 (p=0.000). The cell proliferations for control group week 8 and 26 were 1.57±0.12 and 2.29±0.15, while for the treatment group were 1.53±0.11 and 1.60±0.04 (p=0.000). Apoptosis index for the control group for week 8 was 0.00±0.00 and 0.92±0.22 in week 26, while the treatment group was 1.12±0.71 and 2.02±1.05 (p=0.000).Conclusion: The administration of mahkota dewa polyphenol effectively inhibited the cell proliferation activity and increased apoptosis measured by apoptosis index. Therefore polyphenol has anticancer and antioxidant activities which can inhibit lung carcinogenesis in Balb/c mice.Keywords: Polyphenol, mahkota dewa (Phaleria macrocarpa [Scheff.] Boerl), apoptosis index, strain Balb/c mice, benzo(a)pyrene (BaP).ABSTRAKLatar belakang: Polifenol mahkota dewa (Phaleria macrocarpa [Scheff.] Boerl) berpotensi sebagai antioksidan dan antikanker yang mampu menangkap radikal bebas, namun belum banyak diteliti. Penelitian ini bertujuan membuktikan peran polifenol mahkota dewa dalam menginhibisi proliferasi sel dan menginduksi apoptosis pada mencit strain Balb/c hasil induksi Benzo(a)pyrene (BaP).Metode: Posttest control group design dengan sampel 40 mencit strain Balb/c, umur 1-2 minggu, berat 20-30 g, kondisi mencit sehat. Semua mencit diinduksi BaP kemudian hewan dirandomisasi menjadi 2 kelompok yaitu kelompok kontrol dan kelompok perlakuan polifenol 50 mg. Perkembangan tumor paru diamati dengan pembedahan jaringan pada minggu ke-8, 17 dan 26. Data dikumpulkan meliputi AgNORs, pengecatan IHC-TUNEL-indeks apoptosis. Analisis data dengan Kruskal-Wallis, Mann-Whitney, One-way ANOVA, post hoc test LSD dengan derajat kemaknaan p<α (0,05).Hasil: Pemberian oral polifenol mahkota dewa sebesar 50 mg secara bermakna memperlihatkan penurunan insidens karsinogenesis paru, proliferasi sel, protein Bax dan peningkatan indeks apoptosis, protein p53, Bcl-2, ekspresi caspase 3, 8, 9 pada kelompok perlakuan pada minggu ke-8, 17 dan 26 (p=0,000). Insidens karsinogenesis untuk kelompok kontrol minggu ke-8, dan 26 sebesar 2,32±0,26 dan 3,93±0,46, sedangkan kelompok perlakuan sebesar 1,88±0,38 dan 0,88±0,22 (p=0,000). Proliferasi sel untuk kelompok kontrol minggu ke-8 dan 26 sebesar 1,57±0,12 dan 2,29±0,15, sedangkan kelompok perlakuan sebesar 1,53±0,11 dan 1,60±0,04 (p=0,000). Indeks apoptosis pada kelompok kontrol minggu ke-8 sebesar 0,00±0,00 dan 0,92±0,22 minggu ke-26, sedangkan kelompok perlakuan sebesar 1,12±0,71 dan 2,02±1,05 (p=0,000).Simpulan: Pemberian polifenol mahkota dewa efektif meng-inhibisi proliferasi sel dan menginduksi apoptosis melalui peningkatan indeks apoptosis. Jadi polifenol mahkota dewa memiliki aktivitas antikanker dan antioksidan mampu menghambat karsinogenesis paru mencit Balb/c.
Induksi Polifenol Mahkota Dewa dan Apoptosis Sel Kanker Paru Mencit Strain Balb/C: Analisis pada Up-Regulation Bax dan Down-Regulation Bcl-2 Theopilus W Watuguly; Tjahjono Tjahjono; Martha Kaihena; Syahran Wael
MEDIA MEDIKA INDONESIANA 2012:MMI VOLUME 46 ISSUE 1 YEAR 2012
Publisher : MEDIA MEDIKA INDONESIANA

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Abstract

Mahkota Dewa polyphenol induction and apoptosis cell lung cancer in Balb/c strain mice analysis on up-regulation bax and downregulation Bcl-2Background: This research is aimed to prove the role of Mahkota Dewa polyphenol in the up-regulation Bax and down-regulation in strain Balb/c mice which inducted with benzo(a)pyrene (BaP).Method: Post test control group design with 30 strain Balb/c mice sample, aged 1-2 weeks, weighed 20-30 grams, healthy mice condition. All mice inducted with BaP and then randomized into 2 groups, which were the control group and the treatment group (polyphenol 50mg). The development of the lung carcinogenesis was observed by tissue surgery in the 8th, 17th and 26th week. The data collected include expression examination Bax and Bcl-2. The data analysis was conducted by Kruskal-Wallis, Mann-Whitneyand correlation test of Spearman's with significance degree of p<a (0.05).Result: The oral administration of Mahkota Dewa polyphenol of 50 mg were significantly decreased the occurance of lung carcinogenesis through decreasing of protein Bax and increasing Bcl-2 in treatment group in week 8, 17 and 26 (p=0.000). Protein Bax for the control group for week 8 were 4.04±0.22 and 1.92±0.10 in week 26, while the treatment group were 5.96±0.32 and 4.68±0.22 (p=0.000). Protein Bcl-2 for the control group for week 8 were 5.80±0.32 and 9.64±0.26 in week 26, while the treatmentgroup were 5.12±0.22 and 7.38±0.21 (p=0.000). The Spearman correlation for Bax and Bcl-2 with significancy value of (p=0.000).Conclusion: The administration of Mahkota Dewa polyphenol of 50 mg effectively increased Bax protein expression and decreased Bcl-2 protein expression in mice Balb/c.Keywords: Polyphenol, Mahkota Dewa (Phaleria macrocarpa [Scheff.] Boerl), protein Bax, protein Bcl-2, strain Balb/c mice, benzo(a)pyrene (BaP)ABSTRAKLatar belakang: Penelitian ini bertujuan membuktikan peran polifenol Mahkota Dewa dalam meningkatkan protein Bax dan menurunkan protein Bcl-2 pada mencit strain Balb/c hasil induksi Benzo(a)pyrene (BaP).Metode: Post test only control group design dengan sampel 30 mencit strain Balb/c, umur 1-2 minggu, berat 20-30 gram, kondisi mencit sehat. Semua mencit diinduksi BaP kemudian hewan dirandomisasi menjadi 2 kelompok yaitu kelompok kontrol dan kelompok perlakuan polifenol 50 mg. Perkembangan karsinogenesis paru diamati dengan pembedahan jaringan pada minggu ke-8, 17 dan 26. Data dikumpulkan meliputi pemeriksaan ekspresi Bax dan Bcl-2. Analisis data dengan Kruskal-Wallis dilanjutkan denganuji Mann-Whitney dan uji korelasi Spearman dengan derajat kemaknaan p<a (0,05).Hasil: Pemberian oral polifenol Mahkota Dewa sebesar 50 mg secara bermakna memperlihatkan peningkatan protein Bax dan penurunan protein Bcl-2 pada kelompok perlakuan pada minggu ke-8, 17 dan 26 (p=0,000). Protein Bax pada kelompok kontrol minggu ke-8 sebesar 4,04±0,22 dan 1,92±0,10 minggu ke-26, sedangkan kelompok perlakuan sebesar 5,96±0,32 dan 4,68±0,22 (p=0,000) sedangkan protein Bcl-2 pada kelompok kontrol minggu ke-8 sebesar 5,80±0,32 dan 9,64±0,26 minggu ke-26, sedangkan kelompok perlakuan sebesar 5,12±0,22 dan 7,38±0,21 (p=0,000). Hasil uji korelasi Spearman protein Bax dan protein Bcl-2 dengan nilai significancy (p=0,000).
Co-Authors Aknes Tahya Alfia Sabban Anik Uniarti Bare, Yohanes Beatrixs Rupilu Chrismenda Pay Didik Wahyudi Dominggus Rumahlatu Edi Dharmana Eldo Dandirwalu F Leiwakabessy Febi Stevano Rumangun H Sinay Hanna Kalorbobir Harfalien Tehubijuluw Haslina Kole Icak Darling Rahakbauw Indahsari, Lilin Ika Nur Indranila KS Ine Arini Jecklyn A Lekal Johanis Fritzgal Rehena M Kaihena Maria Nindatu Martha Kaihena Nia Rusfita Titdoy Nindatu, Maria Nurmila Nurmila Pamela Mercy Papilaya Prelly M Tuapattinaya Prelly M.J Tuapattinaya Prelly Tuapattinaya R Malawat Rezza Julian Patty Sari, Dewi Ratih Tirto Silvia E Lopulalan Sintje Liline Sriyanti Imelda Aksamina Salmanu Syahran Wael Syahran Wael Syahran Wael Tjahjono Tjahjono Uniarti, Anik Viesta Sampe Yosep Matruty Yulviany Latuihamallo