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All Journal MEDIA MEDIKA INDONESIANA Majalah Obat Tradisional Jurnal Profesi Medika: Jurnal Kedokteran dan Kesehatan Biopendix: Jurnal Biologi, Pendidikan dan Terapan Science Map Journal JSMARTech : Journal of Smart Bioprospecting and Technology Bioscientist : Jurnal Ilmiah Biologi
Theopilus W Watuguly
Program Pendidikan Biologi, Jurusan PMIPA FKIP Dan Program Pendidikan Dokter Universitas Pattimura Ambon, Jl. Dr. Tamaela, Kampus Universitas Pattimura Ambon
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Education Health Professions Immunology & microbiology Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Physics Public Health Social Sciences

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Aplikasi Ekstrak Kulit Buah Naga Merah (Hylocereus polyrhizus) Pada Nata De Coco Dalam Menurunkan Kadar Kolesterol Total, LDL, Dan Meningkatkan HDL Pada Mencit (Mus musculus) Watuguly, Theopilus Wilhelmus; Uniarti, Anik; Nindatu, Maria
Jurnal Profesi Medika : Jurnal Kedokteran dan Kesehatan Vol 13, No 1 (2019): Jurnal Profesi Medika : Jurnal Kedokteran dan Kesehatan
Publisher : Fakultas Kedokteran UPN Veteran Jakarta Kerja Sama KNPT

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (232.29 KB) | DOI: 10.33533/jpm.v13i1.691

Abstract

Hypercholesterolemic sufferers generally consume conventional drugs using hypolipidemic drugs to reduce cholesterol levels. But this type of drug also has side effects. In addition to consuming hypolipidemic drugs, increased cholesterol levels can be suppressed by regulating dietary patterns by controlling nutrient intake in a balanced manner as needed. Red dragon fruit skin extract (Hylocereus polyrhizus) applied to nata de coco is believed to reduce cholesterol levels because both contain fiber which can be used for dietary needs, and improve the digestive process. The purpose of this study was to determine the application and determine the dose of red dragon fruit skin extract (Hylocereus polyrhizus) in nata de coco in reducing total cholesterol, LDL and increasing HDL in mice (Mus musculus). The type of research used is experimental research using the Pre-Post Test Only Control Group Design research design that uses 18 mices with different doses of 1: 1 and 1: 2 doses. ANOVA statistical test on the results of the study showed that effective treatment in reducing total cholesterol, LDL, and increasing HDL was treated with extracts of red dragon fruit skin (Hylocereus polyrhizus) in a 1: 2 nata de coco. Thus it can be concluded that the higher the dosage given to mice tested it showed a significant reduction in cholesterol levels.
In Silico Study: Potential Prediction of Curcuma longa And Cymbopogon citratus Essential Oil As Lipoxygenase Inhibitor Bare, Yohanes; Indahsari, Lilin Ika Nur; Sari, Dewi Ratih Tirto; Watuguly, Theopilus
JSMARTech: Journal of Smart Bioprospecting and Technology Vol 2, No 2 (2021)
Publisher : JSMARTech

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jsmartech.2021.002.02.75

Abstract

Abstract: Inflammation is the human body response by the injure as a results the inflammation will release LOX. To curve the conditions we use the bioactive from the nature are essential oil from Curcuma longa and Cymbopogon citratus because has a potential has pharmacologist activity. The purpose of this research to investigate the role essential oil from Curcuma longa and Cymbopogon citratus through LOX gene. Several chemical substances, including 3,7-dimethyl-1,3,6-octatriene, camphor, eugenol, curzerene and isoborneol were retrivied from PubChem database. The PyRx 0.8 was used to minimize and convert the sdf file to pdb format file of ligands. Those compounds were predicted their interaction using STITCH online server. Ligands and protein were docked by HEX Cuda 8.0.0 program, 3D and 2D views were evaluated using Discovery studio ver.19.0.0 and LigPlot+ ver 2.2, respectively. We found fourteen amino acid residues from LOX which bound the chemical compounds. Those interaction was supported by hydrogen bond with variety energy binding. To sum up, the essential oil from Curcuma longa and Cymbopogon citratus has a potential function as inhibitor LOX by inhibiting fourteen active side of the LOX gene. 
Pengaruh Polifenol Mahkota Dewa Terhadap Proliferasi Sel dan Apoptosis pada Mencit Strain Balb/C yang Diinduksi Benzo(a) Pyrene (BaP) Theopilus W. Watuguly; Indranila KS; Pamela Mercy Papilaya; Edi Dharmana
MEDIA MEDIKA INDONESIANA 2013:MMI VOLUME 47 ISSUE 1 YEAR 2013
Publisher : MEDIA MEDIKA INDONESIANA

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Abstract

ABSTRACT The effect of mahkota dewa polyphenol in the cell proliferation and apoptosis in BaP induced Balb/c ratsBackground: The polyphenol of mahkota dewa (Phaleria macrocarpa [Scheff.] Boerl) has the potency as antioxidant and anticancer which can handle free radicals, but there has not been extensive research on this. This research is aimed to prove the role of mahkota dewa polyphenol in the cell proliferation inhibition and induct lung carcinogenesis apoptosis in strain Balb/c mice which inducted with benzo(a)pyrene (BaP).Methods: Posttest control group design was carried out among 40 strain Balb/c mice sample, aged 1-2 weeks, weighed 20-30 grams, healthy mice condition. All mice were inducted with BaP and then randomized into 2 groups, as control group and the treatment group. The development of the lung tumor was observed by tissue surgery in the 8th, 17th and 26th week. The data collected were AgNORs, and IHC-TUNEL-apoptosis index dying. The data analysis was conducted using Kruskal-Wallis, Mann-Whitney, One-way ANOVA, and Post hoc test LSD with significance degree of p<α (0.05).Results: The oral administration of mahkota dewa polyphenol showed significantly decreasing cell proliferation, increasing apoptosis index in treatment group in week 8, 17 and 26 (p=0.000). Carcinogenesis incidence for the treatment group week 8 and 26 were 2.32±0.26 and 3.93±0.46, while for the treatment group were 1.88±0.38 and 0.88±0.22 (p=0.000). The cell proliferations for control group week 8 and 26 were 1.57±0.12 and 2.29±0.15, while for the treatment group were 1.53±0.11 and 1.60±0.04 (p=0.000). Apoptosis index for the control group for week 8 was 0.00±0.00 and 0.92±0.22 in week 26, while the treatment group was 1.12±0.71 and 2.02±1.05 (p=0.000).Conclusion: The administration of mahkota dewa polyphenol effectively inhibited the cell proliferation activity and increased apoptosis measured by apoptosis index. Therefore polyphenol has anticancer and antioxidant activities which can inhibit lung carcinogenesis in Balb/c mice.Keywords: Polyphenol, mahkota dewa (Phaleria macrocarpa [Scheff.] Boerl), apoptosis index, strain Balb/c mice, benzo(a)pyrene (BaP).ABSTRAKLatar belakang: Polifenol mahkota dewa (Phaleria macrocarpa [Scheff.] Boerl) berpotensi sebagai antioksidan dan antikanker yang mampu menangkap radikal bebas, namun belum banyak diteliti. Penelitian ini bertujuan membuktikan peran polifenol mahkota dewa dalam menginhibisi proliferasi sel dan menginduksi apoptosis pada mencit strain Balb/c hasil induksi Benzo(a)pyrene (BaP).Metode: Posttest control group design dengan sampel 40 mencit strain Balb/c, umur 1-2 minggu, berat 20-30 g, kondisi mencit sehat. Semua mencit diinduksi BaP kemudian hewan dirandomisasi menjadi 2 kelompok yaitu kelompok kontrol dan kelompok perlakuan polifenol 50 mg. Perkembangan tumor paru diamati dengan pembedahan jaringan pada minggu ke-8, 17 dan 26. Data dikumpulkan meliputi AgNORs, pengecatan IHC-TUNEL-indeks apoptosis. Analisis data dengan Kruskal-Wallis, Mann-Whitney, One-way ANOVA, post hoc test LSD dengan derajat kemaknaan p<α (0,05).Hasil: Pemberian oral polifenol mahkota dewa sebesar 50 mg secara bermakna memperlihatkan penurunan insidens karsinogenesis paru, proliferasi sel, protein Bax dan peningkatan indeks apoptosis, protein p53, Bcl-2, ekspresi caspase 3, 8, 9 pada kelompok perlakuan pada minggu ke-8, 17 dan 26 (p=0,000). Insidens karsinogenesis untuk kelompok kontrol minggu ke-8, dan 26 sebesar 2,32±0,26 dan 3,93±0,46, sedangkan kelompok perlakuan sebesar 1,88±0,38 dan 0,88±0,22 (p=0,000). Proliferasi sel untuk kelompok kontrol minggu ke-8 dan 26 sebesar 1,57±0,12 dan 2,29±0,15, sedangkan kelompok perlakuan sebesar 1,53±0,11 dan 1,60±0,04 (p=0,000). Indeks apoptosis pada kelompok kontrol minggu ke-8 sebesar 0,00±0,00 dan 0,92±0,22 minggu ke-26, sedangkan kelompok perlakuan sebesar 1,12±0,71 dan 2,02±1,05 (p=0,000).Simpulan: Pemberian polifenol mahkota dewa efektif meng-inhibisi proliferasi sel dan menginduksi apoptosis melalui peningkatan indeks apoptosis. Jadi polifenol mahkota dewa memiliki aktivitas antikanker dan antioksidan mampu menghambat karsinogenesis paru mencit Balb/c.
Induksi Polifenol Mahkota Dewa dan Apoptosis Sel Kanker Paru Mencit Strain Balb/C: Analisis pada Up-Regulation Bax dan Down-Regulation Bcl-2 Theopilus W Watuguly; Tjahjono Tjahjono; Martha Kaihena; Syahran Wael
MEDIA MEDIKA INDONESIANA 2012:MMI VOLUME 46 ISSUE 1 YEAR 2012
Publisher : MEDIA MEDIKA INDONESIANA

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Abstract

Mahkota Dewa polyphenol induction and apoptosis cell lung cancer in Balb/c strain mice analysis on up-regulation bax and downregulation Bcl-2Background: This research is aimed to prove the role of Mahkota Dewa polyphenol in the up-regulation Bax and down-regulation in strain Balb/c mice which inducted with benzo(a)pyrene (BaP).Method: Post test control group design with 30 strain Balb/c mice sample, aged 1-2 weeks, weighed 20-30 grams, healthy mice condition. All mice inducted with BaP and then randomized into 2 groups, which were the control group and the treatment group (polyphenol 50mg). The development of the lung carcinogenesis was observed by tissue surgery in the 8th, 17th and 26th week. The data collected include expression examination Bax and Bcl-2. The data analysis was conducted by Kruskal-Wallis, Mann-Whitneyand correlation test of Spearman's with significance degree of p<a (0.05).Result: The oral administration of Mahkota Dewa polyphenol of 50 mg were significantly decreased the occurance of lung carcinogenesis through decreasing of protein Bax and increasing Bcl-2 in treatment group in week 8, 17 and 26 (p=0.000). Protein Bax for the control group for week 8 were 4.04±0.22 and 1.92±0.10 in week 26, while the treatment group were 5.96±0.32 and 4.68±0.22 (p=0.000). Protein Bcl-2 for the control group for week 8 were 5.80±0.32 and 9.64±0.26 in week 26, while the treatmentgroup were 5.12±0.22 and 7.38±0.21 (p=0.000). The Spearman correlation for Bax and Bcl-2 with significancy value of (p=0.000).Conclusion: The administration of Mahkota Dewa polyphenol of 50 mg effectively increased Bax protein expression and decreased Bcl-2 protein expression in mice Balb/c.Keywords: Polyphenol, Mahkota Dewa (Phaleria macrocarpa [Scheff.] Boerl), protein Bax, protein Bcl-2, strain Balb/c mice, benzo(a)pyrene (BaP)ABSTRAKLatar belakang: Penelitian ini bertujuan membuktikan peran polifenol Mahkota Dewa dalam meningkatkan protein Bax dan menurunkan protein Bcl-2 pada mencit strain Balb/c hasil induksi Benzo(a)pyrene (BaP).Metode: Post test only control group design dengan sampel 30 mencit strain Balb/c, umur 1-2 minggu, berat 20-30 gram, kondisi mencit sehat. Semua mencit diinduksi BaP kemudian hewan dirandomisasi menjadi 2 kelompok yaitu kelompok kontrol dan kelompok perlakuan polifenol 50 mg. Perkembangan karsinogenesis paru diamati dengan pembedahan jaringan pada minggu ke-8, 17 dan 26. Data dikumpulkan meliputi pemeriksaan ekspresi Bax dan Bcl-2. Analisis data dengan Kruskal-Wallis dilanjutkan denganuji Mann-Whitney dan uji korelasi Spearman dengan derajat kemaknaan p<a (0,05).Hasil: Pemberian oral polifenol Mahkota Dewa sebesar 50 mg secara bermakna memperlihatkan peningkatan protein Bax dan penurunan protein Bcl-2 pada kelompok perlakuan pada minggu ke-8, 17 dan 26 (p=0,000). Protein Bax pada kelompok kontrol minggu ke-8 sebesar 4,04±0,22 dan 1,92±0,10 minggu ke-26, sedangkan kelompok perlakuan sebesar 5,96±0,32 dan 4,68±0,22 (p=0,000) sedangkan protein Bcl-2 pada kelompok kontrol minggu ke-8 sebesar 5,80±0,32 dan 9,64±0,26 minggu ke-26, sedangkan kelompok perlakuan sebesar 5,12±0,22 dan 7,38±0,21 (p=0,000). Hasil uji korelasi Spearman protein Bax dan protein Bcl-2 dengan nilai significancy (p=0,000).
Effect of Leaf Syzygium aromaticum on Lymphocytes and Macrophages Mice Balb/c Syahran Wael; Theopilus Wilhelmus Watuguly; Didik Wahyudi
Majalah Obat Tradisional Vol 23, No 2 (2018)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (642.224 KB) | DOI: 10.22146/mot.38474

Abstract

Syzygium aromaticum as an immunomodulator contains main active compound eugenol which is able to stimulate lymphocyte proliferation and the production of macrophages. Lymphocytes have a very important role to provide protection in the body against infection. This study aims to prove the effects of extract Syzygium aromaticum leaf against increased proliferation of lymphocytes, lymphoblast and macrophages of mice Balb/c of induced Salmonella typhimurium. The method used in this study was experimental with post test only control group. Mice Balb/c were divided into 4 groups as a control group and treatment induced of Salmonella typhimurium. The first treatment group were administrated extracts of 15mg /kgbw, the second treatment 75mg/kgbw, the third treatment of 150mg/kgbw for 12days. ANOVA test showed a significant difference in lymphocyte proliferation but not lymphoblast and macrophages.
UJI DAYA HAMBAT EKSTRAK ETANOL SURUHAN (Piperumia pellucida L.H.B Kunth) TERHADAP PERTUMBUHAN BAKTERI Staphylococcus aureus SECARA IN-VITRO Eldo Dandirwalu; Theopilus W Watuguly
BIOPENDIX Vol 2 No 1 (2015): Biopendix : Jurnal Biologi, Pendidikan & Terapan
Publisher : Program Studi Pendidikan Biologi FKIP Unpatti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biopendixvol2issue1page8-14

Abstract

Background: Staphyloccocus aureus represent normal mikroflora of human being. This bacterium usually there are at bronchi to the and husk. Existence of Staphyloccocus aurues at bronchi to the and husk at individual seldom cause disease, healthy individual usually only personating career. One of the original plant that is plant of suruhan (Piperumia pellucida L.H.B. Kunth) biggest and wide of in some area in Indonesia (Molluca) and also have potency to be developed as traditional drug, empirically have been used in medication of abscess (abses) by society. Methods: This Research use eksperimental in Laboratory with research device is Complete Random Device (Factorial RAL) Pattern from two factor and 3 restating, which in doing/conducting in Elementary Laboratory of Biology Faculty Teachership and Science Education of Pattimura University. Resulth: Result research of to extract etanol plant suruhan (Piperumia pelludica L.H.B Kunth) to growth of bacterium of Staphylococcus aureus with various concentration that is 25%, 50% and 75% and there is influence contact to growth of bacterium where at concentration 25% is equal to 5 mm donot give respon pursue, 50% is equal to 10 mm with weak resistance respon and 75% is equal to 16 with resistance respon. Conclusion: Extract concentration excelsior of etanol plant of suruhan (Piperumia pelludica L.H.B Kunth) and old progressively of contact with bacterium of Staphylococcus aureus hence resistivity progressively goodness
EFEKTIVITAS EKSTRAK BIJI PANGI (Pangium edule Reinw) TERHADAP MORTALITAS LARVA NYAMUK Anopheles Viesta Sampe; Theopilus Watuguly
BIOPENDIX Vol 2 No 2 (2016): Biopendix : Jurnal Biologi, Pendidikan & Terapan
Publisher : Program Studi Pendidikan Biologi FKIP Unpatti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biopendixvol2issue2page152-159

Abstract

Background: Eradication of mosquito nests in Maluku is still done by fogging and with other chemicals. But on the other hand can cause disease in the respiratory tract and environmental damage. Pangi seeds contain alkaloids and potentially saponin compounds as larvacide to kill mosquito larvae. Method: The study design was using RAL (Completely Randomized Design) with 5 treatments ie K1 (control), K2 0,04%, K3 0,08%, K4 0,16% and K5 0,23% with each 25 larvae with 3 repetitions observed for 24 hours. One Way ANOVA Test Result and Probit LC50 Analysis were used to find out the extract of pangi seed which is effective in killing mosquitoes. Result: One Way ANOVA test result obtained Fcount value is 467.063 with Sig value. 0.000 and probit analysis obtained 24 hours LC50 value of 0.053% means that the extract of pangi seed (Pangium edule Reinw.) Is effective against mortality of Anopheles mosquito larvae. Conclusion: Seed extract (Pangium edule Reinw) effect on mortality of Anopheles mosquito larvae with LC50 extract value of Pangi (Pangium edule Reinw) in 24 hours was 0.053%. Each concentration of pangi seed extract (Pangium edule Reinw) had an effect on mortality of Anopheles mosquito larvae within 24 hours of observation time. There was a correlation between various concentrations of Pangi (Pangium edule Reinw) with the mortality of Anopheles mosquito larvae with value (p) = 0.000 and the relationship level was very strong with Pearson correlation value 0.891.
PAPARAN EKSTRAK TERIPANG PASIR (Holothuria scabra) TERHADAP GAMBARAN HISTOPATOLOGI HATI MENCIT (Mus musculus) Yosep Matruty; Theopilus Watuguly
BIOPENDIX Vol 2 No 2 (2016): Biopendix : Jurnal Biologi, Pendidikan & Terapan
Publisher : Program Studi Pendidikan Biologi FKIP Unpatti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biopendixvol2issue2page160-169

Abstract

Background: In Indonesia, traditionally sea cucumber (Holothuria scabra) is often used as a cure gout. The purpose of the research is to determine the effects of sea cucumber (Holothuria scabra) extracts against liver histopathology picture mice (Mus musculus). Method: Mice used were male mice as much as 15 tails and were divided into 5 groups. Each group consisted of three mice. Group I as a positive control given allopurino l0.1%. The second group was given distilled water as a negative control. Group III, IV and V as the treatment group was given sea cucumber extract with each concentration of 5%, a concentration of 10% and 15% concentration. Result: The results were analyzed quantitatively, showed that the administration of potassium bromate showed the presence of fatty and necrosis. But after being given a sea cucumber extract with a concentration of 10% and a concentration of 15% still showed the presence of inflammatory cells and remnants of fatty liver (same as the picture of liver histopathology in the positive control). At a concentration of 5% normal liver histopathology same as the picture of liver histopathology in the negative control. Conclution: Sea cucumbers with a concentration of 5% can be used as a gout medicine without any side effects for conditions onal liver function.
ANALISIS SENYAWA FLAVONOID DAUN LAMUN Enhalus acoroides DI PERAIRAN PANTAI DESA WAAI KABUPATEN MALUKU TENGAH Icak Darling Rahakbauw; Theopilus Watuguly
BIOPENDIX Vol 3 No 1 (2016): Biopendix : Jurnal Biologi, Pendidikan & Terapan
Publisher : Program Studi Pendidikan Biologi FKIP Unpatti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biopendixvol3issue1page53-62

Abstract

Background: The coastal waters of Waai Village are the waters located in Kecamatan Kecamatan, Central Maluku District. The potential of coastal resources in this area is quite a lot of seagrass (Seagrass). Although quite a lot but because of the lack of information related to the existing seagrass so that until now the utilization of seagrass in the area has not been maximized. This study aims to determine the total flavonoid compounds contained in the seagrass of Enhalus acoroides. Method: This research was conducted at Basic Chemical Laboratory, Faculty of Mathematics and Natural Sciences (FMIPA) Pattimura University Ambon. The study was conducted from March 20-28, 2016, with sampling location in coastal waters of Waai Village, Central Maluku District. The method used refers to the Chang method by using AlCl3 reagent. Result: Based on total analysis of flavonoid compound using laboratory experimental method obtained the average flavonoid level contained in Enhalus acoroides seagrass leaves of 3.569%. Conclusion: Seaweed leaves Enhalus acoroides taken from coastal waters of Waai Village contain flavonoid compounds with an average value of 3.5697%.
EKSTRAK ETANOL TERIPANG PASIR (Holothuria scabra) DALAM MEMPERBAIKI KERUSAKAN USUS HALUS MENCIT (Mus Musculus) YANG TERPAPAR KALIUM BROMAT MELALUI PENGAMATAN GAMBARAN HISTOPATOLOGI MENCIT Hanna Kalorbobir; Theopilus Watuguly
BIOPENDIX Vol 3 No 2 (2017): Biopendix : Jurnal Biologi, Pendidikan & Terapan
Publisher : Program Studi Pendidikan Biologi FKIP Unpatti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biopendixvol3issue2page115-123

Abstract

Background: In Indonesia, traditionally sea cucumber (Holothuria scabra) is often used as a drug for uric acid. A study has been conducted on the effect of giving sandy cucumber extract (Holothuria scabra) to microscopic images of the small intestine of mice (Mus muculus) induced by potassium bromate. Method: This study used a small intestine sample of 2-3 months old male mice weighing + 20-30gr for 15 mice used in the study, divided by 5 groups, ie group I was given allopurinol as a positive control, group II was given aquades as control group negative, group III, IV and V were given extract of sea cucumber sand and potassium bromate as treatment group with concentration of 5%, 10%, and 15%. Result: The result of the research was analyzed quantitatively, showed that the extract of sea cucumber with 10% concentration was not significantly different with the extract of sea cucumber with concentration of 15%, when compared with the giving of sea cucumber extract with 5% concentration gave significantly different result with the giving of extract sea ​​cucumbers with concentrations of 10% and 15%. This shows the provision of sea cucumber extract with a concentration of 5% can give microscopic changes in the small intestine of mice due to exposure to potassium bromate. Conclusion: Sand cucumber extract (Holothuria scabra) with 5% concentration can give a change of histology picture of small intestine of mice (Mus muculus).
Co-Authors Aknes Tahya Alfia Sabban Anik Uniarti Bare, Yohanes Beatrixs Rupilu Chrismenda Pay Didik Wahyudi Dominggus Rumahlatu Edi Dharmana Eldo Dandirwalu F Leiwakabessy Febi Stevano Rumangun H Sinay Hanna Kalorbobir Harfalien Tehubijuluw Haslina Kole Icak Darling Rahakbauw Indahsari, Lilin Ika Nur Indranila KS Ine Arini Jecklyn A Lekal Johanis Fritzgal Rehena M Kaihena Maria Nindatu Martha Kaihena Nia Rusfita Titdoy Nindatu, Maria Nurmila Nurmila Pamela Mercy Papilaya Prelly M Tuapattinaya Prelly M.J Tuapattinaya Prelly Tuapattinaya R Malawat Rezza Julian Patty Sari, Dewi Ratih Tirto Silvia E Lopulalan Sintje Liline Sriyanti Imelda Aksamina Salmanu Syahran Wael Syahran Wael Syahran Wael Tjahjono Tjahjono Uniarti, Anik Viesta Sampe Yosep Matruty Yulviany Latuihamallo