Article Per Year (5 Year)
p-Index From 2019 - 2024
2.147
P-Index
This Author published in this journals
All Journal MEDIA MEDIKA INDONESIANA Majalah Obat Tradisional Jurnal Profesi Medika: Jurnal Kedokteran dan Kesehatan Biopendix: Jurnal Biologi, Pendidikan dan Terapan Science Map Journal JSMARTech : Journal of Smart Bioprospecting and Technology Bioscientist : Jurnal Ilmiah Biologi
Theopilus W Watuguly
Program Pendidikan Biologi, Jurusan PMIPA FKIP Dan Program Pendidikan Dokter Universitas Pattimura Ambon, Jl. Dr. Tamaela, Kampus Universitas Pattimura Ambon
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Education Health Professions Immunology & microbiology Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Physics Public Health Social Sciences

Published : 26 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 18 Documents
Search
Journal : Biopendix: Jurnal Biologi, Pendidikan dan Terapan

 1   2 
UJI DAYA HAMBAT EKSTRAK ETANOL SURUHAN (Piperumia pellucida L.H.B Kunth) TERHADAP PERTUMBUHAN BAKTERI Staphylococcus aureus SECARA IN-VITRO Eldo Dandirwalu; Theopilus W Watuguly
BIOPENDIX Vol 2 No 1 (2015): Biopendix : Jurnal Biologi, Pendidikan & Terapan
Publisher : Program Studi Pendidikan Biologi FKIP Unpatti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biopendixvol2issue1page8-14

Abstract

Background: Staphyloccocus aureus represent normal mikroflora of human being. This bacterium usually there are at bronchi to the and husk. Existence of Staphyloccocus aurues at bronchi to the and husk at individual seldom cause disease, healthy individual usually only personating career. One of the original plant that is plant of suruhan (Piperumia pellucida L.H.B. Kunth) biggest and wide of in some area in Indonesia (Molluca) and also have potency to be developed as traditional drug, empirically have been used in medication of abscess (abses) by society. Methods: This Research use eksperimental in Laboratory with research device is Complete Random Device (Factorial RAL) Pattern from two factor and 3 restating, which in doing/conducting in Elementary Laboratory of Biology Faculty Teachership and Science Education of Pattimura University. Resulth: Result research of to extract etanol plant suruhan (Piperumia pelludica L.H.B Kunth) to growth of bacterium of Staphylococcus aureus with various concentration that is 25%, 50% and 75% and there is influence contact to growth of bacterium where at concentration 25% is equal to 5 mm donot give respon pursue, 50% is equal to 10 mm with weak resistance respon and 75% is equal to 16 with resistance respon. Conclusion: Extract concentration excelsior of etanol plant of suruhan (Piperumia pelludica L.H.B Kunth) and old progressively of contact with bacterium of Staphylococcus aureus hence resistivity progressively goodness
EFEKTIVITAS EKSTRAK BIJI PANGI (Pangium edule Reinw) TERHADAP MORTALITAS LARVA NYAMUK Anopheles Viesta Sampe; Theopilus Watuguly
BIOPENDIX Vol 2 No 2 (2016): Biopendix : Jurnal Biologi, Pendidikan & Terapan
Publisher : Program Studi Pendidikan Biologi FKIP Unpatti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biopendixvol2issue2page152-159

Abstract

Background: Eradication of mosquito nests in Maluku is still done by fogging and with other chemicals. But on the other hand can cause disease in the respiratory tract and environmental damage. Pangi seeds contain alkaloids and potentially saponin compounds as larvacide to kill mosquito larvae. Method: The study design was using RAL (Completely Randomized Design) with 5 treatments ie K1 (control), K2 0,04%, K3 0,08%, K4 0,16% and K5 0,23% with each 25 larvae with 3 repetitions observed for 24 hours. One Way ANOVA Test Result and Probit LC50 Analysis were used to find out the extract of pangi seed which is effective in killing mosquitoes. Result: One Way ANOVA test result obtained Fcount value is 467.063 with Sig value. 0.000 and probit analysis obtained 24 hours LC50 value of 0.053% means that the extract of pangi seed (Pangium edule Reinw.) Is effective against mortality of Anopheles mosquito larvae. Conclusion: Seed extract (Pangium edule Reinw) effect on mortality of Anopheles mosquito larvae with LC50 extract value of Pangi (Pangium edule Reinw) in 24 hours was 0.053%. Each concentration of pangi seed extract (Pangium edule Reinw) had an effect on mortality of Anopheles mosquito larvae within 24 hours of observation time. There was a correlation between various concentrations of Pangi (Pangium edule Reinw) with the mortality of Anopheles mosquito larvae with value (p) = 0.000 and the relationship level was very strong with Pearson correlation value 0.891.
PAPARAN EKSTRAK TERIPANG PASIR (Holothuria scabra) TERHADAP GAMBARAN HISTOPATOLOGI HATI MENCIT (Mus musculus) Yosep Matruty; Theopilus Watuguly
BIOPENDIX Vol 2 No 2 (2016): Biopendix : Jurnal Biologi, Pendidikan & Terapan
Publisher : Program Studi Pendidikan Biologi FKIP Unpatti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biopendixvol2issue2page160-169

Abstract

Background: In Indonesia, traditionally sea cucumber (Holothuria scabra) is often used as a cure gout. The purpose of the research is to determine the effects of sea cucumber (Holothuria scabra) extracts against liver histopathology picture mice (Mus musculus). Method: Mice used were male mice as much as 15 tails and were divided into 5 groups. Each group consisted of three mice. Group I as a positive control given allopurino l0.1%. The second group was given distilled water as a negative control. Group III, IV and V as the treatment group was given sea cucumber extract with each concentration of 5%, a concentration of 10% and 15% concentration. Result: The results were analyzed quantitatively, showed that the administration of potassium bromate showed the presence of fatty and necrosis. But after being given a sea cucumber extract with a concentration of 10% and a concentration of 15% still showed the presence of inflammatory cells and remnants of fatty liver (same as the picture of liver histopathology in the positive control). At a concentration of 5% normal liver histopathology same as the picture of liver histopathology in the negative control. Conclution: Sea cucumbers with a concentration of 5% can be used as a gout medicine without any side effects for conditions onal liver function.
ANALISIS SENYAWA FLAVONOID DAUN LAMUN Enhalus acoroides DI PERAIRAN PANTAI DESA WAAI KABUPATEN MALUKU TENGAH Icak Darling Rahakbauw; Theopilus Watuguly
BIOPENDIX Vol 3 No 1 (2016): Biopendix : Jurnal Biologi, Pendidikan & Terapan
Publisher : Program Studi Pendidikan Biologi FKIP Unpatti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biopendixvol3issue1page53-62

Abstract

Background: The coastal waters of Waai Village are the waters located in Kecamatan Kecamatan, Central Maluku District. The potential of coastal resources in this area is quite a lot of seagrass (Seagrass). Although quite a lot but because of the lack of information related to the existing seagrass so that until now the utilization of seagrass in the area has not been maximized. This study aims to determine the total flavonoid compounds contained in the seagrass of Enhalus acoroides. Method: This research was conducted at Basic Chemical Laboratory, Faculty of Mathematics and Natural Sciences (FMIPA) Pattimura University Ambon. The study was conducted from March 20-28, 2016, with sampling location in coastal waters of Waai Village, Central Maluku District. The method used refers to the Chang method by using AlCl3 reagent. Result: Based on total analysis of flavonoid compound using laboratory experimental method obtained the average flavonoid level contained in Enhalus acoroides seagrass leaves of 3.569%. Conclusion: Seaweed leaves Enhalus acoroides taken from coastal waters of Waai Village contain flavonoid compounds with an average value of 3.5697%.
EKSTRAK ETANOL TERIPANG PASIR (Holothuria scabra) DALAM MEMPERBAIKI KERUSAKAN USUS HALUS MENCIT (Mus Musculus) YANG TERPAPAR KALIUM BROMAT MELALUI PENGAMATAN GAMBARAN HISTOPATOLOGI MENCIT Hanna Kalorbobir; Theopilus Watuguly
BIOPENDIX Vol 3 No 2 (2017): Biopendix : Jurnal Biologi, Pendidikan & Terapan
Publisher : Program Studi Pendidikan Biologi FKIP Unpatti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biopendixvol3issue2page115-123

Abstract

Background: In Indonesia, traditionally sea cucumber (Holothuria scabra) is often used as a drug for uric acid. A study has been conducted on the effect of giving sandy cucumber extract (Holothuria scabra) to microscopic images of the small intestine of mice (Mus muculus) induced by potassium bromate. Method: This study used a small intestine sample of 2-3 months old male mice weighing + 20-30gr for 15 mice used in the study, divided by 5 groups, ie group I was given allopurinol as a positive control, group II was given aquades as control group negative, group III, IV and V were given extract of sea cucumber sand and potassium bromate as treatment group with concentration of 5%, 10%, and 15%. Result: The result of the research was analyzed quantitatively, showed that the extract of sea cucumber with 10% concentration was not significantly different with the extract of sea cucumber with concentration of 15%, when compared with the giving of sea cucumber extract with 5% concentration gave significantly different result with the giving of extract sea ​​cucumbers with concentrations of 10% and 15%. This shows the provision of sea cucumber extract with a concentration of 5% can give microscopic changes in the small intestine of mice due to exposure to potassium bromate. Conclusion: Sand cucumber extract (Holothuria scabra) with 5% concentration can give a change of histology picture of small intestine of mice (Mus muculus).
POTENSI EKSTRAK DAUN TERATAI (Nymphaea pubescens L.) DALAM MENGHAMBAT Staphylococcus aureus Alfia Sabban; Dominggus Rumahlatu; Theopilus Watuguly
BIOPENDIX Vol 3 No 2 (2017): Biopendix : Jurnal Biologi, Pendidikan & Terapan
Publisher : Program Studi Pendidikan Biologi FKIP Unpatti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biopendixvol3issue2page129-141

Abstract

Background: The lotus (Nymphaea pubescens L.) is one of the ornamental plants that many people in demand because it has varieties, shapes, and color of flowers are diverse. The lotus has the potential to be developed as an antibacterial. This study aims to determine the use of lotus leaf extract as anti-bacterial Staphylococcus aureus. Method: This study included the making of extraction using maceration method using ethanol, phytochemical testing of lotus leaf extract and testing of anti bacterial activity was done by diffusion method agar by observing and measuring the inhibition zone diameter formed on Muller Hinton (MHA) media. Then done by giving lotus leaf extract with 3 treatment of concentration that is 5%, 10%, 20%, positive control (ampicillin) and negative control (aquades). With an incubation period of 1 x 24 hours. Result: The result of phytochemical test showed that lotus leaf extract contain alkaloid compound, triterpenoid, steroid, flavonoid, phenolic and saponin. Anti-bacterial activity test results showed that the concentration given was not able to inhibit the growth of test bacteria. Conclusions: Bacteriostatic tests with a 60% extract concentration indicating that at 10-5 to 10-9 dilutions there were no bacterial colonies growing.
ANALISIS KANDUNGAN FLAVONOID PADA TEH BENALU (Dendropohtoe pentandra (L.) Miq.) Jecklyn A Lekal; Theopilus Watuguly
BIOPENDIX Vol 3 No 2 (2017): Biopendix : Jurnal Biologi, Pendidikan & Terapan
Publisher : Program Studi Pendidikan Biologi FKIP Unpatti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biopendixvol3issue2page154-158

Abstract

Background: Benalu is one of the plant species that lives attached to other plants. The parasite is hemiparasit or half parasitic because it has a green leaf substance (chlorophyll) used for the assimilation process and only sucks water and organic matter from its host plant. Flavonoids are phenol compounds commonly found in vascular plants including parasites. Flavonoids are useful for protecting cell structures, increasing the effectiveness of vitamin C, anti-inflammation, preventing bone loss and as an antibiotic. Method: Total flavonoid content in clove parasite tea using UV-Vis Spectrophotometer method. Results: Total flavonoid in tea clove parasite was 0.2819%. Flavonoids found in tea parasite cloves are low with the presence of heating and temperature factors. Conclusion: Based on the quantitative test, leaf parasite is the highest flavonoid level of 13.702% and after being made tea, flavonoid levels changed to 0.281%.
UJI KELIMPAHAN BAKTERI COLIFORM PADA MAKANAN JAJANAN BAKSO TUSUK YANG DIJUAL DI LINGKUNGAN SDN 82 KUDAMATI DAN SDN 2 TANAH TINGGI AMBON Aknes Tahya; M Kaihena; Theopilus Watuguly
BIOPENDIX Vol 4 No 2 (2018): Biopendix : Jurnal Biologi, Pendidikan & Terapan
Publisher : Program Studi Pendidikan Biologi FKIP Unpatti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biopendixvol4issue2page97-101

Abstract

Background: Snack food is part of food that is inseparable from the community. Snack food consists of various types, one of which is skewer meatballs. Skewers are a type of snack food made from flour and meat that is round and then boiled until cooked, has a savory and chewy taste. One of the bacteria that often contaminates snacks is coliform bacteria. Coliform bacteria is a group of rod-shaped gram negative bacteria. Method: The sample used in the study was skewered meatball samples taken at the location of SDN 82 Kudamati and SDN 2 Tanah Tinggi Ambon. Testing for the presence of coliform bacteria was carried out at the Indonesian Institute of Research and Standardization Laboratory in Ambon. The type of research used is descriptive. Calculation of the number of bacterial colonies using the standard Total Plate Count (TPC). Results: The study showed that the two positive samples contained coliform bacteria with the highest number of coliform bacteria colonies in code A1 1.55 x 105 at location A SDN 82 Kudamati and the lowest at code B1 1.49 x 103 at location B SDN 2 Tanah Tinggi. If compared with SNI for processed meat and chicken products is 1 x 105 colonies / gram, which means the highest number of colonies cannot be consumed. Conclusion: Skewers meatball snacks sold in SDN 82 Kudamati and Ambon Tanah 2 Elementary School 2, positive for coliform bacteria
ANALISIS KADAR FLAVONOID PADA TEH DAUN LAMUN (Enhalus acoroides) BERDASARKAN TINGKAT KETUAAN DAUN Harfalien Tehubijuluw; Theopilus Watuguly; Prelly M.J Tuapattinaya
BIOPENDIX Vol 5 No 1 (2018): Biopendix : Jurnal Biologi, Pendidikan & Terapan
Publisher : Program Studi Pendidikan Biologi FKIP Unpatti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biopendixvol5issue1page1-7

Abstract

Background: Seagrass (Enhalus acoroides) is a flowering plant (Angiospermae) that has fully adapted to life immersed in the sea. Seagrass is also a commodity that has been used by many people both modern and traditional. Traditionally seagrass has been used for compost and fertilizer, cigars and children's toys, made into baskets, some are eaten, and made into fishing nets. Whereas in the modern way is as a filter of waste, food, medicines, materials for paper mills, and sources of chemicals. Methods: Measurement of flavonoid levels of seagrass leaf tea (Enhalus acoroides) was analyzed using a one-way anava test. This research was conducted on 10-18 July 2018. Results: The analysis showed that old tea drinks from seagrass leaves (Enhalus acoroides) had high flavonoid levels of 0.1623% while half-old tea drinks with seagrass leaves had moderate flavonoid levels of 0.1263%. Conclusion: Young tea drinks using seagrass leaves (Enhalus acoroides) have lower flavonoid levels of 0.0888%
STUDI PEMANFAATAN TUMBUHAN OBAT TRADISIONAL OLEH MASYARAKAT SUKU OIRATA PULAU KISAR KECAMATAN PULAU-PULAU TERSELATAN KABUPATEN MALUKU BARAT DAYA Beatrixs Rupilu; Theopilus Watuguly
BIOPENDIX Vol 5 No 1 (2018): Biopendix : Jurnal Biologi, Pendidikan & Terapan
Publisher : Program Studi Pendidikan Biologi FKIP Unpatti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biopendixvol5issue1page53-64

Abstract

Background: Medicinal plants are medicinal plants that can relieve pain, increase endurance and treat various diseases. The Oirata tribe is an indigenous tribe of Kisar Island. The community traditionally uses various types of plants as alternative treatments to cure diseases because they are easier to obtain and there are no side effects. Methods: This study uses descriptive methods. Data collection techniques by conducting interviews, observation, questionnaires, and collecting and documenting medicinal plants. Results: Medicinal plants found in the Oirata Tribe of Kisar Island, the Sub district Islands, Southwest Maluku Regency are 31 types of medicinal plants. Plant parts used are roots, bark, leaves, flowers, fruits and seeds. How to process traditional medicinal plants is done by boiling, pounding, chopping, squeezing, pasting, wrapping, heating, and some even eaten directly to swallow the water. Conclusion: The way to use it for the people of the Oirata tribe is that it is single because they do not use plants mixed with plants or other raw materials only mixed with water.
Co-Authors Aknes Tahya Alfia Sabban Anik Uniarti Bare, Yohanes Beatrixs Rupilu Chrismenda Pay Didik Wahyudi Dominggus Rumahlatu Edi Dharmana Eldo Dandirwalu F Leiwakabessy Febi Stevano Rumangun H Sinay Hanna Kalorbobir Harfalien Tehubijuluw Haslina Kole Icak Darling Rahakbauw Indahsari, Lilin Ika Nur Indranila KS Ine Arini Jecklyn A Lekal Johanis Fritzgal Rehena M Kaihena Maria Nindatu Martha Kaihena Nia Rusfita Titdoy Nindatu, Maria Nurmila Nurmila Pamela Mercy Papilaya Prelly M Tuapattinaya Prelly M.J Tuapattinaya Prelly Tuapattinaya R Malawat Rezza Julian Patty Sari, Dewi Ratih Tirto Silvia E Lopulalan Sintje Liline Sriyanti Imelda Aksamina Salmanu Syahran Wael Syahran Wael Syahran Wael Tjahjono Tjahjono Uniarti, Anik Viesta Sampe Yosep Matruty Yulviany Latuihamallo